74 pubblicazioni classificate nel seguente modo:

Development of activated carbons derived from wastes: coffee grounds and olive stones as potential porous materials for air depollution
SCIENCE OF THE TOTAL ENVIRONMENT
Autore/i: Czerwinska, Natalia; Giosue', Chiara; Matos, Ines; Sabbatini, Simona; Ruello, Maria Letizia; Bernardo, Maria
Classificazione: 1 Contributo su Rivista
Abstract: Agro-industrial byproducts and food waste necessitate an environmentally friendly way of reducing issues related to their disposal; it is also necessary to recover as much new raw material from these resources as possible, especially when we consider their potential usage as a precursor for preparing depolluting materials, such as activated carbon. In this work, coffee grounds and olive stones were chosen as precursors and the adsorption capacity of the obtained porous carbons for volatile organic compounds (VOCs) was studied. Microporous activated carbons (ACs) were prepared using chemical (K2CO3) and physical (CO2) activation. The influence of the activation process, type, and time of activation was also investigated. Measurements of VOCs adsorption were performed, and methyl-ethyl-ketone (MEK) and toluene were chosen as the model pollutants. The surface areas and total pore volumes of 1487 m2/g and 0.53 cm3/g and 870 m2/g and 0.22 cm3/g for coffee ground carbons and olive stone carbons, respectively, were obtained via chemical activation, whereas physical activation yielded values of 716 m2/g and 0.184 cm3/g and 778 cm2 g-1 and 0.205 cm3/g, respectively. As expected, carbons without activation (biochars) showed the smallest surface area, equal to 331 m2/g and 251 m2/g, and, hence, the lowest adsorption capacity. The highest adsorption capacity of MEK (3210 mg/g) and toluene (2618 mg/g) was recorded for chemically activated coffee grounds. Additionally, from the CO2 isotherms recorded at a low pressure (0.03 bar) and 0 °C, the maximum CO2 adsorption capacity was equal to 253 mg/g.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/328331

Waste-Valorized Nanowebs for Crystal Violet Removal from Water
SMALL SCIENCE
Autore/i: Maqbool, Qaisar; Cavallini, Isabella; Lasemi, Niusha; Sabbatini, Simona; Tittarelli, Francesca; Rupprechter, Gunther
Classificazione: 1 Contributo su Rivista
Abstract: Lightweight, metal-free, sustainable, and reusable adsorbent materials are of paramount significance in addressing the challenges of wastewater treatment. Accordingly, semi-crystalline nanocellulose (NC) is extracted from tissue paper waste and used to modify polyacrylonitrile (PAN) to produce electrospun nanowebs with strand diameters from ≈180–300 nm. The incorporation of NC into PAN is confirmed by infrared and Raman spectroscopy and X-Ray diffraction. When tested for crystal violet (CV) adsorption, NC-modified PAN (20% NC@PAN) exhibits the highest CV removal capacity, achieving 91–94% removal over three cycles each, demonstrating exceptional recyclability. In contrast, unmodified PAN significantly decreases in CV adsorption capacity (from 59% to 48% in the third cycle), possibly due to an increased (≈36%) nanofiber diameter. The adsorption kinetics, exhibiting pseudo-second order, interparticle (in between nanofibers) diffusion, and Elovich kinetic models emphasize the role of multilayer CV adsorption through reversible chemical interactions. Confocal micro-Raman spectroscopy unveils a multifaceted CV adsorption mechanism, suggesting both surface and multilayer diffusion, with NC-enhancing interactions. These findings demonstrate the potential of NC-modified PAN nanowebs as effective and environmentally sustainable adsorbents for removing CV from aqueous solutions, suggesting promising practical applications.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/326631

Density functional theory and molecular dynamics studies on electrical, mechanical, and thermal properties of TiO2 nanoparticles interacting with poly lactic-co-glycolic acid
COLLOIDS AND SURFACES. A, PHYSICOCHEMICAL AND ENGINEERING ASPECTS
Autore/i: Laudadio, Emiliano; Mohebbi, Elaheh; Pavoni, Eleonora; Minnelli, Cristina; Sabbatini, Simona; Stipa, Pierluigi
Classificazione: 1 Contributo su Rivista
Abstract: Poly-lactic poly-co-glycolic acid (PLGA) copolymer is widely used thanks to its biocompatibility, even if could be difficult to find desirable mechanical properties for the polymer matrix. For this reason, inorganic nanoparticles like titanium dioxide (TiO2) can be used to modulate the mechanical properties of systems maintaining their biological capabilities. In this concern, density functional theory (DFT) and molecular dynamics (MD) simulations have been used to study the absorption phenomena of PLGA on surfaces of I4₁/amd (Anatase), P42/mnm (Rutile), and Pbca (Brookite) polymorphs of TiO2 DFT results showed that Anatase TiO2 is the best choice to absorb PLGA in comparison to the other PLGA-TiO2 surfaces. MD simulations remarked a peculiar stabilization pathway of PLGA-TiO2 with Anatase surface along the MD time and the largest increase in both Young’s and Bulk’s moduli. Finally, the influence of temperature on the mechanical properties of PLGA/A-TiO2 has also been investigated; in particular, MD results revealed that Young’s modulus of nanocomposite and the binding energy decreased by increasing the temperature. The outcomes of the present investigation could be helpful to clarify the potential uses of the PLGA-TiO2 nanocomposites in the biomedical field ranging from bone tissue engineering to drug delivery.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/313852

Insights into PLGA-encapsulated epigallocatechin 3-gallate nanoparticles as a new potential biomedical system: A computational and experimental approach
EUROPEAN POLYMER JOURNAL
Autore/i: Minnelli, Cristina; Stipa, Pierluigi; Sabbatini, Simona; Mengucci, Paolo; Mobbili, Giovanna; Galeazzi, Roberta; Armeni, Tatiana; Romaldi, Brenda; Celli, Annamaria; Laudadio, Emiliano
Classificazione: 1 Contributo su Rivista
Abstract: Biodegradable poly-lactic poly-glycolic copolymer (PLGA) represents one of the best strategies adopted to convey small molecules unstable and characterised by a low bioavailability if administered without vehicles. This is the case of Epigallocatechin-3-gallate (EGCG), which is one of the most interesting polyphenols from a biomedical point of view. In this study, the impact of EGCG on PLGA nanoparticles (NPs) properties has been studied underlining the role of the binary PVA/Poloxamer-407 as stabilizer system for obtaining colloidal stable NPs. Characterization has been carried out by dynamic light scattering (DLS), encapsulation efficiency, drug release studies, scanning electron microscopy (SEM) and infrared spectroscopy (IR). Molecular dynamics (MD) simulations have been used to model the PLGA and EGCG systems to understand the behaviour of NPs and the interactions that guided the EGCG encapsulation. The nanodispersions exhibited promising cytotoxic potentials in human lung carcinoma A549 cell lines enhancing the well-known growth inhibitor effect of the free EGCG.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/309751

In vitro toxicity of short vs long chrysotile fibres
PERIODICO DI MINERALOGIA
Autore/i: Gualtieri, Alessandro; Mirata, Serena; Almonti, Vanessa; Maria Bassi, Anna; Meo, Chiara; Scarfì, Sonia; Zapparoli, Mauro; Armeni, Tatiana; Cianfruglia, Laura; Marzioni, Daniela; Fantone, Sonia; Tossetta, Giovanni; Stipa, Pierluigi; Laudadio, Emiliano; Sabbatini, Simona; Minnelli, Cristina; DI VALERIO, Silvia; Vaiasicca, Salvatore; Procopio, Antonio; Pugnaloni, Armanda
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/323471

Cytotoxicity in vitro of two non-commercial samples of chrysotile and fibrous erionite
PERIODICO DI MINERALOGIA
Autore/i: Pugnaloni, Armanda; DI VALERIO, Silvia; Armeni, Tatiana; Cianfruglia, Laura; Tiano, Luca; Marchegiani, Fabio; Stipa, Pierluigi; Laudadio, Emiliano; Sabbatini, Simona; Minnelli, Cristina; Vaiasicca, Salvatore; Ramini, Debora; Procopio, Antonio D.; Fazioli, Francesca; Francesco Gualtieri, Alessandro
Classificazione: 1 Contributo su Rivista
Abstract: Exposure to naturally occurring asbestos and fibrous erionite can have severe impacts for the human health and cause malignant mesothelioma. The disease burden associated with this exposure is difficult to quantify and is not properly investigated. A characterization of the crystal-chemical-physical properties and toxicity/carcinogenicity of naturally occurring fibres with attention to asbestos and erionite is therefore highly recommended. The present study deals with the in vitro biological effects in MeT5A and A549 human cell lines of two non-commercial asbestos fibres: Valmalenco chrysotile from Central Alps Italy and fibrous erionite from Jersey Nevada, USA, including the standards UICC Chrysotile and UICC crocidolite. Light and scanning electron microscopy (SEM) observations showed morphological cell perturbation of treated cells. SEM-EDAX investigations revealed that the release of chemical components in the culture media affect cell viability. ROS levels increased in accordance with glutathione depletion, witnessing a remarkable cellular stress. Free radical release on pristine fibres was investigated by means of electron paramagnetic resonance (EPR) spin trapping technique and FTIR spectroscopy to characterize the molecular structures of fibres. Results reflect the cytotoxic effects and evidence the different toxicity pathways prompted by these fibre species due to their different crystal assemblages and physical properties.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/323351

