82 pubblicazioni classificate nel seguente modo:

Patient and Community Health Global Burden in a World with more Celiac Disease
GASTROENTEROLOGY
Autore/i: Gatti, Simona; Rubio-Tapia, Alberto; Makharia, Govind; Catassi, Carlo
Classificazione: 1 Contributo su Rivista
Abstract: : Celiac disease is one of the commonest life-long disorders worldwide, with a prevalence mostly ranging between 0.7 and 2.9 % in the general population and a higher frequency in females and in well-defined at-risk groups, such as relatives of affected individuals and patients with autoimmune co-morbidities. Increasing clinical detection is facilitated by improving awareness, implementation of a case-finding approach, and serology availability for screening at risk patients, among other factors. Nevertheless, due to huge clinical variability many CeD cases still escape diagnosis in most countries, unless actively searched by proactive policies. The burden of celiac disease is increasing as well as the need for better longitudinal care. Pediatric screening of the general population could represent the road ahead for an efficient intervention of secondary prevention aimed to reduce the social and health burden of celiac disease. This review analyses the epidemiology of celiac disease continent by continent, discusses current strategies to improve the detection of celiac disease, and highlights challenges related to the burden of celiac disease globally.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/327711

Major Abdominal Surgery for Pediatric Crohn’s Disease in the Anti-TNF Era: 10-Year Analysis of Data From the IBD Registry of Italian Society of Pediatric Gastroenterology, Hepatology, and Nutrition
INFLAMMATORY BOWEL DISEASES
Autore/i: Alvisi, Patrizia; Faraci, Simona; Scarallo, Luca; Congiu, Marco; Bramuzzo, Matteo; Illiceto, Maria Teresa; Arrigo, Serena; Romano, Claudio; Zuin, Giovanna; Miele, Erasmo; Gatti, Simona; Aloi, Marina; Renzo, Sara; Caldaro, Tamara; Labriola, Flavio; De Angelis, Paola; Lionetti, Paolo
Classificazione: 1 Contributo su Rivista
Abstract: Background The natural history of Crohn's disease (CD) can result in complications requiring surgery. Pediatric data are scarce about major abdominal surgery. The IBD Registry from the Italian Society of Pediatric Gastroenterology, Hepatology, and Nutrition has been active since 2008 and collects data from major pediatric IBD centers in Italy. The aim of the present report was to explore the prevalence of major abdominal surgery among children affected by CD in an era when antitumor necrosis factor (anti-TNF-alpha) agents were already used so that we might appraise the incidence of surgical-related complications and identify the factors associated with postoperative disease recurrence.Methods We retrospectively analyzed data from patients enrolled in the registry from January 2009 to December 2018. Patients with monogenic IBD and patients undergoing surgery for perianal disease were excluded.Results In total, 135 of 1245 patients were identified. We report the prevalence of major abdominal surgery of 10.8%. Pediatric surgeons performed the procedure in 54.1% of cases, and a laparoscopic approach was used in 47.4% of surgical procedures. Seventeen patients (12.6%) experienced a total of 21 early postoperative complications, none of which was severe. A laparoscopic approach was the only factor negatively associated with the occurrence of postoperative complications (odds ratio, 0.22; 95% confidence interval, 0.06-0.8; P = .02). Fifty-four (40%) patients experienced postoperative endoscopic recurrence, and 33 (24.4%) of them experienced postoperative clinical recurrence. The postoperative treatment with anti-TNF-alpha drugs was significantly associated with a reduced risk of endoscopic recurrence (odds ratio, 0.19; 95% confidence interval, 0.05-0.79; P = .02).Conclusion In our cohort, the overall prevalence of major abdominal surgery was low, as well as the rate of surgical-related complications. Postoperative anti-TNF-alpha therapy seems be protective against endoscopic recurrence.Data from the IBD SIGENP registry show that the prevalence of major abdominal surgery is 10.8%, with a relatively low occurrence of short-term postoperative complications. The administration of anti-TNF-alpha drugs after surgery seems to effectively prevent postoperative endoscopic recurrence of disease.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/327713

Performance assessment of a new G12/A1 antibody-based rapid ELISA using commercially available and gluten-spiked food samples
EUROPEAN JOURNAL OF GASTROENTEROLOGY & HEPATOLOGY
Autore/i: Verma, Anil K; Monachesi, Chiara; Catassi, Giulia N; Franceschini, Elisa; Gatti, Simona; Lionetti, Elena; Catassi, Carlo
Classificazione: 1 Contributo su Rivista
Abstract: Objective: Food products with <20 mg/kg gluten can be labeled 'gluten-free' according to international regulations. Several antibodies-based ELISAs have been develop to track gluten traces in food products. Among them, R5 and G12 antibody-based ELISAs are the frequently used methods. However, these antibodies have certain limitations. We evaluated the accuracy of G12/A1 antibody-based 'Glutentox ELISA Rapid G12' and compared the results with the current reference method i.e., R5 antibody-based 'Ridascreen R5 ELISA'. Methods: In the first step, the performance of Glutentox ELISA Rapid G12 kit was inspected by determination of the threshold value i.e., > or <20 mg/kg gluten in different food products. In the second step, quantification accuracy was assessed by quantification of gluten in gluten-free food products spiked with gliadin reference material. Results: In total 47 food products (naturally and labeled gluten-free, and food with traces of gluten) were included. Of them, 29 products were quantified with <20 mg/kg, and 18 with a low level of gluten by both the kits. Six out of 29 gluten-free products were used for the recovery test at different spike levels. Gluten concentration and mean recovery rates of individual kits showed consistency. Conclusion: GlutenTox Rapid G12 ELISA could be an appropriate choice for detecting gluten in food products but needs more in-house validation and collaborative tests.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/327712

Disease course of ulcerative proctitis in children: A population-based study on behalf of the SIGENP IBD Group
DIGESTIVE AND LIVER DISEASE
Autore/i: Martinelli, Massimo; Fedele, Flora; Romano, Claudio; Aloi, Marina; Lionetti, Paolo; Alvisi, Patrizia; Arrigo, Serena; Bosa, Luca; Bramuzzo, Matteo; D'Arcangelo, Giulia; Dipasquale, Valeria; Felici, Enrico; Fuoti, Maurizio; Gatti, Simona; Graziano, Francesco; Illiceto, Maria Teresa; Labriola, Flavio; Norsa, Lorenzo; Pastore, Maria; Scarallo, Luca; Strisciuglio, Caterina; Zuin, Giovanna; Miele, Erasmo; Staiano, Annamaria
Classificazione: 1 Contributo su Rivista
Abstract: Background: The natural history of ulcerative proctitis (UP) has been poorly investigated in children. Aims: We aimed to compare the disease course of children with UP at diagnosis to the other locations and to identify extension predictors. Methods: This was a multicenter, observational study carried out from data prospectively entered in the SIGENP-IBD-Registry. Children with ulcerative colitis (UC) diagnosis and at least 1-year follow-up were included. On the basis of Paris classification UP patients were identified and compared with the other locations. Results: 872 children were enrolled (median age at diagnosis: 11.2 years; M/F: 426/446), of whom 78 (9%) with UP. Kaplan-Meier analysis demonstrated increased cumulative probabilities of disease extension in the E1 group [1 year: 20.3%; 5 years: 52.7%; 10 years: 72.4%] compared to E3 group [1 year: 8.5%; 5 years: 24.9% and 10 years: 60.1%, p=0.001]. No differences were observed comparing E1 and E2 groups [p=0.4]. Cumulative probabilities of surgery at 1, 5 and 10 years were 1.3, 2.8 and 2.8% in the E1 group and 2.5, 8 and 12.8% in the E2-E3-E4 group, respectively (p=0.1). Cox regression analysis demonstrated that PUCAI>35 at diagnosis was associated with endoscopic extension (HR=4.9; CI 95% 1.5-15.2, p=0.006). Conclusions: UP is associated with similar short and long-term outcomes compared to other locations.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/327715

Trends and challenges in home enteral feeding methods for children with gastrointestinal disorders: an expert review on bolus feeding delivery methods
EXPERT REVIEW OF GASTROENTEROLOGY & HEPATOLOGY
Autore/i: Romano, Claudio; Lionetti, Paolo; Spagnuolo, Maria Immacolata; Amarri, Sergio; Diamanti, Antonella; Verduci, Elvira; Lezo, Antonella; Gatti, Simona
Classificazione: 1 Contributo su Rivista
Abstract: IntroductionNew evidence supports the benefits of bolus feeding for children receiving home enteral feeding (HEN). Current home methods of bolus feeding have certain limitations, particularly in mobile or restless patients. Therefore, innovative delivery methods have been introduced to provide more flexible methods of reducing feeding time and formula handling.Areas coveredThis manuscript presents an expert review of the updates in HEN for children and the results of an online user experience questionnaire about an innovative new cap-based bolus feeding system. A literature bibliographic search was conducted on Medline via PubMed up to September 2023 to collect relevant studies. We presented recent evidence demonstrating a dramatic increase in HEN use among children requiring EN and its benefits on patients' nutritional status and quality of life. In addition, the article examined the clinical and social benefits of bolus feeding and current challenges in delivery methods. We described the benefits of the new system and its user experience.Expert opinionThe uses and indications for bolus feeding in HEN are increasing among children. However, there are still some unmet needs regarding traditional delivery methods. Innovative techniques can improve flexibility, reduce feeding time, and improve user experience and quality of life.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/327717

Effects of the supplementation with a multispecies probiotic on clinical and laboratory recovery of children with newly diagnosed celiac disease: A randomized, placebo-controlled trial
DIGESTIVE AND LIVER DISEASE
Autore/i: Lionetti, Elena; Dominijanni, Vera; Iasevoli, Mario; Cimadamore, Elisa; Acquaviva, Ilaria; Gatti, Simona; Monachesi, Chiara; Catassi, Giulia; Pino, Alessandra; Faragalli, Andrea; Randazzo, Cinzia Lucia; Gesuita, Rosaria; Malamisura, Basilio; Catassi, Carlo
Classificazione: 1 Contributo su Rivista
Abstract: Objective: To evaluate the efficacy of a multispecies probiotic on clinical and laboratory recovery of children with celiac disease (CeD) at diagnosis. Methods: Children with newly diagnosed CeD entered a randomized double-blind placebo-controlled trial. A gluten-free diet (GFD) plus a multispecies probiotic or placebo were administered for 12 weeks. Growth, laboratory, and clinical parameters were recorded at enrollment, after 3 and 6 months of follow-up. Results: Overall, 96 children completed the study: 49 in group A (placebo) and 47 in group B (probiotic). A significant increase of BMI-Z score was found in both groups after 3 and 6 months of treatment (p < 0.001), however the increase of BMI-Z score was significantly higher and faster in Group B than in Group A. Other clinical and laboratory parameters improved in both groups after 3 and 6 months (p<0.001), but no difference was found between the groups and a comparable time trend was observed in both groups. Conclusions: Treatment with a multispecies probiotic induced a higher and faster increase of BMI in children with newly diagnosed CeD. The mechanism of this positive effect remains to be elucidated.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/315502

Vitamin D status in healthy Italian school-age children: a single-center cross-sectional study
THE ITALIAN JOURNAL OF PEDIATRICS
Autore/i: Galeazzi, Tiziana; Quattrini, Sara; Pjetraj, Dorina; Gatti, Simona; Monachesi, Chiara; Franceschini, Elisa; Marinelli, Luisita; Catassi, Giulia N; Lionetti, Elena; Catassi, Carlo
Classificazione: 1 Contributo su Rivista
Abstract: Background: Vitamin D is involved in calcium homeostasis and bone metabolism, although its extra-skeletal actions are also well-known. Low serum 25(OH)D levels are common both in adults and children worldwide. Methods: The purpose of this cross-sectional study was to determine the distribution of 25(OH)D levels in a cohort of healthy Italian school-age children, aged 5-10 years, in relationship to determinants of vitamin D deficiency such as season, BMI, gender, age and ethnicity. Results: The mean serum 25(OH) D level was 28.2 ng/mL; the prevalence of 25(OH)D sufficiency (> 30 ng/mL), insufficiency (20-30 ng/mL), deficiency (10-20 ng/mL) and severe deficiency (< 10 ng/mL) was 36%, 37%, 21% and 6% of the study-group population, respectively. The lower serum 25(OH)D values were observed during winter (21.6 ng/mL) and spring (22.9 ng/mL), as compared to summer (46.7 ng/mL) (p < 0.001). Higher BMI z-scores were associated with lower 25(OH)D level while no statistical difference was observed as related to gender and age groups. Conclusions: Healthy Italian schoolchildren show low 25(OH)D levels, particularly during winter and spring time. Seasonality, ethnicity and overweight/obesity were confirmed to influence the vitamin D status, thus indicating the need for effective initiatives to support adequate vitamin D status in this population group.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/315795

