96 pubblicazioni classificate nel seguente modo:

Tumor budding is a prognostic factor in head and neck squamous cell carcinoma: A comprehensive meta-analysis and trial sequential analysis
CRITICAL REVIEWS IN ONCOLOGY HEMATOLOGY
Autore/i: Silva, F. F. V. E.; Caponio, V. C. A.; Perez-Sayans, M.; Padin-Iruegas, M. E.; Mascitti, M.; Chamorro-Petronacci, C. M.; Suarez-Penaranda, J. M.; Lorenzo-Pouso, A. I.
Classificazione: 1 Contributo su Rivista
Abstract: Background: Mortality is linked to tumor budding (TB) in certain neoplasms. TB as a relevant histopathological feature is conditioned by tumor site, a specific study on head and neck squamous cell carcinoma (HNSCC) is needed. Methods: A comprehensive meta-analysis was undertaken to investigate the relationship between TB and HNSCC-related outcomes. Results: Overall, 42 studies were included. Patients harboring high TB reported an Overall Survival (OS) Hazard Ratio (HR) of 2.63 (95% confidential interval (CI) 2.04-3.39; p-value < 0.001), Disease-free Survival (DFS) HR of 1.88 (95%CI 1.57-2.24; p-value <0.001) and Disease-specific Survival (DSS) HR of 2.14 (95%CI 1.81-2.52; p-value <0.001). Lymph Node Metastasis (LNM) studies harbored null heterogeneity and marked association with TB (Odds Ratio (OR) = 4.48, 95%CI 2.97-6.76; p-value < 0.001). Trial Sequential Analysis (TSA) supported definitive results for DSS. Conclusion: The study has provided compelling evidence that there is a significant association between TB and a worse prognosis for HNSCC.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/327395

High-risk TP53 mutations predict poor primary treatment response of patients with head and neck squamous cell carcinoma
ORAL DISEASES
Autore/i: Caponio, V. C. A.; Zhurakivska, K.; Mascitti, M.; Togni, L.; Spirito, F.; Cirillo, N.; Lomuzio, L.; Troiano, G.
Classificazione: 1 Contributo su Rivista
Abstract: Objectives: Head and neck squamous cell carcinoma (HNSCC) poses a diagnostic and therapeutic challenge worldwide and is associated with a poor survival rate. Due to the variability in the efficacy of treatments for HNSCC, new predictive biomarkers of therapy outcomes are needed. Recently, we developed an algorithm that employs the mutational profile of TP53 as an independent prognostic factor in HNSCC. In this study, we investigated its role as a predictive biomarker of treatment outcomes in HNSCC patients. We also tested the usefulness of two classification systems for TP53 mutational landscapes. Materials and Methods: Clinical and genomic data were retrieved from The Cancer Genome Atlas database. We built a multivariate stepwise backward binary regression model to assess the role of TP53 mutations in predicting therapeutic outcomes. Results: Cases harbouring high-risk-of-death mutations reported an odds ratio of 3.301 for stable or progressive disease compared to wild-type cases, while no significant difference in treatment outcomes was found between cases with low-risk-of-death mutations and wild-type TP53. Our analysis found that older patients with a history of alcohol consumption had a higher risk of stable/progressive disease. Conclusions: This study improves current evidence on the role of TP53 mutations in treatment response in HNSCC patients.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/327392

The Emerging Role of Salivary Oxidative Stress Biomarkers as Prognostic Markers of Periodontitis: New Insights for a Personalized Approach in Dentistry
JOURNAL OF PERSONALIZED MEDICINE
Autore/i: Viglianisi, G.; Tartaglia, G. M.; Santonocito, S.; Amato, M.; Polizzi, A.; Mascitti, M.; Isola, G.
Classificazione: 1 Contributo su Rivista
Abstract: Periodontitis is a multifactorial and infective oral disease that leads to the destruction of periodontal tissues and tooth loss. Although the treatment of periodontitis has improved recently, the effective treatment of periodontitis and the periodontitis-affected periodontal tissues is still a challenge. Therefore, exploring new therapeutic strategies for a personalized approach is urgent. For this reason, the aim of this study is to summarize recent advances and the potential of oxidative stress biomarkers in the early diagnosis and personalized therapeutic approaches in periodontitis. Recently, ROS metabolisms (ROMs) have been studied in the physiopathology of periodontitis. Different studies show that ROS plays a crucial role in periodontitis. In this regard, the reactive oxygen metabolites (ROMs) started to be searched for the measures of the oxidizing capacity of the plasma understood as the total content of oxygen free radicals (ROS). The oxidizing capacity of plasma is a significant indicator of the body’s oxidant state as well as homocysteine (Hcy), sulfur amino acid, which has pro-oxidant effects as it favors the production of superoxide anion. More specifically, the thioredoxin (TRX) and peroxiredoxin (PRX) systems control reactive oxygen species (ROS), such as superoxide and hydroxyl species, to transduce redox signals and change the activities of antioxidant enzymes to remove free radicals. Superoxide dismutase (SOD), catalase, and glutathione peroxidase (GPx), among other antioxidant enzymes, change their activity when ROS are produced in order to neutralize free radicals. The TRX system is triggered and transduces redox signals to do this.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/315054

Cost/effectiveness analysis of treatment options for the rehabilitation of the total edentulous mandible
JOURNAL OF ORAL REHABILITATION
Autore/i: Zhurakivska, Khrystyna; Luciano, Rossella; Carlo Alberto Caponio, Vito; Lo Russo, Lucio; Lo Muzio, Lorenzo; Mascitti, Marco; Troiano, Giuseppe
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/315053