In vitro toxicity of short vs long chrysotile fibres
Autore/i: Gualtieri, Alessandro; Mirata, Serena; Almonti, Vanessa; Bassi Anna, Maria; Meo, Chiara; Scarfì, Sonia; Zapparoli, Mauro; Armeni, Tatiana; Cianfruglia, Laura; Marzioni, Daniela; Fantone, Sonia; Tossetta, Giovanni; Stipa, Pierluigi; Laudadio, Emiliano; Sabbatini, Simona; Minnelli, Cristina; DI VALERIO, Silvia; Vaiasicca, Salvatore; Procopio, Antonio; Pugnaloni, Armanda
Classificazione: 1 Contributo su Rivista
Abstract: Chrysotile is a natural hydrous layer silicate that belongs to the serpentine group. Because of its unique asbestiform shape and outstanding physical-chemical properties, chrysotile is still nowadays the most used commercial mineral fibre in the world. This occurs despite the mineral has been classified as a Group 1 carcinogen by the International Agency for Research on Cancer (IARC). Since the “fibre toxicity paradigm” relies on the fibre size (length L and width W) and biodurability, the present work attempts to shed light on the issue of the toxicity of short vs. long chrysotile fibres by investigating two size-separated batches of chrysotile fibres (L>5 mm and L≤5 mm) of a commercial Russian sample. In parallel, UICC crocidolite and NYAD G wollastonite fibres were used as a positive and a negative carcinogenic standard, respectively. The calculated fibre potential toxicity/pathogenicity index (FPTI) value of the long-fibre chrysotile (2.35) is greater than that of the short-fibre (2.18) predicting a greater potential of toxicity/pathogenicity of the former. The FTPI value of short-fibre chrysotile is greater than that of wollastonite (1.92-2.12) but lower than that of crocidolite (2.67). The theoretical predictions were complemented with in vitro toxicity tests performed in parallel on four different cell types involved in the toxicity of inhaled mineral fibres: alveolar, endothelial, mesothelial cells and macrophages. The results from the MTT and LDH toxicity and ROS production tests are in line with the FPTI prediction. The negative carcinogenic standard wollastonite displays the lower in vitro toxicity score (0.25), while the short-fibre chrysotile presents a higher score (0.43), followed by the long-fibre chrysotile (0.66) and the positive carcinogenic standard crocidolite (0.67).In conclusion, our in vitro acute toxicity results on chrysotile are in line with the FPTI model predictions, indicating that short chrysotile asbestos fibres should not be assimilated to “innocuous dusts”

Development of a Quartz-Based Photo-Mobile Polymer Film for Controlled Motion Triggered by Light or Heat
MATERIALS
Autore/i: Castagna, R.; Riminesi, C.; Pianesi, M. S.; Sabbatini, S.; Di Donato, A.; Singh, G.; Francescangeli, O.; Cantisani, E.; Castellini, P.; Lucchetta, D. E.
Classificazione: 1 Contributo su Rivista
Abstract: We have developed a photo-mobile polymer film, that combines organic and inorganic materials, to allow for controlled motion that can be triggered by light or heat. Our film is made using recycled quartz and consists of two layers: a multi-acrylate polymer layer and a layer containing oxidized 4-amino-phenol and N-Vinyl-1-Pyrrolidinone. The use of quartz in our film also gives it a high temperature resistance of at least 350 °C. When exposed to heat, the film moves in a direction that is independent of the heat source, due to its asymmetrical design. Once the heat source is removed, the film returns to its original position. ATR-FTIR measurements confirm this asymmetrical configuration. This technology may have potential applications in energy harvesting, due to the piezoelectric properties of quartz.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/316313

Integration of Lipid-Functionalized Epigallocatechin-3-gallate into PLGA Matrix as a Novel Polyphenol-Based Nanoantioxidant
ACS OMEGA
Autore/i: Minnelli, Cristina; Stipa, Pierluigi; Mobbili, Giovanna; Sabbatini, Simona; Romaldi, Brenda; Armeni, Tatiana; Laudadio, Emiliano
Classificazione: 1 Contributo su Rivista
Abstract: The search for polyphenol-based materials with antioxidant activity is a growing research area in the biomedical field. To obtain an efficient and stable nanoantioxidant, a novel biosystem was designed by integrating a lipophilic derivative of epigallocatechin-3-gallate (named EGCG-C18) on the surface of poly(lactic-co-glycolic acid) (PLGA). Poly(vinyl alcohol) (PVA) and 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-poly(ethylene glycol) (DSPE-PEG2000) were selected as polymeric and lipidic stabilizers, respectively, and their influence on both physical properties and the antioxidant activity of nanoantioxidant was investigated by a combined in silico and experimental approach. Full-atom molecular dynamics (MD) simulations were carried out to describe the different self-assembly processes of all components and the interactions that guided the EGCG-C18 insertion inside the PLGA matrix. Together with infrared spectroscopy results, the formation of an antioxidant lipid shell on the PLGA surface was clear. Dynamic light scattering and transmission electron microscopy showed that in the presence of DSPE-PEG2000, NPs were smaller than those treated with PVA. In addition, the different stabilizers used strongly influenced the ROS-scavenging ability of nanomaterials and this effect was strictly related to the molecular organization of EGCG-C18. MD showed that the apolar interaction between the alkyl chains of DSPE-PEG2000 and EGCG-C18 oriented the phenolic groups of the polyphenol toward the solvent, providing an ability of NP to scavenge hydroxyl radicals over to free EGCG-C18 and PLGA/PVA NPs. Finally, the ability of nanoantioxidants to protect human dermal fibroblasts from cell death induced by oxidative stress has been tested, revealing the high potential of these novel NPs as polyphenol-based materials.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/325971

FTIR spectroscopy for determination of the raw materials used in wood pellet production
FUEL
Autore/i: Toscano, G.; Maceratesi, V.; Leoni, E.; Stipa, P.; Laudadio, E.; Sabbatini, S.
Classificazione: 1 Contributo su Rivista
Abstract: The research investigates the possibility of distinguishing pellet wood fuels using infrared spectroscopy. It has been applied Attenuated Total Reflectance – Fourier Transform InfraRed (ATR-FTIR) spectroscopy, deemed an analysis technique appropriate for detecting the chemical composition of materials, to determine woody components make up a wood pellet. Considering the large quantities of this solid biofuel on the market, it is necessary to guarantee the safety and traceability aspects of the product, as its origin and source, in addition to the quality parameters indicated by the ISO 17225-2 standards. In this study, a total of 98 samples of the most common wood used for European pellet production, have been selected and collected. This set of samples include also materials coming from the wood industry, as residues of the chemical process. Through clustering analysis Principal component Analysis (PCA) and Hierarchical Cluster Analysis (HCA) techniques applied to spectroscopic analyzer data, it has been seen that ATR-FTIR results provide information on wood pellet typology, hardwood and softwood, and pellet made by woody materials containing high quantity of chemical products like glues.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/297702

Organic enrichment can increase the impact of microplastics on meiofaunal assemblages in tropical beach systems
ENVIRONMENTAL POLLUTION
Autore/i: Corinaldesi, Cinzia; Canensi, Sara; Carugati, Laura; Lo Martire, Marco; Marcellini, Francesca; Nepote, Ettore; Sabbatini, Simona; Danovaro, Roberto
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/315368

New waste-derived TiO2 nanoparticles as a potential photocatalytic additive for lime based indoor finishings
JOURNAL OF CLEANER PRODUCTION
Autore/i: Maqbool, Qaisar; Czerwinska, Natalia; Giosue', Chiara; Sabbatini, Simona; Ruello, Maria Letizia; Tittarelli, Francesca
Classificazione: 1 Contributo su Rivista
Abstract: Production of sustainable nanomaterials (NMs) through the valorization of heterogeneous wastes is of high importance due to escalating environmental sustainability and depletion of natural resources. Following this motive, in this paper, TiO2 nanoparticles (NPs) were green-synthesized (GS) using metal ions reducing potential of secondary metabolites from organic waste (i.e., autumn leaves). Scanning electron microscopy (SEM) and Xray diffraction (XRD) analysis revealed that GS TiO2-NPs as pure anatase for crystalline fraction, with homogenous granulometry ranging between 12 and 26 nm in size, and crystallite size as small as 3.85 (±0.02) nm are achieved. The effect of carbon-content (modifier) on the structural and surface chemistry of tested TiO2-NPs were deeply investigated through Fourier-transform infrared (FTIR) spectroscopy and thermogravimetry analysis (TG/DTG/DTA). The GS TiO2-NPs have shown photocatalytic (NO)x degradation of around 30% and 18% under UV and visible-light respectively, 10% and 70% higher than that of two commercial TiO2-NPs, P-25 and KRONOS-7404, respectively. Based on the leading performance, GS TiO2-NPs were further tested as an additive to an indoor hydraulic-lime based finishing material to augment its photocatalytic properties. Interestingly, GS TiO2-NPs were able to maintain (NO)x photodegradation (≈11%) performance even at an extremely small concentration of <2 wt%. Hence, waste-mediated production of ecofriendly GS TiO2-NPs introduced in this study will help for next-generation photocatalytic indoor finishing materials.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/305843

