Michela PISANI

Pubblicazioni

Michela PISANI

 

102 pubblicazioni classificate nel seguente modo:

Nr. doc. Classificazioni
55 1 Contributo su Rivista
39 4 Contributo in Atti di Convegno (Proceeding)
5 2 Contributo in Volume
3 5 Altro
Anno
Risorse
2024
Assessment of molecularly imprinted polymers for selective removal of diclofenac from wastewater by laboratory and pilot-scale adsorption tests
JOURNAL OF WATER PROCESS ENGINEERING
Autore/i: Parlapiano, Marco; Foglia, Alessia; Sgroi, Massimiliano; Pisani, Michela; Astolfi, Paola; Mezzelani, Marica; Gorbi, Stefania; Akyol, Çağrı; Eusebi, Anna Laura; Fatone, Francesco
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/329513 Collegamento a IRIS

2024
Macroscopic Biaxial Order in Multilayer Films of Bent-Core Liquid Crystals Deposited by Combined Langmuir–Blodgett/Langmuir–Schaefer Technique
NANOMATERIALS
Autore/i: Vita, F.; Adamo, F. C.; Campana, M.; Bordokas, B.; Ciuchi, F.; De Santo, M. P.; Hermida-Merino, D.; Lisovsky, A.; Pisani, M.; Pontoni, D.; Scharrer, E.; Francescangeli, O.
Classificazione: 1 Contributo su Rivista
Abstract: Bent-core liquid crystals, a class of mesogenic compounds with non-linear molecular structures, are well known for their unconventional mesophases, characterized by complex molecular (and supramolecular) ordering and often featuring biaxial and polar properties. In the nematic phase, their unique behavior is manifested in the formation of nano-sized biaxial clusters of layered molecules (cybotactic groups). While this prompted their consideration in the quest for nematic biaxiality, experimental evidence indicates that the cybotactic order is only short-ranged and that the nematic phase is macroscopically uniaxial. By combining atomic force microscopy, neutron reflectivity and wide-angle grazing-incidence X-ray scattering, here, we demonstrate that multilayer films of a bent-core nematic, deposited on silicon by a combined Langmuir–Blodgett and Langmuir–Schaefer approach, exhibit macroscopic in-plane ordering, with the long molecular axis tilted with respect to the sample surface and the short molecular axis (i.e., the apex bisector) aligned along the film compression direction. We thus propose the use of Langmuir films as an effective way to study and control the complex anchoring properties of bent-core liquid crystals.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/328032 Collegamento a IRIS

2023
The influence of mannose-based esters on the mesophase behaviour of lyotropic liquid crystalline nanosystems as drug delivery vectors
COLLOIDS AND SURFACES. B, BIOINTERFACES
Autore/i: Tiboni, M.; Astolfi, P.; Verboni, M.; Benedetti, S.; Giorgini, E.; Notarstefano, V.; Vita, F.; Ranieri, S.; Duranti, A.; Lucarini, S.; Casettari, L.; Pisani, M.
Classificazione: 1 Contributo su Rivista
Abstract: Lyotropic Liquid Crystalline (LLC) nanoparticles represent an emerging class of smart, biocompatible, and biodegradable systems for the delivery of drugs. Among these, structures with complex 3D architectures such as cubosomes are of particular interest. These are non- lamellar assemblies having hydrophobic and hydrophilic portions able to carry drugs of different nature. They can further be modulated including suitable additives to control the release of the active payload, and to promote an active targeting. Starting from monoolein (GMO) cubic phase, different concentrations of mannose-based esters were added, and the eventual structural modifications were monitored to ascertain the effects of the presence of glycolipids. Moreover, the structural properties of these nanosystems loaded with Dexamethasone (DEX), a very well-known anti-inflammatory steroid, were also studied. Experiments were carried out by synchrotron Small Angle X-ray Scattering (SAXS), Raman Microspectroscopy (RMS) and Attenuated Total Reflectance-Fourier Transform Infrared (ATR-FTIR) measurements. The drug delivery potential (i.e. entrapment efficiency and release properties) of the obtained nanoparticles was evaluated. Finally, in vitro cytocompatibility and anti-inflammatory activity studies of the prepared formulations were carried out. Inclusion of mannose-based surfactants up to 10 mol% influenced the structural parameters of Im3m cubic phase and swollen cubic phases were obtained with the different glycolipids with lattice parameters significantly higher than GMO. A complete cytocompatibility and an increased DEX activity were observed, thus suggesting the possibility to use GMO/glycolipids nanoparticles to formulate innovative drug delivery systems.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/326431 Collegamento a IRIS

2022
Nanoscale Structure of Langmuir–Blodgett Film of Bent-Core Molecules
NANOMATERIALS
Autore/i: Adamo, F. C.; Ciuchi, F.; De Santo, M. P.; Astolfi, P.; Warner, I.; Scharrer, E.; Pisani, M.; Vita, F.; Francescangeli, O.
Classificazione: 1 Contributo su Rivista
Abstract: Bent-core mesogens (BCMs) are a class of thermotropic liquid crystals featuring several unconventional properties. However, the interpretation and technological exploitation of their unique behavior have been hampered by the difficulty of controlling their anchoring at surfaces. To tackle this issue, we report the nanoscale structural characterization of BCM films prepared using the Langmuir–Blodgett technique. Even though BCMs are quite different from typical amphiphilic molecules, we demonstrate that stable molecular films form over water, which can then be transferred onto silicon substrates. The combination of Brewster angle microscopy, atomic force microscopy, and X-ray reflectivity measurements shows that the molecules, once transferred onto a solid substrate, form a bilayer structure with a bottom layer of flat molecules and an upper layer of upright molecules. These results suggest that Langmuir–Blodgett films of BCMs can provide a useful means to control the alignment of this class of liquid crystals.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/305903 Collegamento a IRIS

2022
A vibrational in vitro approach to evaluate the potential of monoolein nanoparticles as isofuranodiene carrier in MDA-MB 231 breast cancer cell line: new insights from Infrared and Raman microspectroscopies
SPECTROCHIMICA ACTA. PART A, MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
Autore/i: Notarstefano, Valentina; Pisani, Michela; Bramucci, Massimo; Quassinti, Luana; Maggi, Filippo; Vaccari, Lisa; Parlapiano, Marco; Giorgini, Elisabetta; Astolfi, Paola
Classificazione: 1 Contributo su Rivista
Abstract: Isofuranodiene (IFD) is a sesquiterpene occurring in several plant species, which proved to have multiple anticancer activities. IFD has a lipophilic nature and, hence, a very low water solubility and a poor bioavailability; moreover, it is not stable, undergoing the “Cope rearrangement” to the less active curzerene. The use of appropriate delivery systems can thus be considered as a valid tool to enhance IFD bioavailability, solubility, stability and at the same time also to improve its intracellular uptake and pharmacological activity. Within this frame, monoolein (GMO) nanoparticles loaded with IFD were prepared and their enhanced anticancer activity, compared to pristine IFD, was assessed. In this study, for the first time, an in vitro Fourier Transform Infrared and Raman Microspectroscopy approaches were exploited to evaluate the effects of IFD, alone and loaded in GMO nanoparticles, on MDA-MB 231 breast cancer cell line. The anti-cancer effects of IFD were evidenced by both the spectroscopic techniques and discriminated from the GMO-induced changes in the culture environment; moreover, a synergistic effect of IFD and GMO administration can be envisaged by the experimental results.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/296601 Collegamento a IRIS

2022
Intercalation or external binding: How to torque chromonic Sunset Yellow
JOURNAL OF MOLECULAR LIQUIDS
Autore/i: Spina, L.; Penelope De Santo, M.; Caterina Maria, T.; Pisani, M.; Vita, F.; Barberi, R.; Ciuchi, F.
Classificazione: 1 Contributo su Rivista
Abstract: The reflection symmetry breaking observed in chromonic liquid crystals confined in different geometries, like tactoids, microspheres or capillaries, has been the focus of researchers in the last few years. Although the assembly mechanism that drives this behavior is common to most chromonic materials, the interface phenomena and the induced chirality are strongly dependent on the chromonic molecule itself. In this work, we report advances in understanding the supramolecular arrangement and induced chirality for the chromonic molecule Sunset Yellow and its interaction with salts and L-peptides. By means of optical microscopy, X-ray diffraction and atomic force microscopy, we demonstrate the rise of enhanced chirality in Sunset Yellow microspheres and propose an explanation of this phenomenon through two possible mechanisms: intercalation of the chiral dopant or binding. Our findings on the capability to control the delicate thermodynamic balance among chromonics, ions, chiral dopants, and water and its kinetics may open new perspectives for using these microspheres in optical and sensing applications.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/305902 Collegamento a IRIS

2022
Characterisation of Bee Pollen from the Marche Region (Italy) According to the Botanical and Geographical Origin with Analysis of Antioxidant Activity and Colour, Using a Chemometric Approach
MOLECULES
Autore/i: Castiglioni, S.; Stefano, M.; Astolfi, P.; Pisani, M.; Carloni, P.
Classificazione: 1 Contributo su Rivista
Abstract: Attempts have often been made to isolate and characterise monofloral pollens to correlate nutritional with botanical properties. Nevertheless, pollen harvested in a particular area that can have a high biodiversity could have healthier properties. In addition, the analysis of the pollen's botanical composition can be important for characterising the typical flora of a specific geographical area. On this basis, various pollens collected in different locations of the Marche region (Italy) and in different harvesting periods were analyzed for botanical composition and antioxidant (total phenolic content, ABTS, DPPH and ORAC tests), granulometry and colour (CIE L*a*b*) properties to evaluate the biodiversity of pollen sources within a particular geographical area and to correlate this to the nutraceutical characteristics. Antioxidant activity results showed values generally higher than those of monofloral pollens harvested in the same areas but manually separated according to colour, shape and size. This suggests that even the floral species present in low percentages may have an influence on the nutraceutical properties of these products. The multivariate statistical elaboration of the obtained results permitted the separation of samples containing a prevalent botanical species and the grouping of all the samples into separate clusters corresponding to different areas of Marche.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/313874 Collegamento a IRIS

2021
Selective removal of contaminants of emerging concern (CECs) from urban water cycle via Molecularly Imprinted Polymers (MIPs): Potential of upscaling and enabling reclaimed water reuse
JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING
Autore/i: Parlapiano, M.; Akyol, C.; Foglia, A.; Pisani, M.; Astolfi, P.; Eusebi, A. L.; Fatone, F.
Classificazione: 1 Contributo su Rivista
Abstract: Contaminants of emerging concern (CECs) are one of the main barriers in the water cycle as they limit the water reuse due to their adverse effects on humans and the ecosystem. Natural and/or engineered ecosystems, such as conventional wastewater treatment processes, are not designed to remove CECs and contribute to the bioaccumulation in organisms considering high volumes of treated water discharges. The adoption of innovative solutions to upgrade urban water cycle facilities has gained relevance for the removal of these substances from final effluents. Molecularly imprinted polymers (MIPs) show promising selective removal toward a wide range of CECs. However, this process is still limited to lab/bench scale and needs to be critically analyzed and assessed for possible scale-up in real environment. Therefore, in this review, an overview of the fate and occurrence of CECs in wastewater is initially reported together with the state-of-the-art adsorption mechanisms to remove these compounds. In the central part of the paper, an evaluation of MIPs synthesis and their status in removing CECs from water matrix are presented. An upscaling pathway of MIPs column from lab- to pilot-scale is given to be applied for enhanced CECs removal and safe water reuse in irrigation/fertigation. Finally, possible integration of MIP columns to real wastewater treatment facilities is discussed and advantages and disadvantages of the potential solutions are addressed to enhance their sustainability.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/294626 Collegamento a IRIS