LGALS3BP is a potential target of antibody-drug conjugates in oral squamous cell carcinoma
ORAL DISEASES
Autore/i: Cela, I.; Caponio, V. C. A.; Capone, E.; Pinti, M.; Mascitti, M.; Togni, L.; Lomuzio, L.; Rubini, C.; Delaurenzi, V.; Lattanzio, R.; Perrotti, V.; Sala, G.
Classificazione: 1 Contributo su Rivista
Abstract: Objective: The aim of the present study was to evaluate the expression of intracellular and vesicular LGALS3BP in oral squamous cell carcinoma (OSCC) patients and available cell lines to explore its potential as a target for antibody-drug conjugate (ADC) therapy. Methods: Free and vesicular LGALS3BP expression levels were evaluated in cancer tissues from a cohort of OSCC patients as well as in a panel of OSCC cell lines through immunohistochemistry, qRT-PCR, Western Blot analysis, and ELISA. Results: LGALS3BP resulted in being highly expressed in the cytoplasm of tumour cells in OSCC patient tissues. A strong correlation was found between high LGALS3BP expression levels and aggressive histological features of OSCC. Biochemistry analysis performed on OSCC cell lines showed that LGALS3BP is expressed in all the tested cell lines and highly enriched in cancer-derived extracellular vesicles. Moreover, LGALS3BP high-expressing HOC621 and CAL27 OSCC cell lines showed high sensitivity to the ADC-payload DM4, with an IC50 around 0.3 nM. Conclusions: The present study highlights that LGALS3BP is highly expressed in OSCC suggesting a role as a potential diagnostic biomarker and therapeutic target for ADC-based therapy.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/327393

Impact of periodontitis on circulating cell-free DNA levels as a measure of cardiovascular disease
CLINICAL ORAL INVESTIGATIONS
Autore/i: Isola, G.; Polizzi, A.; Mascitti, M.; Santonocito, S.; Ronsivalle, V.; Cicciu, M.; Pesce, P.
Classificazione: 1 Contributo su Rivista
Abstract: Objectives: The present study aims to assess the serum circulating cell-free (cfDNA) concentrations in patients with periodontitis and cardiovascular disease (CVD) and to evaluate the impact of periodontitis on circulating cfDNA levels and the confounding factors that might mediated the possible relationship. Materials and methods: Healthy controls (n=30) and patients with CVD (n=31), periodontitis (n=31), and periodontitis + CVD (n=30) were enrolled in the present study. All subjects underwent regular periodontal examination and blood sampling and cfDNA evaluation. The analysis of the plasma cfDNA concentrations was performed using a dsDNA Assay Kit. Results: In comparison with healthy controls and CVD patients, periodontitis and periodontitis+CVD exhibited significantly higher expression of circulating cfDNA (p<0.05). There was a positive correlation among plasma cfDNA and clinical attachment loss (CAL) (p=0.019), high sensitivity C-reactive protein (hs-CRP) (p=0.027), and periodontal inflamed surface area (PISA) (p=0.003). Furthermore, the multivariate regression analysis evidenced that PISA (p<0.001), hs-CRP (p=0.014), and full-mouth bleeding score (FMBS) (p=0.004) were significant predictors of circulating cfDNA concentrations. Conclusions: The results of the study highlighted that the periodontitis and periodontitis + CVD group showed higher circulating cfDNA expression in comparison with healthy controls and CVD patients. Moreover, the extent of periodontitis was correlated with the increased cfDNA levels and represented a significant predictor of the increased circulating cfDNA concentrations. Clinical relevance: Unbalanced circulating cfDNA concentrations have been indicated to represent a possible risk of CVD and endothelial dysfunction. Periodontitis and periodontitis + CVD patients showed higher circulating cfDNA expression; moreover, the extent of periodontitis significantly predicted higher circulating cfDNA concentrations, suggesting the potential increased risk of developing CVD in periodontitis patients.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/327394

A Reciprocal Link between Oral, Gut Microbiota during Periodontitis: The Potential Role of Probiotics in Reducing Dysbiosis-Induced Inflammation
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Autore/i: Di Stefano, M.; Santonocito, S.; Polizzi, A.; Mauceri, R.; Troiano, G.; Lo Giudice, A.; Romano, A.; Mascitti, M.; Isola, G.
Classificazione: 1 Contributo su Rivista
Abstract: Human body is colonized by a florid microbial community of bacteria, archaea, fungi, protists, helminths, and viruses, known as microbiota, which co-evolves with the host and influences its health through all stages of its life. It is well known that oral microorganisms form highly structurally and functionally organized multi-species biofilms and establish a network of complex mutual inter-species interactions having a primary function in synergy, signaling, or antagonism. This ecological model allows the microorganisms to increase their resistance to antimicrobial agents and settle a balanced microbes-host symbiotic relationship that ensures oral and global health status in humans. The host-associated microbiome is an important factor in human health and disease. Therefore, to develop novel diagnostic, therapeutic, and preventive strategies, microbiome’s functions and the reciprocal interactions every microbiome entertains with other microbial communities in the human body are being investigated. This review provides an analysis of the literature about the close connection between the two largest microbial communities in humans: the oral and the gut microbiomes. Furthermore, it focuses on how the alteration of their microbial and functional characteristics can lead to and reciprocally influence the onset of both oral and intestinal microbiome-associated illness, along with the potential role of probiotics in ameliorating inflammation and microbial dysbiosis.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/315055