Hypertrophic Pyloric Stenosis in an Adolescent Girl: An Atypical Presentation of an Unexpected Disease
DISEASES
Autore/i: Gatti, Simona; Piloni, Francesca; Bindi, Edoardo; Cruccetti, Alba; Catassi, Carlo; Cobellis, Giovanni
Classificazione: 1 Contributo su Rivista
Abstract: : Hypertrophic pyloric stenosis is a common cause of vomiting in the first few weeks of life, but in rare cases, it may occur in older subjects with a major risk of delayed diagnosis and complications. We describe the case of a 12-year-and-8-month-old girl who presented to our department for epigastric pain, coffee-ground emesis, and melena, which arose after taking ketoprofen. An abdomen ultrasound showed thickening (1 cm) of the gastric pyloric antrum, while upper-GI endoscopy documented esophagitis and antral gastritis with a non-bleeding pyloric ulcer. During her hospital stay, she had no further episodes of vomiting and was therefore discharged with a diagnosis of "NSAIDs-induced acute upper gastrointestinal tract bleeding". After 14 days, following recurrence of abdominal pain and vomiting, she was hospitalized again. At endoscopy, pyloric sub-stenosis was found, abdominal CT showed thickening of large gastric curvature and pyloric walls, and an Rx barium study documented delayed gastric emptying. On suspicion of idiopathic hypertrophic pyloric stenosis, she underwent Heineke-Mikulicz pyloroplasty with resolution of symptoms and restoration of a regular caliber of the pylorus. Hypertrophic pyloric stenosis, although occurring rarely in older children, should be taken into account in the differential diagnosis of recurrent vomiting at any age.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/312747

Very early onset of coronary artery aneurysm in a 3-month infant with Kawasaki disease: a case report and literature review
THE ITALIAN JOURNAL OF PEDIATRICS
Autore/i: Jiao, Wenyan; Wei, Li; Jiao, Fuyong; Pjetraj, Dorina; Feng, Jianying; Wang, Jvyan; Catassi, Carlo; Gatti, Simona
Classificazione: 1 Contributo su Rivista
Abstract: Background: Kawasaki disease (KD) is a medium vessel vasculitis, of unknown etiology, typically presenting in children younger than 5 years of age. Prolonged fever (at least five days) is a major clinical criterion of KD, while cardiac involvement may occur in up to 25% of patients, generally in the second week of the disease. Case presentation: We describe the case of KD developing in a 3-month infant, with an early occurrence of coronary artery aneurysm after only 3 days of fever, complicated by thrombosis, requiring aggressive treatments. Conclusions: Time of development of cardiac complications can be different in young infants with KD and both diagnostic criteria and treatment indications should be individualized in this class of age.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/316951

DNA methylation of the TPMT gene and azathioprine pharmacokinetics in children with very early onset inflammatory bowel disease
BIOMÉDECINE & PHARMACOTHÉRAPIE
Autore/i: Selvestrel, Davide; Stocco, Gabriele; Aloi, Marina; Arrigo, Serena; Cardile, Sabrina; Cecchin, Erika; Congia, Mauro; Curci, Debora; Gatti, Simona; Graziano, Francesco; Langefeld, Carl D; Lucafò, Marianna; Martelossi, Stefano; Martinelli, Massimo; Pagarin, Sofia; Scarallo, Luca; Stacul, Elisabetta Francesca; Strisciuglio, Caterina; Thompson, Susan; Zuin, Giovanna; Decorti, Giuliana; Bramuzzo, Matteo
Classificazione: 1 Contributo su Rivista
Abstract: Background: Thiopurine methyltransferase (TPMT) is a crucial enzyme for azathioprine biotransformation and its activity is higher in very early onset inflammatory bowel disease (VEO-IBD) patients than in adolescents with IBD (aIBD).Aims: The aims of this pharmacoepigenetic study were to evaluate differences in peripheral blood DNA methylation of the TPMT gene and in azathioprine pharmacokinetics in patients with VEO-IBD compared to aIBD.Methods: The association of age with whole genome DNA methylation profile was evaluated in a pilot group of patients and confirmed by a meta-analysis on 3 cohorts of patients available on the public functional genomics data repository. Effects of candidate CpG sites in the TPMT gene were validated in a larger cohort using pyro-sequencing. TPMT activity and azathioprine metabolites (TGN) were measured in patients' erythrocytes by HPLC and associated with patients' age group and TPMT DNA methylation.Results: Whole genome DNA methylation pilot analysis, combined with the meta-analysis revealed cg22736354, located on TPMT downstream neighboring region, as the only statistically significant CpG whose methylation increases with age, resulting lower in VEO-IBD patients compared to aIBD (median 9.6% vs 12%, p = 0.029). Pyrosequencing confirmed lower cg22736354 methylation in VEO-IBD patients (median 4.0% vs 6.0%, p = 4.6 x10- 5). No differences in TPMT promoter methylation were found. Reduced cg22736354 methylation was associated with lower TGN concentrations (rho = 0.31, p = 0.01) in patients with VEO-IBD and aIBD.Conclusion: Methylation of cg22736354 in TPMT gene neighborhood is lower in patients with VEO-IBD and is associated with reduced azathioprine inactivation and increased TGN concentrations.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/315796

Celiac Disease Detection Strategies: Poor Performance of the Case-Finding Policy
THE AMERICAN JOURNAL OF GASTROENTEROLOGY
Autore/i: Catassi, Giulia N; Pjetraj, Dorina; Gatti, Simona; Lionetti, Elena; Catassi, Carlo
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/327714

Prevalence and detection rate of celiac disease in Italy: Results of a SIGENP multicenter screening in school-age children
DIGESTIVE AND LIVER DISEASE
Autore/i: Lionetti, Elena; Pjetraj, Dorina; Gatti, Simona; Catassi, Giulia; Bellantoni, Antonella; Boffardi, Massimo; Cananzi, Mara; Cinquetti, Mauro; Francavilla, Ruggiero; Malamisura, Basilio; Montuori, Monica; Zuccotti, Gianvincenzo; Cristofori, Fernanda; Gaio, Paola; Passaro, Tiziana; Penagini, Francesca; Testa, Alessandra; Trovato, Chiara Maria; Catassi, Carlo
Classificazione: 1 Contributo su Rivista
Abstract: Background: Celiac disease is a common lifelong disorder. Recent studies indicate that the number of clinically detected cases has increased over the last decades, however little is known about changes in the prevalence and the detection rate of celiac disease. Aim: To evaluate the current prevalence and detection rate of celiac disease in Italy by a multicenter, mass screening study on a large sample of school-age children. Methods: children aged 5-11 years were screened at school by HLA-DQ2 and -DQ8 determination on a drop of blood in six Italian cities; total serum IgA and IgA anti-transglutaminase were determined in children showing HLA-DQ2 and/or -DQ8 positivity. Diagnosis of celiac disease was confirmed according to the European guidelines. Results: 5994 children were eligible, 4438 participated and 1873 showed predisposing haplotypes (42.2%, 95% CI=40.7-43.7). The overall prevalence of celiac disease was 1.65% (95% CI, 1.34%-2.01%). Only 40% of celiac children had been diagnosed prior to the school screening. Symptoms evoking celiac disease were as common in celiac children as in controls. Conclusion: In this multicenter study the prevalence of celiac disease in school-age Italian children was one of the highest in the world. Determination of HLA predisposing genotypes is an easy and fast first-level screening test for celiac disease. Without a mass screening strategy, 60% of celiac patients remain currently undiagnosed in Italy.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/327716

Urinary Gluten Peptide Determination: Results Are Results, Even When They Contradict Aprioristic Expectations
CLINICAL AND TRANSLATIONAL GASTROENTEROLOGY
Autore/i: Monachesi, Chiara; Verma, Anil K; Catassi, Giulia N; Franceschini, Elisa; Gatti, Simona; Gesuita, Rosaria; Lionetti, Elena; Catassi, Carlo
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/315799

Metabolic Bone Disease in Children with Intestinal Failure and Long-Term Parenteral Nutrition: A Systematic Review
NUTRIENTS
Autore/i: Gatti, Simona; Quattrini, Sara; Palpacelli, Alessandra; Catassi, Giulia N; Lionetti, Maria Elena; Catassi, Carlo
Classificazione: 1 Contributo su Rivista
Abstract: Metabolic bone disease (MBD) is a possible complication of intestinal failure (IF), with a multi-factorial pathogenesis. The reduction of bone density (BMD) may be radiologically evident before manifestation of clinical signs (bone pain, vertebral compression, and fractures). Diagnosis relies on dual-energy X-ray absorptiometry (DXA). Incidence and evolution of MBD are not homogeneously reported in children. The aim of this systematic review was to define the prevalence of MBD in IF children and to describe risk factors for its development. A comprehensive search of electronic bibliographic databases up to December 2021 was conducted. Randomized controlled trials; observational, cross-sectional, and retrospective studies; and case series published between 1970 and 2021 were included. Twenty observational studies (six case-control) were identified and mostly reported definitions of MBD based on DXA parameters. Although the prevalence and definition of MBD was largely heterogeneous, low BMD was found in up to 45% of IF children and correlated with age, growth failure, and specific IF etiologies. Data demonstrate that long-term follow-up with repeated DXA and calcium balance assessment is warranted in IF children even when PN dependence is resolved. Etiology and outcomes of MBD will be better defined by longitudinal prospective studies focused on prognosis and therapeutic perspectives.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/315800

Circulating microRNAs as novel non-invasive biomarkers of paediatric celiac disease and adherence to gluten-free diet
EBIOMEDICINE
Autore/i: Felli, C.; Baldassarre, A.; Uva, P.; Alisi, A.; Cangelosi, D.; Ancinelli, M.; Caruso, M.; Paolini, A.; Montano, A.; Silano, M.; Vincentini, O.; Catassi, C.; Lionetti, E.; Gatti, S.; Ferretti, F.; Masotti, A.
Classificazione: 1 Contributo su Rivista
Abstract: Background: Celiac Disease (CD) is a multifactorial autoimmune enteropathy (with a prevalence of approximately 1% worldwide) that exhibits a wide spectrum of clinical, serological and histological manifestations. For the diagnosis of paediatric CD, the gold standard is the combination of serological tests (with high TGA-IgA values greater than 10 times the upper limit of normal) and duodenal biopsy (with a positive TGA-IgA but low titer). Therefore, a diagnostic test that totally excludes an invasive approach has not been discovered so far and the discovery of novel biological markers would represent an undoubted advantage for the diagnosis of CD and prognostic evaluation. MicroRNAs (miRNAs), small non-coding RNAs (18–22 nucleotides) that regulate gene expression at post-transcriptional level and play important roles in many biological processes, represent a novel class of potential disease biomarkers. Their presence in biological fluids (i.e., serum, plasma, saliva, urine) provides the opportunity to employ circulating miRNAs as novel non-invasive biomarkers. Methods: In our prospective observational study, we examined the expression of circulating miRNAs in a cohort of CD patients (both at diagnosis and on gluten-free diet, respectively referred as CD and GFD) compared to healthy controls. By small RNA-Seq we discovered a set of circulating miRNAs that were further validated by qPCR with specific assays. Findings: We found that out of the 13 miRNAs able to discriminate the three groups (i.e., CD, GFD and controls), three of them, namely miR-192-5p, miR-215-5p and miR-125b-5p (alone or in combination), were able to discriminate these three groups with high accuracy and specificity. Interpretation: Our conclusions emphasize that these circulating miRNAs can be employed not only for the diagnosis of CD patients with a low TGA-IgA titer but also to monitor the adherence to a gluten-free diet by CD patients. In conclusion, we suggest the use of the circulating miRNAs identified in this work as a novel diagnostic and follow-up tool for paediatric CD. Funding: This work was supported by Fondazione Celiachia Onlus (FC) Grant n° 018/FC/2013 and by Italian Ministry of Health (Ricerca Corrente).
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/296943