Transformation of industrial and organic waste into titanium doped activated carbon – cellulose nanocomposite for rapid removal of organic pollutants
JOURNAL OF HAZARDOUS MATERIALS
Autore/i: Maqbool, Qaisar; Barucca, Gianni; Sabbatini, Simona; Parlapiano, Marco; Ruello, Maria Letizia; Tittarelli, Francesca
Classificazione: 1 Contributo su Rivista
Abstract: Production of cost-efficient composite materials with desired physicochemical properties from low-cost waste material is much needed to meet the growing needs of the industrial sector. As a step forward, the current study reports for the first time an effective utilization of industrial metal (inorganic) waste as well as fall leaves (organic waste), to produce three types of nanomaterials at the same time; “Titanium Doped Activated Carbon Nanostructures (Ti-ACNs)”, “Nanocellulose (NCel)”, and combination of both “Titanium Doped Activated Carbon Cellulose Nanocomposite (Ti-AC-Cel-NC)”. X-ray diffraction (XRD), transmission electron microscopy (TEM) and microanalysis (EDXS) measurements reveal that the Ti-ACNs material is formed by Ti-nanostructures, generally poorly crystalized but in some cases forming hexagonal Ti-crystallites of 15 nm, embedded in mutated graphene clouds. Micro- Fourier transform infrared spectroscopy (micro-FTIR) confirms that the chemical structure of NCel with bond vibrations between 1035 to 2917 cm 1 remained preserved during Ti-AC-Cel-NC formation. The prepared materials (Ti-ACNs, Ti-AC-Cel-NC) have demonstrated rapid removal of organic pollutants (Crystal Violet, Methyl Violet) from wastewater through surface adsorption and photocatalysis. In the first 20 min, Ti-ACNs have adsorbed ≈87% of the organic pollutants and further photocatalyzed them up to ≈96%. When Ti-ACNs are combined with NCel, their efficiency is increased of about four times. This performance originates from the adsorption by mutated graphene-like carbon and assisted photocatalysis by Ti nanostructures as well as the good supporting capacity of NCel for the homogenous Ti-ACNs distribution.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/291730

Hyperspectral characterization of the MSTO-211H cell spheroid model: A FPA–FTIR imaging approach
CLINICAL SPECTROSCOPY
Autore/i: Notarstefano, Valentina; Sabbatini, Simona; Sabbatini, Maurizio; Arrais, Aldo; Belloni, Alessia; Pro, Chiara; Vaccari, Lisa; Osella, Domenico; Giorgini, Elisabetta
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/290818

Effects of different pre-treatments on the properties of polyhydroxyalkanoates extracted from sidestreams of a municipal wastewater treatment plant
SCIENCE OF THE TOTAL ENVIRONMENT
Autore/i: Palmieri, S.; Tittarelli, F.; Sabbatini, S.; Cespi, M.; Bonacucina, G.; Eusebi, A. L.; Fatone, F.; Stipa, P.
Classificazione: 1 Contributo su Rivista
Abstract: The paper deals with effects of two different widespread extraction methods (conventional extraction and Soxhlet extraction) and four different pre-treatments (homogenization with pressure and with blades, sonication, and impact with glass spheres) on the extraction yields and properties of polyhydroxyalkanoate (PHA) extracted from biomass coming from an innovative process (short-cut enhanced phosphorus and PHA recovery) applied in a real wastewater treatment plant. The results show that the two different extraction processes affected the crystallization degree and the chemical composition of the polymer. On the other hand, the extractive yield was highly influenced by pre-treatments: homogenization provided a 15% more extractive yield than the others. Homogenization, especially at high pressure, proved to be the best pre-treatment also in terms of the purity, visual appearance (transparency and clearness), thermal stability, and mechanical performances of the obtained PHA films. All the PHA films begin to melt long before their degradation temperature (Td > 200 °C): this allows their use in the fields of extrusion or compression moulding.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/291675

Microplastics in real wastewater treatment schemes: Comparative assessment and relevant inhibition effects on anaerobic processes
CHEMOSPHERE
Autore/i: Pittura, L.; Foglia, A.; Akyol, C.; Cipolletta, G.; Benedetti, M.; Regoli, F.; Eusebi, A. L.; Sabbatini, S.; Tseng, L. Y.; Katsou, E.; Gorbi, S.; Fatone, F.
Classificazione: 1 Contributo su Rivista
Abstract: The occurrence, fate and removal of microplastics (MPs) in a wastewater treatment plant (WWTP) in Central Italy were investigated together with their potential adverse effects on anaerobic processes. In the influent of the WWTP, 3.6 MPs.L−1 were detected that mostly comprised polyester fibers and particles in the shape of films, ranging 0.1–0.5 mm and made of polyethylene and polypropylene (PP). The full-scale conventional activated sludge scheme removed 86% of MPs, with the main reduction in the primary and secondary settling. MPs particles bigger than 1 mm were not detected in the final effluent and some loss of polymers types were observed. In comparison, the pilot-scale upflow granular anaerobic sludge blanket (UASB) + anaerobic membrane bioreactor (AnMBR) configuration achieved 94% MPs removal with the abatement of 87% of fibers and 100% of particles. The results highlighted an accumulation phenomenon of MPs in the sludge and suggested the need to further investigate the effects of MPs on anaerobic processes. Accordingly, PP-MPs at concentrations from 5 PP-MPs.gTS−1 to 50 PP-MPs.gTS−1 were spiked in the pilot-scale UASB reactor that was fed with real municipal wastewater, where up to 58% decrease in methanogenic activity was observed at the exposure of 50 PP-MPs.gTS−1. To the best of our knowledge, the presented results are the first to report of PP-MPs inhibition on anaerobic processes.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/289477

Comparison of two curing protocols during adhesive cementation: can the step luting technique supersede the traditional one?
ODONTOLOGY
Autore/i: Tosco, Vincenzo; Monterubbianesi, Riccardo; Orilisi, Giulia; Sabbatini, Simona; Conti, Carla; Özcan, Mutlu; Putignano, Angelo; Orsini, Giovanna
Classificazione: 1 Contributo su Rivista
Abstract: This study aims to compare the degree of conversion of two different curing protocols used during adhesive cementation. The following resin luting agents were tested: Hri Flow (MF) and pre-heated Hri Micerium (MH); light-cure Nexus Third Generation (NX3L) and dual-cure Nexus Third Generation (NX3D); dual cured RelyX Ultimate (RXU) and light-cure RelyX Veneers (RXL). For each tested material, ten samples were prepared and divided into two groups which had different curing protocols (P1 and P2): in P1, samples were cured for 40 s; in P2, samples were cured for 5 s, and then, after 20 s, cured again for additional 40 s. The degree of conversion (DC) was evaluated both during the first 5 min of the curing phase and after 1, 2, 7, 14 and 28 days (p = 0.05). Different trends were observed in DC values after 5 min by comparing P1 and P2. In both P1 and P2, DC decreased as follows, MH > MF > NX3L > RXL > RXU > NX3D. There were significant differences of DC values among all resin luting agents (p < 0.05) in P1, while no significant differences existed between MH and MF, and NX3L and RXL in P2. At 1, 2, 7, 14 and 28 days the light curing luting agents had a higher DC than the dual luting agents (p < 0.05). P1 and P2 were not statistically different at each time point (p > 0.05). Both P1 and P2 protocols let achieve an acceptable DC after 28 days. The tested P2 can be safely used to lute indirect restorations, simplifying the removal of cement excesses.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/285649

Antioxidant Activity Level, Bioactive Compounds, Colour and Spectroscopic Analysis (UV-Vis and FT-IR) of Flavoured Drinks Made with Wine and Sour Cherries (Prunus cerasus Var. austera)
FOODS
Autore/i: Pisani, M; Astolfi, P.; Sabbatini, S.; Carloni, P.
Classificazione: 1 Contributo su Rivista
Abstract: In recent years, the increase in consumer interest towards simpler and authentic lifestyles has led to an explosive growth in the production and business of typical agri-food products and, among these, of wines and its derived beverages. With the aim of promoting a typical Italian beverage, the so-called “Vino di visciole” or “Visner”, listed in the national table of traditional agrifood products, the antioxidant and colour properties of fifteen samples from different provinces of the Marche region and obtained with different recipes were analysed. The “in vitro” total antioxidant activity (TAA) determined using ABTS assays, total phenolic content (TPC), total flavonoid content (TFC), total anthocyanins content (TAC), and colour (Somers assay) were measured. In addition, a spectroscopic FT-IR and UV-Vis analysis was carried out to analyse samples with multivariate techniques. The results showed that the production area, the recipe, and the type of cherries used to make the alcoholic beverage do not influence the antioxidant properties and the phytochemical contents of the samples. The multivariate treatment of the spectroscopic features (mainly UV-Vis) rather allowed the differentiation of samples with high antioxidant activity using easy and low-cost instrumental techniques that require little time and can be employed in routine analysis.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/291762

Cytotoxic Effects of 5-Azacytidine on Primary Tumour Cells and Cancer Stem Cells from Oral Squamous Cell Carcinoma: An In Vitro FTIRM Analysis
CELLS
Autore/i: Notarstefano, Valentina; Belloni, Alessia; Sabbatini, Simona; Pro, Chiara; Orilisi, Giulia; Monterubbianesi, Riccardo; Tosco, Vincenzo; Byrne, Hugh J.; Vaccari, Lisa; Giorgini, Elisabetta
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/291741

Cellulose Acetate-Graft-Poly (Methyl Methacrylate): A “Graft from” Approach of Nitroxide Mediated Radical Polymerization
JOURNAL OF CHEMICAL ENGINEERING & PROCESS TECHNOLOGY
Autore/i: Vernon, TEBONG MBAH; Celli, A; Cipolletti, R; Sabbatini, S; Stipa, P
Classificazione: 1 Contributo su Rivista
Abstract: Poly (methyl methacrylate) was grafted onto cellulose acetate backbone using a “graft from” of Nitroxide Mediated Radical Polymerization (NMRP). The formation of cellulose acetate-co-poly (methyl methacrylate) using N-tert-butyl- N- (1-diethylphosphono-2,2-dimethylpropyl) (SG1)-nitroxide based macroalkoxyamine system was confirmed by FTIR and NMR analyses. The copolymer exhibited living characteristics as shown by NMR. DSC investigations showed a copolymer with a rich poly (methyl methacrylate) phase around 120°C and a rich cellulose acetate phase at around 175°C.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/276913

