116 pubblicazioni classificate nel seguente modo:

Assessment of molecularly imprinted polymers for selective removal of diclofenac from wastewater by laboratory and pilot-scale adsorption tests
JOURNAL OF WATER PROCESS ENGINEERING
Autore/i: Parlapiano, Marco; Foglia, Alessia; Sgroi, Massimiliano; Pisani, Michela; Astolfi, Paola; Mezzelani, Marica; Gorbi, Stefania; Akyol, Çağrı; Eusebi, Anna Laura; Fatone, Francesco
Classificazione: 1 Contributo su Rivista
Abstract: This study investigated the potential of Molecularly Imprinted Polymers (MIPs) to integrate schemes for wastewater reuse and to serve as effective adsorbent for the removal of a target emerging contaminant (i.e., diclofenac – DCF), after or within pilot scale anaerobic biological treatments. Batch tests were performed to evaluate the effect on DCF removal during anaerobic biological processes by enriching activated sludge of an Upflow Anaerobic Sludge Blanket (UASB) reactor (TSS =19.6 g/L), and an Anaerobic Membrane Bioreactor (AnMBR) (TSS = 120 mg/L) reactors with MIPs (3 mg/L). Therefore, tertiary treatments were investigated by columns adsorption tests that were performed first at lab scale using DCF (15 mg/L) solution in deionized water, and then in-site by treating the anaerobic permeate effluent from the AnMBR at pilot scale level (DCF 500 μg/L). Clogging or blockage of the column bed was not observed during these field tests, where the saturation process of MIPs was slower compared to laboratory tests that used deionized water. In addition, the empirical Thomas Model, Yoon-Nelson Model, Dose-Response Model and Adam Bohart Model showed very good fittings with the experimental data obtained during experiments performed with both synthetic water and anaerobic effluents showing their suitability for the description of breakthrough curves. Finally, it was observed that after regeneration the MIPs can be efficiently reused since adsorption capacity is sufficiently preserved.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/329513

First In Vivo Insights on the Effects of Tempol-Methoxycinnamate, a New UV Filter, as Alternative to Octyl Methoxycinnamate, on Zebrafish Early Development
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Autore/i: Damiani, Elisabetta; Sella, Fiorenza; Astolfi, Paola; Galeazzi, Roberta; Carnevali, Oliana; Maradonna, Francesca
Classificazione: 1 Contributo su Rivista
Abstract: The demand for organic UV filters as active components in sunscreen products has rapidly risen over the last century, as people have gradually realized the hazards of overexposure to UV radiation. Their extensive usage has resulted in their ubiquitous presence in different aquatic matrices, representing a potential threat to living organisms. In this context, the need to replace classic UV filters such as octyl methoxycinnamate (OMC), one of the most popular UV filters reported to be a potential pollutant of aquatic ecosystems, with more environmentally friendly ones has emerged. In this study, using zebrafish, the first in vivo results regarding the effect of exposure to tempol-methoxycinnamate (TMC), a derivative of OMC, are reported. A comparative study between TMC and OMC was performed, analyzing embryos exposed to similar TMC and OMC concentrations, focusing on morphological and molecular changes. While both compounds seemed not to affect hatching and embryogenesis, OMC exposure caused an increase in endoplasmic reticulum (ER) stress response genes, according to increased eif2ak3, ddit3, nrf2, and nkap mRNA levels and in oxidative stress genes, as observed from modulation of the sod1, sod2, gpr, and trx mRNA levels. On the contrary, exposure to TMC led to reduced toxicity, probably due to the presence of the nitroxide group in the compound's molecular structure responsible for antioxidant activity. In addition, both UV filters were docked with estrogen and androgen receptors where they acted differently, in agreement with the molecular analysis that showed a hormone-like activity for OMC but not for TMC. Overall, the results indicate the suitability of TMC as an alternative, environmentally safer UV filter.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/318432

The influence of mannose-based esters on the mesophase behaviour of lyotropic liquid crystalline nanosystems as drug delivery vectors
COLLOIDS AND SURFACES. B, BIOINTERFACES
Autore/i: Tiboni, M.; Astolfi, P.; Verboni, M.; Benedetti, S.; Giorgini, E.; Notarstefano, V.; Vita, F.; Ranieri, S.; Duranti, A.; Lucarini, S.; Casettari, L.; Pisani, M.
Classificazione: 1 Contributo su Rivista
Abstract: Lyotropic Liquid Crystalline (LLC) nanoparticles represent an emerging class of smart, biocompatible, and biodegradable systems for the delivery of drugs. Among these, structures with complex 3D architectures such as cubosomes are of particular interest. These are non- lamellar assemblies having hydrophobic and hydrophilic portions able to carry drugs of different nature. They can further be modulated including suitable additives to control the release of the active payload, and to promote an active targeting. Starting from monoolein (GMO) cubic phase, different concentrations of mannose-based esters were added, and the eventual structural modifications were monitored to ascertain the effects of the presence of glycolipids. Moreover, the structural properties of these nanosystems loaded with Dexamethasone (DEX), a very well-known anti-inflammatory steroid, were also studied. Experiments were carried out by synchrotron Small Angle X-ray Scattering (SAXS), Raman Microspectroscopy (RMS) and Attenuated Total Reflectance-Fourier Transform Infrared (ATR-FTIR) measurements. The drug delivery potential (i.e. entrapment efficiency and release properties) of the obtained nanoparticles was evaluated. Finally, in vitro cytocompatibility and anti-inflammatory activity studies of the prepared formulations were carried out. Inclusion of mannose-based surfactants up to 10 mol% influenced the structural parameters of Im3m cubic phase and swollen cubic phases were obtained with the different glycolipids with lattice parameters significantly higher than GMO. A complete cytocompatibility and an increased DEX activity were observed, thus suggesting the possibility to use GMO/glycolipids nanoparticles to formulate innovative drug delivery systems.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/326431

A vibrational in vitro approach to evaluate the potential of monoolein nanoparticles as isofuranodiene carrier in MDA-MB 231 breast cancer cell line: new insights from Infrared and Raman microspectroscopies
SPECTROCHIMICA ACTA. PART A, MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
Autore/i: Notarstefano, Valentina; Pisani, Michela; Bramucci, Massimo; Quassinti, Luana; Maggi, Filippo; Vaccari, Lisa; Parlapiano, Marco; Giorgini, Elisabetta; Astolfi, Paola
Classificazione: 1 Contributo su Rivista
Abstract: Isofuranodiene (IFD) is a sesquiterpene occurring in several plant species, which proved to have multiple anticancer activities. IFD has a lipophilic nature and, hence, a very low water solubility and a poor bioavailability; moreover, it is not stable, undergoing the “Cope rearrangement” to the less active curzerene. The use of appropriate delivery systems can thus be considered as a valid tool to enhance IFD bioavailability, solubility, stability and at the same time also to improve its intracellular uptake and pharmacological activity. Within this frame, monoolein (GMO) nanoparticles loaded with IFD were prepared and their enhanced anticancer activity, compared to pristine IFD, was assessed. In this study, for the first time, an in vitro Fourier Transform Infrared and Raman Microspectroscopy approaches were exploited to evaluate the effects of IFD, alone and loaded in GMO nanoparticles, on MDA-MB 231 breast cancer cell line. The anti-cancer effects of IFD were evidenced by both the spectroscopic techniques and discriminated from the GMO-induced changes in the culture environment; moreover, a synergistic effect of IFD and GMO administration can be envisaged by the experimental results.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/296601

Characterisation of Bee Pollen from the Marche Region (Italy) According to the Botanical and Geographical Origin with Analysis of Antioxidant Activity and Colour, Using a Chemometric Approach
MOLECULES
Autore/i: Castiglioni, S.; Stefano, M.; Astolfi, P.; Pisani, M.; Carloni, P.
Classificazione: 1 Contributo su Rivista
Abstract: Attempts have often been made to isolate and characterise monofloral pollens to correlate nutritional with botanical properties. Nevertheless, pollen harvested in a particular area that can have a high biodiversity could have healthier properties. In addition, the analysis of the pollen's botanical composition can be important for characterising the typical flora of a specific geographical area. On this basis, various pollens collected in different locations of the Marche region (Italy) and in different harvesting periods were analyzed for botanical composition and antioxidant (total phenolic content, ABTS, DPPH and ORAC tests), granulometry and colour (CIE L*a*b*) properties to evaluate the biodiversity of pollen sources within a particular geographical area and to correlate this to the nutraceutical characteristics. Antioxidant activity results showed values generally higher than those of monofloral pollens harvested in the same areas but manually separated according to colour, shape and size. This suggests that even the floral species present in low percentages may have an influence on the nutraceutical properties of these products. The multivariate statistical elaboration of the obtained results permitted the separation of samples containing a prevalent botanical species and the grouping of all the samples into separate clusters corresponding to different areas of Marche.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/313874

Nanoscale Structure of Langmuir–Blodgett Film of Bent-Core Molecules
NANOMATERIALS
Autore/i: Adamo, F. C.; Ciuchi, F.; De Santo, M. P.; Astolfi, P.; Warner, I.; Scharrer, E.; Pisani, M.; Vita, F.; Francescangeli, O.
Classificazione: 1 Contributo su Rivista
Abstract: Bent-core mesogens (BCMs) are a class of thermotropic liquid crystals featuring several unconventional properties. However, the interpretation and technological exploitation of their unique behavior have been hampered by the difficulty of controlling their anchoring at surfaces. To tackle this issue, we report the nanoscale structural characterization of BCM films prepared using the Langmuir–Blodgett technique. Even though BCMs are quite different from typical amphiphilic molecules, we demonstrate that stable molecular films form over water, which can then be transferred onto silicon substrates. The combination of Brewster angle microscopy, atomic force microscopy, and X-ray reflectivity measurements shows that the molecules, once transferred onto a solid substrate, form a bilayer structure with a bottom layer of flat molecules and an upper layer of upright molecules. These results suggest that Langmuir–Blodgett films of BCMs can provide a useful means to control the alignment of this class of liquid crystals.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/305903

Cubic and Hexagonal Mesophases for Protein Encapsulation: Structural Effects of Insulin Confinement
LANGMUIR
Autore/i: Astolfi, Paola; Giorgini, Elisabetta; Romano Perinelli, Diego; Vita, Francesco; Adamo, FABRIZIO CORRADO; Logrippo, Serena; Parlapiano, Marco; Bonacucina, Giulia; Pucciarelli, Stefania; Francescangeli, Oriano; Vaccari, Lisa; Pisani, Michela
Classificazione: 1 Contributo su Rivista
Abstract: Monoolein-based cubic and hexagonal mesophases were investigated as matrices for insulin loading, at low pH, as a function of temperature and in the presence of increasing amounts of oleic acid, as a structural stabilizer for the hexagonal phase. Synchrotron small angle X-ray diffraction, rheological measurements, and attenuated total reflection-Fourier transform infrared spectroscopy were used to study the effects of insulin loading on the lipid mesophases and of the effect of protein confinement in the 2D- and 3D-lipid matrix water channels on its stability and unfolding behavior. We found that insulin encapsulation has only little effects both on the mesophase structures and on the viscoelastic properties of lipid systems, whereas protein confinement affects the response of the secondary structure of insulin to thermal changes in a different manner according to the specific mesophase: in the cubic structure, the unfolding toward an unordered structure is favored, while the prevalence of parallel β-sheets, and nuclei for fibril formation, is observed in hexagonal structures.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/291792