Cyclic vomiting syndrome in children: a nationwide survey of current practice on behalf of the Italian Society of Pediatric Gastroenterology, Hepatology and Nutrition (SIGENP) and Italian Society of Pediatric Neurology (SINP)
THE ITALIAN JOURNAL OF PEDIATRICS
Autore/i: Isoldi, Sara; Di Nardo, Giovanni; Mallardo, Saverio; Parisi, Pasquale; Raucci, Umberto; Tambucci, Renato; Quitadamo, Paolo; Salvatore, Silvia; Felici, Enrico; Cisarò, Fabio; Pensabene, Licia; Banzato, Claudia; Strisciuglio, Caterina; Romano, Claudio; Fusco, Patrizia; Rigotti, Francesca; Sansotta, Naire; Caimmi, Silvia; Savasta, Salvatore; Zuin, Giovanna; Di Stefano, Marina; Provera, Silvia; Campanozzi, Angelo; Rossi, Paolo; Gatti, Simona; Corpino, Mara; Alvisi, Patrizia; Martelossi, Stefano; Suppiej, Agnese; Gandullia, Paolo; Verrotti, Alberto; Terrin, Gianluca; Pacenza, Caterina; Fornaroli, Fabiola; Comito, Donatella; D'Arrigo, Stefano; Striano, Pasquale; Raviglione, Federico; Carotenuto, Marco; Orsini, Alessandro; Belcastro, Vincenzo; Di Corcia, Giovanna; Raieli, Vincenzo; Ferilli, Michela Ada Noris; Ruscitto, Claudia; Spadoni, Elisabetta; Grosso, Salvatore; D'Alonzo, Renato; Papa, Amanda; Pavone, Piero; Meli, Mariaclaudia; Velardita, Mario; Mainetti, Martina; Vanacore, Nicola; Borrelli, Osvaldo
Classificazione: 1 Contributo su Rivista
Abstract: Background Cyclic Vomiting Syndrome (CVS) is a rare functional gastrointestinal disorder, which has a considerable burden on quality of life of both children and their family. Aim of the study was to evaluate the diagnostic modalities and therapeutic approach to CVS among Italian tertiary care centers and the differences according to subspecialties, as well as to explore whether potential predictive factors associated with either a poor outcome or a response to a specific treatment. Methods Cross-sectional multicenter web-based survey involving members of the Italian Society of Pediatric Gastroenterology, Hepatology and Nutrition (SIGENP) and Italian Society of Pediatric Neurology (SINP). Results A total of 67 responses were received and analyzed. Most of the respondent units cared for less than 20 patients. More than half of the patients were referred after 3 to 5 episodes, and a quarter after 5 attacks. We report different diagnostic approaches among Italian clinicians, which was particularly evident when comparing gastroenterologists and neurologists. Moreover, our survey demonstrated a predilection of certain drugs during emetic phase according to specific clinic, which reflects the cultural background of physicians. Conclusion In conclusion, our survey highlights poor consensus amongst clinicians in our country in the diagnosis and the management of children with CVS, raising the need for a national consensus guideline in order to standardize the practice.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/315798

Induction of Remission With Exclusive Enteral Nutrition in Children With Crohn's Disease: Determinants of Higher Adherence and Response
INFLAMMATORY BOWEL DISEASES
Autore/i: Cuomo, Marialaura; Carobbio, Alessandra; Aloi, Marina; Alvisi, Patrizia; Banzato, Claudia; Bosa, Luca; Bramuzzo, Matteo; Campanozzi, Angelo; Catassi, Giulia; D'Antiga, Lorenzo; Di Paola, Monica; Felici, Enrico; Fioretti, Maria Teresa; Gatti, Simona; Graziano, Francesco; Lega, Sara; Lionetti, Paolo; Marseglia, Antonio; Martinelli, Massimo; Musto, Francesca; Sansotta, Naire; Scarallo, Luca; Zuin, Giovanna; Norsa, Lorenzo
Classificazione: 1 Contributo su Rivista
Abstract: Background Exclusive enteral nutrition (EEN) is the first choice to induce remission and promote mucosal healing in pediatric Crohn's disease (CD). However, full adherence to EEN treatment may be problematic for children with CD. Methods The goal of the current multicenter retrospective study was to define predictive factors of nonadherence to treatment and nonremission at the end of induction treatment. Those data together were analyzed with the ultimate goal of trying to define an individualized induction treatment for children with CD. Results Three hundred seventy-six children with CD from 14 IBD pediatric referral centers were enrolled in the study. The rate of EEN adherence was 89%. Colonic involvement and fecal calprotectin >600 mu g/g at diagnosis were found to be associated with a reduced EEN adherence. Exclusive enteral nutrition administered for 8 weeks was effective for inducing clinical remission in 67% of the total cohort. Factors determining lower remission rates were age >15 years and Pediatric Crohn's Disease Activity Index >50. Conclusion Although EEN is extremely effective in promoting disease remission, several patients' related factors may adversely impact EEN adherence and response. Personalized treatments should be proposed that weigh benefits and risks based on the patient's disease location, phenotype, and disease activity and aim to promote a rapid control of inflammation to reduce long-term bowel damage.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/315797

Real-Life Use of Biosimilars in Pediatric Inflammatory Bowel Disease: A Nation-Wide Web Survey on Behalf of the SIGENP IBD Working Group
PAEDIATRIC DRUGS
Autore/i: Dipasquale, V.; Martinelli, M.; Aloi, M.; Accomando, S.; Alvisi, P.; Arrigo, S.; Banzato, C.; Bramuzzo, M.; Cananzi, M.; Corpino, M.; Di Mari, C.; Di Nardo, G.; Dilillo, A.; Dodaro, N.; Felici, E.; Gatti, S.; Graziano, F.; Illiceto, M. T.; Norsa, L.; Opramolla, A.; Pastore, M.; Pavanello, P. M.; Romeo, E.; Scarallo, L.; Strisciuglio, C.; Zuin, G.; Romano, C.
Classificazione: 1 Contributo su Rivista
Abstract: Objective: The aim was to assess the awareness and real-life use of biosimilars in inflammatory bowel disease (IBD) among the members of the Italian Society of Pediatric Gastroenterology, Hepatology and Nutrition (SIGENP). Methods: An anonymous web survey involving all SIGENP IBD units which can prescribe biosimilars was conducted between July 1st and December 1st, 2020. The questionnaire included 18 questions addressing the most relevant aspects of biosimilars in pediatric IBD, i.e., advantages, disadvantages, costs, traceability, general knowledge, and real-life use. A descriptive analysis of responses was performed. Results: Responses came from 26 pediatric IBD units in Italy, with representation of the North, the Center, and the South of Italy. The majority of participants (n = 20) had spent > 10 years caring for pediatric IBD patients, and worked in a center which had between 100 and 500 registered pediatric IBD patients (n = 14). Most participants (n = 18) reported they were aware that biosimilars have similar efficacy and safety to those of the originator, and all regarded cost-sparing as the main advantage of biosimilars. Most respondents (n = 20) reported they switch from originator to biosimilar in their everyday clinical practice, mostly during the maintenance phase. Most respondents (n = 20) registered no acute adverse events. Nearly all participants felt totally or very confident in using biosimilars. Conclusions: A few years after the introduction of the first biosimilar into the market, real-life data coming from the major IBD units in Italy confirm a favorable and confident position on the use of biosimilars in pediatric IBD.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/294851

Disease Activity Patterns in the First 5 Years after Diagnosis in Children with Ulcerative Colitis: A Population-Based Study
JOURNAL OF CROHN'S AND COLITIS
Autore/i: Aloi, M.; Bramuzzo, M.; Norsa, L.; Arrigo, S.; Distante, M.; Miele, E.; Romano, C.; Giobbi, C.; Panceri, R.; Cucchiara, S.; Alvisi, P.; Lombardi, G.; Salvatore, S.; Gatti, S.; De Giacomo, C.; Knafelz, D.; Barera, G.; Provera, S.; Pastore, M.
Classificazione: 1 Contributo su Rivista
Abstract: Background: The aim of this study was to define clusters of activity in a population-based cohort during the first 5 years after diagnosis in children with ulcerative colitis [UC] and to identify early prognostic risk factors. Methods: All UC patients from the SIGENP IBD registry with a complete follow-up of at least 5 years were included. Active disease was defined every 6 months in the presence of at least one of the following: clinical activity [Paediatric Ulcerative Colitis Activity Index ≥ 35]; endoscopic activity [Mayo score ≥ 1]; faecal calprotectin > 250 μg/g; hospitalization; surgery; or treatment escalation. Formula-based clusters were generated based on four published questionnaire-based activity patterns in adults, plus one additional cluster. Results: In total, 226 patients were identified. Forty-two [19%] had moderate-severe chronically active disease, 31 [14%] chronic-intermittent, 75 [33%] quiescent, 54 [24%] active disease in the first 2 years after the diagnosis, then sustained remission, and 24 [11%] a remission in the first 2 years then an active disease. Mild disease onset along with a lower clinical severity not requiring the use of corticosteroids at 6 months were related to a quiescent disease course at the next follow-up (logistic model area under the curve 0.86 [95% confidence interval 0.78-0.94]; positive predictive value 67%; negative predictive value 70%). Eight per cent of patients needed surgery, none in the quiescent group [p = 0.04]. Conclusions: More than one-third of children with UC present with a chronically active or intermittent course during the first 5 years of follow-up. A significant group of patients has active disease in the first 2 years and then sustained remission. Interestingly, after initial treatment, one-third of patients have well-controlled disease throughout.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/294856

Factors associated with quality of life in Italian children and adolescents with IBD
SCIENTIFIC REPORTS
Autore/i: Gatti, Simona; Del Baldo, Giada; Catassi, Giulia; Faragalli, Andrea; Aloi, Marina; Bramuzzo, Matteo; D'Arcangelo, Giulia; Felici, Enrico; Fuoti, Maurizio; Lega, Sara; Panceri, Roberto; Pastore, Maria; Penagini, Francesca; Gesuita, Rosaria; Catassi, Carlo
Classificazione: 1 Contributo su Rivista
Abstract: Improving the quality of life (QoL) is crucial in the management of pediatric inflammatory bowel disease (IBD). We aimed to (1) Validate the IMPACT-III questionnaire in Italian IBD children; (2) explore factors associated to QoL in pediatric IBD. Internal consistency, concurrent validity, discriminant validity and reproducibility of the Italian version of the IMPACT-III questionnaire was measured in IBD children/adolescents in 8 centers. Associations between patient and disease characteristics and the IMPACT-III domains were analyzed through quantile regression analysis. The IMPACT-III questionnaire, collected in 282 children with IBD (median age: 14.8 years; IQR 12.4-16.4) showed a median total score of 76 (IQR 67-83). Female gender, active disease and age were negatively associated with the total IMPACT-III score. Specifically, female gender was negatively associated with the Bowel/Systemic Symptoms, Emotional and Treatment domain scores, while disease activity was significantly associated with Bowel Symptoms and Treatment/Interventions reported QoL. The IMPACT- III showed good internal consistency (Cronbach's alpha coefficient = 0.87, 95% CI 0.85-0.89) and reproducibility (Concordance Correlation Coefficient = 0.66, 95% CI 0.57-0.74). In Italian children with IBD active disease, female gender and adolescence are associated to a worse QoL, indicating the need of more attention in this subgroup of young patients. IMPACT-III questionnaire is a reliable instrument to measure QoL in Italian children.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/307610

The Clinical Spectrum of Inflammatory Bowel Disease Associated With Specific Genetic Syndromes: Two Novel Pediatric Cases and a Systematic Review
FRONTIERS IN PEDIATRICS
Autore/i: Gatti, S.; Gelzoni, G.; Catassi, G. N.; Catassi, C.
Classificazione: 1 Contributo su Rivista
Abstract: Background and Aims: Inflammatory bowel disease (IBD) is a typical polygenic disorder and less frequently shows a monogenic origin. Furthermore, IBD can originate in the context of specific genetic syndromes associated with a risk of autoimmune disorders. We aimed to systematically evaluate the prevalence of IBD in specific genetic syndromes and to review the clinical characteristics of the published cases. Methods: According to the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines, studies describing patients with IBD and a genetic syndrome and/or studies indicating the prevalence or incidence of IBD in subjects with a genetic syndrome were included. Results: Forty-six studies describing a total of 67 cases of IBD in six genetic syndromes and two personally assessed unpublished cases were included in the review. The majority of cases were associated with Turner syndrome (TS) (38 cases), Down syndrome (DS) (18 cases) and neurofibromatosis type 1 (NF1) (8 cases). Sporadic cases were described in DiGeorge syndrome (2), Kabuki syndrome (2), and Williams syndrome (1). The prevalence of IBD ranged from 0.67 to 4% in TS and from 0.2 to 1.57% in DS. The incidence of IBD was increased in TS and DS compared to the general population. Eight cases of IBD in TS had a severe/lethal course, many of which described before the year 2000. Two IBD cases in DS were particularly severe. Conclusion: Evidence of a greater prevalence of IBD is accumulating in TS, DS, and NF1. Management of IBD in patients with these genetic conditions should consider the presence of comorbidities and possible drug toxicities. Systematic Review Registration: PROSPERO, identifier: CRD42021249820.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/294852