How Can Different Polishing Timing Influence Methacrylate and Dimethacrylate Bulk Fill Composites? Evaluation of Chemical and Physical Properties
BIOMED RESEARCH INTERNATIONAL
Autore/i: Monterubbianesi, R.; Tosco, V.; Sabbatini, S.; Orilisi, G.; Conti, C.; Ozcan, M.; Orsini, G.; Putignano, A.
Classificazione: 1 Contributo su Rivista
Abstract: The polishing procedure is commonly performed after direct composite restorations, and little information exists regarding the right timing during which it should be performed on bulk fill composites. This study investigated the effect of polishing timing on the degree of conversion (DC), Vickers microhardness (VMH), and surface morphology of a methacrylate- (MET-) and dimethacrylate- (DMET-) based bulk fill composite, by using FT-NIR, microhardness tester, and SEM. Composite samples were divided as follows: in Group I (immediate), samples were polished immediately after curing (t0); in Group D (delayed), samples were polished after 24 h from curing (t24), whereas the unpolished samples were considered as controls (Group C). The DC and VMH values were evaluated before and after polishing, at t0 and t24. Statistical analysis was performed with a significance level set at p<0.05. At t0, DC increased after polishing in both tested composites (p<0.05), while at t24, Group I and Group D were not different. By considering VMH, in the case of MET, all groups were not different both at t0 and t24. On the other hand, at t0, VMH values of DMET increased after polishing. At t24, DMET Group I and DMET Group D were not different. Qualitative evaluations of scanning electron micrographs showed that the surface morphology of MET presented a more irregular aspect than the DMET one. In summary, since the immediate polishing of MET can improve the DC, without negatively affecting VMH, but showing an irregular surface, it is suggested to wait 24 hours before proceeding with polishing. Otherwise, for DMET, the immediate polishing could definitively be recommended, since it improves both DC and VMH, also producing a regular surface. Therefore, clinicians may always safely polish a restoration performed using DMET-based bulk fill composites in one-chair appointment, avoiding a second appointment.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/277060

Experimental investigation on the durability of a novel lightweight prefabricated reinforced-EPS based construction system
CONSTRUCTION AND BUILDING MATERIALS
Autore/i: D'Orazio, Marco; Stipa, Pierluigi; Sabbatini, Simona; Maracchini, Gianluca
Classificazione: 1 Contributo su Rivista
Abstract: This paper investigates the durability of a low-cost construction system named HOMEDONE developed to realize affordable and also temporary housing solutions. The system is based on the assembly of 3D-reinforced EPS panels externally topped off with a multi-layer rendering system. Similar technologies showed durability issues, especially in hot climates, due to the thermal and hygrometric stresses of the thin finishing layers when coupled to thick EPS panels and exposed to extreme events. For this reason, in this work freeze–thaw and wet/drying-UV aging tests on HOMEDONE panels with different finishing systems have been carried out, monitoring macroscopic, microscopic (ATR-FT-IR analysis) and bond strength variations due to aging. Results have pointed out good mechanical properties of the system and only small color variations of the finishing layer due to UV cycles. Then, HOMEDONE can be considered as a durable option for affordable and temporary housing solutions.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/277420

Investigation of human pancreatic cancer tissues by Fourier Transform Infrared Hyperspectral Imaging
JOURNAL OF BIOPHOTONICS
Autore/i: Notarstefano, Valentina; Sabbatini, Simona; Conti, Carla; Pisani, Michela; Astolfi, Paola; Pro, Chiara; Rubini, Corrado; Vaccari, Lisa; Giorgini, Elisabetta
Classificazione: 1 Contributo su Rivista
Abstract: Fourier-transform infrared hyperspectral imaging (FTIR-HSI) provides hyperspectral images containing both morphological and chemical information. It is widely applied in the biomedical field to detect tumor lesions, even at the early stage, by identifying specific spectral biomarkers. Pancreatic neoplasms present different prognoses and are not always easily classified by conventional analyses. In this study, tissue samples with diagnosis of pancreatic ductal adenocarcinoma and pancreatic neuroendocrine tumor were analyzed by FTIR-HSI and the spectral data compared with those from healthy and dysplastic samples. Multivariate/univariate approaches were complemented to hyperspectral images, and definite spectral markers of the different lesions identified. The malignant lesions were recognizable both from healthy/dysplastic pancreatic tissues (high values of phospholipids and triglycerides with shorter, more branched and less unsaturated alkyl chains) and between each other (different amounts of total lipids, phosphates and carbohydrates). These findings highlight different metabolic pathways characterizing the different samples, well detectable by FTIR-HSI.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/272050

Exploiting fourier transform infrared and Raman microspectroscopies on cancer stem cells from oral squamous cells carcinoma: new evidence of acquired cisplatin chemoresistance
ANALYST
Autore/i: Notarstefano, Valentina; Sabbatini, Simona; Pro, Chiara; Belloni, Alessia; Orilisi, Giulia; Rubini, Corrado; Byrne, Hugh J.; Vaccari, Lisa; Giorgini, Elisabetta
Classificazione: 1 Contributo su Rivista
Abstract: Oral Squamous Cells Carcinoma (OSCC) is characterised by the risk of recurrence and the onset of a refractoriness response to chemotherapy drugs. These phenomena have been recently related to a subpopulation of Cancer Stem Cells (CSCs), which have either an innate or acquired drug resistance, triggered by chemotherapy treatments. In this light, to precisely target chemotherapy regimens, it is essential to improve knowledge on CSCs, with a particular focus on their molecular features. In this work, a subpopulation of CSCs, isolated by tumour sphere formation from primary OSCC cells, were treated with cisplatin for 16, 24 and 48 hours and analysed by infrared absorption and Raman microspectroscopies. CSC spectral data were compared with those obtained in previous work, for primary OSCC cells treated under the same conditions. Routine viability/apoptosis cell-based assays evidenced in CSCs and primary OSCCs, a similar degree of sensitivity to the drug at 24 hours, while a reversion of the conventional monotonic time response exhibited by OSCCs was shown by CSCs at 48 hours. This peculiar time response was also supported by the analysis of IR and Raman data, which pinpointed alterations in the lipid composition and DNA conformation in CSCs. The results obtained suggest that CSCs, although sharing with OSCC cells a similar sensitivity to cisplatin, display the onset of a mechanism of chemoresistance and enrichment of resistant CSCs as a result of drug treatment, shedding new light on the severe issue of refractoriness of some patients to chemotherapy conventionally used for OSCC.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/286410

50 Years of Chemistry in the Engineering Faculty: From Free Radicals to Nanosystems
The First Outstanding 50 Years of “Università Politecnica delle Marche” Research Achievements in Physical Sciences and Engineering
Autore/i: Stipa, Pierluigi; Sabbatini, Simona; Pisani, Michela; Cardellini, Liberato
Classificazione: 2 Contributo in Volume
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/278702

Optical properties of traditional clay tiles for ventilated roofs and implication on roof thermal performance
JOURNAL OF BUILDING PHYSICS
Autore/i: Di Giuseppe, Elisa; Sabbatini, Simona; Cozzolino, Nikita; Stipa, Pierluigi; D’Orazio, Marco
Classificazione: 1 Contributo su Rivista
Abstract: A remarkable advantage of clay tiles roof coverings in hot climates is the realization of a ventilated air layer between them and the roofing underlay that allows a natural and forced convection through the tiles joints and the channel from eaves to ridge, thus cooling the roof materials. However recently, in many countries, regulatory developments on buildings energy efficiency or buildings sustainability certification protocols are increasingly encouraging the use of alternative strategies, with the aim of reducing the urban heat island (UHI) effect and the buildings’ cooling consumptions. Among them, the use of ‘cool’ materials for roof covering. These mandatory or voluntary measures de facto push the construction products market towards specific directions, risking penalizing traditional components such as clay tiles. This article reports the results of experimental and numerical activities carried out in order to extensively characterize the optical properties of clay tile materials and investigate their impact, also coupled with above sheathing ventilation, on the thermal performance of a ventilated roof under warm-temperate climate. In the first phase of the research, the main optical properties of over 30 different clay products have been experimentally characterized in order to get a clear and extensive picture of such properties for the materials spread in the market. In a second phase, starting from the thermal data collected on an experimental real-scale building, a dynamic energy analysis tool was calibrated and used to perform simulations by varying the optical properties of the roof covering thus assessing the impact on the roof temperatures, also in comparison to a clay tiles roof. The results underline that the use of the above sheathing ventilation obtained through clay tiles is an effective strategy to reduce roof temperatures, even if covering materials are not qualified as ‘cool’, thus impacting on both UHI and indoor comfort.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/258693

Anaerobic membrane bioreactor for urban wastewater valorisation: Operative strategies and fertigation reuse
CHEMICAL ENGINEERING TRANSACTIONS
Autore/i: Foglia, A.; Cipolletta, Giulia; Frison, N.; Sabbatini, S.; Gorbi, S.; Eusebi, A. L.; Fatone, F.
Classificazione: 1 Contributo su Rivista
Abstract: In European scenario, the potential source for water supply from treated wastewater is actually estimated in 1,100 Mm3/y (EU-ENV, 2015). Anaerobic processes compared with conventional aerobic ones, cause a net reduction of the operative costs and possible reuse for fertigation purposes. The tested anaerobic pilot (HRT 6 h and T 30°C) is constituted from a UASB reactor (16 L). Increment of influent organic loading rate (OLR) was studied for 1 year from 1 to 2 kgCOD/m3/d by feeding raw wastewater (Period 1), methanol (Period 2) and fermented supernatant from cellulosic sludge (Period 3).The biogas production was assessed equal to 0.13 m3biogas/kgCOD (Period 1), to 0.57 m3biogas/kgCOD (Period 2) and to 0.24 m3biogas/kgCOD (Period 3) with methane percentages constant around 33%. UASB effluent has not the final quality to comply limit values for water reuse and fertigation, especially for microbiological parameters. Further treatments could be necessary to achieve the removals of bacteria, such as E.Coli, recalcitrant organic traces and metals. Therefore, some advanced post treatments have been studied in this paper after UASB treatments, like UV disinfection, UV coupled with H2O2 and GAC adsorption. The innovative solution is coupling UASB reactor with anaerobic submerged sidestream AnMBR (UF hollow fiber membrane with 0.03 μm of nominal pore-size and 0.5 m2 of surface area KOCH, Puron single bundle). Membrane cleaning was carried out using sodium hypochlorite solution (400 ppm) each 45 days to remove organic fouling and to recover the initial permeability of the membrane. The average operative flux at process temperature was equal to 8.8±1.9 L/h/m2 and operating TMP of 44.6±8.5 mbar was detected. The removal of E.Coli was investigated in the effluent from UASB and in the permeate from AnMBR process. At clean membrane conditions, complete removal of bacteria (99±1%) was found. Furthermore, the determination of microplastics distribution was carried out both in the experimental anaerobic pilot and in the conventional full scale aerobic treatment plant. The effluent microplastics were quantified and the removal role of the different operative units was studied. Copyright © 2019, AIDIC Servizi S.r.l.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/268301