Role Played by Paraoxonase-2 Enzyme in Cell Viability, Proliferation and Sensitivity to Chemotherapy of Oral Squamous Cell Carcinoma Cell Lines
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Autore/i: Campagna, R; Belloni, A; Pozzi, V; Salvucci, A; Notarstefano, V; Togni, L; Mascitti, M; Sartini, D; Giorgini, E; Salvolini, E; Santarelli, A; Lo Muzio, L; Emanuelli, M
Classificazione: 1 Contributo su Rivista
Abstract: Oral squamous cell carcinoma represents the most aggressive and frequent form of head and neck cancer. Due to drug resistance, the 5-year survival rate of patients with advanced disease is less than 50%. In order to identify molecular targets for effective oral cancer treatment, we focused on paraoxonase-2 enzyme. Indeed, based on data previously obtained from preliminary immunohistochemistry and Western blot analyses performed on tissue specimens, the enzyme was found to be upregulated in tumor compared with normal oral mucosa. Therefore, paraoxonase-2 gene silencing was achieved in HSC-3 and HOC621 oral cancer cell lines, and the effect on cell proliferation, viability, apoptosis induction and sensitivity to cisplatin and 5-fluorouracil treatment was evaluated. Fourier Transform InfraRed Microspectroscopy analyzed alterations of cellular macromolecules upon treatment. Enzyme level and cell proliferation were also determined in cisplatin-resistant clones obtained from HOC621 cell line, as well as in parental cells. Reported data showed that paraoxonase-2 knockdown led to a reduction of cell proliferation and viability, as well as to an enhancement of sensitivity to cisplatin, together with the activation of apoptosis pathway. Spectroscopical data demonstrated that, under treatment with cisplatin, oxidative damage exerted on lipids and proteins was markedly more evident in cells down-regulating paraoxonase-2 compared to controls. Interestingly, enzyme expression, as well as cell proliferation were significantly higher in cisplatin-resistant compared with control HOC621 cells. Taken together these results seem to candidate the enzyme as a promising target for molecular treatment of this neoplasm.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/310087

Paraoxonase-2 expression in oral squamous cell carcinoma
HUMAN CELL
Autore/i: Campagna, R; Pozzi, V; Salvucci, A; Togni, L; Mascitti, M; Sartini, D; Salvolini, E; Santarelli, A; Lo Muzio, L; Emanuelli, M
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/313648

Prognostic significance of tumor budding thresholds in oral tongue squamous cell carcinoma
ORAL DISEASES
Autore/i: Mascitti, Marco; Togni, Lucrezia; Caponio, Vito Carlo Alberto; Zhurakivska, Khrystyna; Lo Muzio, Lorenzo; Rubini, Corrado; Santarelli, Andrea; Troiano, Giuseppe
Classificazione: 1 Contributo su Rivista
Abstract: Oral tongue squamous cell carcinoma (OTSCC) represents the most common malignancy of the oral cavity. Tumor budding (TB) is a reliable prognostic factor in OTSCC; however, a standardized scoring system is not still validated.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/304824

Identification of BRAF V600E mutation in odontogenic tumors by high-performance MALDI-TOF analysis
INTERNATIONAL JOURNAL OF ORAL SCIENCE
Autore/i: Togni, Lucrezia; Zizzi, Antonio; Mazzucchelli, Roberta; Santarelli, Andrea; Rubini, Corrado; Mascitti, Marco
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/302379

Molecules and Biomaterial Technologies Affecting Stem Cell Differentiation
STEM CELLS INTERNATIONAL
Autore/i: Muzio, L. L.; Mascitti, M.; La Noce, M.; Posa, F.; Kudo, Y.; Cirillo, N.
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/315056

The Emerging Impact of Tumor Budding in Oral Squamous Cell Carcinoma: Main Issues and Clinical Relevance of a New Prognostic Marker
CANCERS
Autore/i: Togni, L.; Caponio, V. C. A.; Zerman, N.; Troiano, G.; Zhurakivska, K.; Lo Muzio, L.; Balercia, A.; Mascitti, M.; Santarelli, A.
Classificazione: 1 Contributo su Rivista
Abstract: Tumor Budding (TB) represents a single cancer cell or a small cluster of less than five cancer cells on the infiltrative tumor front. Accumulating evidence suggests TB is an independent prognostic factor in oral squamous cell carcinoma (OSCC). However, its exact role is not yet elucidated, and a standardized scoring system is still necessary. The study aims to extensively review the literature data regarding the prognostic role of TB in OSCC. The results of TB are an independent prognostic factor of poor survival outcomes in OSCC. To date, the manual detection of hematoxylin and eosin-staining or pancytokeratin-immunostaining sections are the most commonly used methods. Between the several cut-offs, the two-tier system with five buds/field cut-offs provides better risk stratification. The prognostic role of the BD model in predicting survival outcomes was extensively validated; however, the inclusion of DOI, which is already a staging parameter, encouraged other authors to propose other models, integrating TB count with other adverse risk factors, such as the tumor–stroma ratio and tumor-infiltrated lymphocytes. The prognostic relevance of TB in OSCC highlights its evaluation in daily pathological practice. Therefore, the TB detection method and the TB scoring system should be validated based on tumor stage and site.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/308303

A Systematic Review on Nerve-Related Adverse Effects following Mandibular Nerve Block Anesthesia
INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH
Autore/i: Aquilanti, L.; Mascitti, M.; Togni, L.; Contaldo, M.; Rappelli, G.; Santarelli, A.
Classificazione: 1 Contributo su Rivista
Abstract: Inferior alveolar nerve (IAN) block injections are commonly used in clinical practice, but they are not free from complications. The aim of the present systematic review is to assess the nerve-related adverse effects of IAN block anesthesia. A structured and systematic search was performed on the major electronic databases (PubMed, Cochrane Library, Web of Science, Scopus and CINAHL) for studies published in English until 30 September 2021. A total of 131 articles were identified through database searching using combinations of keywords. Fifteen papers were included and assessed for eligibility. Overall, nerve damage following an IAN block anesthesia injection is a rare occurrence, probably due to the direct nerve trauma of the needle, a neurotoxic effect of the used anesthetic solution and/or a combination of them. From a medico-legal point of view, a balanced discussion prior to nerve block anesthesia should be pursued in order to avoid patients’ reluctance to undergo necessary dental treatment due to the remote eventuality of nerve injury.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/296646