2021
Tailoring non-conventional water resources for sustainable and safe reuse in agriculture
CHEMICAL ENGINEERING TRANSACTIONS
Autore/i: Foglia, A.; Parlapiano, M.; Akyol, G. C. C.; Eusebi, A. L.; Pisani, M.; Astolfi, P.; Fatone, F.
Classificazione: 1 Contributo su Rivista
Abstract: In water scarce areas, like the Mediterranean Region where the lack of irrigation water is already limiting agricultural production, the valorization of non-concventional water resources is utmost important. Accordingly, in this study, we aimed to provide safe and locally sustainable ways of water supply for the Mediterranean agricultural sector by exploiting non-conventional water resources for irrigation purpose. In this context, pilot scale anaerobic reactors treating urban wastewater were operated coupling upflow anaerobıc sludge blanket (UASB) reactor and ultrafiltration anaerobic membrane bioreactor (AnMBR). The resulting permeate is of high quality, accomplishing the EU quality standards for irrigation water reuse, also in terms of E.coli as the main microbial indicator. However, contaminants of emerging concern (CECs) can be a further limitation for safe reuse of the reclaimed water. Hence, molecularly imprinted polymers (MIPs) were further integrated as the final refining step for the selective removal of CECs. Diclofenac was used as the target compound with a removal efficiency up to 50% in the final effluent. In parallel, an intensive pilot system was operated for brine treatment through evaporation, chemical addition and precipitation, and forward osmosis, where up to 77% Mg2+ and 45% Ca2+ recovery rates were achieved. The recovered Mg-salts from the brine treatment were then used as an external source to enhance the struvite precipitation in the N- and P-rich effluent of AnMBR. The preliminary tests showed that only hydroxiapatite salts precipitated without any external P addition.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/294627 Collegamento a IRIS

2021
Cubic and Hexagonal Mesophases for Protein Encapsulation: Structural Effects of Insulin Confinement
LANGMUIR
Autore/i: Astolfi, Paola; Giorgini, Elisabetta; Romano Perinelli, Diego; Vita, Francesco; Adamo, FABRIZIO CORRADO; Logrippo, Serena; Parlapiano, Marco; Bonacucina, Giulia; Pucciarelli, Stefania; Francescangeli, Oriano; Vaccari, Lisa; Pisani, Michela
Classificazione: 1 Contributo su Rivista
Abstract: Monoolein-based cubic and hexagonal mesophases were investigated as matrices for insulin loading, at low pH, as a function of temperature and in the presence of increasing amounts of oleic acid, as a structural stabilizer for the hexagonal phase. Synchrotron small angle X-ray diffraction, rheological measurements, and attenuated total reflection-Fourier transform infrared spectroscopy were used to study the effects of insulin loading on the lipid mesophases and of the effect of protein confinement in the 2D- and 3D-lipid matrix water channels on its stability and unfolding behavior. We found that insulin encapsulation has only little effects both on the mesophase structures and on the viscoelastic properties of lipid systems, whereas protein confinement affects the response of the secondary structure of insulin to thermal changes in a different manner according to the specific mesophase: in the cubic structure, the unfolding toward an unordered structure is favored, while the prevalence of parallel β-sheets, and nuclei for fibril formation, is observed in hexagonal structures.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/291792 Collegamento a IRIS

2021
Antioxidant Activity Level, Bioactive Compounds, Colour and Spectroscopic Analysis (UV-Vis and FT-IR) of Flavoured Drinks Made with Wine and Sour Cherries (Prunus cerasus Var. austera)
FOODS
Autore/i: Pisani, M; Astolfi, P.; Sabbatini, S.; Carloni, P.
Classificazione: 1 Contributo su Rivista
Abstract: In recent years, the increase in consumer interest towards simpler and authentic lifestyles has led to an explosive growth in the production and business of typical agri-food products and, among these, of wines and its derived beverages. With the aim of promoting a typical Italian beverage, the so-called “Vino di visciole” or “Visner”, listed in the national table of traditional agrifood products, the antioxidant and colour properties of fifteen samples from different provinces of the Marche region and obtained with different recipes were analysed. The “in vitro” total antioxidant activity (TAA) determined using ABTS assays, total phenolic content (TPC), total flavonoid content (TFC), total anthocyanins content (TAC), and colour (Somers assay) were measured. In addition, a spectroscopic FT-IR and UV-Vis analysis was carried out to analyse samples with multivariate techniques. The results showed that the production area, the recipe, and the type of cherries used to make the alcoholic beverage do not influence the antioxidant properties and the phytochemical contents of the samples. The multivariate treatment of the spectroscopic features (mainly UV-Vis) rather allowed the differentiation of samples with high antioxidant activity using easy and low-cost instrumental techniques that require little time and can be employed in routine analysis.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/291762 Collegamento a IRIS

2020
Nanostructure of Unconventional Liquid Crystals Investigated by Synchrotron Radiation
NANOMATERIALS
Autore/i: Vita, Francesco; Adamo, Fabrizio Corrado; Pisani, Michela; Francescangeli, Oriano
Classificazione: 1 Contributo su Rivista
Abstract: The macroscopic properties of novel liquid crystal (LC) systems—LCs with unconventional molecular structure as well as conventional LCs in unconventional geometries—directly descend from their mesoscopic structural organization. While X-ray diffraction (XRD) is an obvious choice to investigate their nanoscale structure, conventional diffractometry is often hampered by experimental difficulties: the low scattering power and short-range positional order of the materials, resulting in weak and diffuse diffraction features; the need to perform measurements in challenging conditions, e.g., under magnetic and/or electric fields, on thin films, or at high temperatures; and the necessity to probe micron-sized volumes to tell the local structural properties from their macroscopic average. Synchrotron XRD allows these problems to be circumvented thanks to the superior diffraction capabilities (brilliance, q-range, energy and space resolution) and advanced sample environment available at synchrotron beamlines. Here, we highlight the potentiality of synchrotron XRD in the field of LCs by reviewing a selection of experiments on three unconventional LC systems: the potentially biaxial and polar nematic phase of bent-core mesogens; the very high-temperature nematic phase of all-aromatic LCs; and polymer-dispersed liquid crystals. In all these cases, synchrotron XRD unveils subtle nanostructural features that are reflected into macroscopic properties of great interest from both fundamental and technological points of view.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/284390 Collegamento a IRIS

2020
Nanostructured liquid crystalline particles as delivery vectors for isofuranodiene: Characterization and in-vitro anticancer activity
COLLOIDS AND SURFACES. B, BIOINTERFACES
Autore/i: Pisani, M.; Quassinti, L.; Bramucci, M.; Galassi, R.; Maggi, F.; Rossi, B.; Damin, A.; Carloni, P.; Astolfi, P.
Classificazione: 1 Contributo su Rivista
Abstract: Isofuranodiene is an oxygenated sesquiterpene containing a furan ring isolated from the essential oil of Smyrnium olusatrum L. (Apiaceae) owning notable anticancer activity. Despite its biological potential, the high lipophilicity along with a relatively low stability due to Cope rearrangement giving rise to a less active compound, make the perspective of its therapeutical use unlikely. On this basis, in the present work we evaluated bulk and dispersed non lamellar liquid crystalline phases as effective delivery vectors for isofuranodiene, and capable of preserving its structure and enhancing the biological activity. Small-angle X-ray scattering, dynamic light scattering, and UV resonance Raman spectroscopy were used to characterize the nanosystems in an integrated experimental approach. Encapsulation of isofuranodiene in the lipid matrix resulted in a transition from a cubic Im3m to a reversed hexagonal phase because of the highly lipophilic character of the drug, as obtained in SAXS measurements, and in significant shifts in the components of the Raman spectrum of isofuranodiene. The anticancer activity of isofuranodiene-loaded lipidic nanoparticles was assessed on MDA-MB 231 cell line by MTT assay and was found to be higher than that of pristine isofuranodiene.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/277931 Collegamento a IRIS

2020
Synchrotron characterization of hexagonal and cubic lipidic phases loaded with azolate/phosphane gold(I) compounds: a new approach to the uploading of gold(I)-based drugs
NANOMATERIALS
Autore/i: Astolfi, Paola; Pisani, Michela; Giorgini, Elisabetta; Rossi, Barbara; Damin, Alessandro; Vita, Francesco; Francescangeli, Oriano; Luciani, Lorenzo; Galassi, Rossana
Classificazione: 1 Contributo su Rivista
Abstract: Gold(I) phosphane compounds have recently attracted a renewed interest as potential new protagonists in cancer therapy. A class of phosphane gold(I) complexes containing azolate ligands has been successfully tested against several cancer cell lines and, in particular, against basal-like breast (BLB) cancer, a form characterized by strongly severe diagnosis and short life lapse after classic chemotherapy. Even though the anticancer activity of gold(I) phosphane compounds is thoroughly ascertained, no study has been devoted to the possibility of their delivery in nanovectors. Herein, nonlamellar lyotropic liquid crystalline lipid nanosystems, a promising class of smart materials, have been used to encapsulate gold(I) azolate/phosphane complexes. In particular, ((triphenylphosphine)-gold(I)-(4,5-dichloroimidazolyl-1H-1yl)) (C-I) and ((triphenylphosphine)-gold(I)-(4,5-dicyanoimidazolyl-1H-1yl)) (C-II) have been encapsulated in three different lipid matrices: monoolein (GMO), phytantriol (PHYT) and dioleoyl-phosphatidylethanolamine (DOPE). An integrated experimental approach involving X-ray diffraction and UV resonant Raman (UVRR) spectroscopy, based on synchrotron light and attenuated total reflectance Fourier transform infrared (ATR-FTIR) spectroscopy, has been employed to establish the effects of drug encapsulation on the structure and phase behavior of the host mesophases. The results indicate that gold(I) complexes C-I and C-II are successfully encapsulated in the three lipid matrices as evidenced by the drug-induced phase transitions or by the changes in the mesophase lattice parameters observed in X-ray diffraction experiments and by the spectral changes occurring in UV resonant Raman spectra upon loading the lipid matrices with C-I and C-II.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/284396 Collegamento a IRIS

2020
Liquid crystal thermosets. A new class of high-performance materials
LIQUID CRYSTALS
Autore/i: Vita, Francesco; Adamo, Fabrizio C.; Pisani, Michela; Heist, Leah M.; Li, Ming; Hegde, Maruti; Dingemans, Theo J.; Samulski, Edward T.; Francescangeli, Oriano
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/267946 Collegamento a IRIS