Rearing Zebrafish on Black Soldier Fly (Hermetia illucens): Biometric, Histological, Spectroscopic, Biochemical, and Molecular Implications
ZEBRAFISH
Autore/i: Vargas, Arturo; Randazzo, Basilio; Riolo, Paola; Truzzi, Cristina; Gioacchini, Giorgia; Giorgini, Elisabetta; Loreto, Nino; Ruschioni, Sara; Zarantoniello, Matteo; Antonucci, Matteo; Polverini, Sara; Cardinaletti, Gloriana; Sabbatini, Simona; Tulli, Francesca; Olivotto, Ike
Classificazione: 1 Contributo su Rivista
Abstract: A desirable goal of the aquaculture sector is to replace most of fish meal and fish oil with more sustainable, cost-effective, and environmental friendly ingredients ensuring fish health and welfare standards. Due to minimal environmental impact, compared with most conventional feed commodities, insects deserve a growing attention as candidate ingredients for aquafeeds. The present study investigated, for the first time, the possible application of a 100% insect diet in zebrafish larval rearing. Through a multidisciplinary approach, the major biological responses of fish to the new diets were assessed. Results of biometry, fatty acid composition, expression of genes involved in fish growth, stress response, lipid metabolism, chitinolytic activity, gut inflammation, and liver macromolecular composition suggested a possible application of insect larvae for zebrafish larval rearing. However, further studies are necessary to better understand the use of this insect species in the rearing of fish.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/259446

In vitro FTIR microspectroscopy analysis of primary Oral Squamous Carcinoma cells treated with cisplatin and 5-fluorouracil: a new spectroscopic approach for studying drugcell interaction
ANALYST
Autore/i: Giorgini, Elisabetta; Sabbatini, Simona; Rocchetti, Romina; Notarstefano, Valentina; Rubini, Corrado; Conti, Carla; Orilisi, Giulia; Mitri, Elisa; Bedolla, Diana E.; Vaccari, Lisa
Classificazione: 1 Contributo su Rivista
Abstract: In the present study, human primary oral squamous carcinoma cells treated with cisplatin and 5-fluorouracil were analyzed, for the first time, by in vitro FTIR Microspectroscopy (FTIRM), to improve the knowledge on the biochemical pathways activated by these two chemotherapy drugs. To date, most of the studies regarding FTIRM cellular analysis have been executed on fixed cells from immortalized cell lines. FTIRM analysis performed on primary tumor cells under controlled hydrated conditions provides more reliable information on the biochemical processes occurring in in vivo tumor cells. This spectroscopic analysis allows to get on the same sample and at the same time an overview of the composition and structure of the most remarkable cellular components. In vitro FTIRM analysis of primary oral squamous carcinoma cells evidenced a time-dependent drug-specific cellular response, also including apoptosis triggering. Furthermore, the univariate and multivariate analyses of IR data evidenced meaningful spectroscopic differences ascribable to alterations affecting cellular proteins, lipids and nucleic acids. These findings suggest for the two drugs different pathways and extents of cellular damage, not provided by conventional cell-based assays (MTT assay and image-based cytometry).
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/258826

Infrared spectroscopy as a new tool for studying single living cells: Is there a niche?
BIOMEDICAL SPECTROSCOPY AND IMAGING
Autore/i: Sabbatini, Simona; Giorgini, Elisabetta; Conti, Carla; Orilisi, Giulia
Classificazione: 1 Contributo su Rivista
Abstract: FTIR spectroscopy is an analytical technique widely applied for studying the vibrational fingerprint of organic compounds. In recent years, it has been applied to many biomedical fields because of its potential to detect the composition and molecular structure of various biological materials without the need of probe molecules. The coupling of IR spectrometers with visible microscopes has led to perform the imaging analysis of non-homogeneous samples, such as tissues and cells, in which the biochemical and spatial information are close related. In this review, we report the most significant applications of FTIR to the study of cells in different conditions (fixed, dried and living) with the aim to monitor their biochemical modifications, either induced or naturally occurring.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/253280

Fourier Transform Infrared Imaging analysis of dental pulp inflammatory diseases
ORAL DISEASES
Autore/i: Giorgini, Elisabetta; Sabbatini, Simona; Conti, Carla; Rubini, Corrado; R., Rocchetti; M., Fioroni; Memè, L.; G., Orilisi
Classificazione: 1 Contributo su Rivista
Abstract: Objectives: Fourier Transform Infrared microspectroscopy let characterize the macromolecular composition and distribution of tissues and cells, by studying the interaction between infrared radiation and matter. Therefore, we hypothesize to exploit this analytical tool in the analysis of inflamed pulps, to detect the different biochemical features related to various degrees of inflammation. Materials and Methods: IR maps of 13 irreversible and 12 hyperplastic pulpitis, together with 10 normal pulps, were acquired, compared with histological findings and submitted to multivariate (HCA, PCA, SIMCA) and statistical (one-way ANOVA) analysis. The fit of convoluted bands let calculate meaningful band area ratios (means ± s.d., P < 0.05). Results: The infrared imaging analysis pin-pointed higher amounts of water and lower quantities of type I collagen in all inflamed pulps. Specific vibrational markers were defined for irreversible pulpitis (Lipids/Total Biomass, PhII/Total Biomass, CH2/CH3, and Ty/AII) and hyperplastic ones (OH/Total Biomass, Collagen/Total Biomass, and CH3 Collagen/Total Biomass). Conclusion: The study confirmed that FTIR microspectroscopy let discriminate tissues' biological features. The infrared imaging analysis evidenced, in inflamed pulps, alterations in tissues' structure and composition. Changes in lipid metabolism, increasing amounts of tyrosine, and the occurrence of phosphorylative processes were highlighted in irreversible pulpitis, while high amounts of water and low quantities of type I collagen were detected in hyperplastic samples. © 2017 John Wiley & Sons A/S.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/246522

New Amidinoquinoxaline Nitrones as Antioxidants
Conference Proceedings
Autore/i: Gruber, Nadia; Orelli, Liliana R.; Cipolletti, Roberto; Stipa, Pierluigi; Sabbatini, Simona; Mobbili, Giovanna; Minnelli, Cristina
Classificazione: 4 Contributo in Atti di Convegno (Proceeding)
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/249694

Cell death induced in tumoral epithelial cells: SR-IRMS analysis
Advanced Vibrational Spectroscopy for Biomedical Applications
Autore/i: Sabbatini, Simona; Giorgini, Elisabetta; Rubini, Corrado; Rocchetti, Romina; Conti, Carla; Mitri, Elisa; Vaccari Lisa, .
Classificazione: 4 Contributo in Atti di Convegno (Proceeding)
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/249918

STUDY OF ORAL CAVITY LESIONS BY INFRARED SPECTROSCOPY
JOURNAL OF BIOLOGICAL REGULATORS & HOMEOSTATIC AGENTS
Autore/i: Giorgini, Elisabetta; Conti, Carla; Rocchetti, Romina; Rubini, Corrado; Sabbatini, Simona; Librando, Vito; Tosi, Giorgio
Classificazione: 1 Contributo su Rivista
Abstract: Fourier transform infrared (FTIR) microspectroscopy is considered a useful tool in the biomedical field, for analysing in situ and at cellular level, very small areas of tissues and cells, with minimal sample preparation and without the use of stains or probes. This spectroscopic technique has been successfully applied to analyse biological samples from patients affected by tumoral pathologies, with particular attention to oral cavity lesions. In this study, we describe the application of FTIR microspectroscopy to characterize and discriminate the most recurrent benign and malignant diseases of oral cavity compartment. Infrared maps were acquired on tissues affected by the following pathologies: squamous cell carcinoma, adenoid cystic carcinoma, polymorphous low-grade adenocarcinoma, squamous dysplasia, keratocystic odontogenic tumor, radicular cyst, residual cyst, unicystic ameloblastoma, and ameloblastic fibroma, together with healthy tissue samples (used as control group). The epithelial and connective components of all samples were distinguished and submitted to multivariate analysis. The results were in agreement with histological suggestions.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/246013

Study of the mechanisms induced by chemotherapy drugs on tumoral epithelial cells.
XV Workshop su Pharmaco BioMetallics
Autore/i: Sabbatini, Simona; Rocchetti, Romina; Vaccari, Lisa; Mitri, Elisa; Conti, Carla; Ferroni, Eugenia; Giorgini, Elisabetta
Classificazione: 4 Contributo in Atti di Convegno (Proceeding)
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/249919

Thermal stress effects in tumoral epithelial cells: SR-IRMS analysis
FT‐IR Spectroscopy in Microbiological and Medical Diagnostics
Autore/i: Sabbatini, Simona; Giorgini, Elisabetta; Conti, Carla
Classificazione: 4 Contributo in Atti di Convegno (Proceeding)
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/249916