Lymphovascular invasion as a prognostic tool for oral squamous cell carcinoma: a comprehensive review
INTERNATIONAL JOURNAL OF ORAL AND MAXILLOFACIAL SURGERY
Autore/i: Mascitti, M.; Togni, L.; Caponio, V. C. A.; Zhurakivska, K.; Bizzoca, M. E.; Contaldo, M.; Serpico, R.; Lo Muzio, L.; Santarelli, A.
Classificazione: 1 Contributo su Rivista
Abstract: Oral cancer is the most common malignancy of the head and neck region, characterized by a poor prognosis. Novel prognostic markers are needed to better stratify these patients. Lymphovascular invasion (LVI) has been included in the eighth edition of the AJCC Cancer Staging Manual as an additional prognostic factor, but its influence on the recurrence risk and lymph node metastasis is relatively understudied. This is a comprehensive review of the literature on the clinical and prognostic role of LVI in oral cancer. A relevant search of the PubMed, Scopus, and Web of Science databases yielded 29 articles that satisfied the inclusion criteria. Findings indicated that LVI is an independent negative prognostic factor in oral cancer patients and appears to be associated with cervical lymph node metastasis and loco-regional recurrence. Notably, in oral tongue cancer, survival outcomes progressively worsen when LVI is associated with other adverse pathological features, especially in the early stages. Therefore, these patients could benefit from elective neck dissection and/or adjuvant therapy. The high variability of LVI prevalence hinders the comparison of literature results. Several methodological limitations were found to be present in the collected articles, including the lack of a rigorous definition for LVI, the difficult detection in routine histological section, the presence of potential confounders, the retrospective nature, and an inadequate sample size in most studies. Therefore, it is necessary to conduct prognostic studies using standardized methods to define and quantify LVI.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/296731

Sviluppo di un modello prognostico morfologico per il carcinoma squamocellulare della lingua
Autore/i: Mascitti, Marco
Editore: Università Politecnica delle Marche
Classificazione: 8 Tesi di dottorato
Abstract: Il carcinoma squamocellulare della lingua (OTSCC) è una neoplasia caratterizzata da un elevato tasso di recidive e da una mortalità stabile da oltre 20 anni. L’ottava edizione del sistema di stadiazione dell’AJCC ha migliorato la capacità di stratificare i pazienti affetti da OTSCC. Ciononostante, la capacità predittiva di tale sistema deve essere migliorata per poter discriminare quei pazienti affetti da una malattia più aggressiva, rendendo necessaria la ricerca di nuovi marcatori prognostici. Lo scopo di questo lavoro è stato quello di studiare le principali caratteristiche morfologiche del tessuto tumorale, sviluppando un modello prognostico e un nomogramma al fine di migliorare la stratificazione dei pazienti affetti da OTSCC. È stato condotto uno studio di coorte retrospettivo su 211 pazienti con OTSCC. Sono stati valutati vari parametri istopatologici sezioni seriali da 4 μm colorate con ematossilina-eosina: il pattern d’invasione tumorale, l’invasione perineurale (PNI), l’invasione linfovascolare (LVI), il budding tumorale (BT), l’eosinofilia associata al tumore (TATE), il tumor-stroma ratio (TSR) e l’immunofenotipo. Gli endpoint esaminati sono stati la sopravvivenza specifica da malattia (DSS) e la sopravvivenza libera da malattia (DFS). L’analisi univariata di Cox ha evidenziato una riduzione della DSS nei soggetti che presentavano lo Stadio avanzato (p<0,001), la PNI multifocale (p<0,001) e peritumorale (p=0,003), la LVI (p<0,001), il BT (p<0,001), la scarsa TATE (p<0,001), il TSR basso (p=0,001) e l’immunofenotipo deserto (p<0,001). L’analisi multivariata e la regressione stepwise hanno permesso di costruire un modello prognostico impiegando età, Stadio, BT, LVI e immunofenotipo; tale modello ha mostrato gli indici di performance migliori. I risultati ottenuti suggeriscono che questo modello prognostico è un sistema semplice, economico e pratico per la valutazione degli OTSCC. Tuttavia sono necessari ulteriori studi di validazione su coorti più ampie.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/286953

Treatment‐related dysgeusia in oral and oropharyngeal cancer: A comprehensive review
NUTRIENTS
Autore/i: Togni, L.; Mascitti, M.; Vignigni, A.; Alia, S.; Sartini, D.; Barlattani, A.; Emanuelli, M.; Santarelli, A.
Classificazione: 1 Contributo su Rivista
Abstract: Oral cancer is the most common tumor of the head and neck region. Its management is based on surgical and systemic therapies. Taste disorders represent the most common side effect of these treatments; indeed, dysgeusia is noted by 70% of oral cancer patients. Despite survival remaining the primary endpoint of cancer patients, taste impairments can cause psychological distress. This comprehensive review describes the last decade’s knowledge from the literature regarding taste alterations in patients with oral and oropharyngeal squamous cell carcinoma. A total of 26 articles in English, including prospective, cross‐sectional, and case–control studies, and clinical trials were evaluated. Literature analysis shows that anti‐cancer treatments can destroy taste cells, decrease and alter their receptors, and interrupt nerve transmission. Furthermore, the tumour itself can destroy the oral mucosal lining, which encloses the taste buds. Dysgeusia typically occurs in 3– 4 weeks of treatments, and usually taste sensation is recovered within 3–12 months. However, some patients exhibit incomplete or no recovery, even several years later. Thus, dysgeusia can become a chronic issue and negatively influence patients’ quality of life, worsening their dysphagia and their nutritional status. Physicians should be focused on preventing oncological treatment‐related symptoms, offering the most suitable personalized support during therapy.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/293485