2020
Biaxial ordering in the supercooled nematic phase of bent-core mesogens: effects of molecular symmetry and outer wing lateral groups
LIQUID CRYSTALS
Autore/i: Harkins, Robin; Tauscher, Tatum; Nguyen, Jason; Lewis, Sunny; Adamo, Fabrizio C.; Pisani, Michela; Hermida-Merino, Daniel; Samulski, Edward T.; Vita, Francesco; Francescangeli, Oriano; Scharrer, Eric
Classificazione: 1 Contributo su Rivista
Abstract: In previous research, we have found that the incorporation of three lateral substituents on bentcore oxadiazole-based liquid crystals has allowed for the presence of a nematic phase that supercooled to room temperature. X-ray diffraction (XRD) studies of these derivatives have confirmed that the nematic phase consists of cybotactic clusters. Moreover, the wide-angle XRD pattern showed a splitting indicative of local biaxial ordering within this phase. In this paper, we report the phase behaviour of two series of compounds that contain up to four lateral methyl groups. In one series, the arrangement of the lateral groups is symmetrical, and in the other case, the groups are not symmetrically distributed. All of the non-symmetric derivatives supercool to room temperature in the nematic phase, while symmetric derivatives tend to crystallise well above room temperature. XRD analysis of all of these compounds indicates the presence of cybotactic order. However, a splitting of the wide-angle XRD pattern is observed only in derivatives with lateral groups on the outer benzene rings, regardless of their supercooling behaviour, indicating the crucial influence of these substituents on the molecular packing.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/267952 Collegamento a IRIS

2020
Investigation of human pancreatic cancer tissues by Fourier Transform Infrared Hyperspectral Imaging
JOURNAL OF BIOPHOTONICS
Autore/i: Notarstefano, Valentina; Sabbatini, Simona; Conti, Carla; Pisani, Michela; Astolfi, Paola; Pro, Chiara; Rubini, Corrado; Vaccari, Lisa; Giorgini, Elisabetta
Classificazione: 1 Contributo su Rivista
Abstract: Fourier-transform infrared hyperspectral imaging (FTIR-HSI) provides hyperspectral images containing both morphological and chemical information. It is widely applied in the biomedical field to detect tumor lesions, even at the early stage, by identifying specific spectral biomarkers. Pancreatic neoplasms present different prognoses and are not always easily classified by conventional analyses. In this study, tissue samples with diagnosis of pancreatic ductal adenocarcinoma and pancreatic neuroendocrine tumor were analyzed by FTIR-HSI and the spectral data compared with those from healthy and dysplastic samples. Multivariate/univariate approaches were complemented to hyperspectral images, and definite spectral markers of the different lesions identified. The malignant lesions were recognizable both from healthy/dysplastic pancreatic tissues (high values of phospholipids and triglycerides with shorter, more branched and less unsaturated alkyl chains) and between each other (different amounts of total lipids, phosphates and carbohydrates). These findings highlight different metabolic pathways characterizing the different samples, well detectable by FTIR-HSI.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/272050 Collegamento a IRIS

2019
50 Years of Chemistry in the Engineering Faculty: From Free Radicals to Nanosystems
The First Outstanding 50 Years of “Università Politecnica delle Marche” Research Achievements in Physical Sciences and Engineering
Autore/i: Stipa, Pierluigi; Sabbatini, Simona; Pisani, Michela; Cardellini, Liberato
Classificazione: 2 Contributo in Volume
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/278702 Collegamento a IRIS

2019
Encapsulation of vitamin B12 into nanoengineered capsules and soft matter nanosystems for targeted delivery
COLLOIDS AND SURFACES. B, BIOINTERFACES
Autore/i: Maiorova, L. A.; Erokhina, S. I.; Pisani, M.; Barucca, G.; Marcaccio, M.; Koifman, O. I.; Salnikov, D. S.; Gromova, O. A.; Astolfi, P.; Ricci, V.; Erokhin, V.
Classificazione: 1 Contributo su Rivista
Abstract: Targeted delivery of vitamins to a desirable area is an active branch in a modern pharmacology. The most important and difficult delivery of vitamin B12 is that to bone marrow and nerve cells. Herein we present a first step towards the development of two types of smart carriers, polymer capsules and lyotropic liquid-crystalline nanosystems, for vitamin B12 targeted delivery and induced release. A vitamin B12 encapsulation technique into nanoengineered polymeric capsules produced by layer-by-layer assembling of polymeric shells on CaCO3 templates has been developed. The effectiveness of the process was demonstrated by optical absorption spectroscopy, transmission electron microscopy (TEM), atomic force microscopy (AFM) and small-angle X-ray diffraction. TEM and AFM analyses performed on capsules after their drying, confirmed the presence of the vitamin B12 inside the capsules in the form of crystalline nanoaggregates, 50–300 nm in diameter. Soft lipid nanovectors consisting of amphiphilic phytantriol molecules, which in water excess spontaneously self-assembly in 3D wellordered inverse bicontinuous cubic bulk phase, were used as alternative carriers for vitamin B12. It was shown that about 30% of the vitamin added in the preparation of the soft lipid system was actually encapsulated in cubosomes and that no structural changes occurred upon loading. The Vitamin stabilizes the lipid system playing the role of its structure-forming element. The biocompatible nature, the stability and the feasibility of these systems make them good candidates as carriers for hydrophilic vitamins.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/269787 Collegamento a IRIS

2019
Effects of a cationic surfactant incorporation in phytantriol bulk cubic phases and dispersions loaded with the anticancer drug 5-fluorouracil
JOURNAL OF MOLECULAR LIQUIDS
Autore/i: Astolfi, P.; Giorgini, E.; Adamo, F. C.; Vita, F.; Logrippo, Serena; Francescangeli, O.; Pisani, M.
Classificazione: 1 Contributo su Rivista
Abstract: Ordered lyotropic liquid crystalline phases, which are particularly interesting for several biological and biomedical applications, can show the advantage of having a tunable three-dimensional nanostructure. Addition of ionic surfactants to lipid/water binary systems may influence their phase behavior and their structural properties, such as bilayer thickness, water channel diameter and interfacial curvature, resulting in specific amphiphile self-assembly materials. Understanding the effects of such additives is thus of considerable interest and, in this context, we studied the incorporation of a cationic surfactant (didodecyldimethylammonium bromide) in the phytantriol cubic phase, unloaded and loaded with the anticancer drug 5-fluorouracil. In particular, an anionic form of the drug was also used which could further influence the mesophase structure of the lipid matrix and its entrapment efficiency. The formulations, prepared as bulk and dispersed phases, were characterized by synchrotron small angle X-ray scattering, attenuated total reflection Fourier transform infrared and UV–Vis spectroscopies and dynamic light scattering. Addition of increasing amounts of the cationic surfactant resulted in phase changes following the sequence Pn3m-Im3m-Lα, which was almost unaltered when the drug in its neutral form was encapsulated in the systems. On the contrary, the presence of the anionic 5-fluorouracil stabilized the Pn3m cubic phase at all the cationic surfactant concentrations used.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/267942 Collegamento a IRIS

2018
Geographical characterisation of multifloral honeys from the Marche region (Italy) according to their antioxidant activity and colour using a chemometric approach
INTERNATIONAL JOURNAL OF FOOD SCIENCE & TECHNOLOGY
Autore/i: Castiglioni, Sara; Stefano, Mariassunta; Pisani, Michela; Carloni, Patricia
Classificazione: 1 Contributo su Rivista
Abstract: Multifloral honeys produced in the Marche region (Italy) were analysed leading to the antioxidant and colour characterisation of this type of regional honeys: the antioxidant activity was determined measuring total phenolic content (TPC), and using ABTS and DPPH assays. The elaboration of data with multivariate analyses enabled differentiation of the geographical origin of honey samples, namely as coming from the Mid/Coastal Hilly or from the High Hilly/Submountain belt of the Marche region. The cluster analysis applied to a selection of samples divided honeys belonging to the Mid/Coastal Hilly belt into three groups showing low, medium and high antioxidant activity and in part located in different areas of the region. Finally, using data of unifloral honeys from the Marche region previously determined, it was possible to have an indication of the prevalent nectar contained in most of the multifloral honey samples harvested in 2012 in the Marche region.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/254650 Collegamento a IRIS

2018
Selective induction of apoptosis in MCF7 cancer-cell by targeted liposomes functionalised with mannose-6-phosphate
JOURNAL OF DRUG TARGETING
Autore/i: Minnelli, Cristina; Cianfruglia, Laura; Laudadio, Emiliano; Galeazzi, Roberta; Pisani, Michela; Crucianelli, Emanuela; Bizzaro, Davide; Armeni, Tatiana; Mobbili, Giovanna
Classificazione: 1 Contributo su Rivista
Abstract: Liposomes are versatile platforms to carry anticancer drugs in targeted drug delivery; they can be surface modified by different strategies and, when coupled with targeting ligands, are able to increase cellular internalisation and organelle-specific drug delivery. An interesting strategy of antitumoral therapy could involve the use of lysosomotropic ligand-targeted liposomes loaded with molecules, which can induce lysosomal membrane permeabilization (LMP), leakage of cathepsins into the cytoplasm and subsequent apoptosis. We have previously demonstrated the ability of liposomes functionalised with a mannose-6-phosphate to reach lysosomes; in this research we compare the behaviour of M6P-modified and non-functionalised liposomes in MCF7 tumour cell and in HDF normal cells. With this aim, we first demonstrated by Western blotting the overexpression of mannose-6-phosphate/insulin-like growth factor (M6P/IGF-II) receptor in MCF7. Then, we prepared calcein-loaded liposomes and we revealed the increased uptake of M6P-functionalised liposomes in MCF7 cells respect to HDF cells by flow cytometry analysis. Finally, we loaded functionalised and not functionalised liposomes with N-hexanoyl-d-erythro-sphingosine (C6Cer), able to initiate LMP-induced apoptosis; after having studied the stability of both vesicles in the presence of serum by Dynamic Light Scattering and Spectrophotometric turbidity measurements, we showed that ceramide-loaded M6P-liposomes significantly increased apoptosis in MCF7 with respect to HDF cells. © 2017 Informa UK Limited, trading as Taylor & Francis Group
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/251684 Collegamento a IRIS