VIBRATIONAL MAPPING OF SINONASAL LESIONS BY FOURIER TRANSFORM INFRARED IMAGING SPECTROSCOPY
JOURNAL OF BIOMEDICAL OPTICS
Autore/i: Giorgini, Elisabetta; Sabbatini, Simona; Conti, Carla; Rubini, Corrado; Rocchetti, Romina; Re, Massimo; Vaccari, Lisa; Mitri, Elisa; Librando, Vito
Classificazione: 1 Contributo su Rivista
Abstract: Fourier transform infrared imaging (FTIRI) is a powerful tool for analyzing biochemical changes in tumoral tissues. The head and neck region is characterized by a great variety of lesions, with different degrees of malignancy, which are often difficult to diagnose. Schneiderian papillomas are sinonasal benign neoplasms arising from the Schneiderian mucosa; they can evolve into malignant tumoral lesions (squamous cell carcinoma). In addition, they can sometimes be confused with the more common inflammatory polyps. Therefore, an early and definitive diagnosis of this pathology is mandatory. Progressing in our research on the study of oral cavity lesions, 15 sections consisting of inflammatory sinonasal polyps, benign Schneiderian papillomas, and sinonasal undifferentiated carcinomas were analyzed using FTIRI. To allow a rigorous description of these pathologies and to gain objective diagnosis, the epithelial layer and the adjacent connective tissue of each section were separately investigated by following a multivariate analysis approach. According to the nature of the lesion, interesting modifications were detected in the average spectra of the different tissue components, above all in the lipid and protein patterns. Specific band-area ratios acting as spectral markers of the different pathologies were also highlighted. © 2015 Society of Photo-Optical Instrumentation Engineers (SPIE) [DOI: 10.1117/1.JBO.20.12.125003]
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/230132

FTIR microspectroscopic characterization of Spitz nevi
SPECTROCHIMICA ACTA. PART A, MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
Autore/i: Giorgini, Elisabetta; Tosi, Giorgio; Conti, Carla; Staibano, Stefania; Ilardi, Gennaro; Sabbatini, Simona
Classificazione: 1 Contributo su Rivista
Abstract: In the last 10 years, few efforts have been carried out to apply vibrational spectroscopy in the study of dermal pathologies in order to characterize the most relevant spectral markers for distinguishing benign from cancerous lesions. Spitz nevi are a special group of benign melanocytic lesions, characterized by spindled and/or epithelioid nevomelanocytes, with peculiar clinical, dermoscopic and histopathological features. The ‘‘atypical forms’’ of Spitz nevi are among the commonest problems of differential diagnosis with the so-called ‘‘spitzoid melanomas’’. The clinical and histological criteria for discriminating these two entities are very subtle and often still quite subjective, and, in a significant percentage of cases, can lead to diagnostic pitfalls and inadequate therapies. Therefore, it is noteworthy to outline that the diagnosis of melanocytic lesions still represents a challenging problem and a continue matter of discussion. We exploited FTIR microspectroscopy to study the different kinds of spitzoid melanocytes, in order to define the most relevant spectral markers of each specimen and to achieve objective information on ‘‘borderline’’ histologically atypical lesions. In particular, the spectroscopic investigation was carried out on melanocytes deriving from normal skin (as a normal control), malignant melanoma and Spitz nevi. The presence of the characteristic bands of melanin was investigated, too.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/228245

FTIR Characterization of nasal polyps lesions
SPEC 2014 Shedding New Light on Disease
Autore/i: Sabbatini, Simona; Tosi, Giorgio; Conti, Carla; Vaccari, Lisa; Rubini, Corrado; Re, M.; Giorgini, Elisabetta
Classificazione: 4 Contributo in Atti di Convegno (Proceeding)
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/249917

VIBRATIONAL CHARACTERIZATION OF FEMALE GAMETES: A COMPARATIVE STUDY
ANALYST
Autore/i: Giorgini, Elisabetta; Gioacchini, Giorgia; Sabbatini, Simona; Conti, Carla; L., Vaccari; A., Borini; Carnevali, Oliana; Tosi, Giorgio
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/184105

A new approach to evaluate aging effects on human oocytes: Fourier transform infrared (FTIR) imaging spectroscopy study.
FERTILITY AND STERILITY
Autore/i: Gioacchini, Giorgia; Giorgini, Elisabetta; Vaccari, L; Ferraris, P; Sabbatini, Simona; Bianchi, V; Borini, A; Carnevali, Oliana
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/128301

Infrared microspectroscopy of Oral Squamous Cell Carcinoma:Spectral signatures of cancer grading
VIBRATIONAL SPECTROSCOPY
Autore/i: Sabbatini, Simona; Conti, Carla; Rubini, Corrado; Vito, Librando; Tosi, Giorgio; Giorgini, Elisabetta
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/115462

Insights on diagnosis of oral cavity pathologies by infrared spectroscopy: A review
JOURNAL OF MOLECULAR STRUCTURE
Autore/i: Giorgini, Elisabetta; Balercia, P; Conti, Carla; Ferraris, P; Sabbatini, Simona; Rubini, Corrado; Tosi, Giorgio
Classificazione: 1 Contributo su Rivista
Abstract: Fourier-Transform Infrared microspectroscopy, a largely used spectroscopic technique in basic and industrial researches, offers the possibility to analyze the vibrational features of molecular groups within a variety of environments. In the bioclinical field, and, in particular, in the study of cells, tissues and biofluids, it could be considered a supporting objective technique able to characterize the biochemical processes involved in relevant pathologies, such as tumoral diseases, highlighting specific spectral markers associable with the principal biocomponents (proteins, lipids and carbohydrates). In this article, we review the applications of infrared spectroscopy to the study of tumoral diseases of oral cavity compartments with the aim to improve understanding of biological processes involved during the onset of these lesions and to afford to an early diagnosis. Spectral studies on mouth, salivary glands and oral cystic lesions, objectively discriminate normal from dysplastic and cancer states characterizing also the grading.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/124462

The role of melatonin on Zebrafish follicle development: an FT-IR imaging approach
VIBRATIONAL SPECTROSCOPY
Autore/i: Giorgini, Elisabetta; Gioacchini, Giorgia; Conti, Carla; P., Ferraris; Sabbatini, Simona; Tosi, Giorgio; C. C., Piccinetti; L., Vaccari; Carnevali, Oliana
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/82205

Electrospun Nanostructured Fibers of Collagen-Biomimetic Apatite on Titanium Alloy
BIOINORGANIC CHEMISTRY AND APPLICATIONS
Autore/i: Iafisco, M.; Foltran, I.; Sabbatini, Simona; Tosi, Giorgio; Roveri, N.
Classificazione: 1 Contributo su Rivista
Abstract: Titanium and its alloys are currently the mainly used materials to manufacture orthopaedic implants due to their excellent mechanical properties and corrosion resistance. Although these materials are bioinert, the improvement of biological properties (e.g., bone implant contact) can be obtained by the application of a material that mimics the bone extracellular matrix. To this aim, this work describes a new method to produce nanostructured collagen-apatite composites on titanium alloy substrate, by combining electrospinning and biomimetic mineralization. The characterization results showed that the obtained mineralized scaffolds have morphological, structural, and chemical compositional features similar to natural bone extracellular matrix. Finally, the topographic distribution of the chemical composition in the mineralized matrix evaluated by Fourier Transform Infrared microspectroscopy demonstrated that the apatite nanocrystals cover the collagen fibers assembled by the electrospinning.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/87360

Vibrational spectroscopy as a supporting technique in clinical diagnosis and prognosis of atherosclerotic carotid plaques: a review
ANALYTICAL AND QUANTITATIVE CYTOLOGY AND HISTOLOGY
Autore/i: Tosi, Giorgio; Giorgini, Elisabetta; Rubini, Corrado; Sabbatini, Simona; V., Librando; F., Alò
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/81148

Microimaging FT-IR of head and neck tumors.V. Odontogenic cystic lesions
VIBRATIONAL SPECTROSCOPY
Autore/i: Tosi, Giorgio; Balercia, P.; Conti, Carla; Ferraris, P.; Giorgini, Elisabetta; Lo Muzio, L.; Sabbatini, Simona; Stramazzotti, Daniela; Rubini, Corrado
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/57644

FT-IR microscopic analysis on human dental pulp stem cells
VIBRATIONAL SPECTROSCOPY
Autore/i: Giorgini, Elisabetta; Conti, Carla; Ferraris, P; Sabbatini, Simona; Tosi, Giorgio; Centonze, M; Grano, M; Mori, G.
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/57643

FTIR microspectroscopy of melanocytic skin lesions: a preliminary study.
ANALYST
Autore/i: Tosi, Giorgio; Conti, Carla; Giorgini, Elisabetta; Ferraris, P; Garavaglia, Mg; Sabbatini, Simona; Staibano, S; Rubini, Corrado
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/55089

Oocytes maturation induction by Lactobacillus rhamnosus in Danio rerio: in vivo and in vitro studies
HUMAN REPRODUCTION
Autore/i: Gioacchini, Giorgia; Bizzaro, Davide; Giorgini, Elisabetta; Ferraris, P.; Sabbatini, Simona; Carnevali, Oliana
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/54494