Antioxidant Activity Level, Bioactive Compounds, Colour and Spectroscopic Analysis (UV-Vis and FT-IR) of Flavoured Drinks Made with Wine and Sour Cherries (Prunus cerasus Var. austera)
FOODS
Autore/i: Pisani, M; Astolfi, P.; Sabbatini, S.; Carloni, P.
Classificazione: 1 Contributo su Rivista
Abstract: In recent years, the increase in consumer interest towards simpler and authentic lifestyles has led to an explosive growth in the production and business of typical agri-food products and, among these, of wines and its derived beverages. With the aim of promoting a typical Italian beverage, the so-called “Vino di visciole” or “Visner”, listed in the national table of traditional agrifood products, the antioxidant and colour properties of fifteen samples from different provinces of the Marche region and obtained with different recipes were analysed. The “in vitro” total antioxidant activity (TAA) determined using ABTS assays, total phenolic content (TPC), total flavonoid content (TFC), total anthocyanins content (TAC), and colour (Somers assay) were measured. In addition, a spectroscopic FT-IR and UV-Vis analysis was carried out to analyse samples with multivariate techniques. The results showed that the production area, the recipe, and the type of cherries used to make the alcoholic beverage do not influence the antioxidant properties and the phytochemical contents of the samples. The multivariate treatment of the spectroscopic features (mainly UV-Vis) rather allowed the differentiation of samples with high antioxidant activity using easy and low-cost instrumental techniques that require little time and can be employed in routine analysis.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/291762

Tailoring non-conventional water resources for sustainable and safe reuse in agriculture
CHEMICAL ENGINEERING TRANSACTIONS
Autore/i: Foglia, A.; Parlapiano, M.; Akyol, G. C. C.; Eusebi, A. L.; Pisani, M.; Astolfi, P.; Fatone, F.
Classificazione: 1 Contributo su Rivista
Abstract: In water scarce areas, like the Mediterranean Region where the lack of irrigation water is already limiting agricultural production, the valorization of non-concventional water resources is utmost important. Accordingly, in this study, we aimed to provide safe and locally sustainable ways of water supply for the Mediterranean agricultural sector by exploiting non-conventional water resources for irrigation purpose. In this context, pilot scale anaerobic reactors treating urban wastewater were operated coupling upflow anaerobıc sludge blanket (UASB) reactor and ultrafiltration anaerobic membrane bioreactor (AnMBR). The resulting permeate is of high quality, accomplishing the EU quality standards for irrigation water reuse, also in terms of E.coli as the main microbial indicator. However, contaminants of emerging concern (CECs) can be a further limitation for safe reuse of the reclaimed water. Hence, molecularly imprinted polymers (MIPs) were further integrated as the final refining step for the selective removal of CECs. Diclofenac was used as the target compound with a removal efficiency up to 50% in the final effluent. In parallel, an intensive pilot system was operated for brine treatment through evaporation, chemical addition and precipitation, and forward osmosis, where up to 77% Mg2+ and 45% Ca2+ recovery rates were achieved. The recovered Mg-salts from the brine treatment were then used as an external source to enhance the struvite precipitation in the N- and P-rich effluent of AnMBR. The preliminary tests showed that only hydroxiapatite salts precipitated without any external P addition.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/294627

Selective removal of contaminants of emerging concern (CECs) from urban water cycle via Molecularly Imprinted Polymers (MIPs): Potential of upscaling and enabling reclaimed water reuse
JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING
Autore/i: Parlapiano, M.; Akyol, C.; Foglia, A.; Pisani, M.; Astolfi, P.; Eusebi, A. L.; Fatone, F.
Classificazione: 1 Contributo su Rivista
Abstract: Contaminants of emerging concern (CECs) are one of the main barriers in the water cycle as they limit the water reuse due to their adverse effects on humans and the ecosystem. Natural and/or engineered ecosystems, such as conventional wastewater treatment processes, are not designed to remove CECs and contribute to the bioaccumulation in organisms considering high volumes of treated water discharges. The adoption of innovative solutions to upgrade urban water cycle facilities has gained relevance for the removal of these substances from final effluents. Molecularly imprinted polymers (MIPs) show promising selective removal toward a wide range of CECs. However, this process is still limited to lab/bench scale and needs to be critically analyzed and assessed for possible scale-up in real environment. Therefore, in this review, an overview of the fate and occurrence of CECs in wastewater is initially reported together with the state-of-the-art adsorption mechanisms to remove these compounds. In the central part of the paper, an evaluation of MIPs synthesis and their status in removing CECs from water matrix are presented. An upscaling pathway of MIPs column from lab- to pilot-scale is given to be applied for enhanced CECs removal and safe water reuse in irrigation/fertigation. Finally, possible integration of MIP columns to real wastewater treatment facilities is discussed and advantages and disadvantages of the potential solutions are addressed to enhance their sustainability.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/294626

Synchrotron characterization of hexagonal and cubic lipidic phases loaded with azolate/phosphane gold(I) compounds: a new approach to the uploading of gold(I)-based drugs
NANOMATERIALS
Autore/i: Astolfi, Paola; Pisani, Michela; Giorgini, Elisabetta; Rossi, Barbara; Damin, Alessandro; Vita, Francesco; Francescangeli, Oriano; Luciani, Lorenzo; Galassi, Rossana
Classificazione: 1 Contributo su Rivista
Abstract: Gold(I) phosphane compounds have recently attracted a renewed interest as potential new protagonists in cancer therapy. A class of phosphane gold(I) complexes containing azolate ligands has been successfully tested against several cancer cell lines and, in particular, against basal-like breast (BLB) cancer, a form characterized by strongly severe diagnosis and short life lapse after classic chemotherapy. Even though the anticancer activity of gold(I) phosphane compounds is thoroughly ascertained, no study has been devoted to the possibility of their delivery in nanovectors. Herein, nonlamellar lyotropic liquid crystalline lipid nanosystems, a promising class of smart materials, have been used to encapsulate gold(I) azolate/phosphane complexes. In particular, ((triphenylphosphine)-gold(I)-(4,5-dichloroimidazolyl-1H-1yl)) (C-I) and ((triphenylphosphine)-gold(I)-(4,5-dicyanoimidazolyl-1H-1yl)) (C-II) have been encapsulated in three different lipid matrices: monoolein (GMO), phytantriol (PHYT) and dioleoyl-phosphatidylethanolamine (DOPE). An integrated experimental approach involving X-ray diffraction and UV resonant Raman (UVRR) spectroscopy, based on synchrotron light and attenuated total reflectance Fourier transform infrared (ATR-FTIR) spectroscopy, has been employed to establish the effects of drug encapsulation on the structure and phase behavior of the host mesophases. The results indicate that gold(I) complexes C-I and C-II are successfully encapsulated in the three lipid matrices as evidenced by the drug-induced phase transitions or by the changes in the mesophase lattice parameters observed in X-ray diffraction experiments and by the spectral changes occurring in UV resonant Raman spectra upon loading the lipid matrices with C-I and C-II.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/284396

Investigation of human pancreatic cancer tissues by Fourier Transform Infrared Hyperspectral Imaging
JOURNAL OF BIOPHOTONICS
Autore/i: Notarstefano, Valentina; Sabbatini, Simona; Conti, Carla; Pisani, Michela; Astolfi, Paola; Pro, Chiara; Rubini, Corrado; Vaccari, Lisa; Giorgini, Elisabetta
Classificazione: 1 Contributo su Rivista
Abstract: Fourier-transform infrared hyperspectral imaging (FTIR-HSI) provides hyperspectral images containing both morphological and chemical information. It is widely applied in the biomedical field to detect tumor lesions, even at the early stage, by identifying specific spectral biomarkers. Pancreatic neoplasms present different prognoses and are not always easily classified by conventional analyses. In this study, tissue samples with diagnosis of pancreatic ductal adenocarcinoma and pancreatic neuroendocrine tumor were analyzed by FTIR-HSI and the spectral data compared with those from healthy and dysplastic samples. Multivariate/univariate approaches were complemented to hyperspectral images, and definite spectral markers of the different lesions identified. The malignant lesions were recognizable both from healthy/dysplastic pancreatic tissues (high values of phospholipids and triglycerides with shorter, more branched and less unsaturated alkyl chains) and between each other (different amounts of total lipids, phosphates and carbohydrates). These findings highlight different metabolic pathways characterizing the different samples, well detectable by FTIR-HSI.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/272050

Nanostructured liquid crystalline particles as delivery vectors for isofuranodiene: Characterization and in-vitro anticancer activity
COLLOIDS AND SURFACES. B, BIOINTERFACES
Autore/i: Pisani, M.; Quassinti, L.; Bramucci, M.; Galassi, R.; Maggi, F.; Rossi, B.; Damin, A.; Carloni, P.; Astolfi, P.
Classificazione: 1 Contributo su Rivista
Abstract: Isofuranodiene is an oxygenated sesquiterpene containing a furan ring isolated from the essential oil of Smyrnium olusatrum L. (Apiaceae) owning notable anticancer activity. Despite its biological potential, the high lipophilicity along with a relatively low stability due to Cope rearrangement giving rise to a less active compound, make the perspective of its therapeutical use unlikely. On this basis, in the present work we evaluated bulk and dispersed non lamellar liquid crystalline phases as effective delivery vectors for isofuranodiene, and capable of preserving its structure and enhancing the biological activity. Small-angle X-ray scattering, dynamic light scattering, and UV resonance Raman spectroscopy were used to characterize the nanosystems in an integrated experimental approach. Encapsulation of isofuranodiene in the lipid matrix resulted in a transition from a cubic Im3m to a reversed hexagonal phase because of the highly lipophilic character of the drug, as obtained in SAXS measurements, and in significant shifts in the components of the Raman spectrum of isofuranodiene. The anticancer activity of isofuranodiene-loaded lipidic nanoparticles was assessed on MDA-MB 231 cell line by MTT assay and was found to be higher than that of pristine isofuranodiene.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/277931