Current status and perspectives on the application of crispr/cas9 gene-editing system to develop a low-gluten, non-transgenic wheat variety
FOODS
Autore/i: Verma, A. K.; Mandal, S.; Tiwari, A.; Monachesi, C.; Catassi, G. N.; Srivastava, A.; Gatti, S.; Lionetti, E.; Catassi, C.
Classificazione: 1 Contributo su Rivista
Abstract: Wheat gluten contains epitopes that trigger celiac disease (CD). A life-long strict gluten-free diet is the only treatment accepted for CD. However, very low-gluten wheat may provide an alternative treatment to CD. Conventional plant breeding methods have not been to produce celiac-safe wheat. RNA interference technology, to some extent, has succeeded in the development of safer wheat varieties. However, these varieties have multiple challenges in terms of their implementation. Clustered Regularly Interspaced Short Palindromic Repeats-associated nuclease 9 (CRISPR/Cas9) is a versatile gene-editing tool that has the ability to edit immunogenic gluten genes. So far, only a few studies have applied CRISPR/Cas9 to modify the wheat genome. In this article, we reviewed the published literature that applied CRISPR/Cas9 in wheat genome editing to investigate the current status of the CRISPR/Cas9 system to produce a low-immunogenic wheat variety. We found that in recent years, the CRISPR/Cas9 system has been continuously improved to edit the complex hexa-ploid wheat genome. Although some reduced immunogenic wheat varieties have been reported, CRISPR/Cas9 has still not been fully explored in terms of editing the wheat genome. We conclude that further studies are required to apply the CRISPR/Cas9 gene-editing system efficiently for the development of a celiac-safe wheat variety and to establish it as a “tool to celiac safe wheat.”.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/294853

Determination of Urinary Gluten Immunogenic Peptides to Assess Adherence to the Gluten-Free Diet: A Randomized, Double-Blind, Controlled Study
CLINICAL AND TRANSLATIONAL GASTROENTEROLOGY
Autore/i: Monachesi, C.; Verma, A. K.; Catassi Naspi, Giulia; Franceschini, E.; Gatti, S.; Gesuita, R.; Lionetti, E.; Catassi, C.
Classificazione: 1 Contributo su Rivista
Abstract: INTRODUCTION: The adherence to a gluten-free diet (GFD) is a trending topic in the management of celiac disease. The aim of our study was to evaluate the diagnostic performance of urinary gluten immunogenic peptides (GIP) determination to detect gluten contamination of the GFD. METHODS: In study A, 25 healthy adults on a standard GFD performed 6 gluten challenges (0, 10, 50, 100, 500, and 1,000 mg) with quantification of urinary GIP before (T0) and during the following 24 hours. In study B, 12 participants on a gluten contamination elimination diet underwent urinary GIP determination at T0 and after challenge with 5 or 10 mg gluten. Urine GIP concentration was determined by an immunochromatographic assay. RESULTS: In study A, 51 of 150 baseline urine samples were GIP+ on GFD and 7 of 17 were GIP+ after the zero-gluten challenge, whereas only 55 of 81 were GIP+ after the 10-1,000 mg gluten challenges. There was no significant change in the 24-hour urinary GIP when increasing gluten from 10 to 1,000 mg. In study B, 24 of 24 baseline urine samples were GIP-, whereas 8 of 24 were GIP+ after 5 or 10 mg of gluten. DISCUSSION: Traces of gluten in the standard GFD may cause positivity of urinary GIP determination, whereas a false negativity is common after a gluten intake of 10-1,000 mg. Owing to the impossibility of standardizing the test in normal conditions, it seems unlikely that urinary GIP determination may represent a reliable tool to assess the compliance to the GFD of patients with celiac disease or other gluten-related disorders.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/294854

Management of paediatric IBD after the peak of COVID-19 pandemic in Italy: A position paper on behalf of the SIGENP IBD working group
DIGESTIVE AND LIVER DISEASE
Autore/i: Arrigo, S.; Alvisi, P.; Banzato, C.; Bramuzzo, M.; Civitelli, F.; Corsello, A.; D'Arcangelo, G.; Dilillo, A.; Dipasquale, V.; Felici, E.; Fuoti, M.; Gatti, S.; Giusti, Z.; Knafelz, D.; Lionetti, P.; Mario, F.; Marseglia, A.; Martelossi, S.; Moretti, C.; Norsa, L.; Nuti, F.; Panceri, R.; Rampado, S.; Renzo, S.; Romano, C.; Romeo, E.; Strisciuglio, C.; Martinelli, M.
Classificazione: 1 Contributo su Rivista
Abstract: Coronavirus Disease 2019 (COVID-19) caused by severe acute respiratory syndrome coronavirus 2, spreading in Italy during the first months of 2020, abruptly changed the way of practicing medicine in this country. As a consequence of the lockdown, the diagnostic and therapeutic management of paediatric chronic conditions, such as inflammatory bowel disease (IBD) has been affected. During the peak of COVID-19 pandemic, elective visits, endoscopies and infusions have been postponed, with potential clinical and psychological impact on disease course and a high likelihood of increasing waiting lists. While slowly moving back towards normality, clinicians need to recognize the best ways to care for patients with IBD, carefully avoiding risk factors for new potential epidemic outbreaks. In this uncertain scenario until the development and spread of COVID-19 vaccine, it is necessary to continue to operate with caution. Hereby we provide useful indications for a safer and gradual restarting of routine clinical activities after COVID-19 peak in Italy.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/294858

Impact of COVID-19 pandemic on the management of paediatric inflammatory bowel disease: An Italian multicentre study on behalf of the SIGENP IBD Group
DIGESTIVE AND LIVER DISEASE
Autore/i: Arrigo, S.; Alvisi, P.; Banzato, C.; Bramuzzo, M.; Celano, R.; Civitelli, F.; D'Arcangelo, G.; Dilillo, A.; Dipasquale, V.; Felici, E.; Fuoti, M.; Gatti, S.; Knafelz, D.; Lionetti, P.; Mario, F.; Marseglia, A.; Martelossi, S.; Moretti, C.; Norsa, L.; Panceri, R.; Renzo, S.; Romano, C.; Romeo, E.; Strisciuglio, C.; Martinelli, M.
Classificazione: 1 Contributo su Rivista
Abstract: Background: IBD management has been significantly affected during the COVID-19 lockdown with potential clinical issues. Aims: The aim of this study was to analyse the impact of COVID-19 pandemic on the Italian paediatric IBD cohort. Methods: This was a multicentre, retrospective, cohort investigation including 21 different Italian IBD referral centres. An electronic data collection was performed among the participating centres including: clinical characteristics of IBD patients, number of COVID-19 cases and clinical outcomes, disease management during the lockdown and the previous 9 weeks. Results: 2291 children affected by IBD were enrolled. We experienced a significant reduction of the hospital admissions [604/2291 (26.3%) vs 1281/2291 (55.9%); p < 0.001]. More specifically, we observed a reduction of hospitalizations for new diagnosis (from n = 44 to n = 27) and endoscopic re-evaluations (from n = 46 to n = 8). Hospitalization for relapses and surgical procedures remained substantially unchanged. Biologic infusions did not significantly vary [393/2291 (17.1%) vs 368/2291 (16%); p = 0.3]. Telemedicine services for children with IBD were activated in 52.3% of the centres. In 42/2291(1.8%) children immunosuppressive therapies were adapted due to the concurrent COVID-19 pandemic. Conclusion: Due to the several limitations of the lockdown, cares for children with IBD have been kept to minimal standards, giving priorities to the urgencies and to biologics’ infusions and implementing telemedicine services.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/294857

Slow Decrease of Antitissue Transglutaminase Antibody Positivity in Children With Celiac Disease After Starting the Gluten-free Diet
JOURNAL OF PEDIATRIC GASTROENTEROLOGY AND NUTRITION
Autore/i: Monachesi, C.; Verma, A. K.; Catassi, G. N.; Gatti, S.; Lionetti, E.; Catassi, C.
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/286108

Nutritional status, dietary intake, and adherence to the mediterranean diet of children with celiac disease on a gluten-free diet: A case-control prospective study
NUTRIENTS
Autore/i: Lionetti, E.; Antonucci, N.; Marinelli, M.; Bartolomei, B.; Franceschini, E.; Gatti, S.; Catassi, G. N.; Verma, A. K.; Monachesi, C.; Catassi, C.
Classificazione: 1 Contributo su Rivista
Abstract: The only effective treatment for celiac disease (CD) is a life-long strict gluten-free diet (GFD). Nutritional adequacy of the GFD has remained controversial and a matter of debate for a long time. No large case-control studies on children regarding the nutritional adequacy of the GFD has been performed. In this study, children diagnosed with CD on a GFD for ≥ 2 years were recruited. Controls were age and gender-matched healthy children not affected with CD. In both groups, anthropometric measurements and energy expenditure information were collected. Dietary assessment was performed by a 3-day food diary. Adherence to the Mediterranean diet was estimated by the KIDMED index. Overall, 120 children with CD and 100 healthy children were enrolled. No differences were found between CD children and controls in anthropometric measurements and energy expenditure. In the CD group, the daily intake of fats was significantly higher while the consumption of fiber was lower in comparison with the control group. The median KIDMED index was 6.5 in CD children and 6.8 in healthy controls. The diet of children with CD in this study was nutritionally less balanced than controls, with a higher intake of fat and a lower intake of fiber, highlighting the need for dietary counseling.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/294913

Increased Prevalence of Celiac Disease in School-age Children in Italy
CLINICAL GASTROENTEROLOGY AND HEPATOLOGY
Autore/i: Gatti, Simona; Lionetti, Elena; Balanzoni, Linda; Verma, Anil K; Galeazzi, Tiziana; Gesuita, Rosaria; Scattolo, Novella; Cinquetti, Mauro; Fasano, Alessio; Catassi, Carlo
Classificazione: 1 Contributo su Rivista
Abstract: Background & Aims: Celiac disease is one of the most common diseases worldwide, with an apparent trend of increasing prevalence. We investigated the prevalence of celiac disease in children in Italy in 2015–2016 and compared that with data from 25 years ago. Methods: We screened 4570 children (5–11 years old, 80.1% of the eligible population) from metropolitan areas of Ancona and Verona for HLA genes associated with increased risk of celiac disease, and for total serum levels of IgA and IgA class anti-tissue transglutaminase in HLA positives. Diagnoses of celiac disease were confirmed by detection of anti-endomysial antibody and analysis of intestinal biopsies. The prevalence of celiac autoimmunity and celiac disease were calculated and compared with values from the same geographical area during the years 1993–1995, after adjustment for the different diagnostic algorithm. Results: We identified 1960 children with celiac disease-associated haplotypes (43% of children screened; 95% CI, 40.8%–45.2%). The prevalence of celiac disease autoimmunity in the HLA-positive subjects was 96/1706 (5.62%; 95% CI, 4.53%–6.71%) and 54 of these children satisfied the diagnostic criteria for celiac disease. In the eligible population there were other 23 known cases of celiac disease. The overall estimated prevalence of celiac disease was 1.58% (95% CI, 1.26%–1.90%); this value is significantly higher than the 1993–1995 adjusted prevalence (0.88%; 95% CI, 0.74%–1.02%). Conclusions: We found the prevalence of celiac disease in children in Italy to be greater than 1.5%; this value has increased significantly over the past 25 years. Studies are needed to determine the causes of this large increase.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/268507

The pediatric endoscopy practice in Italy: A nationwide survey on behalf of the Italian society of pediatric gastroenterology, hepatology and nutrition (SIGENP)
DIGESTIVE AND LIVER DISEASE
Autore/i: Deganello Saccomani, M.; Norsa, L.; Oliva, S.; De'Angelis, G. L.; Accomando, S.; Alvisi, P.; Balassone, V.; Bramuzzo, M.; Campanozzi, A.; Cavataio, F.; Centenari, C.; Chiaro, A.; Cisaro, F.; Citrano, M.; Costa, L.; Cozzali, R.; D'Adamo, G.; D'Altilia, M.; Di Chio, T.; Di Nardo, G.; Dodaro, N.; Falchetti, D.; Famiani, A.; Fanti, L.; Felici, E.; Francavilla, R.; Gandullia, P.; Gatti, S.; Granata, A.; Illiceto, M. T.; Maino, M.; Malaventura, C.; Mantegazza, C.; Martelossi, S.; Miele, E.; Monzani, A.; Muscas, A.; Nicastro, E.; Orizio, P.; Pacenza, C.; Paci, M.; Parma, B.; Raffaele, A.; Ravelli, A.; Romano, C.; Strisciuglio, C.
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/294859