2017
Lyotropic Liquid-Crystalline Nanosystems as Drug Delivery Agents for 5-Fluorouracil: Structure and Cytotoxicity
LANGMUIR
Autore/i: Astolfi, Paola; Giorgini, Elisabetta; Gambini, Valentina; Rossi, Barbara; Vaccari, Lisa; Vita, Francesco; Francescangeli, Oriano; Marchini, Cristina; Pisani, Michela
Classificazione: 1 Contributo su Rivista
Abstract: Lyotropic cubic liquid-crystalline systems have received increasing attention due to their unique microstructural and physicochemical properties as efficient nanocarriers for drug delivery. We report the preparation and characterization of bulk phases and cubosome dispersions of phytantriol loaded with the anticancer drug 5-fluorouracil, in neutral and anionic forms. In both cases, a Pn3m cubic phase was observed. The phytantriol phase behavior can be influenced by the addition of ionic agents, and, to this purpose, a positively charged lipid, such as N-[1-(2,3-dioleoyloxy)propyl]-N,N,N-trimethylammonium chloride salt (DOTAP), was included in the studied formulations. It was found to induce a variation of the spontaneous membrane curvature of the phytantriol lipid bilayer, generating a transition from the Pn3m to the Im3m cubic phase. When 5-fluorouracil, in its anionic form (5-FUs), was encapsulated in these latter systems, a further transition to the HII hexagonal phase was observed as a consequence of the formation of a complex phytantriol/DOTAP/5-FUs. The physicochemical characterization was performed with various complementary techniques including synchrotron small-angle X-ray scattering, dynamic light scattering, and attenuated total reflection Fourier transform infrared and UV resonance Raman spectroscopies. Encapsulation of 5-fluorouracil in the corresponding nanodispersions was evaluated, and their in vitro cytotoxicity was assessed in MDA-MB-231 cell line. Phytantriol cubosomes containing 5-fluorouracil showed a higher toxicity compared with the bare drug solution, and hence they represent potential nanocarriers in the delivery of 5-fluorouracil for cancer therapy.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/251565 Collegamento a IRIS

2017
Electrodeposition of Zn-Ni-ZrO2, Zn-Ni-Al2O3 and Zn-Ni-SiC nanocomposite coatings from an alkaline bath
INTERNATIONAL JOURNAL OF ELECTROCHEMICAL SCIENCE
Autore/i: Roventi, Gabriella; Giuliani, Giampaolo; Pisani, Michela; Bellezze, Tiziano
Classificazione: 1 Contributo su Rivista
Abstract: Zn-Ni-ZrO2, Zn-Ni-Al2O3 and Zn-Ni-SiC nanocomposite coatings were obtained on mild steel substrate by electrodeposition technique from an alkaline bath. ZrO2, Al2O3 and SiC nanoparticles were added to the bath with concentrations of 5 and 10 g dm-3. The effect of current density and particles concentration on the deposit composition, morphology, structure and microharness was studied. The results show that the addition of nanoparticles to the plating solution does not affect significantly Zn-Ni electrodeposition process and alloy Ni percentage. X-ray diffraction patterns of the coatings show only the presence of cubic γ-phase Ni5Zn21. With ZrO2 or Al2O3 nanoparticles, homogeneous and compact deposits with low grain size were obtained. On the contrary, SiC nanoparticles incorporation perturbs the growth of the alloy matrix by reducing γ-phase orientation. ZrO2 and Al2O3 lead to an increase in the coating microhardness, whereas SiC leads to a decrease, due to the strong modification induced in the alloy structure.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/245745 Collegamento a IRIS

2017
Trans-cis photoisomerization in nanostructured floating layers and x-ray diffraction study of Langmuir-Schaefer films of nonyloxypheylazocinnamic acid
MOLECULAR CRYSTALS AND LIQUID CRYSTALS
Autore/i: Pisani, Michela; Maiorova, L. A.; Francescangeli, Oriano; Fokin, D. M. S.; Nikitin, K. S.; Burmistrov, V. A.; Kuvshinova, S. A.; Mengucci, Paolo; Koifman, O. I.
Classificazione: 1 Contributo su Rivista
Abstract: Azo-functionalised materials are of special interest due to their photochromic nature, i.e. reversible trans–cis isomerisation upon photoirradiation. The structure and properties of Langmuir monolayers of nonyloxyphenylazocinnamic acid (NOPACA) with and without exposure to UV-irradiation were studied. The NOPACA floating layer structure was determined by using a quantitative method of compression isotherm analysis. The results showed that the structure of two dimensional face-on M-nanoaggregates, the stable structural elements of floating monolayers, changes when exposed to UV-irradiation. Langmuir-Schaefer films of NOPACA were prepared by deposition of floating layers and studied using X-ray diffraction technique.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/250593 Collegamento a IRIS

2016
Liposomi funzionalizzati con mannosio-6-fosfato come Targeted Delivery Systems (TDS)”
Conference Proceedings
Autore/i: Minnelli, Cristina; Armeni, Tatiana; Cianfruglia, Laura; Crucianelli, Emanuela; Galeazzi, Roberta; Laudadio, Emiliano; Mobbili, Giovanna; Pisani, Michela; Stipa, Pierluigi
Classificazione: 4 Contributo in Atti di Convegno (Proceeding)
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/249735 Collegamento a IRIS

2015
Liponitroxides: EPR study and their efficacy as antioxidants in lipid membranes
RSC ADVANCES
Autore/i: Mobbili, Giovanna; Crucianelli, Emanuela; Barbon, Antonio; Marcaccio, Massimo; Pisani, Michela; Dalzini, Annalisa; Ussano, Eleonora; Bortolus, Marco; Stipa, Pierluigi; Astolfi, Paola
Classificazione: 1 Contributo su Rivista
Abstract: A series of lipid-functionalized nitroxides having a pyrroline nitroxide moiety linked either to a glycerol or to a steroid unit has been synthesized, and their inclusion inside phospholipid bilayers has been investigated by Electron Paramagnetic Resonance (EPR) spectroscopy. The antioxidant behavior of these nitroxides has been studied in azo-initiator induced lipid peroxidation by means of the Thiobarbituric Acid Reactive Species (TBARS) assay; a correlation with their penetration depth within the bilayer has been found. The possible mechanisms involved in the antioxidant action have been considered, discussed and alternative pathways have been suggested for the synthesized liponitroxides due to their different localization. The steroid derivative is limited to scavenging radicals that are generated in the aqueous phase, while the glycerolipids can also act as chain breaking antioxidants.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/230125 Collegamento a IRIS

2015
Liposome-based gene delivery systems containing a steroid derivative: computational and small angle X-ray diffraction study
RSC ADVANCES
Autore/i: Galeazzi, Roberta; Bruni, Paolo; Crucianelli, Emanuela; Laudadio, Emiliano; Marini, Milvia; Massaccesi, Luca; Mobbili, Giovanna; Pisani, Michela
Classificazione: 1 Contributo su Rivista
Abstract: In this study, the structural properties and the phase behaviour of mixed composition neutral liposomes containing a functionalized steroid are reported. With the aim to design neutral liposomes able to coordinate cations and to complex DNA, we synthesized cholesteryl-2-(picolinamido)-phenylcarbamate (CHOLp) containing an N-aryl picolinamide group as chelating agent linked to the steroid structure via a carbamate moiety. The phase behaviour of mixtures of the functionalized cholesterol (CHOLp) and dioleoyl-phosphatidylcholine (DOPC) was investigated by means of X-ray diffraction. Simultaneously, atomistic molecular dynamics simulations of DOPC/CHOLp bilayers as a function of CHOLp molar fractions were carried out to investigate the specific effects of the polar steroid on the structural and dynamic properties of these zwitterionic bilayers. The molecular modelling studies have been performed both in absence and in presence of bivalent cations salts in order to assess the CHOLp ability to coordinate metal ions. The results show good stability of the resulting DOPC/CHOLp bilayers which is improved by the presence of salt. This is particularly evident at low amount of CHOLp where a high order of the lipid tails can be observed, suggesting stabilization of the corresponding DOPC liposomes. This feature can be ascribed to the polar nature and structural properties of the ligand. In fact, due to the presence of the aromatic moieties, CHOLp combines two different behaviours, namely a propensity to realize both intermolecular π-stacking interactions and cation–π bonding mainly evident in CaCl2. The last feature confirms for CHOLp a role as a cation-mediated complexation agent for DNA. The X-ray diffraction data on the capability of DOPC/CHOLp liposomes to complex DNA was also reported.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/228347 Collegamento a IRIS

2014
Liposomes containing mannose-6-phosphatecholesteryl conjugates for lysosome-specific delivery
RSC ADVANCES
Autore/i: Crucianelli, Emanuela; Bruni, Paolo; Frontini, Andrea; Massaccesi, Luca; Pisani, Michela; Smorlesi, Arianna; Mobbili, Giovanna
Classificazione: 1 Contributo su Rivista
Abstract: Lysosomes are promising targets for cancer and enzyme replacement therapy. In this area of interest we present a novel liposomal nanocarrier containing mannose 6-phosphate-cholesteryl conjugates and show its ability to reach the lysosomes by means of confocal and fluorescence microscopy measurements
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/224780 Collegamento a IRIS

2013
An X-ray diffraction study of complexes of DNA and lactosyl-functionalised liposomes induced by bivalent metal cations: coexistence of different symmetries
LIQUID CRYSTALS
Autore/i: Pisani, Michela; Mobbili, Giovanna; Marini, Milvia; Francescangeli, Oriano; Mori, Valerio; Bruni, Paolo
Classificazione: 1 Contributo su Rivista
Abstract: By using synchrotron X-ray diffraction we studied the structure and phase behaviour of complexes of DNA with the anionic 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-lactosyl (L-PE), mixed with zwitterionic lipids 1,2-dioleoyl-sn-glycero 3-phosphocholine (DOPC) or 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE), in aqueous solutions of Mn2+ and Ca2+. Aqueous dispersions of simple L-PE/DOPC and L-PE/DOPE in the presence and absence of metal cations were also studied. All the systems complexed in a self-assembled manner, the DNA condensation being promoted by the metal cations. These complexes are organised in a variety of structures showing a number of symmetries, sometimes coexisting, for example condensed multilamellar, Lαc, inverted hexagonal, HIIc, and cubic, Pn3m. Phase distribution depends on the molar ratios between L-PE and DOPC or DOPE, respectively.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/164742 Collegamento a IRIS

2013
Benzoxazinic nitrones and nitroxides as possible antioxidants in biological systems
RSC ADVANCES
Autore/i: Astolfi, Paola; Carloni, Patricia; Marini, MARIA GIOVANNA; Mobbili, Giovanna; Pisani, Michela; Stipa, Pierluigi
Classificazione: 1 Contributo su Rivista
Abstract: Benzoxazinic nitrones and nitroxides are good antioxidants in model biological systems (micelles, lipoproteins, proteins, liposomes, sugars). With nitrones, this activity may be attributed to their radical trapping ability, whereas with nitroxides to their radical scavenging properties. In both cases, H-abstraction from the C-2 position of the benzoxazine moiety cannot be ruled out and could, at least in part, account for their antioxidant behaviour. Appropriate DFT calculations have been carried out to better understand the mechanisms of action of the studied compounds.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/136265 Collegamento a IRIS

2013
Neutral liposomes containing crown ether-lipids as potential DNA vectors
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
Autore/i: Angelini, G.; Pisani, Michela; Mobbili, Giovanna; Marini, M.; Gasbarri, C.
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/161303 Collegamento a IRIS