Effects of a cationic surfactant incorporation in phytantriol bulk cubic phases and dispersions loaded with the anticancer drug 5-fluorouracil
JOURNAL OF MOLECULAR LIQUIDS
Autore/i: Astolfi, P.; Giorgini, E.; Adamo, F. C.; Vita, F.; Logrippo, Serena; Francescangeli, O.; Pisani, M.
Classificazione: 1 Contributo su Rivista
Abstract: Ordered lyotropic liquid crystalline phases, which are particularly interesting for several biological and biomedical applications, can show the advantage of having a tunable three-dimensional nanostructure. Addition of ionic surfactants to lipid/water binary systems may influence their phase behavior and their structural properties, such as bilayer thickness, water channel diameter and interfacial curvature, resulting in specific amphiphile self-assembly materials. Understanding the effects of such additives is thus of considerable interest and, in this context, we studied the incorporation of a cationic surfactant (didodecyldimethylammonium bromide) in the phytantriol cubic phase, unloaded and loaded with the anticancer drug 5-fluorouracil. In particular, an anionic form of the drug was also used which could further influence the mesophase structure of the lipid matrix and its entrapment efficiency. The formulations, prepared as bulk and dispersed phases, were characterized by synchrotron small angle X-ray scattering, attenuated total reflection Fourier transform infrared and UV–Vis spectroscopies and dynamic light scattering. Addition of increasing amounts of the cationic surfactant resulted in phase changes following the sequence Pn3m-Im3m-Lα, which was almost unaltered when the drug in its neutral form was encapsulated in the systems. On the contrary, the presence of the anionic 5-fluorouracil stabilized the Pn3m cubic phase at all the cationic surfactant concentrations used.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/267942

Reaction of endogenous Coenzyme Q10 with nitrogen monoxide and its metabolite nitrogen dioxide
REDOX REPORT
Autore/i: Astolfi, P.; Clement, J. -L.; Gigmes, D.; Armeni, T.; Carloni, P.; Greci, L.
Classificazione: 1 Contributo su Rivista
Abstract: Objectives: Coenzyme Q(10), incorporated in DOPC lyposomes or naturally present in liver bovine mitochondria or in human blood plasma, was reacted with nitrogen dioxide (NO2)-N-center dot or with a (NO)-N-center dot/(NO2)-N-center dot mixture. Methods and Results: The reaction course was monitored by Electron Paramagnetic Resonance (EPR) spectroscopy and in all cases the formation of a di-tert-alkyl nitroxide was observed, deriving from the addition of (NO2)-N-center dot to one of the double bonds, most likely the terminal one, of the isoprenic chain. The rate constant for nitroxide formation was also determined by EPR spectroscopy and an initial rate of ca. 7 x 10(-8 )M s(-1) was obtained.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/269538

Morphological, Physicochemical and FTIR Spectroscopic Properties of Bee Pollen Loads from Different Botanical Origin
MOLECULES
Autore/i: Castiglioni, Sara; Astolfi, Paola; Conti, Carla; Monaci, Elga; Stefano, Mariassunta; Carloni, Patricia
Classificazione: 1 Contributo su Rivista
Abstract: Bee pollen loads generally have a homogeneous and monospecific pollen content and assume a typical form and color, due to the typical bee foraging habits, thus having a typical composition related to the botanical origin. The present study aims to characterize bee pollen loads belonging to different botanical species using morphological, spectroscopic and color properties and to find relationships between these variables. IR spectra analysis allowed to have a reliable picture of the components present in the different samples; color and granulometry permits a visual identification of pollen load belonging to different species. Multivariate analysis enabled differentiation among the botanical origin of most of the bee pollen samples, grouping them according to the family and the genus and confirming the possibility to use IR and color measurements for the evaluative analysis and classification of bee pollen samples, to promote the consumption of this bee product as functional food.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/271340

Encapsulation of vitamin B12 into nanoengineered capsules and soft matter nanosystems for targeted delivery
COLLOIDS AND SURFACES. B, BIOINTERFACES
Autore/i: Maiorova, L. A.; Erokhina, S. I.; Pisani, M.; Barucca, G.; Marcaccio, M.; Koifman, O. I.; Salnikov, D. S.; Gromova, O. A.; Astolfi, P.; Ricci, V.; Erokhin, V.
Classificazione: 1 Contributo su Rivista
Abstract: Targeted delivery of vitamins to a desirable area is an active branch in a modern pharmacology. The most important and difficult delivery of vitamin B12 is that to bone marrow and nerve cells. Herein we present a first step towards the development of two types of smart carriers, polymer capsules and lyotropic liquid-crystalline nanosystems, for vitamin B12 targeted delivery and induced release. A vitamin B12 encapsulation technique into nanoengineered polymeric capsules produced by layer-by-layer assembling of polymeric shells on CaCO3 templates has been developed. The effectiveness of the process was demonstrated by optical absorption spectroscopy, transmission electron microscopy (TEM), atomic force microscopy (AFM) and small-angle X-ray diffraction. TEM and AFM analyses performed on capsules after their drying, confirmed the presence of the vitamin B12 inside the capsules in the form of crystalline nanoaggregates, 50–300 nm in diameter. Soft lipid nanovectors consisting of amphiphilic phytantriol molecules, which in water excess spontaneously self-assembly in 3D wellordered inverse bicontinuous cubic bulk phase, were used as alternative carriers for vitamin B12. It was shown that about 30% of the vitamin added in the preparation of the soft lipid system was actually encapsulated in cubosomes and that no structural changes occurred upon loading. The Vitamin stabilizes the lipid system playing the role of its structure-forming element. The biocompatible nature, the stability and the feasibility of these systems make them good candidates as carriers for hydrophilic vitamins.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/269787

Chemometric approach to the analysis of antioxidant properties and colour of typical Italian monofloral honeys.
INTERNATIONAL JOURNAL OF FOOD SCIENCE & TECHNOLOGY
Autore/i: Castiglioni, Sara; Stefano, Mariassunta; Astolfi, Paola; Carloni, Patricia
Classificazione: 1 Contributo su Rivista
Abstract: The antioxidant and colour properties of nine types of monofloral Italian honeys were analysed and correlated with multivariate analysis to find relationships able to characterize the honey floral source. Total phenolic content (TPC) and antioxidant activity of the honey samples increase in the order Citrus ≈ Black Locust < Sunflower ≈ Eucalyptus ≈ Coriander < Fir Honeydew ≈ Chestnut < Honeydew ≈ Strawberry Tree and mainly correlate with colour. A Linear Discriminant Analysis was carried out to choose spectrophotometric λ able to characterize the colour of the different types of honey. Elaboration of data with Cluster and Principal Component Analysis enables differentiation among the floral source of some honey samples, namely Strawberry Tree, Honeydew, Citrus, Black Locust, Chestnut and Fir Honeydew.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/245132

Lyotropic Liquid-Crystalline Nanosystems as Drug Delivery Agents for 5-Fluorouracil: Structure and Cytotoxicity
LANGMUIR
Autore/i: Astolfi, Paola; Giorgini, Elisabetta; Gambini, Valentina; Rossi, Barbara; Vaccari, Lisa; Vita, Francesco; Francescangeli, Oriano; Marchini, Cristina; Pisani, Michela
Classificazione: 1 Contributo su Rivista
Abstract: Lyotropic cubic liquid-crystalline systems have received increasing attention due to their unique microstructural and physicochemical properties as efficient nanocarriers for drug delivery. We report the preparation and characterization of bulk phases and cubosome dispersions of phytantriol loaded with the anticancer drug 5-fluorouracil, in neutral and anionic forms. In both cases, a Pn3m cubic phase was observed. The phytantriol phase behavior can be influenced by the addition of ionic agents, and, to this purpose, a positively charged lipid, such as N-[1-(2,3-dioleoyloxy)propyl]-N,N,N-trimethylammonium chloride salt (DOTAP), was included in the studied formulations. It was found to induce a variation of the spontaneous membrane curvature of the phytantriol lipid bilayer, generating a transition from the Pn3m to the Im3m cubic phase. When 5-fluorouracil, in its anionic form (5-FUs), was encapsulated in these latter systems, a further transition to the HII hexagonal phase was observed as a consequence of the formation of a complex phytantriol/DOTAP/5-FUs. The physicochemical characterization was performed with various complementary techniques including synchrotron small-angle X-ray scattering, dynamic light scattering, and attenuated total reflection Fourier transform infrared and UV resonance Raman spectroscopies. Encapsulation of 5-fluorouracil in the corresponding nanodispersions was evaluated, and their in vitro cytotoxicity was assessed in MDA-MB-231 cell line. Phytantriol cubosomes containing 5-fluorouracil showed a higher toxicity compared with the bare drug solution, and hence they represent potential nanocarriers in the delivery of 5-fluorouracil for cancer therapy.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/251565

Strawberry consumption improves aging-associated impairments, mitochondrial biogenesis and functionality through the AMP-activated protein kinase signaling cascade
FOOD CHEMISTRY
Autore/i: Giampieri, Francesca; Alvarez-Suarez, Josè M.; Cordero, Mario D.; Gasparrini, Massimiliano; Forbes-Hernandez, Tamara Y.; Afrin, Sadia; Santos-Buelga, Celestino; González-Paramás, Ana M.; Astolfi, Paola; Rubini, Corrado; Zizzi, Antonio; Tulipani, Sara; Quiles, Josè L.; Mezzetti, Bruno; Battino, Maurizio
Classificazione: 1 Contributo su Rivista
Abstract: Dietary polyphenols have been recently proposed as activators of the AMP-activated protein kinase (AMPK) signaling pathway and this fact might explain the relationship between the consumption of polyphenol-rich foods and the slowdown of the progression of aging. In the present work, the effects of strawberry consumption were evaluated on biomarkers of oxidative damage and on aging-associated reductions in mitochondrial function and biogenesis for 8 weeks in old rats. Strawberry supplementation increased antioxidant enzyme activities, mitochondrial biomass and functionality, and decreased intracellular ROS levels and biomarkers of protein, lipid and DNA damage (P < 0.05). Furthermore, a significant (P < 0.05) increase in the expression of the AMPK cascade genes, involved in mitochondrial biogenesis and antioxidant defences, was also detected after strawberry intake. These in vivo results were then verified in vitro on HepG2 cells, confirming the involvement of AMPK in the beneficial effects exerted by strawberry against aging progression.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/255284

Strawberry consumption alleviates doxorubicin-induced toxicity by suppressing oxidative stress
FOOD AND CHEMICAL TOXICOLOGY
Autore/i: Giampieri, Francesca; ALVAREZ SUAREZ, JOSE MIGUEL; Gasparrini, Massimiliano; FORBES HERNANDEZ, TAMARA YULIETT; Afrin, Sadia; Bompadre, Stefano; Rubini, Corrado; Zizzi, Antonio; Astolfi, Paola; Santos Buelga, Celestino; González Paramás, Ana M.; Quiles, Josè L.; Mezzetti, Bruno; Battino, Maurizio
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/241687