Misuse of serological screening tests for celiac disease in children: A prospective study in Italy
DIGESTIVE AND LIVER DISEASE
Autore/i: Franceschini, Elisa; Lionetti, Maria Elena; D'Adamo, Grazia; D'Angelo, Elisa; Gatti, Simona; Naspi Catassi, Giulia; Malamisura, Basilio; Catassi, Carlo
Classificazione: 1 Contributo su Rivista
Abstract: Despite a well-established diagnostic algorithm for celiac disease, it remains unclear whether prescriptions for celiac serological tests comply with the current pediatric guidelines.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/270459

Validation of a novel single-drop rapid human leukocyte antigen-DQ2/-DQ8 typing method to identify subjects susceptible to celiac disease
JGH OPEN
Autore/i: Verma, A. K.; Singh, A.; Gatti, S.; Lionetti, E.; Galeazzi, T.; Monachesi, C.; Franceschini, E.; Ahuja, V.; Catassi, C.; Makharia, G. K.
Classificazione: 1 Contributo su Rivista
Abstract: Background and Aim: Human leukocyte antigen (HLA)-DQ2 and/or -DQ8 is an essential risk factor for celiac disease (CD). About 90–95% of patients with CD carry HLA-DQ2/-DQ8 alleles, and HLA-DQ typing is considered an additional diagnostic test. Conventional polymerase chain reaction (PCR)-based HLA-DQ typing methods are expensive, complex, and a time-consuming process. We assessed the efficacy of a novel HLA-DQ typing method, “Celiac Gene Screen,” for the detection of CD-associated HLA haplotypes. Methods: To assess the diagnostic performance of the Celiac Gene Screen test, 100 ethylenediaminetetraacetic acid (EDTA) blood samples, already characterized by the conventional HLA-DQ typing method, that is, PCR sequence-specific oligonucleotide probes (PCR-SSOP), a concordance between both the methods were explored. For validity, a further 300 EDTA blood samples with unknown HLA-DQ status were genotyped using the Celiac Gene Screen test, including 141 samples from CD, 56 first-degree relatives (FDRs) of CD and 103 samples from controls. Results: Of the 100 samples with known status of HLA-DQ alleles, 79 samples were HLA-DQ2 and/or -DQ8 positive, and 21 samples were HLA-DQ2 and/or -DQ8 negative by conventional PCR. These 100 samples were re-typed using the Celiac Gene screen kit; all 79 positives were typed positive, and 21 negatives were typed negative for HLA-DQ alleles. Among 300 samples with unknown HLA-DQ status, 118 of 141 (84%) patients with CD, 48 of 56 (86%) FDRs of CD, and 52 of 103 (50%) controls typed positive for HLA-DQ alleles. Conclusions: The Celiac Gene Screen HLA-DQ typing method showed excellent concordance with the conventional HLA-DQ typing method and could be a cost-reducing and effective method for CD-associated HLA screening.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/294919

Safety of Oats in Children with Celiac Disease: A Double-Blind, Randomized, Placebo-Controlled Trial
THE JOURNAL OF PEDIATRICS
Autore/i: Lionetti, E; Gatti, S; Galeazzi, T; Caporelli, Nicole; Francavilla, R; Cucchiara, Salvatore; Roggero, P; Malamisura, Basilio; Iacono, Giuseppe; Tomarchio, S; Kleon, W; Restani, P; Brusca, I; Budelli, A; Gesuita, R; Carle, F; Catassi, C.
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/254634

Validation of a novel single-drop rapid human leukocyte antigen-DQ2/-DQ8 typing method to identify subjects susceptible to celiac disease
JGH OPEN
Autore/i: Verma, Anil K; Singh, Alka; Gatti, Simona; Lionetti, Elena; Galeazzi, Tiziana; Monachesi, Chiara; Franceschini, Elisa; Ahuja, Vineet; Catassi, Carlo; Makharia, Govind K
Classificazione: 1 Contributo su Rivista
Abstract: Human leukocyte antigen (HLA)-DQ2 and/or -DQ8 is an essential risk factor for celiac disease (CD). About 90-95% of patients with CD carry HLA-DQ2/-DQ8 alleles, and HLA-DQ typing is considered an additional diagnostic test. Conventional polymerase chain reaction (PCR)-based HLA-DQ typing methods are expensive, complex, and a time-consuming process. We assessed the efficacy of a novel HLA-DQ typing method, "Celiac Gene Screen," for the detection of CD-associated HLA haplotypes.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/268506

Paediatric home artificial nutrition in Italy: Report from 2016 survey on behalf of artificial nutrition network of italian society for gastroenterology, hepatology and nutrition (SIGENP)
NUTRIENTS
Autore/i: Lezo, A.; Capriati, T.; Spagnuolo, M. I.; Lacitignola, L.; Goreva, I.; Di Leo, G.; Cecchi, N.; Gandullia, P.; Amarri, S.; Forchielli, M. L.; Dipasquale, V.; Parma, B.; Gatti, S.; Ravaioli, E.; Salvatore, S.; Mainetti, M.; Norsa, L.; Pellegrino, M.; Fornaro, M.; Fiorito, V.; Lanari, M.; Giaquinto, E.; Verduci, E.; Baldassarre, M. E.; Diamanti, A.
Classificazione: 1 Contributo su Rivista
Abstract: Home Artificial Nutrition (HAN) is a safe and efficacious technique that insures children’s reintegration into the family, society and school. Epidemiological data on paediatric HAN in Italy are not available. Aim: to detect the prevalence and incidence of Home Parenteral Nutrition (HPN) and Home Enteral Nutrition (HEN), either via tube or mouth, in Italy in 2016. Materials and methods: a specific form was sent to all registered SIGENP members and investigators of local HAN centres, inviting them to provide the requested centre’s data and demographics, underlying diseases and HAN characteristics of the patients. Results: we recorded 3403 Italian patients on HAN aged 0 to 19 years from 22 centres: 2277 HEN, 950 Oral Nutritional Supplements (ONS) and 179 HPN programs. The prevalence of HEN (205 pts/million inhabitants) and HPN (16 pts/million inhabitants) has dramatically increased in Italy in the last 9 years. Neurodisabling conditions were the first indication for HEN by tube or mouth while HPN is mainly requested in digestive disorders. Conclusions: HAN is a widespread and rapidly growing treatment in Italy, as well as in other European countries. Awareness of its extent and characteristics helps improving HAN service and patients’ quality of life.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/294860

Contribution of Oral Hygiene and Cosmetics on Contamination of Gluten-Free Diet: Do Celiac Customers Need to Worry About?
JOURNAL OF PEDIATRIC GASTROENTEROLOGY AND NUTRITION
Autore/i: Verma, Anil K; Lionetti, Elena; Gatti, Simona; Franceschini, Elisa; Catassi, Giulia Naspi; Catassi, Carlo
Classificazione: 1 Contributo su Rivista
Abstract: The only available treatment for celiac disease (CD) is the gluten-free diet. It is unclear whether the presence of gluten in oral hygiene products and cosmetics that are applied on the mouth is a reason of concern for CD patients. The aim of this study was to test the level of gluten contamination in oral hygiene and cosmetic products available in the Italian market.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/260033

Safety of Oats in Children with Celiac Disease: A Double-Blind, Randomized, Placebo-Controlled Trial
THE JOURNAL OF PEDIATRICS
Autore/i: Lionetti, E.; Gatti, S.; Galeazzi, T.; Caporelli, N.; Francavilla, R.; Cucchiara, S.; Roggero, P.; Malamisura, B.; Iacono, G.; Tomarchio, S.; Kleon, W.; Restani, P.; Brusca, I.; Budelli, A.; Gesuita, R.; Carle, F.; Catassi, C.
Classificazione: 1 Contributo su Rivista
Abstract: Objective: To evaluate the long-term validity and safety of pure oats in the treatment of children with celiac disease. Study design: This noninferiority clinical trial used a double-blind, placebo-controlled, crossover design extended over 15 months. Three hundred six children with a biopsy-proven diagnosis of celiac disease on a gluten-free diet for ≥2 years were randomly assigned to eat specifically prepared gluten-free food containing an age-dependent amount (15-40 g) of either placebo or purified nonreactive varieties of oats for 2 consecutive 6-month periods separated by washout standard gluten-free diet for 3 months. Clinical (body mass index, Gastrointestinal Symptoms Rating Scale score), serologic (IgA antitransglutaminase antibodies, and IgA anti-avenin antibodies), and intestinal permeability data were measured at baseline, and after 6, 9, and 15 months. Direct treatment effect was evaluated by a nonparametric approach using medians (95% CI) as summary statistic. Results: After the exclusion of 129 patients who dropped out, the cohort included 177 children (79 in the oats–placebo and 98 in the placebo–oats group; median, 0.004; 95% CI, −0.0002 to 0.0089). Direct treatment effect was not statistically significant for clinical, serologic, and intestinal permeability variables (body mass index: median, −0.5; 95% CI, −0.12 to 0.00; Gastrointestinal Symptoms Rating Scale score: median, 0; 95% CI, −2.5 to 0.00; IgA antitransglutaminase antibodies: median, −0.02; 95% CI, −0.25 to 0.23; IgA anti-avenin antibodies: median, −0.0002; 95% CI, −0.0007 to 0.0003; intestinal permeability test: median, 0.004; 95% CI, −0.0002 to 0.0089). Conclusions: Pure nonreactive oat products are a safe dietary choice in the treatment of children with celiac disease. Trial registration: ClinicalTrials.gov: NCT00808301.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/294914

Efficacy and Safety of Adalimumab in Pediatric Ulcerative Colitis: A Real-life Experience from the SIGENP-IBD Registry
JOURNAL OF PEDIATRIC GASTROENTEROLOGY AND NUTRITION
Autore/i: Aloi, M.; Bramuzzo, M.; Arrigo, S.; Romano, C.; D'Arcangelo, G.; Lacorte, D.; Gatti, S.; Illiceto, M. T.; Zucconi, F.; Dilillo, D.; Zuin, G.; Knafelz, D.; Ravelli, A.; Cucchiara, S.; Alvisi, P.
Classificazione: 1 Contributo su Rivista
Abstract: Objectives: The aim of this study was to evaluate the effectiveness and safety of adalimumab (ADA) in children with ulcerative colitis (UC) previously treated with infliximab (IFX). Methods: Retrospective study including children with UC from a national registry who received ADA therapy. The primary endpoint was the rate of corticosteroid-free remission at week 52. Secondary outcomes were the rate of sustained clinical remission, primary nonresponse, and loss of response at weeks 12, 30, and 52 and rate of mucosal healing and side effects at week 52. Results: Thirty-two children received ADA (median age 10 ± 4 years). Median disease duration before ADA therapy was 27 months. All patients received previous IFX (43% intolerant, 50% nonresponders [37.5% primary, 42.5% secondary nonresponders], 6.7% positive anti-IFX antibodies). Fifty-two weeks after ADA initiation, 13 patients (41%) were in corticosteroid-free remission. Mucosal healing occurred in 9 patients (28%) at 52 weeks. The cumulative probability of a clinical relapse-free course was 69%, 59%, and 53% at 12, 30, and 52 weeks, respectively. Ten patients (31%) had a primary failure and 5 (15%) a loss of response to ADA. No significant differences in efficacy were reported between not-responders and intolerant to IFX (P = 1.0). Overall, 19 patient (59%) maintained ADA during 52-week follow-up. Seven patients (22%) experienced an adverse event, no serious side effects were observed and none resulted in ADA discontinuation. Conclusions: Based on our data, ADA seems to be effective in children with UC, allowing to recover a significant percentage of patients intolerant or not-responding to IFX. The safety profile was good.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/294863

Assessment of mycotoxin exposure in breastfeeding mothers with celiac disease
NUTRIENTS
Autore/i: Valitutti, F.; De Santis, B.; Trovato, C. M.; Montuori, M.; Gatti, S.; Oliva, S.; Brera, C.; Catassi, C.
Classificazione: 1 Contributo su Rivista
Abstract: Objective: To assess the risk of mycotoxin exposure (aflatoxin M1, ochratoxin A, and zearalenone) in celiac disease (CD) breastfeeding mothers and healthy control mothers, as well as in their offspring, by quantifying these contaminants in breast milk. Study design: Thirty-five breastfeeding women with CD on a gluten-free diet and 30 healthy breastfeeding controls were recruited. Milk sampling was performed three times per day for three consecutive days. Mycotoxin content was investigated by an analytical method using immunoaffinity column clean-up and high-performance liquid chromatography (HPLC) with fluorometric detection. Results: Aflatoxin M1 (AFM1) was detected in 37% of CD group samples (mean ± SD = 0.012 ± 0.011 ng/mL; range = 0.003-0.340 ng/mL). The control group showed lower mean AFM1 concentration levels in 24% of the analyzed samples (0.009 ± 0.007 ng/mL; range = 0.003-0.067 ng/mL, ANOVA on ranks, p-value < 0.01). Ochratoxin A and zearalenone did not differ in both groups. Conclusion: Breast milk AFM1 contamination for both groups is lower than the European safety threshold. However, the estimated exposures of infants from CD mothers and control mothers was much higher (≃15 times and ≃11 times, respectively) than the threshold set by the joint FAO/WHO Expert Committee on Food Additives (JECFA). Since incongruities exist between JECFA and the European Union standard, a novel regulatory review of the available data on this topic is desirable. Protecting babies from a neglected risk of high AFM1 exposure requires prompt regulatory and food-control policies.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/294862