2012
Synthesis and Antioxidant Activity of Nitroxide Functionalized Lipids
XI Convegno Nazionale GIRSE & 1st Joint Meeting ARPE-GERPE-GIRSE
Autore/i: Astolfi, Paola; Carloni, Patricia; Mobbili, Giovanna; Petrocchi, C.; Pisani, Michela; Stipa, Pierluigi
Editore: Anna Longo e Maurizio Marrale
Classificazione: 4 Contributo in Atti di Convegno (Proceeding)
Abstract: Liposomes are biodegradable and nonimmunogenic vesicular structures made of amphipatic phospholipids which can encapsulate both hydrophilic and hydrophobic materials; because of their versatility they are used as carriers in drugs and gene delivery systems. Polyunsaturated fatty acids present in the bilayer are susceptible to oxidative damage which can alter some important properties like fluidity and permeability and can cause system delivery destruction. The mechanism of lipids oxidative degradation may involve free radical reactions that lead to changes in the unsaturated systems present and eventually to the degradation of the lipid chains. In order to maintain their physical, chemical and functional properties, liposomes need to be protected by antioxidant compounds that can delay or prevent oxidation upon reaction with radical species. Nitroxide radicals (paramagnetic compounds with the unpaired electron mainly localized on the N-O group) are known to protect biological system against oxidative processes in different media and under different stress conditions. [1] As part of our ongoing research in the field of nitrones [2] and nitroxides [3] as potential antioxidants we synthesized neutral non zwitterionic lipids labeled by nitroxide able to react with radical species. Multilamellar liposomes were prepared by mixing the new lipids with Phosphatidylcholine and their EPR features and antioxidant activity was studied.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/80538 Collegamento a IRIS

2012
Influence of functionalized lipids on the structural properties and on the ability to condense DNA of zwitterionic liposomes.
VIII Convegno Nazionale AICIng
Autore/i: Pisani, Michela; Mobbili, Giovanna; M., Marini; P., Bruni
Classificazione: 4 Contributo in Atti di Convegno (Proceeding)
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/83745 Collegamento a IRIS

2012
Synthesis, structural characterization and molecular dynamics of neutral liposomes able to complex DNA
XXXIV Convegno della Divisione di Chimica Organica "SCI 2012, Pavia"
Autore/i: Galeazzi, Roberta; Massaccesi, L; Marini, M; Mobbili, Giovanna; Pisani, Michela
Classificazione: 4 Contributo in Atti di Convegno (Proceeding)
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/82824 Collegamento a IRIS

2012
Synthesis of a mannose-6-phosphate-cholesteryl conjugate for the preparation of new drug delivery vectors .
Convegno Nazionale della divisione di chimica dei sistemi biologici
Autore/i: E., Crucianelli; M., Marini; Mobbili, Giovanna; Pisani, Michela; A., Smorlesi
Classificazione: 4 Contributo in Atti di Convegno (Proceeding)
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/83747 Collegamento a IRIS

2012
New Nitroxide functionalized Lipids with Antioxidant Activity
VIII Convegno Nazionale AICIng - Atti del Convegno
Autore/i: C., Petrocchi; Astolfi, Paola; Carloni, Patricia; Mobbili, Giovanna; Pisani, Michela; Stipa, Pierluigi
Editore: Edi-Ermes
Classificazione: 4 Contributo in Atti di Convegno (Proceeding)
Abstract: A new class of different lipids bearing a nitroxide radical moiety was synthesized and used in the preparation of multilamellar phosphatidylcholine liposomes. Analysis of the corresponding EPR spectra allowed to get information about the distribution of these free radicals within the liposome structure, and the resulting antioxidant activity was measured by means of the TBARS assay. A correlation between the functionalised lipids antioxidant activity, their dipolar moment (computed by DFT) and their EPR features could be envisaged.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/80089 Collegamento a IRIS

2012
Lipids as potential vectors for genetic materials: synthesis and characterization
Convegno Nazionale della divisione di chimica dei sistemi biologici
Autore/i: M., Marini; E., Crucianelli; Mobbili, Giovanna; Pisani, Michela
Classificazione: 4 Contributo in Atti di Convegno (Proceeding)
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/83746 Collegamento a IRIS

2012
Neutral liposomes able to complex DNA: a combined experimental and computational study
ABSTRACTS Nanomedicine: : from molecules to diagnosis and therapy
Autore/i: Galeazzi, Roberta; Marini, M; Massaccesi, L; Mobbili, Giovanna; Pisani, Michela
Classificazione: 4 Contributo in Atti di Convegno (Proceeding)
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/82823 Collegamento a IRIS

2011
Synthesis and Antioxidant Properties of Benzoxanic Nitrones and Nitroxides
Autore/i: Mobbili, Giovanna; Astolfi, Paola; Carloni, Patricia; M. G., Marini; Pisani, Michela; Stipa, Pierluigi
Luogo di pubblicazione: Lecce
Classificazione: 5 Altro
Abstract: Cominicazione Scientifica al XXIV Congresso Nazionale della Società Chimica Italiana, Lecce (Italy)
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/62372 Collegamento a IRIS

2011
Antioxidant effect of nitroxide functionalized lipids in liposomes peroxidation
XXIV Congresso Nazionale della Società Chimica Italiana
Autore/i: Pisani, Michela; Astolfi, Paola; Carloni, Patricia; Mobbili, Giovanna; C., Petrocchi; Stipa, Pierluigi
Classificazione: 4 Contributo in Atti di Convegno (Proceeding)
Abstract: Cominicazione Scientifica al XXIV Congresso Nazionale della Società Chimica Italiana, Lecce (Italy)
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/83390 Collegamento a IRIS

2011
Benzoxazinic Nitrones and Nitroxides as Antioxidants in Biological Systems
6th International Conference on Nitroxide Radicals “SPIN 2011”
Autore/i: Astolfi, Paola; Carloni, Patricia; M. G., Marini; Mobbili, Giovanna; Pisani, Michela; Stipa, Pierluigi
Luogo di pubblicazione: Marseille
Classificazione: 4 Contributo in Atti di Convegno (Proceeding)
Abstract: Comunicazione Scientifica al 6th International Conference on Nitroxide Radicals "SPIN 2011" - Marseille (France)
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/62375 Collegamento a IRIS

2011
Neutral liposomes and DNA transfection
Non-viral Gene Therapy
Autore/i: Pisani, Michela; Mobbili, Giovanna; Bruni, P.
Editore: INTECH
Classificazione: 2 Contributo in Volume
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/58349 Collegamento a IRIS

2011
New neutral liposomal delivery system for genetic material.
XXIV Congresso Nazionale della Società Chimica Italiana
Autore/i: Pisani, Michela; Mobbili, Giovanna; M., Marini; P., Bruni
Classificazione: 4 Contributo in Atti di Convegno (Proceeding)
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/83741 Collegamento a IRIS

2011
Synthesis and Antioxidant Properties of Benzoxanic Nitrones and Nitroxides
XXIV Congresso Nazionale della Società Chimica Italiana
Autore/i: Mobbili, Giovanna; Astolfi, Paola; Carloni, Patricia; M. G., Marini; Pisani, Michela; Stipa, Pierluigi
Classificazione: 4 Contributo in Atti di Convegno (Proceeding)
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/83391 Collegamento a IRIS

2011
Biophysical Characterization of Complexes of DNA with Mixtures of the Neutral Lipids 1,2-Dioleoyl-sn-glycero-3-phosphoethanolamine-N-hexanoylamine or 1,2-Dioleoyl-sn-glycero-3-phosphoethanolamine-N-dodecanoylamine and 1,2-Dioleoyl-sn-glycero-3-phosphocholinein the Presence of Bivalent Metal Cations for DNA Transfection
JOURNAL OF PHYSICAL CHEMISTRY. B, CONDENSED MATTER, MATERIALS, SURFACES, INTERFACES & BIOPHYSICAL
Autore/i: Pisani, Michela; Mobbili, Giovanna; I. F., Placentino; A., Smorlesi; P., Bruni
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/63220 Collegamento a IRIS

2011
Can Neutral Liposomes be Considered as Genetic Material Carriers for Human Gene Therapy?
MINI-REVIEWS IN ORGANIC CHEMISTRY
Autore/i: Bruni, Paolo; Francescangeli, Oriano; Marini, Milvia; Mobbili, Giovanna; Pisani, Michela; Smorlesi, Arianna
Classificazione: 1 Contributo su Rivista
Abstract: Among the synthetic vectors exploitable in Human Gene Therapy (HGT), complexes of DNA with cationic liposomes (lipoplexes) are considered the best candidates. Some degree of cytotoxicity and a limited stability on serum are still limiting factors for applications in vivo. On the contrary the corresponding complexes of neutral liposomes are enough stable and non toxic. This review has the aim to offer a survey of existing data, namely structural properties, on neutral liposomes from the point of view of HGT; and to compare, whenever possible, the different features of the two classes of compounds. The outlook is that in near future neutral liposomes could behave as efficient and safe vectors of genetic material.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/58329 Collegamento a IRIS

2010
Synthesis and characterization of new synthetic vectors of DNA for human gene therapy applications
XXXIII Convegno Nazionale della Divisione di Chimica Organica Società Chimica Italiana
Autore/i: Mobbili, Giovanna; Bruni, P.; Marini, M.; Pisani, Michela; Smorlesi, A.
Classificazione: 5 Altro
Abstract: XXXIII Convegno Nazionale della Divisione di Chimica Organica Società Chimica Italiana, San Benedetto del Tronto, Italy.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/58335 Collegamento a IRIS

2010
Caratterizzazione chimico-fisica di una nuova classe di vettori sintetici di materiale genetico
Contributi delle microscopie allo sviluppo delle nanotecnologie in campo biomedico: nanodrug delivery
Autore/i: Pisani, Michela; Mobbili, Giovanna; M., Marini; V., Mori; A., Smorlesi; P., Bruni
Classificazione: 4 Contributo in Atti di Convegno (Proceeding)
Abstract: Contributi delle microscopie allo sviluppo delle nanotecnologie in campo biomedico: nanodrug delivery, Roma 2010
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/83395 Collegamento a IRIS

2010
Preparation and characterization of neutral liposomal delivery systems for DNA transfections
2nd Conference Innovation in drug delivery: from preformulation to development trough innovative evaluation process.
Autore/i: Pisani, Michela; Mobbili, Giovanna; M., Marini; V., Mori; A., Smorlesi; P., Bruni
Classificazione: 4 Contributo in Atti di Convegno (Proceeding)
Abstract: 2nd Conference Innovation in Drug Delivery: From Preformulation to Development through innovative Evaluation Process, Aix-en-Provence, France
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/83396 Collegamento a IRIS

2010
Sintesi e caratterizzazione strutturale di vettori sintetici di DNA sensibili al pH
XIX Congresso interregionale TUMA
Autore/i: Mobbili, Giovanna; P., Bruni; M., Marini; V., Mori; Pisani, Michela
Classificazione: 5 Altro
Abstract: XIX Congresso interregionale TUMA 2010, Ancona, Italy.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/58337 Collegamento a IRIS

2009
Sintesi ed applicazione di sistemi lipidici come vettori in terapia genica
XXVIII Congresso TUMA
Autore/i: Bruni, P.; Marini, M.; Mobbili, Giovanna; Pisani, Michela
Classificazione: 4 Contributo in Atti di Convegno (Proceeding)
Abstract: XXVIII Congresso interregionale TUMA 2009 Tirrenia (Pisa) Italy
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/83393 Collegamento a IRIS