Effects of Lactobacillus rhamnosus on zebrafish oocyte maturation: an FTIR imaging and biochemical analysis
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
Autore/i: Giorgini, Elisabetta; Conti, Carla; Ferraris, P.; Sabbatini, Simona; Tosi, Giorgio; Rubini, Corrado; Vaccari, L.; Gioacchini, Giorgia; Carnevali, Oliana
Classificazione: 1 Contributo su Rivista
Abstract: The aim of this study was to verify the effects of probiotic Lactobacillus rhamnosus on zebrafish oocyte maturation using FPA (focal plane array) FTIR imaging together with specific biochemical assays (SDS–PAGE, real-time PCR and enzymatic assay). Oocyte growth is prevalently due to a vitellogenic process which consists of the hepatic synthesis of vitellogenin and its selective uptake during maturation. The administration of L. rhamnosus IMC 501 for 10 days induced chemical changes to oocyte composition, promoting the maturation process. Some interesting biochemical features, linked to protein secondary structure (amide I band) and to phospholipidic and glucidic patterns, were detailed by vibrational analysis. The spectroscopic results were supported by the early increase of the lysosomal enzyme involved in the final oocyte maturation, the cathepsin L. This enzyme increases during follicle maturation, with the highest levels in class IV oocytes. In treated females, class III oocytes showed higher cathepsin L gene expression and enzymatic activity, with levels comparable to class IV oocytes isolated from controls; this can be related to the proteolytic cleavage of the higher molecular mass yolk protein components, as evidenced by SDS–PAGE.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/54459

Micro-FTIR imaging spectroscopy of calcified atheromatous carotid plaques. IV
JOURNAL OF MOLECULAR STRUCTURE
Autore/i: Alò, F; Conti, Carla; Ferraris, P; Giorgini, Elisabetta; Rubini, Corrado; Sabbatini, Simona; Tosi, Giorgio
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/52963

Microimaging FTIR of head and neck tumors. IV.
MICROSCOPY RESEARCH AND TECHNIQUE
Autore/i: Conti, Carla; Ferraris, P.; Garavaglia, M.; Giorgini, Elisabetta; Rubini, Corrado; Sabbatini, Simona; Tosi, Giorgio
Classificazione: 1 Contributo su Rivista
Abstract: On continuing our studies on head and neck neoplasia, specimens from salivary gland tumors have been explored by using infrared microimaging spectroscopy to discern healthy from neoplastic tissues. Samples with Warthin tumor, epithelial displasia, marginal B-cell lymphoma, low-grade adenocarcinoma, and adenoid cystic carcinoma pathologies have been investigated by using conventional light sources. Changes were monitored at the molecular level, probing spectral markers such as Amide I and II, phosphate, nucleic acids, and carbohydrates vibrational modes. In all cases, supervised and unsupervised spectral analyses resulted in satisfactory agreement with histopathological findings.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/52344

FT-IR Microspectroscopy on molecular building of Zebrafish oocytes
JOURNAL OF MOLECULAR STRUCTURE
Autore/i: Carnevali, Oliana; Conti, Carla; Ferraris, P; Garavaglia, Mg; Gioacchini, Giorgia; Giorgini, Elisabetta; Rubini, Corrado; Sabbatini, Simona; Tosi, Giorgio
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/51257

Microimaging FT-IR spectroscopy on pathological breast tissues
VIBRATIONAL SPECTROSCOPY
Autore/i: Anastassopoulou, J; Boukaki, E; Conti, Carla; Ferraris, P; Giorgini, Elisabetta; Rubini, Corrado; Sabbatini, Simona; Theophanides, T; Tosi, Giorgio
Classificazione: 1 Contributo su Rivista
Abstract: Microimaging Fourier transform infrared spectroscopy is able to monitor differentiation between normal and malignant tissues. All the specimens, previously submitted to histological analysis, displayed abnormal spectra compared with the corresponding normal tissues with changes in many diagnostic bands like those arising from phosphate, C–O and CH stretching vibrational modes. The comparison between cancer (K) and connective (C) spectra evidenced the following differences: in the vCH region 3000–2800 cm1 no hypomethylation effect was evident in K; the convolution of the bands of connective indicated an expected higher membrane fluidity; in the neoplastic zone, Amide I and II modes showed convoluted bands withmaxima at 1651 and 1547 cm1, respectively, indicating ana-helix conformation of proteins due to changes in the secondary structure proteins upon carcinogenesis. Other signature bands, such as the deformation O–P–O phosphate band at 965 cm1, suggested DNA conformational changes in solid cancer, infiltrating cancer and neoplasia in the region 1350–800 cm1. These characteristic bands have been monitored as a function of the degree of cancer progression. Chemometric methods, such as principal component analysis (PCA) and hierarchical clustering analysis (HCA) have been used in order to distinguish spectra of neoplastic and normal zones.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/52396

Micro-FTIR imaging spectroscopy of calcified atheromatous carotid plaques
JOURNAL OF MOLECULAR STRUCTURE
Autore/i: Alo', Francesco Paolo; Carla, Conti; Paolo, Ferraris; Giorgini, Elisabetta; Corrado, Rubini; Sabbatini, Simona; Giorgio, Tosi
Classificazione: 1 Contributo su Rivista
Abstract: Micro-imaging infrared spectroscopy has been performed on atheromatous plaques in order to localize and characterize substances responsible for the cytotoxic effects that prevent macrophages clearance of lipidic and calcified materials. In plaques with different graded atherosclerotic lesions, infrared determinations allowed to visualize gruel and ceroid toxic components and variously calcified zones. Compare correlations let to visualize the progression of the lesion on going from the lumen to the outer media of the plaque.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/66746

MICROIMAGING FT-IR OF HEAD AND NECK TUMOURS THE CASE OF SALIVARY GLAND PATHOLOGIES
ANTICANCER RESEARCH
Autore/i: Conti, C.; Ferraris, P.; Giorgini, E.; Rubini, C.; Sabbatini, S.; Tosi, G.
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/83056

Electrochemically assisted deposition of biomimetic hydroxyapatite-collagen coatings on titanium plate.
INORGANICA CHIMICA ACTA
Autore/i: Manara, S; Paolucci, F; Palazzo, B; Marcaccio, M; Foresti, E; Tosi, Giorgio; Sabbatini, Simona; Sabatino, P; Altankov, P; Roveri, N.
Editore: Elsevier BV:PO Box 211, 1000 AE Amsterdam Netherlands:011 31 20 4853757, 011 31 20 4853642, 011 31 20 4853641, EMAIL: nlinfo-f@elsevier.nl, INTERNET: http://www.elsevier.nl, Fax: 011 31 20 4853598 Editore precedente:Prof Ugo Croatto Luogo pubbl. Padova Ultimo luogo pubblicazione: Lausanne.
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/30097

FT-IR microimaging spectroscopy: A comparison between healthy and neoplastic human colon tissues
JOURNAL OF MOLECULAR STRUCTURE
Autore/i: Conti, Carla; Ferraris, P; Giorgini, Elisabetta; Rubini, Corrado; Sabbatini, Simona; Tosi, Giorgio; Anastassopoulou, J; Arapantoni, P; Boukaki, E; Konstadoudakis, S; Theophanides, T; Valavanis, C.
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/51500

MICRO-FT-IR SPECTROSCOPIC STUDIES OF BREAST TISSUES
BRILLIANT LIGHT IN LIFE AND MATERIAL SCIENCES
Autore/i: J., Anastassopoulou; P., Arapantoni; E., Boutaki; S., Konstadoukakis; Valavanis, T. T. H. E. O. P. H. A. N. I. D. E. S. C.; Conti, Carla; P., Ferraris; Giorgini, Elisabetta; Sabbatini, Simona; Tosi, Giorgio
Editore: SPRINGER
Classificazione: 2 Contributo in Volume
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/51615

Micro-FT-IR Spectroscopic studies of breast tissues
NATO Security through Science Series
Autore/i: Anastassopoulou, J.; Arapantoni, P.; Boukaki, E.; Konstadoudakis, S.; Theophanides, T.; Valavanis, C.; Conti, Carla; Ferraris, P.; Giorgini, G.; Sabbatini, Simona; Tosi, G.
Classificazione: 4 Contributo in Atti di Convegno (Proceeding)
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/87395

Analysis of breast normal and tumor tissues by using microimaging fourier transform infrared (FT-IR) spectroscopy
VIRCHOWS ARCHIV
Autore/i: Valavanis, C.; Boukaki, E.; Konstadoudakis, S.; Arapantoni-Dadioti, P.; Anastasopoulou, J.; Theophanides, T.; Conti, C.; Ferraris, P.; Giorgini, E; Rubini, C.; Sabbatini, S.; Tosi, G.
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/83060

Preliminary study of infiltrative colon cancer by using microimaging Fourier Transform infrared (FT-IR) spectroscopy
VIRCHOWS ARCHIV
Autore/i: Valavanis, C.; Konstadoudakis, S.; Boukaki, E.; Arapantoni-Dadioti, P.; Anastasopoulou, J.; Theophanides, T.; Conti, C.; Ferraris, P.; Giorgini, E.; Rubini, C.; Sabbatini, S.; Tosi, G.
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/83058

Microimaging FT-IR of oral cavity tumours. Part III: Cells, inoculated tissues and human tissues
JOURNAL OF MOLECULAR STRUCTURE
Autore/i: Conti, Carla; Ferraris, P; Giorgini, Elisabetta; Pieramici, T; Possati, L; Rocchetti, R; Rubini, Corrado; Sabbatini, Simona; Tosi, Giorgio; Mariggio', Ma; LO MUZIO, L.
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/52786

FPA micro spectral imaging of non-Hodgkin lymphomas
JOURNAL OF MOLECULAR STRUCTURE
Autore/i: Burattini, E; MALVEZZI CAMPEGGI, F; Chilosi, M; Conti, Carla; Ferraris, P; Monti, F; Sabbatini, Simona; Tosi, Giorgio; Zamo', A.
Editore: Tokyo ; Oxford ; New York ; London ; Amsterdam : Elsevier
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/30098