Liponitroxides: EPR study and their efficacy as antioxidants in lipid membranes
RSC ADVANCES
Autore/i: Mobbili, Giovanna; Crucianelli, Emanuela; Barbon, Antonio; Marcaccio, Massimo; Pisani, Michela; Dalzini, Annalisa; Ussano, Eleonora; Bortolus, Marco; Stipa, Pierluigi; Astolfi, Paola
Classificazione: 1 Contributo su Rivista
Abstract: A series of lipid-functionalized nitroxides having a pyrroline nitroxide moiety linked either to a glycerol or to a steroid unit has been synthesized, and their inclusion inside phospholipid bilayers has been investigated by Electron Paramagnetic Resonance (EPR) spectroscopy. The antioxidant behavior of these nitroxides has been studied in azo-initiator induced lipid peroxidation by means of the Thiobarbituric Acid Reactive Species (TBARS) assay; a correlation with their penetration depth within the bilayer has been found. The possible mechanisms involved in the antioxidant action have been considered, discussed and alternative pathways have been suggested for the synthesized liponitroxides due to their different localization. The steroid derivative is limited to scavenging radicals that are generated in the aqueous phase, while the glycerolipids can also act as chain breaking antioxidants.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/230125

The reactivity of manganese dioxide towards different substrates in organic solvents
NEW JOURNAL OF CHEMISTRY
Autore/i: Alberti, Angelo; Astolfi, Paola; Carloni, Patricia; Greci, Lucedio; Rizzoli, Corrado; Stipa, Pierluigi
Classificazione: 1 Contributo su Rivista
Abstract: The reactivity of manganese dioxide in organic solvents was studied by reacting commercial manganese dioxide MnO2 with different substrates. The reaction of MnO2 with arylimino, indolinonic and quinolinic aromatic nitroxides gave quinoneimine N-oxides via an electron transfer process followed by oxygen transfer. A reduction potential of 0.9 V vs. NHE was roughly estimated for manganese dioxide in aprotic organic solvents by considering the oxidation potentials of those nitroxides able to react with MnO2. By the reaction with para-anisidine the amine radical cation was initially formed through a simple electron transfer process. In the case of substituted phenols, the isolated products were derived instead from the phenoxy radicals generated via hydrogen transfer or an equivalent process. Finally, the quantitative conversion of triphenylphosphine to triphenylphosphine oxide was explained by oxygen atom transfer in a cyclic intermediate derived from an initial nucleophilic attack of phosphorus on MnO2.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/228387

Influence of steeping conditions (time, temperature, and particle size) on antioxidant properties and sensory attributes of some white and green teas
INTERNATIONAL JOURNAL OF FOOD SCIENCES AND NUTRITION
Autore/i: Castiglioni, Sara; Damiani, Elisabetta; Astolfi, Paola; Carloni, Patricia
Classificazione: 1 Contributo su Rivista
Abstract: The influence of commonly used steeping times and temperatures, as well as leaf size on the antioxidant activity and sensory attributes of tea were studied. Five unblended white and green tea samples from China and Malawi, infused in hot (70 C and 90 C; 7 min) or cold water (room temperature: 15, 30, 60, or 120 min) either as whole leaves or as milled, were analyzed. Total phenolic and flavonoid contents as well as antioxidant power (ABTS assay) were measured. The results show that the maximum extraction efficiency occurs with cold water for 120 min and with hot water at 90 C and that only in the case of teas from whole, large leaves, the extraction was greater in cold than in hot infusions. Moreover, tea infusions prepared from milled leaves have the greatest antioxidant activity. In the sensory evaluation of some of the tea infusions, white teas were perceived more fragrant than green ones and were judged as the most favorite by the majority of the judges, especially for the brew prepared in cold water from whole leaves; all infusions obtained from the milled leaves in fact have a more bitter and astringent taste.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/226055

Prevention of UVA-induced oxidative damage in human dermal fibroblasts by new UV filters, assessed using a novel in vitro experimental system.
PLOS ONE
Autore/i: F., Brugè; Tiano, Luca; Astolfi, Paola; Emanuelli, Monica; Damiani, Elisabetta
Classificazione: 1 Contributo su Rivista
Abstract: BACKGROUND: UVA rays present in sunlight are able to reach the dermal skin layer generating reactive oxygen species (ROS) responsible for oxidative damage, alterations in gene expression, DNA damage, leading to cell inflammation, photo-ageing/-carcinogenesis. Sunscreens contain UV filters as active ingredients that absorb/reflect/dissipate UV radiation: their efficiency depends on their spectral profile and photostability which should then be reflected in biological protection of underlying skin. METHODS: A set of new UV filters was synthesized, and the most photostable one was compared to BMDBM, a widely used UVA filter. Cultured human dermal fibroblasts were exposed to UVA radiation which was filtered by a base cream containing or not UV filters placed above cell culture wells. The endpoints measured were: cell viability (MTT assay), ROS generation (DCFH-DA assay), mitochondrial function (JC-1 assay), DNA integrity (Comet assay) and gene expression (MMP-1, COL1A1) by RT-qPCR. RESULTS: The new UV filter resulted more efficient than BMDBM in preserving cell viability, mitochondrial functionality and oxidative DNA damage, despite similar inhibition levels of intracellular ROS. Moreover, expression of genes involved in dermal photoageing were positively affected by the filtering action of the tested molecules. CONCLUSIONS: The experimental model proposed was able to validate the efficacy of the new UV filter, taking into account important cellular events related to UV-induced intracellular oxidative stress, often underestimated in the assessments of these compounds. GENERAL SIGNIFICANCE: The model may be used to compare the actual biological protection of commercial sunscreens and suncare products aside from their SPF and UVA-PF values.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/158507

Strawberry extract protects against oxidative stress and improves mitochondrial functionality in human dermal fibroblasts exposed to hydrogen peroxide.
5th Berry Health Benefits Symposium - Abstracts of Posters
Autore/i: Giampieri, Francesca; ALVAREZ SUAREZ, JOSE MIGUEL; Sara, Tulipani; Ana M., Gonzàles Paramàs; Celestino Santos, Buelga; Astolfi, Paola; Bompadre, Stefano; José L., Quiles; Mezzetti, Bruno; Battino, Maurizio
Classificazione: 4 Contributo in Atti di Convegno (Proceeding)
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/206356

Indolinic nitroxides: evaluation of their potential as universal control agents for nitroxide mediated polymerization
POLYMER CHEMISTRY
Autore/i: Astolfi, Paola; Lucedio, Greci; Stipa, Pierluigi; Corrado, Rizzoli; Cedric, Ysacco; Marion, Rollet; Laurent, Autissier; Antoine, Tardy; Yohann, Guillaneuf; Didier, Gigmes
Classificazione: 1 Contributo su Rivista
Abstract: Indolinic nitroxides derived from 2,2-diphenyl-3-phenylimino-2,3-dihydroindol-1-yloxyl (DPAIO) nitroxide were designed to improve the bulk polymerization of methacrylate derivatives. The corresponding alkoxyamines were prepared by reacting alkyl halide and nitroxide in the presence of tin hydride and PbO2. The replacement of one phenyl ring at the C-2 position on the nitroxide by either a neopentyl or an isopropyl group led to compounds with faster decomposition kinetics and better selectivity for the C–ON bond homolysis. The alkoxyamines derived from these two nitroxides were used to control the polymerization of methyl methacrylate and were also tested for the polymerization of styrene as a model for mono-substituted monomers. The shift of the molar mass distribution during the NMP of styrene showed for the first time that pure homopolymerization of styrene and methacrylate derivatives could be controlled by the same nitroxide, thus opening the way to a universal nitroxide for NMP
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/99862

Benzoxazinic nitrones and nitroxides as possible antioxidants in biological systems
RSC ADVANCES
Autore/i: Astolfi, Paola; Carloni, Patricia; Marini, MARIA GIOVANNA; Mobbili, Giovanna; Pisani, Michela; Stipa, Pierluigi
Classificazione: 1 Contributo su Rivista
Abstract: Benzoxazinic nitrones and nitroxides are good antioxidants in model biological systems (micelles, lipoproteins, proteins, liposomes, sugars). With nitrones, this activity may be attributed to their radical trapping ability, whereas with nitroxides to their radical scavenging properties. In both cases, H-abstraction from the C-2 position of the benzoxazine moiety cannot be ruled out and could, at least in part, account for their antioxidant behaviour. Appropriate DFT calculations have been carried out to better understand the mechanisms of action of the studied compounds.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/136265

Reactions of Nitric Oxide and Nitrogen Dioxide with Coenzymes Q: Involvement of the Isoprenic Chain
ORGANIC & BIOMOLECULAR CHEMISTRY
Autore/i: Astolfi, Paola; Laurence, Charles; Didier, Gigmes; Lucedio, Greci; Corrado, Rizzoli; Federico, Sorana; Stipa, Pierluigi
Classificazione: 1 Contributo su Rivista
Abstract: The formation of a di-tert-alkyl nitroxide has been observed by Electron Spin Resonance during the exposure of coenzyme CoQ10, in both the oxidized and reduced forms, to nitrogen dioxide (˙NO2) or to nitric oxide (˙NO) in the presence of oxygen. The same kind of nitroxide has been observed also with CoQ1, CoQ3 or with 1-phenyl-3-methyl-2-butene, chosen as model compounds. In all cases, the formation of the nitroxide may be justified only by admitting the involvement of the isoprenic chain of the coenzymes and in particular the addition of ˙NO2 to the double bond. A mechanism which accounts for the formation of the nitroxide as well as the other compounds observed in the reactions is proposed and confirmed by a spectroscopic investigation (FT-IR, 1H NMR, X-ray analysis) and by ESI-MS.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/86373

New Nitroxide functionalized Lipids with Antioxidant Activity
VIII Convegno Nazionale AICIng - Atti del Convegno
Autore/i: C., Petrocchi; Astolfi, Paola; Carloni, Patricia; Mobbili, Giovanna; Pisani, Michela; Stipa, Pierluigi
Editore: Edi-Ermes
Classificazione: 4 Contributo in Atti di Convegno (Proceeding)
Abstract: A new class of different lipids bearing a nitroxide radical moiety was synthesized and used in the preparation of multilamellar phosphatidylcholine liposomes. Analysis of the corresponding EPR spectra allowed to get information about the distribution of these free radicals within the liposome structure, and the resulting antioxidant activity was measured by means of the TBARS assay. A correlation between the functionalised lipids antioxidant activity, their dipolar moment (computed by DFT) and their EPR features could be envisaged.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/80089