2009
I liposomi neutri nel trasferimento genico: da helpers dei cationici a protagonisti
XXVIII Congresso TUMA
Autore/i: Bruni, P.; Marini, M.; Mobbili, Giovanna; Pisani, Michela
Classificazione: 4 Contributo in Atti di Convegno (Proceeding)
Abstract: XXVIII Congresso interregionale TUMA 2009 Tirrenia (Pisa) Italy
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/83392 Collegamento a IRIS

2009
Nanostructure and Biological Function of Self-Assembled Liposome-DNA-Metal Complexes for Gene Therapy
Proceedings of the International School on Advanced Material Science and Technology, Nanobiotechnologies and Nanomedicine
Autore/i: Francescangeli, Oriano; Pisani, Michela; V., Fino; Bruni, Paolo
Editore: Edizioni GEI - Fondazione Cassa di Risparmio di Jesi
Luogo di pubblicazione: JESI
Classificazione: 4 Contributo in Atti di Convegno (Proceeding)
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/53460 Collegamento a IRIS

2009
12-Crown-4-based amphipatic lipid and corresponding metal cation complexes for gene therapy applications: FT-IR characterization and surface charge determination
JOURNAL OF MOLECULAR STRUCTURE
Autore/i: Bruni, P; Fino, V; Pisani, Michela; Tosi, Giorgio; Stipa, Pierluigi; Ferraris, P; Francescangeli, Oriano
Classificazione: 1 Contributo su Rivista
Abstract: The new lipid 1,2-O-dioleyl-3-O-{2-[(12-crown-4)-methoxy]-ethyl}-sn-glycerol, 12C4L, has been synthesized. This molecule can coordinate different cations that should make the corresponding liposome a good candidate as vector of genetic material for possible applications in gene therapy. An important feature of the molecule is the possibility to modulate the net surface charge of their complexes with metal cations, which is important to provide efficient DNA transfections. The molecule and its complexes with some metal cations (Mg2+, Ca2+, Mn2+) have been characterized by FT-IR spectroscopy and band attributions confirmed by Density Functional Theory calculations. The net surface charge has been determined by Z potential determinations.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/50926 Collegamento a IRIS

2009
DNA Condensation into Inverted Hexagonal Phase in Aqueous Dispersion of Poly(Ethylene)-Functionalized Dioleoylphosphatidylethanolamine and Metal Cations
MOLECULAR CRYSTALS AND LIQUID CRYSTALS
Autore/i: Pisani, Michela; Fino, V.; Bruni, Paolo; Francescangeli, Oriano
Editore: TAYLOR & FRANCIS LTD, 4 PARK SQUARE, MILTON PARK, ABINGDON, ENGLAND, OXON, OX14 4RN
Classificazione: 1 Contributo su Rivista
Abstract: We report the x-ray diffraction study of the structure and phase behavior of mixtures of dioleoylphosphatidylethanolamine (DOPE) and poly(ethyleneglycol)- functionalized DOPE (DOPE:PEG(350)) in aqueous dispersions of DNA and bivalent metal cations. Characterization of the phase behaviour of DOPE/DOPE:PEG(350) mixtures in presence of metal cations without DNA is also performed: over a definite amount of the PEG-lipid component, a phase transition from the inverted hexagonal phase HII to the bicontinuous inverted cubic phase, with space group Pn3m is induced. Moreover, it is shown that at low concentration of PEG-lipid component, the inverted hexagonal phase [image omitted] is found on adding DNA to the aqueous dispersions of DOPE/DOPE:PEG and metal cations. This structure consists of cylindrical DNA strands coated by neutral lipid monolayers and arranged on a two-dimensional hexagonal lattice. The results here shown represent the first experimental evidence of a self-assembled formation of an inverted hexagonal complex structure in aqueous dispersions of DNA, metal cations and liposomes made of mixtures of pure and pegylated lipids. The surface functionalization with the PEG-lipid and the evidence of the formation of the complexes are important results in the perspective of the development of appropriate vectors for drug delivery and gene transfection.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/50160 Collegamento a IRIS

2008
Structure and supramolecular organization of a new class of self-assembled Lipid-DNA-Metal Complexes for gene transfer
8° Congresso Nazionale della Società Italiana dei Cristalli Liquidi
Autore/i: Pisani, Michela; V., Fino; P., Bruni; Francescangeli, Oriano
Classificazione: 4 Contributo in Atti di Convegno (Proceeding)
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/83378 Collegamento a IRIS

2008
Metal Cation Induced Cubic Phase in Poly(ethylene glycol)-Functionalized Dioleoylphosphatidylethanolamine Aqueous Dispersions
JOURNAL OF PHYSICAL CHEMISTRY. B, CONDENSED MATTER, MATERIALS, SURFACES, INTERFACES & BIOPHYSICAL
Autore/i: Pisani, Michela; V., Fino; P., Bruni; E., DI COLA; Francescangeli, Oriano
Classificazione: 1 Contributo su Rivista
Abstract: Metal cations (Mn2+ or Ca2+) in aqueous dispersions of mixtures of dioleoylphosphatidylethanolamine (DOPE) and poly(ethylene glycol)-functionalized DOPE (DOPE-PEG(350)) induce, above a certain amount of the PEG lipid component, a phase transition from the inverted hexagonal phase HII to the bicontinuous inverted cubic phase Q224 with space group Pn3m. The process is driven by the decrease of free elastic energy due to the Gaussian curvature of the cubic phase. The structural characterization of the phase behavior over the whole explored range of DOPE-PEG/DOPE weight ratio (3−25%) is reported, focusing on the role of the metal cation in the formation of the 3D cubic lattice. This result may represent a significant progress toward a design-based approach to drug delivery.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/53086 Collegamento a IRIS

2008
“Nanoaggregates of Copper Porphyrazine in Floating Layers and Langmuir-Schaefer films”
LANGMUIR
Autore/i: L. A., Valkova; L., Valli; S., Casilli; G., Giancane; N. Y., Borovkov; G. V., Sibrina; A. S., Glibin; O. I., Koifman; Pisani, Michela; Rustichelli, Franco
Editore: American Chemical Society:1155 Sixteenth Street Northwest:Washington, DC 20036:(800)227-5558, EMAIL: service@acs.org, INTERNET: http://www.pubs.acs.org, Fax: (614)447-3671
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/36950 Collegamento a IRIS

2007
Structure and function of self-assembled liposome-DNA-metal complexes for gene transfer
European Synchrotron Radiation Facility Highlights 2006
Autore/i: P., Bruni; Pisani, Michela; A., Amici; C., Marchini; M., Montani; Francescangeli, Oriano
Editore: G. Adams
Classificazione: 2 Contributo in Volume
Abstract: Invited Contribution
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/42226 Collegamento a IRIS

2007
Vettori Sintetici di DNA per impieghi in terapia genica: sintesi, determinazione strutturale, trasfezione cellulare
Atti del XXXI Convegno Nazionale dela Divisione di Chimica Organica della Società Chimica Italiana
Autore/i: Pisani, Michela; V., Fino; Francescangeli, Oriano; Bruni, Paolo
Editore: Università della Calabria
Luogo di pubblicazione: ARCAVACATA DI RENDE
Classificazione: 4 Contributo in Atti di Convegno (Proceeding)
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/83372 Collegamento a IRIS

2007
Liposomi-DNA:Nuovi sistemi per il trasporto di materiale genetico
XXXI Convegno Nazionale della Divisione di Chimica Organica della Società Chimica Italiana
Autore/i: V., Fino; P., Bruni; Francescangeli, Oriano; Pisani, Michela
Editore: Università della Calabria
Luogo di pubblicazione: ARCAVACATA DI RENDE
Classificazione: 4 Contributo in Atti di Convegno (Proceeding)
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/83375 Collegamento a IRIS

2006
Structure and function of self-assembled liposome-DNA-metal complexes for gene transfer
Proceedings of the Fifth International Conference on Synchrotron Radiation in Materials Science SRMS-5,
Autore/i: Francescangeli, Oriano; Pisani, Michela; Bruni, Paolo; C., Marchini; M., Montani; A., Amici
Editore: Argonne National Laboratory
Luogo di pubblicazione: CHICAGO
Classificazione: 4 Contributo in Atti di Convegno (Proceeding)
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/51584 Collegamento a IRIS

2006
Self-assembled ternary complexes of neutral liposomes, DNA and bivalent metal cations for gene delivery
V Convegno Nazionale AICIng
Autore/i: Pisani, Michela; Francescangeli, Oriano; P., Bruni
Classificazione: 4 Contributo in Atti di Convegno (Proceeding)
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/83369 Collegamento a IRIS

2006
Pressure effects on lipidic direct phases: the dodecyltrimethyl ammonium chloride-water system
JOURNAL OF PHYSICAL CHEMISTRY. B, CONDENSED MATTER, MATERIALS, SURFACES, INTERFACES & BIOPHYSICAL
Autore/i: L., Paccamiccio; Pisani, Michela; Spinozzi, Francesco; C., Ferrero; S., Finet; Mariani, Paolo
Classificazione: 1 Contributo su Rivista
Abstract: The direct lyotropic polymorphism of dodecyltrimethylammonium chloride (DTAC) was investigated by synchrotron X-ray diffraction at different water concentrations under compression up to 2 kbar, i.e., in the pressure intermediate range where interesting biophysical transformations occur and the functional characteristics of cell membranes are altered. The results show that pressure induces the transition from the hexagonal phase to the micellar Pm3n cubic phase in hydrated samples (c between 0.5 and 0.6, c being the weight concentration of lipid in the mixture) and the transition from the bicontinuous Ia3d cubic phase to the hexagonal phase in drier samples (c = 0.8). By increasing the pressure on very dry samples, a lamellar Lα phase was observed to form transitorily at the Ia3d cubic-hexagonal phase transition. Phase compressibility and then the lipid and water partial molecular compressibilities were derived as a function of pressure and concentration. As a result, we assessed the very low compressibility of the hydration water within the lipid phases, and we demonstrated that the compressibility of DTAC is very dependent on pressure. Moreover, the molecular parameters of DTAC calculated in the different phases during compression confirmed that pressure induces small but continuous conformational changes, definitely different from the large changes observed in lipid molecules forming type II structures. © 2006 American Chemical Society.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/53248 Collegamento a IRIS

2006
Self-assembled ternary complexes of neutral liposomes, deoxyribonucleic acid, and bivalent metal cations. Promising vectors for gene transfer?
APPLIED PHYSICS LETTERS
Autore/i: Bruni, Paolo; Pisani, Michela; A., Amici; C., Marchini; M., Montani; Francescangeli, Oriano
Classificazione: 1 Contributo su Rivista
Abstract: By means of synchrotron x-ray diffraction we demonstrate the self-assembled formation of the neutral ternary dioleoyl-phosphatidylcholine-deoxyribonucleic acid (plasmid)−Me2+ (Me = Ca and Mn) complexes in the liquid-crystalline Lα phase. We also report an attempt of an in vitro transfection on mouse fibroplast NIH 3T3 cell lines, which shows the capability of these complexes to transfect DNA. Based on the reported results, efficient encapsulation of DNA plasmids in these ternary neutral complexes may represent an important alternative to current systemic gene approaches.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/57642 Collegamento a IRIS