Comparison of Diagnostic Performance of the IgA Anti-tTG Test vs IgA AGA Test in Detection of Celiac Disease in the General Population
CLINICAL GASTROENTEROLOGY AND HEPATOLOGY
Autore/i: Verma, Anil K; Gatti, Simona; Lionetti, Elena; Galeazzi, Tiziana; Monachesi, Chiara; Franceschini, Elisa; Balanzoni, Linda; Scattolo, Novella; Cinquetti, Mauro; Catassi, Carlo
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/256981

Marcatori di danno intestinale nelle malattie infiammatorie croniche intestinali pediatriche
Autore/i: Gatti, Simona
Editore: Università Politecnica delle Marche
Classificazione: 8 Tesi di dottorato
Abstract: Background: The inflammatory bowel diseases (IBD) are chronic relapsing inflammatory disorders of the gastrointestinal tract, comprising Crohn’s disease (CD), ulcerative colitis (UC), and IBD-unclassified (IBD-U). The microbiome, the barrier function, and the immune system play an integrated role in the development of IBD, and all three components are likely critical for perpetuating the disease process. Aims and project: In this study we have preliminary investigated some specific aspects of the IBD pathogenesis including the intestinal dysbiosis, the alteration of the intestinal barrier and the effect of a specific dietetic treatment (the so-called exclusive enteral nutrition, EEN). Methods: IBD pediatric patients were enrolled in the period 2013-2015. The study included 3 type of analysis: 1. Microbiota study: analysis of fecal microbiota in IBD in general (through the amplification of the V3-V4 regions of the 16S rRNA gene); 2. EEN study: evaluation of the clinical, laboratory and microbiological changes induced by EEN; 3. IPT study: evaluation of the intestinal barrier damage in IBD patients by the intestinal permeability test (IPT). Results: preliminary results of the microbiota study showed a different prevalence in some specific phyla in CD patients compared to UC. Such changes were not correlated to the level of inflammation. The results of the EEN study support the efficacy of EEN in the treatment of CD and preliminary results show a shift in microbiota composition after a course of EEN. IPT was found to be a sensitive test to detect disease activity and to evaluate response to treatments. Conclusion: Our study has generated novel and intriguing data especially with regards to microbiota changes secondary to EEN and to the modification of intestinal permeability following specific treatments.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/245512

Microbiota profile in pediatric IBD: correlations with phenotype and disease activity
JOURNAL OF CROHN'S AND COLITIS
Autore/i: Gatti, S; Annibali, R; Del Baldo, G; Franceschini, E; Lionetti, E; Albano, V; Galeazzi, T; Catassi, C
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/294981

Pediatric chronic intestinal failure in italy: Report from the 2016 survey on behalf of Italian society for gastroenterology, hepatology and nutrition (SIGENP)
NUTRIENTS
Autore/i: Diamanti, Antonella; Capriati, Teresa; Gandullia, Paolo; Dileo, Grazia; Lezo, Antonella; Lacitignola, Laura; Spagnuolo, Mariaimmacolata; Gatti, Simona; D’Antiga, Lorenzo; Verlato, Giovanna; Roggero, Paola; Amarri, Sergio; Baldassarre, Mariaelisabetta; Cirillo, Francesco; Elia, Domenica; Boldrini, Renata; Campanozzi, Angelo; Catassi, Carlo; Aloi, Marina; Romano, Claudio; Candusso, Manila; Cecchi, Nicola; Bellini, Tommaso; Tyndall, Elaine; Fusaro, Fabio; Caldaro, Tamara; Alberti, Daniele; Gamba, Piergiorgio; Lima, Mario; Bagolan, Pietro; De Goyet, Jean De Ville; Dall’Oglio, Luigi; Spada, Marco; Grandi, Francesca
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/254941

Italian pediatric nutrition survey
CLINICAL NUTRITION ESPEN
Autore/i: Lezo, A.; Diamanti, A.; Capriati, T.; Gandullia, P.; Fiore, P.; Lacitignola, L.; Gatti, S.; Spagnuolo, M. I.; Cecchi, N.; Verlato, G.; Borodani, S.; Forchielli, L.; Panceri, R.; Brunori, E.; Pastore, M.; Amarri, S.; Abate, R.; Accorsi, P.; Aidala, E.; Amarri, S.; Ancora, G.; Angelotti, L.; Annibali, R.; Antonini Monica, A.; Banzato, C.; Barbaglia, M.; Bardasi, G.; Barrani, M.; Basso, T.; Brach del Prever, A.; Belli, F.; Bellomo Anna, R.; Besenzon, L.; Biagioni, M.; Bonaudo, R.; Bruni, G.; Cagnoli, G.; Campanozzi, A.; Casaccia, G.; Castello, M.; Chiaro, A.; Cimadore, N.; Cioni, M.; Cortinovis, F.; De Cosmi, V.; De Giacomo, C.; Del Vecchio, S.; Diamanti, A.; Di Leo, G.; Di Scala, C.; Famiani, A.; Felici, E.; Ferraris, S.; Fomasi, M.; Fontanella, G.; Frimaire, A.; Fusco, P.; Galvagno, G.; Gandullia, P.; Gasparrini, E.; Guana, R.; Guerra, A.; Lanari, M.; Lacitignola, L.; Lizzoli, F.; Maggiore, G.; Magista Anna, M.; Malaventura, C.; Marmetucci, L.; Mazzocchi, A.; Mazzoni, E.; Melli, P.; Memmini, G.; Meneghini, A.; Miglietti, N.; Migliore, G.; Mistura, L.; Monaci, A.; Morganti, A.; Nesi, F.; Opinto, V.; Pace, M.; Palamone, G.; Panceri, R.; Parisi, G.; Pastore, M.; Penagini, F.; Perrone, M.; Petitti, P.; Pettinari, C.; Peverelli, P.; Pinon, M.; Russo, C.; Sala, A.; Salata, M.; Salmaso, M.; Sangerardi, M.; Santangelo, B.; Savino, F.; Scata, D.; Siani, P.; Spagnuolo, M. I.; Sparano, P.; Stamati Filomena, A.; Tulli, M.; Uga, E.; Urbano, F.; Zoppo, M.; Zuin, G.
Classificazione: 1 Contributo su Rivista
Abstract: Introduction the prevalence of malnutrition in children and its impact on clinical outcomes is underrecognized by clinicians in Italy as well as worldwide. A novel definition of pediatric malnutrition has been recently proposed by a working group of the Academy of Nutrition and Dietetics and American Society for Parenteral and Enteral Nutrition (A.S.P.E.N.), based on the correlation between illness and the use of zscores of anthropometric measurements. Aim to investigate the prevalence of malnutrition and related nutritional support among hospitalized children in Italy, in a nationwide survey performed in a single day (16/4/2015). Methods an open access website (http://nday.biomedia.net) was used to collected data from 73 hospitals and 101 wards in 14 Italian regions (1994 patients). Anonymous information was collected on hospitals' characteristics, patient's anthropometry, admission diagnosis, presence of chronic diseases and use of nutritional support: oral nutritional supplements (ONS), enteral nutrition (EN) or parenteral nutrition (PN). Z-scores of anthropometric measurements, calculated with Epi Info 7.1.5, defined nutritional status: wasting was identified by BMI or Weight-for-Length z-score (<−1 mild, <−2 moderate, <−3 severe), stunting by Height-for-Age Z-score <−2. WHO 2006 and CDC 2000 growth charts were used respectively for children younger and older than 2 years old. Results 1790 complete records were obtained for hospitalized patients aged 0–20 years, with median age 6.16 (0.1–20 years and 53.3% males). 52.9% were aged 0–6 years and 58.8% of children suffered from chronic diseases. Wasting was detected in 28.7% of the total sample with higher occurrence observed in age ranges 0–6 and 14–20 years, while 17.3% of patients showed stunting; surprisingly almost 27% of them were aged 0–2. A ranking of the admission diagnosis with the highest rate of malnutrition was complied. The prevalence of wasting was significantly (p < 0.005) higher amongst children with chronic diseases (34.1% vs. 27.1%); stunting prevalence tripled in patients with chronic disease (24.5% vs. 8.3%). Only 23.5% of malnourished children (17%, 25.6% and 36.7%, respectively mild, moderate and severe malnutrition) received nutritional support: 11.7% received oral nutrition supplements (ONS, modular or complete), 11.5% enteral nutrition (EN, 6.4% via nasogastric tube, 5.1% via gastrostomy) and 6.8 % received parenteral nutrition (PN); in some patients a combination of two. Nutritional support is more commonly used among stunting patients, 39.5% of children under treatment. Conclusion Malnutrition of any grade was observed in nearly 1/3 and stunting in 17% of the reported hospitalized children, and it is likely to be underrecognized as the nutritional support reached only a small part of the malnourished children.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/294861

The low FODMAP diet: Many question marks for a catchy acronym
NUTRIENTS
Autore/i: Catassi, Giulia; Lionetti, MARIA ELENA; Gatti, Simona; Catassi, Carlo
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/249689

Quality of life reported by Italian children with IBD and their parents: Preliminary results of a multicenter SIGENP study
DIGESTIVE AND LIVER DISEASE
Autore/i: Gatti, S; Del Baldo, G; Aloi, M; Bramuzzo, M; Penagini, F; Pastore, M; Panceri, R; Ottaviano, G; Franceschini, E; Martelossi, S; Cucchiara, S; Catassi, C
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/294982

Gluten contamination in naturally or labeled gluten-free products marketed in Italy
NUTRIENTS
Autore/i: Verma, ANIL KUMAR; Gatti, Simona; Galeazzi, Tiziana; Monachesi, Chiara; Padella, Lucia; DEL BALDO, Giada; Annibali, Roberta; Lionetti, MARIA ELENA; Catassi, Carlo
Classificazione: 1 Contributo su Rivista
Abstract: ACKGROUND: A strict and lifelong gluten-free diet is the only treatment of celiac disease. Gluten contamination has been frequently reported in nominally gluten-free products. The aim of this study was to test the level of gluten contamination in gluten-free products currently available in the Italian market. METHOD: A total of 200 commercially available gluten-free products (including both naturally and certified gluten-free products) were randomly collected from different Italian supermarkets. The gluten content was determined by the R5 ELISA Kit approved by EU regulations. RESULTS: Gluten level was lower than 10 part per million (ppm) in 173 products (86.5%), between 10 and 20 ppm in 9 (4.5%), and higher than 20 ppm in 18 (9%), respectively. In contaminated foodstuff (gluten > 20 ppm) the amount of gluten was almost exclusively in the range of a very low gluten content. Contaminated products most commonly belonged to oats-, buckwheat-, and lentils-based items. Certified and higher cost gluten-free products were less commonly contaminated by gluten. CONCLUSION: Gluten contamination in either naturally or labeled gluten-free products marketed in Italy is nowadays uncommon and usually mild on a quantitative basis. A program of systematic sampling of gluten-free food is needed to promptly disclose at-risk products.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/245860

The low FODMAP diet: Many question marks for a catchy acronym
NUTRIENTS
Autore/i: Catassi, G.; Lionetti, E.; Gatti, S.; Catassi, C.
Classificazione: 1 Contributo su Rivista
Abstract: FODMAP, “Fermentable Oligo-, Di- and Mono-saccharides And Polyols”, is a heterogeneous group of highly fermentable but poorly absorbed short-chain carbohydrates and polyols. Dietary FODMAPs might exacerbate intestinal symptoms by increasing small intestinal water volume, colonic gas production, and intestinal motility. In recent years the low-FODMAP diet for treatment of irritable bowel syndrome (IBS) has gained increasing popularity. In the present review we aim to summarize the physiological, clinical, and nutritional issues, suggesting caution in the prolonged use of this dietary treatment on the basis of the existing literature. The criteria for inclusion in the FODMAPs list are not fully defined. Although the low-FODMAP diet can have a positive impact on the symptoms of IBS, particularly bloating and diarrhea, the quality of the evidence is lower than optimal, due to frequent methodological flaws, particularly lack of a proper control group and/or lack of blinding. In particular, it remains to be proven whether this regimen is superior to conventional IBS diets. The drastic reduction of FODMAP intake has physiological consequences, e.g., on the intestinal microbiome and colonocyte metabolism, which are still poorly understood. A low-FODMAP diet imposes an important restriction of dietary choices due to the elimination of some staple foods, such as wheat derivatives, lactose-containing dairy products, many vegetables and pulses, and several types of fruits. For this reason, patients may be at risk of reduced intake of fiber, calcium, iron, zinc, folate, B and D vitamins, and natural antioxidants. The nutritional risk of the low-FODMAP diet may be higher in persons with limited access to the expensive, alternative dietary items included in the low-FODMAP diet.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/294918