Bruxism in children and adolescents with down syndrome: A comprehensive review
MEDICINA
Autore/i: Luconi, E.; Togni, L.; Mascitti, M.; Tesei, A.; Nori, A.; Barlattani, A.; Procaccini, M.; Santarelli, A.
Classificazione: 1 Contributo su Rivista
Abstract: The role of bruxism in children and adolescents with Down syndrome, the most often diagnosed congenital syndrome, is still unclear. Therefore, this study aims to conduct a narrative review of the literature about bruxism in children and adolescents with Down syndrome to identify the prevalence, risk factors, and possible treatments of this disorder. Although an accurate estimate of its prevalence could not be inferred, it appears that bruxism is more prevalent in Down syndrome individuals rather than in the general pediatric population. No gender difference was observed, but a reduction in its prevalence was described with increasing age (around 12 years). The variability in the diagnostic techniques contributed to the heterogeneity of the literature data. Clinicopathological features of Down syndrome, such as muscle spasticity, oral breathing, and a predisposition to obstructive sleep apnea, may suggest a higher prevalence of bruxism in this patient group. Finally, given the paucity of studies on the management of bruxism in this population, it was not possible to outline a standard protocol for the non-invasive treatment of cases in which an observational approach is not sufficient.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/289892

Prognostic assessment of different methods for eosinophils detection in oral tongue cancer
JOURNAL OF ORAL PATHOLOGY & MEDICINE
Autore/i: Caponio, V. C. A.; Togni, L.; Zhurakivska, K.; Santarelli, A.; Arena, C.; Rubini, C.; Lo Muzio, L.; Troiano, G.; Mascitti, M.
Classificazione: 1 Contributo su Rivista
Abstract: Background: TATE has been proposed as a prognostic factor in oral cancer staging; however, the controversial literature data limit its application in the routine diagnosis. The aim of this study was to evaluate the prognostic value of TATE in patients with oral tongue cancer. The second aim was to identify any difference in the methods of eosinophil quantification or in the cutoff values reported in literature. Methods: Clinic-pathological data of 204 patients treated at “Ospedali Riuniti” Hospital, Ancona, Italy, were collected. Evaluation of TATE was performed on hematoxylin-and-eosin-stained slides and correlation with survival outcomes was evaluated. The number of eosinophils per square millimeter was evaluated by using two methods, namely density (TATE-1) and classical (TATE-2) methods. For each of the 2 methods tested, patients were stratified into two or three groups, according to the most used cutoff values reported in literature. Results: Regardless of the method of eosinophil quantification or the cutoff values used, patients with high TATE had a significantly better disease-specific survival. The density method (TATE-1) showed a better predictive performance, in particular when applying a single cutoff of 67 eosinophils/mm2, two cutoffs of 10 and 100 eosinophils/mm2, or two cutoffs of 50 and 120 eosinophils/mm2. Conclusion: The evaluation of TATE is simple, cost-effective, and easy to implement in daily practice with the aim of improving risk stratification of patients affected by oral tongue cancer. Results of prognostic performance analysis suggest using density (TATE-1) method as the standard approach to evaluate TATE in future studies, enhancing replicability.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/296647

Therapeutic potential of antibody‐drug conjugate‐based therapy in head and neck cancer: A systematic review
CANCERS
Autore/i: Perrotti, V.; Caponio, V. C. A.; Mascitti, M.; Lo Muzio, L.; Piattelli, A.; Rubini, C.; Capone, E.; Sala, G.
Classificazione: 1 Contributo su Rivista
Abstract: Background: Antibody‐drug conjugates (ADCs) are designed to deliver potent cytotoxic agents into tumor tissues. During the last two decades, a plethora of ADCs have been successfully developed and used for several indications, including hematologic and solid tumors. In this work, we systematically reviewed the progress in ADC development for the treatment of HNC. Methods: This review was registered in PROSPERO database. A comprehensive search was conducted following PRISMA guidelines and using PubMed, Scopus and Web of Science database. Results: In total, 19 studies were included. Due to the significant heterogeneity of the outcome measures, meta-analysis was not performed, and data were summarized in tables. HNC results are poorly represented in the cohorts of completed clinical trials; published data are mostly focused on safety evaluation rather than efficacy of ADCs. Conclusions: Although several novel agents against a wide range of different antigens were investigated, showing promising results at a preclinical level, most of the targets reported in this review are not specific for HNC; hence, the development of ADCs tailored for the HNC phenotype could open up new therapeutic perspectives. Moreover, the results from the present systematic review call attention to how limited is the application of current clinical trials in HNC.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/296733