2006
Structure and Phase Behavior of Self-Assembled DPPC-DNA-Metal Cation Complexes
THE JOURNAL OF PHYSICAL CHEMISTRY. B
Autore/i: Pisani, Michela; Bruni, Paolo; G., Caracciolo; R., Caminiti; Francescangeli, Oriano
Editore: Columbus, OH : American Chemical Society, 1997-
Classificazione: 1 Contributo su Rivista
Abstract: Multilamellar liposomes of dipalmitoylphosphatidylcholine (DPPC) in solution with DNA and bivalent metal cations (Ca2+, Mn2+, Mg2+) self-assemble into a ternary DPPC−DNA−Me2+ complex. The supramolecular structure of the complex consists of an ordered multilamellar assembly where hydrated DNA helices are sandwiched between the lipid bilayers and the metal cations bind the phosphate groups of DNA to the lipid polar heads. In the range of explored incubation times, the complex coexists with the uncomplexed DPPC over the whole temperature range investigated (20−55 °C). Accordingly, two distinct coexisting lamellar phases are observed, one corresponding to the ternary complex and the other to the uncomplexed lipid. The structure and thermotropic phase behavior of both of these have been investigated by means of synchrotron X-ray diffraction, and the relevant structural data are deduced from experimental electron density profiles. While the uncomplexed lipid exhibits the same phase behavior as pure DPPC, that is, Lβ‘−Pβ‘−Lα, the thermotropic behavior of the bound lipid in the complex is partially altered. This is manifested as an increase in the main transition temperature and the disappearance of the ripple phase leading to the single − phase transition. The role of the different metal cations in promoting and stabilizing the DNA condensation into the ternary complex is also discussed.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/53216 Collegamento a IRIS

2005
“Effect of hydration on the structure of oriented lipid membranes investigated by in situ time-resolved energy dispersive x-ray diffraction”
APPLIED PHYSICS LETTERS
Autore/i: R., Caminiti; G., Caracciolo; Pisani, Michela
Editore: American Institute of Physics:2 Huntington Quadrangle, Suite 1NO1:Melville, NY 11747:(800)344-6902, (631)576-2287, EMAIL: subs@aip.org, INTERNET: http://www.aip.org, Fax: (516)349-9704
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/36899 Collegamento a IRIS

2005
Supramolecular Ordering of Self-Assembled Lipid-DNA-Metal Complexes related to gene therapy
8th European Conference on Liquid Crystals
Autore/i: Francescangeli, Oriano; Pisani, Michela; P., Bruni
Classificazione: 4 Contributo in Atti di Convegno (Proceeding)
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/83356 Collegamento a IRIS

2005
Studi Strutturali di complessi ternari Lipidi-DNA-ioni metallici
XXIV Congresso TUMA
Autore/i: Pisani, Michela; Francescangeli, Oriano; P., Bruni
Classificazione: 4 Contributo in Atti di Convegno (Proceeding)
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/83359 Collegamento a IRIS

2005
Self-assembled liposome-DNA-metal complexes related to DNA delivery
MOLECULAR CRYSTALS AND LIQUID CRYSTALS
Autore/i: Pisani, Michela; Bruni, Paolo; Conti, C.; Giorgini, Elisabetta; Francescangeli, Oriano
Classificazione: 1 Contributo su Rivista
Abstract: We have carried out a systematic structural study of novel liquid-crystal phases of self-assembled neutral lipid-DNA-metal cation complexes in water solution. Small-angle X-ray scattering experiments have given new insight into the structures of these ternary complexes. We have shown that different divalent metal cations are equally active in promoting the DNA condensation into the ternary complex when different lipid and DNA species are used. In addition, strong indication has been found that the phase of the complexes reflects the structure and symmetry of the parent lipid phase. These achievements provide an important structure-composition correlation that may drive the best design of these materials for future applications as non-viral DNA carriers in gene therapy.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/53121 Collegamento a IRIS

2005
Fe2+ promoted peroxidation of 1,2-diacyl-sn-glycero-3-phosphocholine liposomes in the presence of calf thymus DNA.
ORGANIC & BIOMOLECULAR CHEMISTRY
Autore/i: Bruni, Paolo; Pisani, Michela; Iacussi, M; Francescangeli, Oriano
Classificazione: 1 Contributo su Rivista
Abstract: The peroxidation reaction of some liposomes, namely egg yolk phosphatidylcholine (PC), dioleoyl- (DOPC) and dilinoleoyl- (DLPC) phosphocholines, promoted by ferrous ions (Fenton reaction) has been studied at the physiological pH value, in the absence and in the presence of calf thymus DNA. A catalytic effect of DNA, where the lag time reduces or is completely annihilated, together with an increase in both the yields and the rates of the reactions, has been observed. This effect of DNA has been attributed to the ability of the three components, liposomes, DNA and Fe2+, to form a stable ternary complex, which produces a reduction of the undulatory fluctuations of the hydrocarbon tails of liposomes and strengthens the packing between the acyl chains in the lipid bilayers, with the consequence of enhancing the liposome crystallinity.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/52650 Collegamento a IRIS

2005
Effect of hydration on the long-range order of lipid multilayers investigated by in situ time-resolved energy dispersive X-ray diffraction
CHEMICAL PHYSICS LETTERS
Autore/i: Caminiti, R; Caracciolo, G; Pisani, Michela; Bruni, Paolo
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/52956 Collegamento a IRIS

2004
“Liposome-DNA-metal complxes related to DNA delivery”.
20th International Liquid Crystal Conference
Autore/i: Pisani, Michela; Francescangeli, Oriano; Bruni, P.; Giorgini, Elisabetta; Conti, C.
Classificazione: 4 Contributo in Atti di Convegno (Proceeding)
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/83352 Collegamento a IRIS

2004
Supramolecular Ordering of Self-Assembled Lipid-DNA-Metal Complexes probed by X Ray diffraction
6th National Meeting SICL 2004
Autore/i: Francescangeli, Oriano; Pisani, Michela; P., Bruni
Classificazione: 4 Contributo in Atti di Convegno (Proceeding)
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/83354 Collegamento a IRIS

2004
Structure of solid-supported lipid-DNA-metal complexes investigated by energy dispersive X-ray diffraction
CHEMICAL PHYSICS LETTERS
Autore/i: Caracciolo, G; Sadum, C; Caminiti, R; Pisani, Michela; Bruni, Paolo; Francescangeli, Oriano
Classificazione: 1 Contributo su Rivista
Abstract: When mixing aqueous solutions of DNA and dioloeyl-phosphatidylcholine multilamellar liposomes in the presence of Mn 2+ ions, a multilamellar liquid-crystalline phase is formed in which DNA monolayers are comprised between opposing lipid bilayers. Here, we report on a new experimental procedure resulting in the formation of solid-supported DOPC-DNA-Mn 2+ complexes in the biologically relevant excess water condition. The supramolecular structure of solid-supported complexes, characterized by means of Energy Dispersive X-ray Diffraction, exhibits very similar structural properties with respect to the solution structure of the complexes as revealed by synchrotron Small Angle X-ray Scattering measurements.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/51766 Collegamento a IRIS

2004
Histological and microscopy FT-IR imaging study on the proliferative activity and angiogenesis in head and neck tumours
FARADAY DISCUSSIONS
Autore/i: Bruni, P; Conti, Carla; Giorgini, Elisabetta; Pisani, Michela; Rubini, Corrado; Tosi, Giorgio
Classificazione: 1 Contributo su Rivista
Abstract: Micro Fourier transform infrared spectroscopy enables one to study small samples because of the high quality spectra that can be obtained. Biochemical and morphological changes between control and pathological tissues of head and neck tumours have been monitored drawing three-dimensional chemical maps of the main vibrational modes in the regions of interest. Comparison between spectral and histological data shows a satisfactory degree of accordance. Among all, proliferating and regressive states of the tumours can be identified.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/53277 Collegamento a IRIS

2004
Pressure effects on columnar lyotropics: anisotropic compressibilities in guanosine mono-phosphate four-stranded helices
JOURNAL OF PHYSICAL CHEMISTRY. B, CONDENSED MATTER, MATERIALS, SURFACES, INTERFACES & BIOPHYSICAL
Autore/i: Ausili, P.; Pisani, Michela; Finet, S.; Amenitsch, H.; Ferrero, C.; Mariani, Paolo
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/53157 Collegamento a IRIS

2004
“Evidence of an inverted hexagonal phase in self-assembled phospholipid-DNA-metal complexes”
EUROPHYSICS LETTERS
Autore/i: Francescangeli, Oriano; Pisani, Michela; V., Stani; Bruni, Paolo; T. M., Weiss
Editore: EDP Sciences:17 Ave Du Hoggar, BP112, 91944 Les Ulis Cedex A France:011 33 1 69187575, EMAIL: subscribers@edpsciences.com, INTERNET: http://www.edpsciences.com, Fax: 011 33 1 69860678
Classificazione: 1 Contributo su Rivista
Abstract: We report the first observation of an inverted hexagonal phase of phospholipid- DNA-metal complexes. These ternary complexes are formed in a self-assembled manner when water solutions of neutral lipid dioleoylphosphatidylethanolamine (DOPE), DNA and divalent metal cations (Me2+; Me = Fe, Co, Mg, Mn) are mixed, which represents a striking example of supramolecular chemistry. The structure, derived from synchrotron X-ray diffraction, consists of cylindrical DNA strands coated by neutral lipid monolayers and arranged on a two-dimensional hexagonal lattice (Hc II ). Besides the fundamental aspects, DOPE-DNA-Me2+ complexes may be of great interest as efficient nonviral delivery systems in gene therapy applications because of the low inherent cytotoxicity and the potential high transfection efficiency.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/36700 Collegamento a IRIS

2003
“Pressure effects on columnar lyotropics: X-ray diffraction investigation on d(GMP) and GMP”
Advances in High Pressure Bioscience and Biotechnology II
Autore/i: P., Ausili; Pisani, Michela; C., Ferrero; S., Finet; Mariani, Paolo
Editore: R. Winter
Classificazione: 2 Contributo in Volume
Abstract: Springer-Verlag (Heidelberg)
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/42232 Collegamento a IRIS

2003
Supramolecular ordering of self-assembled liposome-DNA-metal complexes
Recent Research Developments in Macromolecules
Autore/i: Francescangeli, Oriano; Pisani, Michela; V., Stanic; Bruni, Paolo; M., Iacussi
Editore: Research Signpost
Luogo di pubblicazione: TRIVANDRUM-695 023, KERALA
Classificazione: 2 Contributo in Volume
Abstract: Invited article
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/51663 Collegamento a IRIS