Radical-scavenging Activity, Protective Effect Against Lipid Peroxidation and Mineral Contents of Monofloral Cuban Honeys
PLANT FOODS FOR HUMAN NUTRITION
Autore/i: José M., Alvarez Suarez; Giampieri, Francesca; Damiani, Elisabetta; Astolfi, Paola; Fattorini, Daniele; Regoli, Francesco; José L., Quiles; Battino, Maurizio
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/73468

Synthesis and Antioxidant Activity of Nitroxide Functionalized Lipids
XI Convegno Nazionale GIRSE & 1st Joint Meeting ARPE-GERPE-GIRSE
Autore/i: Astolfi, Paola; Carloni, Patricia; Mobbili, Giovanna; Petrocchi, C.; Pisani, Michela; Stipa, Pierluigi
Editore: Anna Longo e Maurizio Marrale
Classificazione: 4 Contributo in Atti di Convegno (Proceeding)
Abstract: Liposomes are biodegradable and nonimmunogenic vesicular structures made of amphipatic phospholipids which can encapsulate both hydrophilic and hydrophobic materials; because of their versatility they are used as carriers in drugs and gene delivery systems. Polyunsaturated fatty acids present in the bilayer are susceptible to oxidative damage which can alter some important properties like fluidity and permeability and can cause system delivery destruction. The mechanism of lipids oxidative degradation may involve free radical reactions that lead to changes in the unsaturated systems present and eventually to the degradation of the lipid chains. In order to maintain their physical, chemical and functional properties, liposomes need to be protected by antioxidant compounds that can delay or prevent oxidation upon reaction with radical species. Nitroxide radicals (paramagnetic compounds with the unpaired electron mainly localized on the N-O group) are known to protect biological system against oxidative processes in different media and under different stress conditions. [1] As part of our ongoing research in the field of nitrones [2] and nitroxides [3] as potential antioxidants we synthesized neutral non zwitterionic lipids labeled by nitroxide able to react with radical species. Multilamellar liposomes were prepared by mixing the new lipids with Phosphatidylcholine and their EPR features and antioxidant activity was studied.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/80538

Phenolics from monofloral honeys protect human erythrocyte membranes against oxidative damage
FOOD AND CHEMICAL TOXICOLOGY
Autore/i: José M., Alvarez Suarez; Giampieri, Francesca; Ana M., González Paramás; Damiani, Elisabetta; Astolfi, Paola; Gregorio Martinez, Sanchez; Bompadre, Stefano; José L., Quiles; Celestino Santos, Buelga; Battino, Maurizio
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/73271

On the assessment of photostability of sunscreens exposed to UVA irradiation: from glass plates to pig/human skin, which is best?
INTERNATIONAL JOURNAL OF PHARMACEUTICS
Autore/i: Crovara Pescia, A; Astolfi, Paola; Puglia, C; Bonina, F; Perrotta, R; Herzog, B; Damiani, Elisabetta
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/65667

Benzoxazinic Nitrones and Nitroxides as Antioxidants in Biological Systems
6th International Conference on Nitroxide Radicals “SPIN 2011”
Autore/i: Astolfi, Paola; Carloni, Patricia; M. G., Marini; Mobbili, Giovanna; Pisani, Michela; Stipa, Pierluigi
Luogo di pubblicazione: Marseille
Classificazione: 4 Contributo in Atti di Convegno (Proceeding)
Abstract: Comunicazione Scientifica al 6th International Conference on Nitroxide Radicals "SPIN 2011" - Marseille (France)
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/62375

Synthesis and Antioxidant Properties of Benzoxanic Nitrones and Nitroxides
XXIV Congresso Nazionale della Società Chimica Italiana
Autore/i: Mobbili, Giovanna; Astolfi, Paola; Carloni, Patricia; M. G., Marini; Pisani, Michela; Stipa, Pierluigi
Classificazione: 4 Contributo in Atti di Convegno (Proceeding)
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/83391

Antioxidant effect of nitroxide functionalized lipids in liposomes peroxidation
XXIV Congresso Nazionale della Società Chimica Italiana
Autore/i: Pisani, Michela; Astolfi, Paola; Carloni, Patricia; Mobbili, Giovanna; C., Petrocchi; Stipa, Pierluigi
Classificazione: 4 Contributo in Atti di Convegno (Proceeding)
Abstract: Cominicazione Scientifica al XXIV Congresso Nazionale della Società Chimica Italiana, Lecce (Italy)
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/83390

Synthesis and Thermal Stability of Benzoxazine Nitroxides
JOURNAL OF ORGANIC CHEMISTRY
Autore/i: Astolfi, Paola; Stipa, Pierluigi
Classificazione: 1 Contributo su Rivista
Abstract: A new class of stable nitroxides (aminoxyls) having a 1,4-benzoxazine structure were synthesized and the corresponding thermal stability tested. All derivatives resulted stable in the whole range of temperature employed, except those having a benzyl or a t-butyl group at the -position with respect to the aminoxyl function, which underwent radical fragmentation. Such a behavior allowed a kinetic study, carried out by means of EPR spectroscopy, to determine the corresponding rate constants and activation parameters (Ea). Appropriate DFT calculations were performed for all nitroxides including also the thermally stable ones, in order to study the geometries of the fragmentation Transition States as well as to compute the corresponding Bond Dissociation Enthalpies (BDH), useful for further modeling purposes. The data obtained were interpreted on the basis of the relative stability of the leaving radical, according to the corresponding Ea and BDH, whereas in the case of t-butyl derivatives steric hindrance should play a determinant role, as evidenced by a comparison of the geometric, kinetic and thermodynamic parameters upon the whole series.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/62749

RADICAL INTERMEDIATES IN THE PHOTOREARRANGEMENT OF 3-HYDROXYINDOLIC NITRONES
TETRAHEDRON
Autore/i: Alberti, A.; Astolfi, Paola; Carloni, Patricia; Döpp, D.; Greci, L.; Rizzoli, C.; Stipa, Pierluigi
Classificazione: 1 Contributo su Rivista
Abstract: A number of 3-hydroxy substituted indolic nitrones were found to quantitatively photorearrange to (2-benzoylamino)phenyl ketones upon UV-A irradiation. EPR-Spin Trapping experiments suggest that the process, which is believed to proceed via the formation of an oxaziridine, follows a homolytic pathway. Although DFT calculations do not allow to totally exclude alternative routes involving singlet intermediates, they do provide support to the proposed homolytic mechanism, which would be driven by a 1,5-hydrogen transfer subsequent to the oxaziridine ring opening. When this hydrogen transfer was made impossible by methylation of the 3-OH group, a benzoxazine was isolated as the sole reaction product.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/57487

Nitroxides and a nitroxide-based UV filter have the potential to photoprotect UVA-irradiated human skin fibroblasts against oxidative damage
JOURNAL OF DERMATOLOGICAL SCIENCE
Autore/i: E., Venditti; F., Brugè; Astolfi, Paola; I., Kochevar; Damiani, Elisabetta
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/54921

Strawberry Polyphenols Attenuate Ethanol-Induced Gastric Lesions in Rats by Activation of Antioxidant Enzymes and Attenuation of MDA Increase.
PLOS ONE
Autore/i: Alvarez Suarez, Jm; Dekanski, D; Ristić, S; Radonjić, Nv; Petronijević, Nd; Giampieri, Francesca; Astolfi, Paola; González Paramás, Am; Santos Buelga, C; Tulipani, S; Quiles, Jl; Mezzetti, Bruno; Battino, Maurizio
Classificazione: 1 Contributo su Rivista
Abstract: Gli estratti polifenolici di differenti varietà di fragole sono in grado di proteggere la mucosa gastrica dall'insulto provocato dall'etanolo limitando gli eventi ulcerosi da questo indotti. L'attività protettiva si esplica tramite l'attivazione di specifici enzimi antiossidanti.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/63394

Cyclic Voltammetry, spectroelectrochemistry and electron spin resonance as combined tools to study thymoquinone in aprotic medium
ELECTROCHIMICA ACTA
Autore/i: Petrucci, R.; Marrosu, G.; Astolfi, Paola; Lupidi, G.; Greci, Lucedio
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/66105

Synthesis and Antioxidant Properties of Benzoxanic Nitrones and Nitroxides
Autore/i: Mobbili, Giovanna; Astolfi, Paola; Carloni, Patricia; M. G., Marini; Pisani, Michela; Stipa, Pierluigi
Luogo di pubblicazione: Lecce
Classificazione: 5 Altro
Abstract: Cominicazione Scientifica al XXIV Congresso Nazionale della Società Chimica Italiana, Lecce (Italy)
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/62372

Could Co-enzyme Q10 be considered a detoxifying agent towards nitrogen dioxides?
Second International Conference on Environmental Stressors in Biology and Medicine
Autore/i: L., Greci; L., Charles; Astolfi, Paola; Damiani, Elisabetta; D., Gigmes; G. P., Littarru
Classificazione: 4 Contributo in Atti di Convegno (Proceeding)
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/83394

Indolinonic Nitroxides as a Backdoor in the evaluation of the Redox Potential of Manganese Dioxide
Autore/i: Greci, Lucedio; Astolfi, Paola; Stipa, Pierluigi; A., Alberti
Classificazione: 5 Altro
Abstract: Comunicazione Scientifica al 6th International Conference on Nitroxide Radicals "SPIN 2008" - Marseille (France)
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/62414

Indolinic Nitroxides forr Nitroxide Mediated Polymerization
Autore/i: L., Autissier; Greci, Lucedio; Astolfi, Paola; Stipa, Pierluigi; Y., Guillaneuf; D., Bertin; D., Gigmes
Classificazione: 5 Altro
Abstract: Comunicazione Scientifica al 6th International Conference on Nitroxide Radicals "SPIN 2008" - Marseille (France)
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/62374

Is the isoprenic chain of Co-enzyme Q10 and its analogues involved in their reactivity with nitric oxide and nitrogen dioxide
6th International Conference of the CoQ10 Association
Autore/i: L., Greci; L., Charles; Astolfi, Paola; Damiani, Elisabetta; D., Gigmes; G. P. L. i. t. t. a. r. u., C.
Classificazione: 4 Contributo in Atti di Convegno (Proceeding)
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/83399

Antioxidant and antimicrobial capacity of several monofloral Cuban honeys and their correlation with color, polyphenol content and other chemical compounds
FOOD AND CHEMICAL TOXICOLOGY
Autore/i: ALVAREZ SUAREZ, Jm; Tulipani, S; Díaz, D; Estevez, Y; Romandini, S; Giampieri, F; Damiani, Elisabetta; Astolfi, Paola; Bompadre, Stefano; Battino, Maurizio
Editore: Elsevier Science Limited:Oxford Fulfillment Center, PO Box 800, Kidlington Oxford OX5 1DX United Kingdom:011 44 1865 843000, 011 44 1865 843699, EMAIL: asianfo@elsevier.com, tcb@elsevier.co.UK, INTERNET: http://www.elsevier.com, http://www.elsevier.com/locate/shpsa/, Fax: 011 44 1865 843010
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/34645