Prognostic Relevance of Macrophage Phenotypes in High-grade Oral Tongue Squamous Cell Carcinomas
APPLIED IMMUNOHISTOCHEMISTRY AND MOLECULAR MORPHOLOGY
Autore/i: Agarbati, Silvia; Mascitti, Marco; Paolucci, Eleonora; Togni, Lucrezia; Santarelli, Andrea; Rubini, Corrado; Fazioli, Francesca
Classificazione: 1 Contributo su Rivista
Abstract: Tumor-associated macrophages (TAMs) are part of the tumor microenvironment, broadly divided into M1 and M2 phenotypes. M1 macrophages, commonly identified by staining the CD11c antigen, have an antitumour immunity role, while M2 macrophages, expressing the CD163 antigen, are involved in tumor progression. Little is known about M1 and M2 phenotypes in the context of the oral tongue squamous cell carcinomas (OTSCC), a subgroup of oral cancer with peculiar clinical behavior. This study evaluated the macrophage polarization in OTSCC specimens to examine their prognostic relevance. To this end, specimens from 71 OTSCC patients graded as G1 or G3 were investigated for CD11c and CD163 expression. Immunohistochemical staining of TAMs was evaluated in tumor nests, tumor inflammation area (TIA), and tumor stroma. To analyze the expression of CD11c and CD163, the percentage of positive cells was scored as 0 (negative), 1 (<10%), 2 (11% to 50%), 3 (51% to 80%), and 4 (>80%). The staining intensity was scored as 0 (negative), 1 (weak), 2 (moderate), and 3 (intense). Higher expression of both CD163 and CD11c macrophages in inflammation area positively correlated with G3 grade, both in extension and intensity. Focusing on G3 tumors, survival curves showed better disease-free survival in patients with high CD11c expression in the TIA. Presence of CD163 expression in TIA was associated with worse disease-free survival. This study evaluated, for the first time, the distribution of M1 and M2 macrophages in relation to the pathologic grade in OTSCC, highlighting the prognostic relevance of analyzing the localization of TAMs.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/286691

Oral manifestations of COVID-19 in hospitalized patients: A systematic review
INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH
Autore/i: Orilisi, G.; Mascitti, M.; Togni, L.; Monterubbianesi, R.; Tosco, V.; Vitiello, F.; Santarelli, A.; Putignano, A.; Orsini, G.
Classificazione: 1 Contributo su Rivista
Abstract: Background: COVID-19 disease first appeared in 2019 and quickly spread worldwide, causing a global pandemic. The oral cavity represents a target of SARS-CoV-2, and oral lesions are observed in both non-hospitalized and hospitalized patients. This systematic review aims to investigate the frequency of oral manifestations in COVID-19 hospitalized patients. Methods: An electronic search was conducted in PubMed, Scopus, and Web of Science databases, including articles published up to September 2021. The review protocol was based on PRISMA-P. The risk of bias of the studies was assessed using the Joana Briggs Institute. The certainty of evidence was assessed using the GRADE instrument. Results: Fifty-nine articles were included: 19 case reports, 17 case series, 2 case-control studies, 13 cross-sectional studies, 4 observational studies, and 4 retrospective studies. Oral ulcers, cheilitis, and tongue lesions were more common in patients before hospitalization, while perioral pressure ulcers, macroglossia, blisters, and oral candidiasis were more recurrent in patients during hospitalization. The first could be related directly to COVID-19, while the latter could be caused by medical devices, treatments, prone position, and immunological impairment. Conclusions: An accurate oral examination during the hospital admission of all confirmed COVID-19 cases is encouraged to recognize oral early manifestations and to apply appropriate treatments.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/293790

Pattern and localization of perineural invasion predict poor survival in oral tongue carcinoma
ORAL DISEASES
Autore/i: Caponio, V. C. A.; Troiano, G.; Togni, L.; Zhurakivska, K.; Santarelli, A.; Laino, L.; Rubini, C.; Lo Muzio, L.; Mascitti, M.
Classificazione: 1 Contributo su Rivista
Abstract: Background: Survival rate for oral tongue squamous cell carcinoma (OTSCC) is still poor and, despite Tumor–Node–Metastasis staging system has been recently updated, patients included under the same stage still show difference in prognosis. Perineural invasion (PNI) emerged to be an indicator of tumor aggressive behavior and unfortunate events. In this study, we investigate the clinic and prognostic value of PNI in a cohort of OTSCC patients. Methods: About 200 patients with OTSCC were retrospectively evaluated the presence of PNI. PNI was furtherly descripted as uni-/multifocal and as intra-/peritumoral. Disease-Specific and Relapse-Free Survival (DSS; RFS) were estimated; moreover, we included PNI in the current AJCC 8th Staging System, improving the prognostication model. Results: Perineural invasion was found in 40.5% of patients. Intratumoral PNI predicted patients at high risk of being diagnosed with lymph–node metastasis. Tumors with positive PNI reported a worse DSS (Hazard Ratio=1.878, p-value = 0.008). Moreover, patients exhibiting both multifocal intra- and peritumoral PNI reported poorest DSS (Hazard Ratio = 2.409, p-value = 0.010). Patients were reclassified in a new staging system in case of multifocal PNI, providing better stratification capacity. Conclusions: Perineural invasion might serve as an additional prognostic factor in OTSCC, and by integrating PNI in the staging system, further improvements in prognostication might be reached.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/296651

Nicotinamide N-Methyltransferase in Head and Neck Tumors: A Comprehensive Review
BIOMOLECULES
Autore/i: Togni, L; Mascitti, M; Sartini, D; Campagna, R; Pozzi, V; Salvolini, E; Offidani, A; Santarelli, A; Emanuelli, M
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/295330

Non-neoplastic jaw cysts: a 30-year epidemiological study of 2150 cases in the Italian population
BRITISH JOURNAL OF ORAL & MAXILLOFACIAL SURGERY
Autore/i: Aquilanti, L.; Mascitti, M.; Togni, L.; Rubini, C.; Nori, A.; Tesei, A.; Rappelli, G.; Santarelli, A.
Classificazione: 1 Contributo su Rivista
Abstract: Non-neoplastic jaw cyst (NJC) is one of the most common lesions in oral cavity, but there are only few detailed and extended epidemiological data based on the 2017 WHO classification. The aim of this study was to perform an epidemiological analysis of all NJCs treated from 1990 to 2019 at the Marche Polytechnic University, and to compare these data with those published in the literature. This retrospective study considered 2060 patients treated from 1990 to 2019. The NJCs were classified according to the 2017 WHO classification, and the main clinicopathological variables were analysed (sex, age, diagnosis, site of onset, size, and recurrences). Of 2150 total lesions, there were 2095 primary cysts and 55 recurrences; men are more frequently affected than women (M/F ratio of 1.73:1). The mean age of occurrence was 46.6 years, with a peak of frequency in the fifth decade. The mandible was more frequently involved than the maxilla, with a mean size of 1.9 cm. Radicular cyst was the most frequently diagnosed cyst (56.6%), followed by dentigerous cyst (23.4%) and odontogenic keratocyst (12.9%). This is the first epidemiological study on NJCs in the Italian population according to 2017 WHO classification.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/289893