2003
“Pressure and Temperature effects on phase behaviour and structural properties of lipids mixture”
Congresso Nazionale di Fisica della Materia
Autore/i: L., Paccamiccio; Pisani, Michela; G. M., Di Gregorio; Mariani, Paolo
Classificazione: 4 Contributo in Atti di Convegno (Proceeding)
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/83351 Collegamento a IRIS

2003
“Supramolecular structure of self-assembled liposome-DNA-metal complexes”
Congresso Nazionale di Fisica della Materia
Autore/i: Pisani, Michela; V., Stanic; P., Bruni; M., Iacussi; G., Tosi; Francescangeli, Oriano
Classificazione: 4 Contributo in Atti di Convegno (Proceeding)
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/83339 Collegamento a IRIS

2003
“Liquid-crystal ordering in confined geometries probed by X-ray microdiffraction"
Congresso Nazionale di Fisica della Materia
Autore/i: Francescangeli, Oriano; C., Ferrero; V., Stanic; Pisani, Michela; Lucchetti, Liana; M., Burghammer
Classificazione: 4 Contributo in Atti di Convegno (Proceeding)
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/83347 Collegamento a IRIS

2003
Compressing inverse lyotropic systems: structural behavior and energetics of dioleoyl phosphatidyl ethanolamine
PHYSICAL REVIEW E, STATISTICAL, NONLINEAR, AND SOFT MATTER PHYSICS
Autore/i: Pisani, Michela; Narayanan, T.; DI GREGORIO, G.; Ferrero, C.; Finet, S.; Mariani, Paolo
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/50169 Collegamento a IRIS

2003
Small-angle X-ray scattering and neutron reflectivity studies of Langmuir-Blodgett films of copper tetra-tert-butyl-azaporphyrines
JOURNAL OF APPLIED CRYSTALLOGRAPHY
Autore/i: L. A., Valkova; A., Menelle; N., Borovkov; V., Erokhin; Pisani, Michela; F., Ciuchi; Carsughi, Flavio; Spinozzi, Francesco; M., Pergolini; R., Padke; S., Bernstorff; Rustichelli, Franco
Classificazione: 1 Contributo su Rivista
Abstract: Langmuir-Blodgett (LB) films of tetra-tert-butyl-substituted copper azaporphyrines, namely phthalocyanine (CuPc′), tetra-3-nitrophthalocyanine (CuPc*) and porphyrazine (CuPaz′), are studied by small-angle scattering technique. Two kinds of systems are under investigation: first, the CuPc′/Cd-stearate-d35 superlattices; second, the LB films of CuPc′, CuPc* and CuPaz′ doped with benzene-d6. X-ray diffraction patterns of the superlattices exhibit bilayer structural units indicating existence of not only 1D but also 3D nano-aggegates of CuPc′. The neutron reflectivity study on the films heavily doped with benzene-d6 indicates that π-deficiency and size of the azaporphyrine nuclei favor interactions between the nano-aggregates.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/51312 Collegamento a IRIS

2002
“Compressing inverse lyotropic phases: effects on dioleoyl phosphatidyl ethanolamine at low hydration”
Congresso Nazionale di Fisica della Materia
Autore/i: Pisani, Michela; T., Narayanan; G. M., Di Gregorio; C., Ferrero; L., Paccamiccio; Mariani, Paolo
Classificazione: 4 Contributo in Atti di Convegno (Proceeding)
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/83332 Collegamento a IRIS

2002
Structure of Monolayers of Copper Tetra-(3-nitro-5-tert-butyl)-Phthalocyanine at the Air-water Interface.
LANGMUIR
Autore/i: Valkova, L. A.; N., Borovkov; Pisani, Michela; Rustichelli, Franco
Classificazione: 1 Contributo su Rivista
Abstract: The structure of floating layers of soluble copper tetra-(3-nitro-5-tert-butyl)-phthalocyanine (CuPc*) is studied through surface pressure-area (π-A) isotherms measured at various initial surface concentrations (N0) of CuPc*. In the gaseous layers, the CuPc* molecules are surrounded by a hydration sphere. Condensation of the layers under compression results in an increase in the tilt angle of the CuPc* molecules relative to the air-water interface. Though it is accompanied by a loss of weakly bound water, an inner part of the hydration sphere sustains compression and enters the condensed layers. Depending on the N0 and π values, the molecular tilt angle ranges from 30 to 90° indicating the phase states of the β-, α-, and x-types. The different nature of the bilayers formed through compression of the rare monolayers and through spreading of large amounts of CuPc* is stated.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/53317 Collegamento a IRIS

2002
Compressing inverse lyotropic phases: effects on dioleoyl phosphatidylethanolamine at low hydration
Network SINBIO- Luce di Sincrotrone e sistemi biologici
Autore/i: Pisani, Michela
Classificazione: 4 Contributo in Atti di Convegno (Proceeding)
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/83330 Collegamento a IRIS

2002
"Some features of the molecular assembly of copper porphyrazines.”
MATERIALS SCIENCE AND ENGINEERING. C, BIOMIMETIC MATERIALS, SENSORS AND SYSTEMS
Autore/i: L. A., Valkova; N., Borovkov; V., Kopranenkov; Pisani, Michela; Bossi, M.; F., Rustichelli
Editore: Elsevier BV:PO Box 211, 1000 AE Amsterdam Netherlands:011 31 20 4853757, 011 31 20 4853642, 011 31 20 4853641, EMAIL: nlinfo-f@elsevier.nl, INTERNET: http://www.elsevier.nl, Fax: 011 31 20 4853598
Classificazione: 1 Contributo su Rivista
Abstract: Floating layers and Langmuir-Blodgett (LB) films of copper porphyrazine (CuPaz) and its tetra-tert-butyl-substituted homologue (CuPaz') are studied. Contrary to phthalocyanines, the monolayer phase in the porphyrazine layers is metastable and transforms directly into the tetralayer one under moderate compression. In diffraction patterns and electronic spectra of the LB films, supramolecular peaks indicating collectivizing of the molecular electron density in direction perpendicular to the main axis of the macrocycle are found. The data obtained indicate the prismatic 3-D supermolecule to be the simplest structural unit of the porphyrazine assembly.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/36696 Collegamento a IRIS

2002
Influence of molecular and supramolecular factors on sensor properties of Langmuir-Blodgett films of tert-buthyl-substituted copper azaporphyrines towards hydrocarbons
COLLOIDS AND SURFACES. A, PHYSICOCHEMICAL AND ENGINEERING ASPECTS
Autore/i: Valkova, L.; Borovkov, N.; Maccioni, E.; Pisani, Michela; Rustichelli, Franco; Erokhin, V.; Patternolli, C.; Nicolini, C.
Classificazione: 1 Contributo su Rivista
Abstract: Langmuir-Blodgett (LB) films of tert-butyl-substituted copper azaporphyrines, namely binuclear phthalocyanine (Cu2Pc2') and porphyrazine (CuPaz'), were formed from the floating layers differently structured. Effect of the film structure on sorption of benzene and hexane by the LB films was studied microgravimetrically. The greatest benzene/hexane selectivity was found for the homogeneous film of Cu2Pc2'.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/53200 Collegamento a IRIS

2001
Azelaic acid and azelaic disodium salt in model membranes. Effects induced on hydration of phospholipid bilayer
Congresso Nazionale di Fisica della Materia
Autore/i: G., Bossi; B., Dubini; M., Pergolini; Pisani, Michela; M. G., Ponzi Bossi; V., Stanic
Classificazione: 4 Contributo in Atti di Convegno (Proceeding)
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/83305 Collegamento a IRIS

2001
Three-dimensional structure of the copper porphyrazine layers at the air-water interface
THIN SOLID FILMS
Autore/i: Valkova, L.; Borovkov, N.; Pisani, Michela; Rustichelli, Franco
Classificazione: 1 Contributo su Rivista
Abstract: The surface pressure-molecular area (pi -A) isotherms for the layers of copper porphyrazine (CuPaz) at the air-water interface are studied at different initial surface concentration (N-0) of CuPaz. A new approach to analysis of the isotherms is proposed. It allows determination of the molecular tilt angle, compressibility factor of the floating layers, the polylayer order, dimensions of the hydration sphere surrounding the spread molecules and work of transition. It is stated that the CuPaz layers are constructed from either two- or three- dimensional supermolecules. The latter are supposed to be a tilted prism with square base of four CuPaz molecules.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/50273 Collegamento a IRIS

2001
“Compressing lyotropic structures: an analysis of direct hexagonal and cubic phases”
Congresso Nazionale di Fisica della Materia, Roma, Palazzo dei Congressi-EUR
Autore/i: Pisani, Michela; C., Ferrero; T., Narayanan; Mariani, Paolo
Classificazione: 4 Contributo in Atti di Convegno (Proceeding)
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/83185 Collegamento a IRIS

2001
Pressure induced cubic-to-cubic phase transition in monoolein hydrated system
JOURNAL OF PHYSICAL CHEMISTRY. B, CONDENSED MATTER, MATERIALS, SURFACES, INTERFACES & BIOPHYSICAL
Autore/i: Pisani, Michela; Bernstorff, S.; Ferrero, C.; Mariani, Paolo
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/50380 Collegamento a IRIS

2000
Description of structural and energetic effects of hydrostatic pressure on lipid cubic phases
Congresso Nazionale di Fisica della Materia
Autore/i: Pisani, Michela; Mariani, Paolo; S., Bernstorff; C., Ferrero
Classificazione: 4 Contributo in Atti di Convegno (Proceeding)
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/83181 Collegamento a IRIS

2000
Temperature dependent structural rearrangements in Azaporphyrines LB films
Congresso Nazionale di Fisica della Materia,
Autore/i: Rustichelli, F.; Valkova, L. A.; Pisani, Michela; Maccioni, E.; Carsughi, F.; Bernstorff, S.
Classificazione: 4 Contributo in Atti di Convegno (Proceeding)
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/83179 Collegamento a IRIS

2000
“Pressure induced cubic-to-cubic phase transition on the monoolein hydrated system”
EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS
Autore/i: Pisani, Michela; Bernstorff, S.; Ferrero, C.; Mariani, P.
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/83182 Collegamento a IRIS

1999
Pressure induced cubic-to-cubic phase transition on the monoolein hydrated system”.
7th International Users‘ Meeting, Sincrotrone
Autore/i: Mariani, Paolo; Pisani, Michela; L., Saturni; S., Bernstorff; C., Ferrero; M., Kriechbaum; M., Steinhart
Classificazione: 4 Contributo in Atti di Convegno (Proceeding)
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/83175 Collegamento a IRIS

1998
Gli effetti della pressione idrostatica sul sistema monoleina-acqua.”
XIV Congresso Nazionale della Societa’ Italiana di Biofisica Pura e Applicata
Autore/i: L., Saturni; Pisani, Michela; Mariani, Paolo; R., Caciuffo
Classificazione: 4 Contributo in Atti di Convegno (Proceeding)
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/83176 Collegamento a IRIS




Università Politecnica delle Marche

P.zza Roma 22, 60121 Ancona
Tel (+39) 071.220.1, Fax (+39) 071.220.2324
P.I. 00382520427