The Reaction of spiro[indoline-naphtopyrans] with nitrogen oxides. Not a case of biradical double trapping
MAGNETIC RESONANCE IN CHEMISTRY
Autore/i: Alberti, A.; Astolfi, Paola; Campredon, M.; Greci, Lucedio; Guerra, M.; Macciantelli, D.; Plescia, E.
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/57599

Assessment of the photo-degradation of UV-filters and radical-induced peroxidation in cosmetic sunscreen formulations.
FREE RADICAL RESEARCH
Autore/i: Damiani, Elisabetta; Astolfi, Paola; J., Giesinger; T., Ehlis; B., Herzog; L., Greci; W., Baschong
Editore: Taylor & Francis Limited:Rankine Road, Basingstoke RG24 8PR United Kingdom:011 44 1256 813035, EMAIL: madeline.sims@tandf.co.uk, info@tandf.co.uk, INTERNET: http://www.tandf.co.uk, Fax: 011 44 1256 330245
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/38129

3-Benzyl-3-hydroxy-2-phenyl-3H-indole 1-oxide
ACTA CRYSTALLOGRAPHICA SECTION E-STRUCTURE REPORTS ONLINE
Autore/i: Rizzoli, Corrado; Astolfi, Paola; Marku, Elda; Greci, Lucedio
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/239178

EPR detection of unexpected imminoxy radicals reacting reversible dyes and nitric oxide
43rd Annual International Meeting of the Electron Spin Resonance Spectroscopy Group of the Royal Society of Chemistry
Autore/i: A., Alberti; Astolfi, Paola; M., Campredon; L., Greci; M., Guerra; D., Macciantelli; E., Plescia
Classificazione: 4 Contributo in Atti di Convegno (Proceeding)
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/83400

Thermal Stability of Alcoxyamines to be used in Radical Polymerizations
Autore/i: Astolfi, Paola; L., Autissier; D., Gigmes; Greci, Lucedio; Stipa, Pierluigi
Classificazione: 5 Altro
Abstract: Comunicazione Scientifica al XXXIII National Congress of the Organic Chemistry Division of the Italian Chemical Society, S. Benedetto del Tronto (Italy), 2010
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/58102

Alcoxyamines from Indolinonic Nitroxides as Controllers for Radical Polymerizations
Autore/i: Astolfi, Paola; L., Autissier; D., Gigmes; Greci, Lucedio; Stipa, Pierluigi
Classificazione: 5 Altro
Abstract: Comunicazione Scientifica al Congresso AICING '10, Bressanone (Italy), 2010
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/58100

Attività antiossidante di infusi di tè: effetto della lavorazione (tè bianco, verde e nero) e del metodo di infusione
PROGRESS IN NUTRITION
Autore/i: Carloni, Patricia; Astolfi, Paola; Tiano, Luca; Bacchetti, Tiziana; Damiani, Elisabetta
Classificazione: 1 Contributo su Rivista
Abstract: Il tè, dopo l’acqua è la bevanda più bevuta al mondo e riceve continuamente attenzione per i numerosi effetti benefici che sembra siano associati con un suo consumo regolare. Tutte le varietà di tè vengono prodotte dalle foglie della Camellia sinensis (L.) della famiglia delle Theaceae. La composizione e la qualità del tè dipendono sia dal tipo di cultivar e dalle condizioni ambientali (terreno, clima, altitudine) che dalle diverse tecniche di raccolta e di trasformazione che portano alla produzione di tè neri (fermentati), oolong (semi-fermentati), verdi e bianchi (non fermentati). In questo studio sono stati valutati, il contenuto totale di polifenoli, l’attività antiossidante e chelante di cinque tè provenienti da una stessa piantagione del Malawi, ma processati in modo diverso per ottenere due tè neri (orthodox e CTC), due tè verdi (decaffeinato e non decaffeinato) e un tè bianco per cercare di capire l’effetto della lavorazione e del periodo di raccolta sull’attività antiossidante di una singola cultivar (cresciuta e raccolta nelle stesse condizioni). Inoltre in considerazione del fatto che i metodi per preparare il tè variano da paese a paese, si è cercato di determinare se l’attività antiossidante di tè diversi, in particolare il tè bianco, può dipendere anche dalla temperatura dell’acqua in cui le foglie del tè vengono infuse.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/74994

Stabilità Termica di Alcossiammine da impiegare nelle Polimerizzazioni Radicaliche
Autore/i: Astolfi, Paola; L., Autissier; D., Gigmes; Greci, Lucedio; Stipa, Pierluigi
Classificazione: 5 Altro
Abstract: Comunicazione Scientifica al 9th Congress of the Italian ESR Group (GIRSE), Rimini (Italy), 2010
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/58101

STUDIO DELL’ATTIVITÀ ANTIOSSIDANTE DI INFUSI DI TÈ BIANCO, ESTRATTI A CALDO E A TEMPERATURA AMBIENTE
Atti del XIX Convegno Interregionale TUMA 2010 – Ancona, 30 settembre-1 ottobre 2010
Autore/i: Carloni, Patricia; Rizzo, A.; Astolfi, Paola; Damiani, Elisabetta
Classificazione: 4 Contributo in Atti di Convegno (Proceeding)
Abstract: Il tè, dopo l’acqua è la bevanda più bevuta al mondo e negli ultimi anno si stanno mettendo in evidenza le sue proprietà antiossidanti e benefiche associate alla prevenzione dello stress ossidativo. Tutte le varietà di tè vengono prodotte dalle foglie della Camellia sinensis della famiglia delle Theaceae. La composizione e la qualità del tè dipendono sia dal tipo di cultivar e dalle condizioni ambientali (terreno, clima, altitudine) che dalle diverse tecniche di raccolta e di lavorazione che portano alla produzione di tè neri, oolong, verdi e bianchi. In uno studio effettuato recentemente1 abbiamo evidenziato come l’infusione di foglie di tè provenienti da diverse regioni e lavorate diversamente, effettuata a temperatura ambiente per due ore, produce soluzioni con proprietà antiossidanti del tutto simili a quella effettuata con acqua calda a 95°C per 7 minuti. Inoltre si è visto che nel caso del tè bianco da noi utilizzato per tale analisi, l’infusione a temperatura ambiente dava risultati anche migliori. In questo studio abbiamo quindi pensato di verificare se tale risultato fosse una esclusiva del tè da noi studiato o fosse una prerogativa dei tè bianchi in generale, andando ad analizzare tè bianchi provenienti da diverse zone.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/74997

Synthesis, structural and spectroscopic characterization and biomimetic properties of new copper, manganese, zinc complexes: identification of possible superoxide-dismutase mimics bearing hydroxyl radical generating/scavenging abilities.
JOURNAL OF INORGANIC BIOCHEMISTRY
Autore/i: G., Lupidi; F., Marchetti; N., Masciocchi; D., Reger; S., Tabassum; Astolfi, Paola; Damiani, Elisabetta; C., Pettinari
Classificazione: 1 Contributo su Rivista
Abstract: J Inorg Biochem. 2010 Aug;104(8):820-30. Epub 2010 Apr 7. Synthesis, structural and spectroscopic characterization and biomimetic properties of new copper, manganese, zinc complexes: identification of possible superoxide-dismutase mimics bearing hydroxyl radical generating/scavenging abilities. Lupidi G, Marchetti F, Masciocchi N, Reger DL, Tabassum S, Astolfi P, Damiani E, Pettinari C. Source Dipartimento di Biologia Molecolare Cellulare e Animale, Università degli Studi di Camerino, Via Madonna delle carceri, I-62032 Camerino, MC, Italy. Abstract A series of Cu(II), Zn(II) and Mn(II) coordination compounds has been synthesized by reaction of the corresponding metal salts and pyrazolyl-based ligands, i.e. the neutral 1-(2-(4-((2,2,2-tri(1H-pyrazol-1-yl)ethoxy)methyl)benzyloxy)-1,1-di(1H-pyrazol-1-yl)ethyl)-1H-pyrazole {p-C(6)H(4)[CH(2)OCH(2)C(pz)(3)](2), (L(1)), and the anionic hydridotris(3-phenyl-5-methylpyrazolyl)borate (L(2))(-), bis(pyrazolyl)acetate (L(3)) and bis(3,5-dimethylpyrazolyl)acetate (L(4))(-): the species [L(1)(CuCl(2))(2)] (1), [L(1)(Cu(OAc)(2))(2)] (2), [L(1)(Zn(OAc)(2))(2)] (3), [(CuCl(L(2))(Hpz(Ph,Me))] (4), [Mn(L(3))(2)].2H(2)O, (5), [ZnCl(L(3))(imH)].MeOH [CuCl(L(4))(imH)].2H(2)O (7) have been obtained (Hpz(Ph,Me)=3-phenyl-5-methylpyrazole, imH=imidazole). Complexes 1 and 4 have been structurally characterized, also using less conventional powder diffraction methods. The superoxide scavenging activity has been characterized by indirect assays including EPR analysis. All complexes exhibit superoxide scavenging activity with IC(50) in the microM range and they protect against the oxidative action of peroxynitrite in different ways. 1, 4 and 7 exert both an anti- and pro-oxidant effect depending on their concentration as evaluated by EPR and fluorescence methods. The pro-oxidative effects are absent in Zn(II) and Mn(II) complexes. (c) 2010 Elsevier Inc. All rights reserved. PMID: 20452028 [PubMed - indexed for MEDLINE]
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/38328

2,2-Diphenylbenzo[c]quinoline-1-oxyl
ACTA CRYSTALLOGRAPHICA SECTION E-STRUCTURE REPORTS ONLINE
Autore/i: Rizzoli, C.; Marku, E.; Greci, Lucedio; Astolfi, Paola
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/57624

Thermal stability of a new class of benzoxazine nitroxides
Autore/i: Astolfi, Paola; Stipa, Pierluigi
Classificazione: 5 Altro
Abstract: Comunicazione Scientifica al 7th Meeting of the European Federation of EPR Groups, Antwerp (Belgium)
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/58240

Stabilità Termica di una nuova classe di Nitrossidi Radicali a struttura Benzossazinica
Autore/i: Astolfi, Paola; Stipa, Pierluigi
Classificazione: 5 Altro
Abstract: Comunicazione Scientifica al Regional Congress of the Italian Chemical Society “TUMA 2009”, Tirrenia (Italy)
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/58239

Antioxidants: how they work
Oxidants in Biology: a question of balance
Autore/i: Damiani, Elisabetta; Astolfi, Paola; Carloni, Patricia; Stipa, Pierluigi; Greci, Lucedio
Editore: Springer Science & Business Media
Classificazione: 2 Contributo in Volume
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/50499