Research gaps in diet and nutrition in inflammatory bowel disease. A topical review by D-ECCO working group [Dietitians of ECCO]
JOURNAL OF CROHN'S AND COLITIS
Autore/i: Sigall-Boneh, R.; Levine, A.; Lomer, M.; Wierdsma, N.; Allan, P.; Fiorino, G.; Gatti, S.; Jonkers, D.; Kierkus, J.; Katsanos, K. H.; Melgar, S.; Yuksel, E. S.; Whelan, K.; Wine, E.; Gerasimidis, K.
Classificazione: 1 Contributo su Rivista
Abstract: Although the current doctrine of IBD pathogenesis proposes an interaction between environmental factors and gut microbiota in genetically susceptible individuals, dietary exposures have attracted recent interest and are, at least in part, likely to explain the rapid rise in disease incidence and prevalence. The D-ECCO working group along with other ECCO experts with expertise in nutrition, microbiology, physiology, and medicine reviewed the evidence investigating the role of diet and nutritional therapy in the onset, perpetuation, and management of IBD. A narrative topical review is presented where evidence pertinent to the topic is summarised collectively under three main thematic domains: i] the role of diet as an environmental factor in IBD aetiology; ii] the role of diet as induction and maintenance therapy in IBD; and iii] assessment of nutritional status and supportive nutritional therapy in IBD. A summary of research gaps for each of these thematic domains is proposed, which is anticipated to be agenda-setting for future research in the area of diet and nutrition in IBD.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/294864

Re-exploring the iceberg of celiac disease in children: Results of a multicenter Italian screening project, based on a rapid HLA DQ typing test
DIGESTIVE AND LIVER DISEASE
Autore/i: Gatti, S; Galeazzi, T; Verma, Ak; Franceschini, E; Annibali, R; Del Baldo, G; Palpacelli, A; Marchesini, A; Monachesi, C; Balanzoni, L; Colombari, A; Scattolo, N; Trevisan, M; Cinquetti, M; Lionetti, E; Catassi, C
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/294983

Re-challenge studies in non-celiac gluten sensitivity: A systematic review and meta-analysis
FRONTIERS IN PHYSIOLOGY
Autore/i: Lionetti, Elena; Pulvirenti, Alfredo; Vallorani, Martina; Catassi, Giulia; Verma, Anil K.; Gatti, Simona; Catassi, Carlo
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/254636

Effects of the exclusive enteral nutrition on the microbiota profile of patients with crohn’s disease: A systematic review
NUTRIENTS
Autore/i: Gatti, Simona; Galeazzi, Tiziana; Franceschini, Elisa; Annibali, Roberta; Albano, Veronica; Verma, Anil Kumar; DE ANGELIS, MARIA VITTORIA; Lionetti, MARIA ELENA; Catassi, Carlo
Classificazione: 1 Contributo su Rivista
Abstract: The mechanisms behind the efficacy of exclusive enteral nutrition (EEN) in Crohn's disease (CD) remain poorly understood, despite the high rate of treatment response. Evidence accumulated in the last 20 years suggests that a positive shift of the disrupted microbiota is one of the treatment effects. The purpose of this study was to critically review and summarize data reporting the microbiological effects of EEN in patients with CD. Fourteen studies were considered in the review, overall involving 216 CD patients on EEN. The studies were heterogeneous in methods of microbiota analysis and exclusion criteria. The most frequently reported effect of EEN was a reduction in microbiota diversity, reversible when patients returned to a normal diet. The effect of EEN on specific bacteria was very variable in the different studies, partially due to methodological limitations of the mentioned studies. The EEN seem to induce some metabolomic changes, which are different in long-term responder patients compared to patients that relapse earlier. Bacterial changes can be relevant to explaining the efficacy of EEN; however, microbiological data obtained from rigorously performed studies and derived from last generation techniques are largely inconsistent.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/253473

IMERSLUND-GRASBECK SYNDROME (SELECTIVE B12 MALABSORPTION): THINK ABOUT IT ALSO IN DOWN'S SYNDROME!
DIGESTIVE AND LIVER DISEASE
Autore/i: Del Baldo, G; Marabini, C; Albano, V; Lionetti, Me; Gatti, S
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/294987

Efficacy of intravenous immunoglobulin therapy in giant cell hepatitis with autoimmune hemolytic anemia: A multicenter study
CLINICS AND RESEARCH IN HEPATOLOGY AND GASTROENTEROLOGY
Autore/i: Marsalli, G.; Nastasio, S.; Sciveres, M.; Calvo, P. L.; Ramenghi, U.; Gatti, S.; Albano, V.; Lega, S.; Ventura, A.; Maggiore, G.
Classificazione: 1 Contributo su Rivista
Abstract: Background and objective: Giant cell hepatitis with autoimmune hemolytic anemia (GCH-AHA) is a rare disease of infancy, of possible autoimmune mechanism with poor prognosis due to its scarce response to immunosuppressive drugs. The aim of this retrospective multicenter study was to evaluate the efficacy and safety of intravenous immunoglobulin (IVIg) treatment in inducing and maintaining remission of the liver disease, in patients with GCH-AHA. Methods: Seven children with GCH-AHA, four newly diagnosed, and three in relapse, being treated with different therapies, received one to three IVIg infusions (0.5 to 2 g/kg) in association with other immunosuppressive drugs. Subsequently five of them received monthly sequential IVIg infusions (mean 13.4, range 7-24). Results: IVIg infusions as first-line therapy associated with prednisone and other immunosuppressive drugs significantly (P = 0.04) reduced the aminotransferase activity in all patients and normalized prothombin activity in the only patient with severe liver dysfunction. Sequential monthly IVIg infusions determined a steroid-sparing effect and allowed a complete or partial remission in all patients, although with temporary efficacy, since relapse of the hemolytic anemia and/or of liver disease occurred in all patients. IVIg infusions were associated with mild side effects in two patients. Conclusions: IVIg infusion can be safely and effectively administered in patients with severe GCH-AHA at diagnosis, or in case of relapse, in association with other immunosuppressive drugs. Repeated IVIg infusions may help maintain remission, however, due to their temporary efficacy, they should not be routinely employed.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/294866

OATS IN THE DIET OF CHILDREN WITH CELIAC DISEASE: A DOUBLE-BLIND, RANDOMIZED, PLACEBO-CONTROLLED MULTICENTER STUDY
DIGESTIVE AND LIVER DISEASE
Autore/i: Lionetti, E; Gatti, S; Caporelli, N; Galeazzi, T; Francavilla, R; Barbato, M; Roggero, P; Malamisura, B; Iacono, G; Tomarchio, S; Budelli, A; Gesuita, R; Catassi, C
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/294986

NUTRITION IN INFANTS UNDERGOING SURGERY FOR CONGENITAL HEART DISEASE. A LONGITUDINAL STUDY IN 71 CHILDREN
DIGESTIVE AND LIVER DISEASE
Autore/i: Caporelli, N; Capestro, A; Gatti, S; Baldinelli, A; Lionetti, Me; Catassi, C; Pozzi, M
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/294984

PAY ATTENTION TO NEONATAL JAUNDICE
DIGESTIVE AND LIVER DISEASE. SUPPLEMENT
Autore/i: Spagnoli, C; Albano, V; Cobellis, G; Lionetti, E; Gatti, S; Catassi, Carlo
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/293468

EFFICACY AND SAFETY OF ADALIMUMAB AFTER INFLIXIMAB FAILURE IN PEDIATRIC ULCERATIVE COLITIS
DIGESTIVE AND LIVER DISEASE
Autore/i: Aloi, M; Bramuzzo, M; Arrigo, S; Romano, C; Iacorte, D; Gatti, S; Illiceto, Mt; Zucconi, F; Dilillo, D; Zuin, G; Knafelz, D; Ravelli, A; Viola, F; Alvisi, P
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/294980

DETECTION OF GLUTEN CONTENT IN THE NATURALLY GLUTEN FREE AND GLUTEN FREE LABELLED COMMERCIALLY AVAILABLE FOOD PRODUCTS IN ITALY
DIGESTIVE AND LIVER DISEASE
Autore/i: Verma, Ak; Gatti, S; Galeazzi, T; Monachesi, C; Padella, L; Del, Bg; Annibali, R; Lionetti, E; Catassi, C
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/294985

Effect of Early Versus Late Azathioprine Therapy in Pediatric Ulcerative Colitis
INFLAMMATORY BOWEL DISEASES
Autore/i: Aloi, M.; D'Arcangelo, G.; Bramuzzo, M.; Gasparetto, M.; Martinelli, M.; Alvisi, P.; Illiceto, M. T.; Valenti, S.; Distante, M.; Pellegrino, S.; Gatti, S.; Arrigo, S.; Civitelli, F.; Martelossi, S.
Classificazione: 1 Contributo su Rivista
Abstract: Background: We aimed at describing the efficacy of azathioprine (AZA) in pediatric ulcerative colitis, comparing the outcomes of early (0-6 months) versus late (6-24 months) initiation of therapy. Methods: Children with ulcerative colitis treated with AZA within 24 months of diagnosis were included. Corticosteroid (CS)-free remission and mucosal healing (MH), assessed by endoscopy or fecal calprotectin, at 12 months were the primary outcomes. Patients were also compared for CS-free remission and MH, need for treatment escalation or surgery, number of hospitalizations, and adverse events during a 24-month follow-up. Results: A total of 121 children entered the study (median age 10.5 ± 4.0 years, 59% girls). Seventy-six (63%) started AZA between 0 and 6 months (early group) and 45 (37%) started between 6 and 24 months (late group). Seventy-five percent and 53% of patients in the early and late group, respectively, received CS at the diagnosis (P 0.01). CS-free remission at 1 year was achieved by 30 (50%) of the early and 23 (57%) of the late patients (P 0.54). MH occurred in 37 (37%) patients at 1 year, with no difference between the 2 groups (33% early, 42% late; P 0.56). No difference was found for the other outcomes. Conclusions: Introduction of AZA within 6 months of diagnosis seems not more effective than later treatment to achieve CS-free remission in pediatric ulcerative colitis. MH does not depend on the timing of AZA initiation; however, because of the incomplete comparability of the 2 groups at the diagnosis and the use of fecal calprotectin as a surrogate marker of MH, our results should be further confirmed by prospective studies.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/294865

World perspective and celiac disease epidemiology
DIGESTIVE DISEASES
Autore/i: Catassi, Carlo; Gatti, S; Lionetti, MARIA ELENA
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/228787

DOES CELIAC SERO-DIAGNOSIS ALWAYS REQUIRE A TERTIARY REFERRAL CENTRE CONFIRMATION? OUR EXPERIENCE IN MARCHE REGION
DIGESTIVE AND LIVER DISEASE. SUPPLEMENT
Autore/i: Del Baldo, G; Franceschini, E; Rossi, M; Albano, V; Cobellis, G; Gatti, S; Catassi, C
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/293471

Quality of life in children with celiac disease: A paediatric cross-sectional study
DIGESTIVE AND LIVER DISEASE
Autore/i: Biagetti, Chiara; Gesuita, Rosaria; Gatti, Simona; Catassi, Carlo
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/228791

Quality of life in children with celiac disease: A paediatric cross-sectional study
DIGESTIVE AND LIVER DISEASE
Autore/i: Biagetti, C.; Gesuita, R.; Gatti, S.; Catassi, C.
Classificazione: 1 Contributo su Rivista
Abstract: Background: Few studies investigated factors influencing the quality of life of children with celiac disease on a gluten-free diet. Aims: To investigate the impact of the gluten-free diet on the psycho-physical well-being of celiac children. Methods: In this cross-sectional study, we interviewed 76 celiac and 143 non-celiac children (2-18 years) by using a non-disease specific questionnaire (Pediatric Quality of Life Inventory Test) and we explored the impact of the diet on social life with an open-ended questionnaire. Scores were compared by Wilcoxon rank-sum test. A quantile regression analysis was used to evaluate the impact of celiac disease on score distribution. Results: No significant differences in quality of life were found between the two groups (total score: 84.1 (81.1-87.2) vs 81.5 (79.7-83.4), median (95% CI), patients and controls respectively, p=0.4). Treatment positively affected quality of life in children that showed "intermediate" scores in the Pediatric Quality of Life Inventory Test. Lowest scores were observed in children reporting a higher number of diet difficulties or co-morbidities. Conclusions: Although celiac patients showed an overall good quality of life in comparison with a control group, by using appropriate analytical methods we elicited specific factors contributing to a lower quality of life in patients, such as co-morbidities and difficulties with the diet.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/294917