Characterization of Fluoro-edenite by Raman and IR spectroscopy
MINERALOGICAL MAGAZINE
Autore/i: Cairo, S; Gastaldi, D; Gianfagna, A; Rinaudo, C; Sabbatini, Simona; MAZZIOTTI TAGLIANI, S; Tosi, Giorgio
Editore: Mineralogical Society of Great Britain:41 Queen's Gate, Kensington London SW7 5HR United Kingdom:011 44 2 0 75847516, EMAIL: info@minersoc.org, INTERNET: http://www.minersoc.org, Fax: 011 44 20 78238021
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/29133

Resin-based dentin restorative materials under accelerated ageing: bio-functional behavior .
INTERNATIONAL JOURNAL OF ARTIFICIAL ORGANS
Autore/i: MATTIOLI BELMONTE CIMA, Monica; Natali, Desire'; Tosi, Giorgio; Torricelli, P; Totaro, I; Zizzi, Antonio; Fini, M; Sabbatini, Simona; Giavaresi, G; Biagini, G.
Classificazione: 1 Contributo su Rivista
Abstract: The aim of the present study was the evaluation of the effect of different polishing and finishing procedures on Filtek Z250 FZ ESPE restorative material. Particularly, the consequence of artificial aging (UV-irradiation) on this resin-based dental material was investigated determining also its outcome on cell behavior. Methods: 96 specimens of restorative material were prepared using a light emitting diode curing unit and randomly divided into four finishing and polishing groups: (I) No treatment (FZ); (II) Identoflex rubbers (ID); (III) Enhance System (EN) and (IV) Sof-Lex Pop-on XT discs (SF). The surface morphology of native and artificially aged materials was assessed with Atomic Force Microscopy (AFM) and Scanning Electron Microscopy (SEM). FTIR and biological (biocompatibility and bacterial adhesion) analyses were also performed. Results: Among all, the ID procedure represented an acceptable compromise for efficiency of polymerization and biocompatibility both before and after artificial ageing. SF and EN techniques showed better interactions with the biological environment. Conclusion: UV artificial ageing of the tested specimens has shown an acceleration of the surface degrading processes, favoring a possible decrease in the mechanical properties and the release of toxic free radicals. Finishing and polishing procedure seemed to affect the photodegrading pathways, even though no differences among the techniques were observed. As the cytotoxicity of materials undergoing accelerated aging is relevant, further improvement of dental restorative materials are required to limit the long-term biological damage.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/37544

Multiple Component Approaches to C-Glycosyl -Amino Acids by Complementary One-pot Mannich-Type and Reformatsky-Type Reactions.
CHEMISTRY-A EUROPEAN JOURNAL
Autore/i: Dondoni, A.; Massi, A.; Sabbatini, Simona
Classificazione: 1 Contributo su Rivista
Abstract: The development of new methods for the preparation of C-glycosyl b-amino acid libraries with chemical and stereochemical diversity levels was investigated and the results are described herein. Two complementary one-pot three-component Mannichtype and Reformatsky-type synthetic strategies have been developed for the construction of chiral 3-amino propanoate fragments (eventually bis-substituted at C-2) directly linked to the anomeric carbon of pyranose and furanose residues. Both methods involved as the initial step the coupling of a sugar aldehyde to p-methoxybenzylamine but differed in the nucleophile (a d2 synthon equivalent) which was successively added: a ketene silyl acetal (Mannich route) or a bromozinc enolate (Reformatsky route). Individual C-glycosyl b-amino esters were isolated as single 3R diastereoisomers in fair to excellent yield (60–90%) and their structure assigned by NMR spectroscopy (Riguera protocol) supported by X-ray crystallography. A tentative explanation of the observed stereochemical outcome based on transitionstate models is provided. A preliminary study on the synthesis of a,a-difluoro C-glycosyl b-amino acids via a more traditional Reformatsky route is also reported
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/87403

Three-component Staudinger-type Stereoselective Synthesis of C-Glycosyl β-Lactams and Their Use as Precursors for C-Glycosyl Isoserines and Dipeptides. A Polymer-assisted Solution-Phase Approach.
ADVANCED SYNTHESIS & CATALYSIS
Autore/i: Dondoni, A.; Massi, A.; Sabbatini, Simona; Bertolasi, V.
Classificazione: 1 Contributo su Rivista
Abstract: A collection of 4-(C-galactosyl)- and 4-(Cribosyl)- b-lactams featuring different substituents at C-3 and N-1 was prepared by combining in a onepot procedure a formyl C-glycoside, a primary amine, and a substituted acetyl chloride in the presence of base (Staudinger-type reaction).Sulfonyl chloride and aminomethylated resins were used in sequence to remove excess of components and by-products. Two pure C-glycosyl-b-lactams were effectively transformed into C-glycosyl-N-Boc-b-amino-a-hydroxy esters (C-glycosyl isoserines) and a C-ribosyl dipeptide via base-promoted heterocycle ring opening by methanol and l-phenylalanine methyl ester, respectively.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/87405

Synthesis of C-glycosyl β-amino acids by asymmetric Mannich-type three-component reactions.
TETRAHEDRON LETTERS
Autore/i: Dondoni, A.; Massi, A.; Sabbatini, Simona; Bertolasi, V.
Classificazione: 1 Contributo su Rivista
Abstract: C-Galactosyl and C-ribosyl b-amino acids were prepared by one-pot InCl3-catalyzed Mannich-type three-component condensation (3CC) by combining the corresponding formyl C-glycoside, p-methoxybenzyl amine, and a ketene silyl acetal. In each case the reaction was highly stereoselective and afforded only one single product in good to excellent yields.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/87406

Model studies toward the Synthesis of Dihydropyrimidinyl and Pyridyl α-Amino Acids via Three-component Biginelli and Hantzsch Cyclocondensations.
JOURNAL OF ORGANIC CHEMISTRY
Autore/i: Dondoni, A.; Massi, A.; Minghini, E.; Sabbatini, Simona; Bertolasi, V.
Classificazione: 1 Contributo su Rivista
Abstract: A novel and versatile strategy for the synthesis of heterocyclic R-amino acids has been described. The use of components (aldehyde or â-ketoester) bearing a masked glycinyl moiety in Biginelli and Hantzsch cyclocondensations allowed access to the 4-dihydropyrimidinyl-R-glycines, 4-dihydropyrimidinyl- R-alanines, 4-pyridyl-R-alanines, and 2-pyridyl-R-alanines classes. Dihydropyrimidinylamino acids were obtained as a mixture of diastereoisomers due to the formation of the stereocenter at C4 of the dihydropyrimidinone ring. Individual stereoisomers were isolated as pure compounds and their structures were assigned with the aid of X-ray crystallography and chiroptical properties. The enantiomeric purity of a representative selection of the above amino acids was greater than 96% as verified by derivatization to the corresponding Mosher’s amides and subsequent 1H and 19F NMR spectroscopy. Incorporation of the 4-pyridyl-R-alanine derivative into a peptide chain is also described.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/87407

Improved synthesis and preparative scale resolution of racemic monastrol.
TETRAHEDRON LETTERS
Autore/i: Dondoni, A.; Massi, A.; Sabbatini, Simona
Classificazione: 1 Contributo su Rivista
Abstract: The Yb(OTf)3 catalyzed Biginelli cyclocondensation reaction of 3-hydroxybenzaldehyde, ethyl acetoacetate and thiourea afforded the corresponding dihydropyrimidine-2-thione, called monastrol, in 95% isolated yield. The chiral resolution of racemic monastrol, a mitosis blocker by kinesin Eg5 inhibition, was carried out on a preparative scale (ca. 100 mg) through diastereomeric N-3 ribofuranosyl amides.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/87408

Three-Component Biginelli Cyclocondensation Reaction Using C-Glycosylated Substrates. Preparation of a Collection of Dihydropyrimidinone Glycoconjugates and the Synthesis of C-Glycosylated Monastrol Analogues.
JOURNAL OF ORGANIC CHEMISTRY
Autore/i: Dondoni, A.; Massi, A.; Sabbatini, Simona; Bertolasi, V.
Classificazione: 1 Contributo su Rivista
Abstract: The aldehyde-ketoester-urea cyclocondensation reaction has been revisited using C-glycosylated reagents with the aim of exploring a potential entry to a library of dihydropyrimidinone glycoconjugates. A collection of 13 mono- and bis-C-glycosylated dihydropyrimidinones has been prepared by a parallel synthesis approach using the three-component promoter CuCl/AcOH/BF3â Et2O. The sugar residues have been installed at either N1, C4, or C6 in the monoglycosylated derivatives and at both the C4 and C6 in the bisglycosylated products. The mono- and bisglycosylated products at C4 and C6 were obtained as mixtures of diastereoisomers with good to excellent selectivities due to the asymmetric induction by the sugar residue in the formation of the C4 stereocenter of the dihydropyrimidinone ring. Individual stereoisomers were isolated as pure compounds and their structures assigned with the aid of X-ray crystallography and chiroptical properties. As a demonstration of this new concept in the Biginelli reaction, the synthesis of two C4 epimer monastrol analogues bearing the ribofuranosyl moiety at C6 has been described.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/87609

Toward the synthesis of C-glycosylated dihydropyrimidine libraries via the three-component Biginelli reaction. A novel approach to artificial nucleosides.
TETRAHEDRON LETTERS
Autore/i: Dondoni, A.; Massi, A.; Sabbatini, Simona
Classificazione: 1 Contributo su Rivista
Abstract: The Lewis acid catalyzed (BF3·Et2O, CuCl, AcOH) one-pot three-component cyclocondensation of urea with C-glycosylated aldehydes and -keto esters (Biginelli reaction) in a combinatorial manner afforded three different series of 3,4-dihydropyrimidin- 2(1H)-ones bearing C-glycosyl moieties at C4, C6, and at both C4 and C6.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/87409