Reactions of Nitrosoarenes with Nitrogen Monoxide (Nitric Oxide) and Nitrogen Dioxide: Formation of Diarylnitroxides.
EUROPEAN JOURNAL OF ORGANIC CHEMISTRY
Autore/i: Astolfi, Paola; Carloni, Patricia; Damiani, Elisabetta; Greci, Lucedio; M., Marini; C., Rizzoli; Stipa, Pierluigi
Classificazione: 1 Contributo su Rivista
Abstract: Nitrosoarenes react with nitrogen monoxide (nitric oxide) at room temperature and in aprotic media to afford the corresponding diarylnitroxides by the intermediate formation of N-nitrosoarylnitroxides. However, these latter spin adducts, in contrast with literature reports, have never been detected by us. N-nitrosophenylnitroxide is obtained only in the oxidation of the ammonium salt of N-nitrosophenylhydroxylamine (cupferron) with trace amounts of lead tetraacetate. However, it evolves with time to diphenylnitroxide, as demonstrated by following the reaction course in the ESR cavity, On a macroscale level, the reaction between nitrosobenzene and nitric oxide leads to the formation of N-nitrosodiphenylamine, 4-nitro-N-nitrosodiphenylamine, 4-nitrodiphenylamine and 4,4'-dinitrodiphenylamine, in addition to diphenylnitroxide. Diarylnitroxides are also obtained when nitrosoarenes react with small amounts of nitrogen dioxide; the mechanism of this reaction is proposed and discussed. The structure of 4-nitro-N-nitrosodiphenylamine was determined by X-ray analysis.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/51506

Sunscreens Cause Coral Bleaching by Promoting Viral Infections
ENVIRONMENTAL HEALTH PERSPECTIVES
Autore/i: Danovaro, Roberto; Bongiorni, Lucia; Corinaldesi, Cinzia; Giovannelli, D; Damiani, Elisabetta; Astolfi, Paola; Greci, Lucedio; Pusceddu, Antonio
Classificazione: 1 Contributo su Rivista
Abstract: Environ Health Perspect. 2008 Apr;116(4):441-7. Sunscreens cause coral bleaching by promoting viral infections. Danovaro R, Bongiorni L, Corinaldesi C, Giovannelli D, Damiani E, Astolfi P, Greci L, Pusceddu A. Source Department of Marine Sciences, Faculty of Science, Polytechnic University of the Marche, Via Brecce Bianche, 60131 Ancona, Italy. r.danovaro@univpm.it Abstract BACKGROUND: Coral bleaching (i.e., the release of coral symbiotic zooxanthellae) has negative impacts on biodiversity and functioning of reef ecosystems and their production of goods and services. This increasing world-wide phenomenon is associated with temperature anomalies, high irradiance, pollution, and bacterial diseases. Recently, it has been demonstrated that personal care products, including sunscreens, have an impact on aquatic organisms similar to that of other contaminants. OBJECTIVES: Our goal was to evaluate the potential impact of sunscreen ingredients on hard corals and their symbiotic algae. METHODS: In situ and laboratory experiments were conducted in several tropical regions (the Atlantic, Indian, and Pacific Oceans, and the Red Sea) by supplementing coral branches with aliquots of sunscreens and common ultraviolet filters contained in sunscreen formula. Zooxanthellae were checked for viral infection by epifluorescence and transmission electron microscopy analyses. RESULTS: Sunscreens cause the rapid and complete bleaching of hard corals, even at extremely low concentrations. The effect of sunscreens is due to organic ultraviolet filters, which are able to induce the lytic viral cycle in symbiotic zooxanthellae with latent infections. CONCLUSIONS: We conclude that sunscreens, by promoting viral infection, potentially play an important role in coral bleaching in areas prone to high levels of recreational use by humans. PMID: 18414624 [PubMed - indexed for MEDLINE] PMCID: PMC2291018
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/40538

Synthesis of Alkoxyamines: A New Method
Autore/i: Astolfi, Paola; D., Bertin; D., Gigmes; Greci, Lucedio; Stipa, Pierluigi
Classificazione: 5 Altro
Abstract: Comunicazione Scientifica al 5th International Conference on Nitroxide Radicals "SPIN 2008" - Ancona (Italy)
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/58242

Sintesi e caratterizzazione di nuovi nitrossidi stabili a struttura benzossazinica
Autore/i: Astolfi, Paola; M., Marini; Stipa, Pierluigi
Classificazione: 5 Altro
Abstract: Comunicazione Scientifica al 8th Congress of the Italian ESR Group (GIRSE), Giovinazzo (Italy)
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/58246

Diphenylnitroxide and Diphenylamino Derivatives from the Reactions of Nitrogen Monoxide and Nitrogen Dioxide with Nitrosobenzene
Book of Abstract
Autore/i: Astolfi, Paola; Carloni, Patricia; Damiani, Elisabetta; L., Greci; M., Marini; Stipa, Pierluigi
Classificazione: 4 Contributo in Atti di Convegno (Proceeding)
Abstract: Recently, we observed that when nitroarenes were reacted with nitrogen monoxide •NO and nitrogen dioxide •NO2 inside the e.s.r. cavity the signals of the corresponding diphenylnitroxides were detected. In order to better understand the reactivity of these two radical species, •NO and •NO2, which can both have deleterious effects (nitrosative and oxidative stress), we tried to obtain further insights on this reaction by carrying it out also on a macroscale level. The isolated compounds, reported in the Scheme, were all justified through the intermediate formation of a diphenylnitroxide. A mechanism for the reaction is proposed in this communication and it is also supported by the data already reported in the literature concerning the reaction between indolinonic nitroxides and nitric oxide.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/57705

Nitroxide-based UV filters: a new strategy against UV-damage?
HOUSEHOLD AND PERSONAL CARE TODAY
Autore/i: Damiani, Elisabetta; Astolfi, Paola; Greci, L.
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/57489

In vitro photostability and photoprotection studies of a novel 'multi-active' UV-absorber.
FREE RADICAL BIOLOGY & MEDICINE
Autore/i: E., Venditti; Spadoni, Tatiana; Tiano, Luca; Astolfi, Paola; Greci, Lucedio; Littarru, GIAN PAOLO; Damiani, Elisabetta
Classificazione: 1 Contributo su Rivista
Abstract: This paper reports on the synthesis and properties of a new UV-absorber (OC-NO) based on the most popular UV filter worldwide, ethylhexyl methoxycinnamate (OMC) in which the methoxy group has been replaced with a pyrrolidine nitroxide bearing antioxidant activity. This sunscreen active has therefore both UV-absorbing and antioxidant properties which could ideally address both the UV-B and UV-A skin photo-damage. For broad-spectrum coverage, the combinations of OC-NO with two commonly used UV-A absorbers (BMDBM and DHHB) were also studied. The results obtained reveal that OC-NO: (a) is as photostable as OMC after UV-A exposure; (b) acts as free radical scavenger as demonstrated by EPR and chemical studies; (c) reduces UV-A and UV-A+BMDBM induced lipid peroxidation in liposomes and cells, measured as reduced TBARS levels and increased C11-BODIPY red fluorescence, respectively; (d) has comparable antioxidant activity to that of vitamin E and BHT commonly used in skin care formulations; (e) is non-cytotoxic to human skin fibroblasts as assessed with the MTT assay when exposed to increasing doses of UV-A; and (f) OC-NO+DHHB is a promising, photostable broad spectrum UV-filter combination that concomitantly reduces UV-induced free radical damage. These results suggest that nitroxide/antioxidant-based UV-absorbers may pave the way for the utilization of 'multi-active' ingredients in sunscreens thereby reducing the number of ingredients in these formulations.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/71863

In vitro photostability and photoprotection studies of a novel 'multi-active' UV-absorber
IV Convegno Nazionale Congiunto di Fotobiologia e Fotochimica
Autore/i: Elisabetta, Venditti; Tatiana, Spadoni; Luca, Tiaon; Astolfi, Paola; Lucedio, Greci; Gian Paolo, Littarru; Elisabetta, Damiani
Classificazione: 4 Contributo in Atti di Convegno (Proceeding)
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/83377

Nitroxide-Mediated Polymerization of Methyl Methacrylate: Is the Challenge Overcome?
29th ASP- Australian Polymer Symposium
Autore/i: Y., Guillaneuf; S. R. A., Marque; Astolfi, Paola; L., Greci; D., Gigmes; P., Tordo; D., Bertin
Classificazione: 4 Contributo in Atti di Convegno (Proceeding)
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/83370

Radical trapping properties of 3-aryl-2H-benzo[1,4]oxazin-4-oxides
JOURNAL OF ORGANIC CHEMISTRY
Autore/i: Astolfi, Paola; M., Marini; Stipa, Pierluigi
Classificazione: 1 Contributo su Rivista
Abstract: A series of 3-aryl-2H-benzo[1,4]oxazin-4-oxides was prepared and their ability to trap free radicals was investigated by EPR spectroscopy. In organic solvents, these compounds were able to efficiently scavenge all carbon- and oxygen-centered radicals tested giving very persistent aminoxyls, except with superoxide anion whose spin adducts were unstable. The main feature of these nitrones as spin traps lies in the possibility to recognize the initial radical trapped. In fact, besides a g-factor and aminoxyl nitrogen EPR coupling constant dependent on the species trapped, the EPR spectra also show different patterns due to hyperfine splittings characteristic of the radical scavenged. This last important feature was investigated by means of Density Functional Theory (DFT) calculations.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/32154

A spectrochemical and chemical study on oxidation of hydroxycinnamic acids in aprotic medium
ELECTROCHIMICA ACTA
Autore/i: Petrucci, R.; Astolfi, Paola; Greci, Lucedio; Firuzi, O.; Saso, L.; Marrosu, G.
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/57622

3-Aryl-2H-benzo[1,4]oxazin-4-oxides: a mew class of spin traps
Autore/i: Astolfi, Paola; M., Marini; Stipa, Pierluigi
Classificazione: 5 Altro
Abstract: Comunicazione Scientifica al GIRSE-ARPE 1st Joint Meeting, Vietri sul Mare (Italy)
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/58251

First Effective Nitroxide-Mediated Polymerization of Methyl Methacrylate
MACROMOLECULES
Autore/i: Guillaneuf, Y.; Gigmes, D.; Marque, S. R. A.; Astolfi, Paola; Greci, Lucedio; Tordo, P.; Bertin, D.
Editore: American Chemical Society:1155 Sixteenth Street Northwest:Washington, DC 20036:(800)227-5558, (800)333-9511, Fax: (202)872-4615
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/57600