Celiac disease from a global perspective
BAILLIERE'S BEST PRACTICE & RESEARCH. CLINICAL GASTROENTEROLOGY
Autore/i: Lionetti, MARIA ELENA; Gatti, S; Pulvirenti, A; Catassi, Carlo
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/228788

CROHN'S DISEASE AND EARLY ISOLATED ORAL PRESENTATION
DIGESTIVE AND LIVER DISEASE. SUPPLEMENT
Autore/i: Coppari, C; Rossi, M; Gatti, S; Albano, V; Mandolesi, A; Cobellis, G; Catassi, C
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/293469

EBV AND CMV STATUS IN CHILDREN WITH IBD AT DIAGNOSIS: A CASE CONTROL STUDY
DIGESTIVE AND LIVER DISEASE
Autore/i: Annibali, R; Gatti, S; Coccia, P; Pierani, P; Catassi, C
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/294989

Celiac disease from a global perspective
BAILLIERE'S BEST PRACTICE & RESEARCH. CLINICAL GASTROENTEROLOGY
Autore/i: Lionetti, E.; Gatti, S.; Pulvirenti, A.; Catassi, C.
Classificazione: 1 Contributo su Rivista
Abstract: Celiac disease (CD) is one of the commonest lifelong disorders in countries populated by individuals of European origin, affecting approximately 1% of the general population. This is a common disease also in North Africa, Middle East and India. The widespread diffusion of CD is not surprising given that its causal factors (HLA predisposing genotypes and consumption of gluten-containing cereals) show a worldwide distribution. Further studies are needed to quantify the incidence of CD in apparently "celiac-free" areas such as Sub-Saharan Africa and Far East. Several reports have shown that CD is increasing in frequency in different geographic areas. Genetic factors do not explain the rising incidence during the last decades; environmental or lifestyle factors may be responsible for these changes over time. The majority of patients with CD are still undiagnosed all over the world, leading to debate about the need of screening program.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/294916

How much nutrition matters for children
RECENTI PROGRESSI IN MEDICINA
Autore/i: Catassi, Carlo; Gatti, Simona
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/223918

SERUM MICRONUTRIENTS ASSESSMENT IN CHILDREN WITH SHORT BOWEL SYNDROME
DIGESTIVE AND LIVER DISEASE
Autore/i: Annibali, R; Rossi, M; Santini, L; Gatti, S; Spagnoli, C; Coppari, C; Albano, V; Catassi, C
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/294991

World perspective and celiac disease epidemiology
DIGESTIVE DISEASES
Autore/i: Catassi, C.; Gatti, S.; Lionetti, E.
Classificazione: 1 Contributo su Rivista
Abstract: In Europe and the USA, the mean frequency of celiac disease (CD) in the general population is approximately 1%, with some regional differences, the reasons for which remain elusive. A similar disease prevalence has been found in other countries mostly populated by individuals of European origin, e.g. Australia and Argentina. In Western countries, a true rise in overall CD prevalence of CD has been documented. CD is a common disorder in North Africa, the Middle East and India; however, the diagnostic rate is low in these countries due to low availability of diagnostic facilities and poor disease awareness. The highest CD prevalence in the world (5.6%) has been described in an African population originally living in Western Sahara, the Saharawi, of Arab-Berber origin. The reasons for this high CD frequency are unclear but could be primarily related to recent dietary changes and genetic factors, given the high level of consanguinity of this population. Further studies are needed to quantify the incidence of the celiac condition in apparently 'celiac-free' areas such as Sub-Saharan Africa and the Far East. In many developing countries, the frequency of CD is likely to increase in the near future given the diffuse tendency to adopt Western, gluten-rich dietary patterns. As most cases currently escape diagnosis all over the world, an effort should be made to increase the awareness of CD polymorphism. A cost-effective case-finding policy and new strategies of mass CD screening could significantly reduce the morbidity and mortality associated with untreated disease. The current high prevalence of CD is just the last link in a chain of events started about 10,000 years ago after wheat domestication and diffusion from the Middle East. We hypothesize different mechanisms to explain the so-called evolutionary celiac paradox of co-localization of gluten consumption and HLA CD-predisposing genotypes.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/294915

Assessing quality of care in paediatric inflammatory bowel disease: Focusing on self-reported outcomes
DIGESTIVE AND LIVER DISEASE
Autore/i: Gatti, S.; Brennan, M.; Heuschkel, R.; Zilbauer, M.
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/294867

Beyond the intestinal mucosa: diagnostic role of anti-TG2 deposits, a systematic review.
FRONTIERS IN MEDICINE
Autore/i: Gatti, Simona; Rossi, M; Alfonsi, S; Mandolesi, A; Cobellis, Giovanni; Catassi, Carlo
Classificazione: 1 Contributo su Rivista
Abstract: To review the existing literature on the role and significance of intestinal transglutaminase 2 immunoglobulin A deposits (TG2 deposits) in patients with overt celiac disease (CD), potential celiac disease (PCD), and other autoimmune or gluten-related conditions. We conducted a systematic review of studies published in English, evaluating presence and characteristics of TG2 deposits in subjects with overt CD, PCD, gluten-related diseases [dermatitis herpetiformis (DH), gluten-ataxia (GA)], autoimmune disorders (type-1 diabetes), and other conditions. Studies were identified through a MEDLINE search (1950-2013). Twenty-three studies were included in the review. Eleven studies were performed in children. Overall TG2 deposits were present in 100% of adults with overt CD, while in children prevalence ranged from 73.2 to 100%. Six studies with an established definition of PCD were considered, prevalence of deposits ranging from 64.7 to 100%. A single study followed-up PCD patients with repeated biopsies and identified presence of intestinal deposits as the best marker to reveal progression toward villous atrophy. Two studies investigated presence of deposits in DH, reporting prevalence between 63 and 79%. A single study documented TG2 deposits in 100% of patients with GA. In children with type-1 diabetes (T1D), positivity of intestinal TG2 deposits ranged from 25 to 78%. Transglutaminase 2 IgA deposits seem to be a constant feature in overt CD patients and are frequently detectable in other gluten-related conditions (DH and GA). The vast majority of PCD patients express TG2 deposits at the intestinal level, but no sufficient data are available to exactly define their prognostic role as a marker of evolution toward overt CD. The frequent finding of TG2 deposits in the intestinal mucosa of patients with T1D is an interesting observation deserving further evaluation. Beyond the Intestinal Celiac Mucosa: Diagnostic Role of Anti-TG2 Deposits, a Systematic Review (PDF Download Available). Available from: http://www.researchgate.net/publication/272750428_Beyond_the_Intestinal_Celiac_Mucosa_Diagnostic_Role_of_Anti-TG2_Deposits_a_Systematic_Review [accessed Dec 6, 2015].
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/223917

Celiac disease: past, present and future challenges: dedicated to the memory of our friend and colleague, Prof David Branski (1944-2013)
JOURNAL OF PEDIATRIC GASTROENTEROLOGY AND NUTRITION
Autore/i: Shamir, R; Heyman, Mb; Koning, F; Wijimenga, C; Gutierrez Achury, J; Catassi, Carlo; Gatti, Simona; Fasano, A; Discepolo, V; Korponay Szabo, Ir; Zevit, N; Maki, M; Schaart, Mw; Mearin, Ml; Troncone, R.
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/223741

A case of dumping syndrome in a patient undergoing esophageal atresia surgery
DIGESTIVE AND LIVER DISEASE. SUPPLEMENT
Autore/i: Rossi, Matilde; Annibali, R; Tonelli, Lisa; Palpacelli, Alessandra; Albano, V; Gatti, S; Cobellis, G; Catassi, C.
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/234489

Anti glycoprotein-2 antibody in pediatric inflammatory bowel disease and celiac disease: prevalence, diagnostic value and variation at follow-up
JOURNAL OF CROHN'S AND COLITIS
Autore/i: Vallorani, M; Gatti, S; Romagnoli, V; Marinelli, L; Ciarrocchi, G; Catassi, C
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/294988

The new epidemiology of celiac disease
JOURNAL OF PEDIATRIC GASTROENTEROLOGY AND NUTRITION
Autore/i: Catassi, Carlo; Gatti, Simona; Fasano, A.
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/223742

Short bowel disease in infants: State of art in our center
DIGESTIVE AND LIVER DISEASE. SUPPLEMENT
Autore/i: Gatti, S; Annibali, R; Rossi, M; Albano, V; Cobellis, Giovanni; Catassi, C.
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/234487

Oats in the diet of children with celiac disease: preliminary results of a double-blind, randomized, placebo-controlled multicenter Italian study.
NUTRIENTS
Autore/i: Gatti, Simona; Caporelli, N; Galeazzi, Tiziana; Francavilla, R; Barbato, M; Roggero, P; Malamisura, B; Iacono, G; Budelli, A; Gesuita, Rosaria; Catassi, Carlo; Lionetti, MARIA ELENA
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/161311

The novel crohn's disease marker anti-GP2 antibody is associated with ileocolonic location of disease
GASTROENTEROLOGY RESEARCH AND PRACTICE
Autore/i: Somma, V.; Ababneh, H.; Ababneh, A.; Gatti, S.; Romagnoli, V.; Bendia, E.; Conrad, K.; Bogdanos, D. P.; Roggenbuck, D.; Ciarrocchi, G.
Classificazione: 1 Contributo su Rivista
Abstract: Crohn's disease (CD) is an inflammatory bowel disease (IBD) that can affect the whole gastrointestinal tract. The ileocolonic variant of CD, an inflammation of both the ileum and the large intestine, accounts for up to 50% of the cases with CD, whereas Crohn's ileitis affecting the ileum is diagnosed in about 30%. Crohn's colitis, which is confined to the large intestine and accounts for the remaining 20%, is difficult to distinguish from the large bowel inflammation seen in patients with ulcerative colitis (UC). The pathogenesis of CD is not yet completely understood. Autoimmunity is one factor that can partake in the triggering or modulation of inflammatory processes in IBD. The major zymogen-granule membrane glycoprotein 2 (GP2) has been recently identified as a major autoantigenic target in CD. Interestingly, GP2 is mainly expressed in the pancreas and has also been demonstrated to be a membrane-anchored receptor of microfold cells in the follicle-associated epithelium. Remarkably, GP2 is overexpressed at the site of CD inflammation in contrast to the one in UC. By utilizing novel enzyme-linked immunosorbent assays for the detection of GP2-specific IgA and IgG, the loss of tolerance to GP2 has been associated with a specific clinical phenotype in CD, in particular with the ileocolonic location of the disease. © 2013 Valentina Somma et al.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/294868

Abdominal distension in an infant
GASTROENTEROLOGY
Autore/i: De Benedictis, F. M.; Gatti, S.
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/294869

A case of syndromic neutropenia and mutation in G6PC3
JOURNAL OF PEDIATRIC HEMATOLOGY ONCOLOGY
Autore/i: Gatti, S.; Boztug, K.; Pedini, A.; Pasqualini, C.; Albano, V.; Klein, C.; Pierani, P.
Classificazione: 1 Contributo su Rivista
Abstract: Background: A previously unrecognized syndrome with congenital neutropenia and various organ abnormalities has been described recently, caused by mutations in the gene encoding glucose-6-phosphatase, catalytic subunit 3 (G6PC3). Observation: A 10-year-old boy from Ecuador suffering from severe neutropenia and multiple nonhematopoietic abnormalities was admitted to our department. We identified a novel mutation in the G6PC3 gene (c. 765-delAG; p.S255fs). Conclusions: This is the first case of G6PC3 deficiency in a patient from South America, caused by a novel mutation in the G6PC3 gene. Our results give insights into the molecular and clinical variability of this disease. Copyright © 2011 by Lippincott Williams & Wilkins.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/294870

SCREENING OF CELIAC DISEASE IN LIBYAN SCHOOLCHILDREN AND DIAGNOSTIC ACCURACY OF A RAPID COMMERCIAL ASSAY FOR ANTI-TRANSGLUTAMINASE ANTIBODIES DETERMINATION
DIGESTIVE AND LIVER DISEASE
Autore/i: Gatti, S; Biagetti, C; Giorgetti, C; Harown, J; Alarida, K; Catassi, C
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/294990