Tumour-associated tissue eosinophilia (TATE) in oral squamous cell carcinoma: a comprehensive review
HISTOLOGY AND HISTOPATHOLOGY
Autore/i: Mascitti, Marco; Togni, Lucrezia; Rubini, Corrado; Troiano, Giuseppe; Lo Muzio, Lorenzo; Santarelli, Andrea
Classificazione: 1 Contributo su Rivista
Abstract: Oral squamous cell carcinoma is the most common head and neck malignancy, characterised by high invasive capacity, lymph node metastasis, and high recurrence rate. Among the morphological features of oral cancer, the tumour-associated tissue eosinophilia has gained growing interest in the last years. Eosinophils are a minor subpopulation of leukocytes, representing 1-3% of all circulating white blood cells. The presence of high levels of eosinophils is associated with several diseases, but their role in cancer pathophysiology is controversial. In particular, an uncertain and contradictory relationship exists between the exact role of tumour-associated tissue eosinophilia and oral cancer development. Many studies have shown that tumour-associated tissue eosinophilia increases both in the progression of oral potentially malignant disorders as well as in the grade and stage progression of oral cancer. Despite this, both negative and positive prognostic outcomes have been associated with eosinophil infiltration. The heterogeneous results may be partially due both to several methodological inconsistencies and to an incorrect interpretation of the physiological role of eosinophils. Therefore, further studies to elucidate the contribution of eosinophil infiltration are needed, focusing on the existence of eosinophil subpopulations regulated by the cancer immune microenvironment. Furthermore, the correct reporting of prognostic marker research is encouraged, in order to ensure the reproducibility and the comparability of the results from different studies.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/287288

Dental public health landscape: Challenges, technological innovation and opportunities in the 21st century and COVID-19 pandemic
INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH
Autore/i: Mascitti, M.; Campisi, G.
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/327391

A novel color-based segmentation method for the objective measurement of human masticatory performance
APPLIED SCIENCES
Autore/i: Aquilanti, L.; Scalise, L.; Mascitti, M.; Santarelli, A.; Napolitano, R.; Verdenelli, L.; Rappelli, G.
Classificazione: 1 Contributo su Rivista
Abstract: The aims of this study were to propose an automatic color-based segmentation method to separate mixed and unmixed colors of images that were derived from the application of the two-color chewing-gum mixing test and to determine the validity of this method in the assessment of masticatory performance (MP). Fifty young adults (mean age: 24.3 ± 2.7 years) were enrolled in the study. Each participant chewed a double-colored chewing gum for 5, 10, 20, 30, and 50 masticatory cycles. Boluses were collected and flattened. Both sides of each bolus were photographed, and images were processed using a novel k-means clustering method. The specimens corresponding to 20 masticatory cycles were re-analyzed by the same investigator in order to evaluate the intra-rater reliability and by a second investigator to assess the inter-rater reliability. To assess the test–retest reliability, 25% of the participants performed a second test with 20 chewing cycles. Each bolus was subjectively scored as either poorly, moderately, or highly mixed by an investigator to assess the construct validity. The percentage of mixed colors in the samples increased with an increase in the number of strokes. Significative differences were detected when varying from 5 to 10 strokes, from 10 to 20 strokes, and from 30 to 50 strokes (p < 0.05). The Pearson correlation coefficient explained these relations (r = 0.78, p < 0.05). The interclass correlation coefficient (ICC) showed a good correlation concerning both the intra-and inter-rater reliability (r = 0.85 and r = 0.77, respectively) and an excellent test–retest correlation (r = 0.93). The subjective assessment was coherent with the digital one. The proposed digital method was proved to be able to automatically quantify the percentage of the mixed color area by providing quantitative data with minimal human interaction.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/288050

Can Desensitizing Toothpastes Also Have an Effect on Gingival Inflammation? A Double-Blind, Three-Treatment Crossover Clinical Trial
INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH
Autore/i: Monterubbianesi, Riccardo; Sparabombe, Scilla; Tosco, Vincenzo; Profili, Fabia; Mascitti, Marco; Hosein, Andrell; Putignano, Angelo; Orsini, Giovanna
Classificazione: 1 Contributo su Rivista
Abstract: Background: Many desensitizing toothpastes exist commercially; however, few clinical trials have investigated their anti-inflammatory effects. This study aimed to evaluate the anti-inflammatory effect and patient appreciation of two toothpastes containing desensitizing agents: (1) a zinc-carbonate-hydroxyapatite nanoparticle (CHA) and (2) a calcium sodium phosphosilicate bioactive glass (CSPS). Methods: CHA and CSPS were compared with an anti-inflammatory and antibacterial herbal based toothpaste (HB). The aims were accomplished by comparing the following outcomes: (1) the reduction in plaque and bleeding score (Full Mouth Plaque Score (FMPS) and Full Mouth Bleeding Score (FMBS), respectively); (2) the antibacterial activity (AbA) of the toothpaste by saliva samples; (3) the patient appreciation score (Visual Analogue Scale; VAS). Clinical parameters were assessed at baseline and 14 days post-treatment. Results: The final sample consisted of 25 subjects, aged between 20 and 58 years. Although no differences in FMPS were reported (p > 0.05), both desensitizing toothpastes showed an improvement in FMBS. CSPS and HB recorded more AbA compared to CHA (p < 0.05). Moreover, HB resulted in a higher VAS score than both desensitizing toothpastes (p < 0.05). Conclusion: In conclusion, only CSPS displayed a similar anti-inflammatory effect compared to HB. Despite the low VAS score, CSPS could be considered as a valid and effective toothpaste in subjects with both dentin hypersensitivity and inflamed gums, highlighting its utility in clinical practice.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/285651