Controlled Radical Polymerization of MMA: A Significant Improvement in NMP
EUCHEM Conference on Organic Free Radical
Autore/i: Y., Guillaneuf; S. R. A., Marque; Astolfi, Paola; L., Greci; D., Gigmes; P., Tordo; D., Bertin
Classificazione: 4 Contributo in Atti di Convegno (Proceeding)
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/83365

Reaction of Indoles and Nitrogen Dioxide and Nitrous Acid in Aprotic Solvents
ORGANIC & BIOMOLECULAR CHEMISTRY
Autore/i: Astolfi, Paola; Panagiotaki, M.; Rizzoli, C.; Greci, Lucedio
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/57618

Synthesis and application of a novel sunscreen-antioxidant
FREE RADICAL RESEARCH
Autore/i: Damiani, Elisabetta; Astolfi, Paola; Cionna, L.; Ippoliti, F.; Greci, Lucedio
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/52528

Alkoxyamines of Stable Nitroxides: N-O vs C-O Bond Homolysis
HELVETICA CHIMICA ACTA
Autore/i: Gigmes, D.; Gaudel Siri, A.; Marque, S. R. A.; Bertin, D.; Tordo, P.; Astolfi, Paola; Greci, Lucedio; Rizzoli, C.
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/57621

Synthesis and Applications of a Novel Sunscreen-antioxidant
SPIN 2005 - 4th International Conference on Nitroxides Radicals; Synthesis, Properties and Implications of Nitroxides
Autore/i: Astolfi, Paola; Damiani, Elisabetta; Laura, Cionna; Francesca, Ippoliti; Lucedio, Greci
Classificazione: 4 Contributo in Atti di Convegno (Proceeding)
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/83361

Aromatic and Aliphatic Mono- and Bis-Nitroxides: a Study on Their Radical Scavengin Abilities
SPIN 2005-4th International Conference on Nitroxide Radicals: Synthesis, Properties and Implication of Nitroxides
Autore/i: Damiani, Elisabetta; Riccardo, Castagna; Astolfi, Paola; Lucedio, Greci
Classificazione: 4 Contributo in Atti di Convegno (Proceeding)
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/83362

Spin trapping of nitrogen dioxide and of radicals generated from nitrous acid
FREE RADICAL RESEARCH
Autore/i: Astolfi, Paola; Greci, Lucedio; Panagiotaki, M.
Editore: Taylor & Francis Limited:Rankine Road, Basingstoke RG24 8PR United Kingdom:011 44 1256 813035, EMAIL: madeline.sims@tandf.co.uk, info@tandf.co.uk, INTERNET: http://www.tandf.co.uk, Fax: 011 44 1256 330245
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/57613

New Mediators for the enzyme laccase: mechanistic features and selectivity in the oxidation of non phenolic-substrates
NEW JOURNAL OF CHEMISTRY
Autore/i: Astolfi, Paola; Brandi, P.; Galli, C.; Gentili, P.; Gerini, M. F.; Greci, Lucedio; Lanzalunga, O.
Editore: Royal Society of Chemistry:Thomas Graham House, Science Park, Cambridge CB4 0WF United Kingdom:011 44 1223 432360, EMAIL: sales@rsc.org, INTERNET: http://www.rsc.org, http://www.chensoc.org, Fax: 011 44 1223 423429
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/57617

Variability of biological effects of silicas: different degrees of activation of the fifth component of complement by amorphous silicas.
TOXICOLOGY AND APPLIED PHARMACOLOGY
Autore/i: Governa, Mario; Amati, Monica; Fenoglio, I.; Valentino, Matteo; Coloccini, S.; Bolognini, L.; Botta, G. C.; Emanuelli, Monica; Pierella, Francesca; Volpe, A. R.; Astolfi, Paola; Carmignani, M.; Fubini, B.
Classificazione: 1 Contributo su Rivista
Abstract: A biogenic and a pyrogenic amorphous silica were incubated in normal human plasma and compared on a per unit surface basis for their ability to split C5 molecules and yield small C5a peptides. Since C5a peptides induce selective chemotactic attraction of polymorphonuclear leukocytes (PMN), measurement of PMN-induced chemotaxis was used as an index of C5 activation. Though to a lesser extent than the crystalline forms, amorphous silicas can promote the cleavage of C5 protein and generation of C5a-like fragment. The biogenic silica, which differs from the pyrogenic variety in particle shape, level of contaminants, and degree of surface hydrophilicity, besides specific surface, induced a greater response. Both silicas activated C5 through a process which seems to involve multiple events similar to those induced by crystalline silica. C5 molecules are adsorbed and hydroxyl radicals are generated through Haber Weiss cycles catalyzed by the redox-active iron present at the particle surface either as trace impurities or chelated from plasma by silanol groups. In turn, these radicals convert native C5 to an oxidized C5-like form C5(H2O2). Finally, C5(H2O2) is cleaved by protease enzymatic action of plasma kallikrein activated by the same silica dusts, yielding a product, C5a(H2O2), having the same functional characteristic as C5a.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/50083

Aromatic and aliphatic mono- and bis-nitroxides: a study on their radical scavenging abilities
FREE RADICAL RESEARCH
Autore/i: Damiani, Elisabetta; Castagna, R.; Astolfi, Paola; Greci, Lucedio
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/52467

Effect of complexation with randomly methylated β-cyclodextrin on the aqueous solubility, photostability and antioxidant activity of an indolinonic nitroxide radical
FREE RADICAL RESEARCH
Autore/i: Damiani, Elisabetta; Tursilli, R.; Casolari, A.; Astolfi, Paola; Greci, Lucedio; Scalia, S.
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/50287

New Insights in the Reactivity of nitrogen Dioxide with Substituted Phenols. A Solvent Effect
EUROPEAN JOURNAL OF ORGANIC CHEMISTRY
Autore/i: Astolfi, Paola; Panagiotaki, M.; Greci, Lucedio
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/57615

Diarilnitrossidi da Nitrosobenzeni con Monossido e Biossido di Azoto
7° Convegno Nazionale GIRSE
Autore/i: Lucedio, Greci; Stipa, Pierluigi; Astolfi, Paola; Maria, Panagiotaki; Milvia, Marini
Classificazione: 4 Contributo in Atti di Convegno (Proceeding)
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/83364

Synthesis of a novel nitroxide antioxidant and methods of use in cosmetic and dermatological compositions
Autore/i: Damiani, Elisabetta; Astolfi, Paola; L., Greci
Classificazione: 6 Brevetti
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/48659

On the Reactivity of Nitrogen Dioxide with Substituted Phenols
ISOFR 9th-International Symposium on Organic Free Radicals
Autore/i: Astolfi, Paola; Sandro, Capancioni; Lucedio, Greci
Classificazione: 4 Contributo in Atti di Convegno (Proceeding)
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/83357

Reaction of Nitrogen Dioxide and Nitrous Acid with Indoles
ISOFR 9th-International Symposium on Organic Free Radicals
Autore/i: Astolfi, Paola; Maria, Panagiotaki; Lucedio, Greci
Classificazione: 4 Contributo in Atti di Convegno (Proceeding)
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/83358

New Insight on the Reactivity of Phenols with Nitrogen Dioxide
COFEM 2004-Giornate di Chimica Organica Fisica e Meccanicistica
Autore/i: Astolfi, Paola; Lucedio, Greci
Classificazione: 4 Contributo in Atti di Convegno (Proceeding)
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/83360

A Preliminary EPR Study on the Formation and Inhibition of Gas Hydrates in the Oil Industry
ADVANCED TECHNIQUES & APPLICATIONS OF EPR-37th Annual International Meeting of the Electron Spin Resonance Group of the Royal Society of Chemistry
Autore/i: Claudio, Carati; Luciano, Montanari; Astolfi, Paola; Lucedio, Greci
Classificazione: 4 Contributo in Atti di Convegno (Proceeding)
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/83349

Oxazoles Formation During O-Alkylation of Isonitroso-naphthols. X-Ray Structure of [1,2]Naphthoquinone 1-[O-(4-tert-Butyl-benzyl)-oxime] and 2-(4-tert-Butyl-phenyl)Napth[1,2-d]Oxazole
JOURNAL OF HETEROCYCLIC CHEMISTRY
Autore/i: Astolfi, Paola; Carloni, Patricia; Castagna, R; Greci, Lucedio; Rizzoli, C; Stipa, Pierluigi
Classificazione: 1 Contributo su Rivista
Abstract: 1-Nitroso-2-naphthol and 2-nitroso-1-naphthol, both in the isonitroso form, react with benzyl bromides in THF and in the presence of triethylamine affording, in low yields, the corresponding O-benzyl oximes and 2-aryl naphthoxazoles in a 1:1 ratio, approximately. The structures of O-benzyl oximes and naphthoxazoles isolated have been determined by X-ray analysis.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/51020

Influence of Cyclodextrins on the Aqueous Solubility, Photostability and Antioxidant Activity of Indolinonic Nitroxides Radicals
12th International Cyclodextrin Symposium
Autore/i: Santo, Scalia; Damiani, Elisabetta; Silvia, Simeoni; Astolfi, Paola; Lucedio, Greci
Classificazione: 4 Contributo in Atti di Convegno (Proceeding)
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/83353

Hydrogen abstraction ability of different aromatic nitroxides
FREE RADICAL RESEARCH
Autore/i: Damiani, Elisabetta; Astolfi, Paola; Benaglia, M.; Alberti, A.; Greci, Lucedio
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/52908

Adsorption of Spin-Labelled Hydrophilic Polymers on Quartz Sands
5th Meeting of the European Federation of EPR Groups
Autore/i: Claudio, Carati; Luciano, Montanari; Lucedio, Greci; Damiani, Elisabetta; Astolfi, Paola
Classificazione: 4 Contributo in Atti di Convegno (Proceeding)
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/83341

On the Spin Trapping of Nitroxide Dioxide
5th Meeting of the European Federation of EPR Groups
Autore/i: Astolfi, Paola; Sandro, Capancioni; Lucedio, Greci
Classificazione: 4 Contributo in Atti di Convegno (Proceeding)
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/83345

Regio- and Diasteroselectivity in 1,3-dipolar cycloaddition reactions of 2-phenylisatogen and its 3-phenylimino derivative with electron deficient alkenes. Part 2.
EUROPEAN JOURNAL OF ORGANIC CHEMISTRY
Autore/i: Astolfi, Paola; Bruni, Paolo; Greci, Lucedio; Stipa, Pierluigi; Righi, L.; Rizzoli, C.
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/52386

Hydroxylamines as Oxidation Catalysts: Thermochemical and Kinetic Studies
JOURNAL OF ORGANIC CHEMISTRY
Autore/i: Amorati, R.; Lucarini, M.; Mugnaini, V.; Pedulli, G. F.; Minisci, F.; Recupero, F.; Fontana, F.; Astolfi, Paola; Greci, Lucedio
Editore: [Washington, D.C: American Chemical Society
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/57606