p53-Family Proteins in Odontogenic Cysts: An Immunohistochemical Study.
APPLIED IMMUNOHISTOCHEMISTRY AND MOLECULAR MORPHOLOGY
Autore/i: Mascitti, M; Togni, L; Balercia, A; Balercia, P; Rubini, C; Santarelli, A
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/263182

Dental care access and the elderly: What is the role of teledentistry? A systematic review
INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH
Autore/i: Aquilanti, L.; Santarelli, A.; Mascitti, M.; Procaccini, M.; Rappelli, G.
Classificazione: 1 Contributo su Rivista
Abstract: A high level of unmet oral health needs is very common among elderly people. In a society that is getting older and that has been hit so hard by the coronavirus pandemic, the development of new strategies aimed at enhancing general and oral health status should be crucial in order to promote healthy aging. The aim of this systematic review is to assess the feasibility of Teledentistry in communities or in a domiciliary setting where elderly people live. A structured and systematic research was performed on the major electronic databases for studies published in English until 30 June 2020: the PubMed, Cochrane Library, Web of Science, Scopus, and CINAHL databases. A total of 13 articles were identified through database searching using combinations of keywords. Out of 13 papers, eight abstracts were reviewed to assess if they were coherent with the aim of the study, and full texts were retrieved. After abstract reviews, seven articles were selected for closer inspection. Of these, six were assessed for eligibility. Four papers were aimed at assessing patients and health practitioners experiences about Teledentistry, three studies reported a cost analysis and cost description of Teledentistry in residential aged care facilities, and two studies investigated the feasibility and accuracy of Teledentistry for diagnosis dental pathology. The implementation of Teledentistry in residential aged care facilities and in home-assistance programs could be a viable tool for the management of oral care in people who cannot access dental care.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/289889

Biomarkers in head and neck oncology: From early diagnosis to tailored cancer therapy
The First Outstanding 50 Years of "Universita Politecnica delle Marche": Research Achievements in Life Sciences
Autore/i: Santarelli, A; Mascitti, M; Emanuelli, M; Procaccini, M
Editore: Springer International Publishing
Classificazione: 2 Contributo in Volume
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/285975

Differential expression of nicotinamide N-methyltransferase in primary and recurrent ameloblastomas and odontogenic keratocysts
EUROPEAN JOURNAL OF CLINICAL INVESTIGATION
Autore/i: Mascitti, M; Sartini, D; Togni, L; Pozzi, V; Rubini, C; Santarelli, A; Emanuelli, M
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/278206

Impact of elderly masticatory performance on nutritional status: An observational study
MEDICINA
Autore/i: Aquilanti, L.; Alia, S.; Pugnaloni, S.; Coccia, E.; Mascitti, M.; Santarelli, A.; Limongelli, L.; Favia, G.; Mancini, M.; Vignini, A.; Rappelli, G.
Classificazione: 1 Contributo su Rivista
Abstract: Background and Objectives: Masticatory limitations on the dietary habits of edentulous subjects restrict their access to adequate nutrition, exposing them to a greater risk of protein energy malnutrition. The aim of this study is to verify the existence of an association between Masticatory Performance (MP) and nutritional changes in the elderly. Materials and Methods: 76 participants were enrolled. MP testing was performed using the two-color chewing gum mixing test. The system used reveals the extent to which the two differently colored chewing gums mix, and allows discrimination between different MPs. The assessment of the participants’ nutritional statuses was carried out through a food interview. Anthropometric parameters were collected, and bioimpedance analysis was performed. Results: Mean MP was 0.448 ± 0.188. No statistically significant differences were detected between male and female subjects (p > 0.05). According to the Body Mass Index (BMI), obese patients had a lower MP than overweight and normal weight subjects (0.408 ± 0.225, 0.453 ± 0.169 and 0.486 ± 0.181, respectively). MP values were lower both in male and female subjects with a waist circumference above the threshold than those below it (0.455 ± 0.205 vs. 0.476 ± 0.110, respectively, in males and 0.447 ± 0.171 vs. 0.501 ± 0.138, respectively, in females). No relationship was noticed between MP and bioimpedance parameters (p > 0.05). Conclusions: A statistically significant relation was observed between MP and the number of missing teeth. A reduced MP could worsen nutritional parameters. A reduced MP did not seem to negatively affect bioimpedance parameters.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/278096

The immune phenotype of tongue squamous cell carcinoma predicts early relapse and poor prognosis
CANCER MEDICINE
Autore/i: Troiano, G.; Rubini, C.; Togni, L.; Caponio, V. C. A.; Zhurakivska, K.; Santarelli, A.; Cirillo, N.; Lo Muzio, L.; Mascitti, M.
Classificazione: 1 Contributo su Rivista
Abstract: Background: In patients with squamous cell carcinoma of the oral tongue (OTSCC), current tumor node metastasis staging system fails to identify at-risk patients associated with early relapse and poor prognosis despite complete surgical resection. Given the importance of tumor-infiltrating lymphocytes (TILs) in the development of cancers, here we investigated the prognostic significance of the immune phenotype in OTSCC. Methods: Hematoxylin-eosin stained sections of OTSCCs from 211 patients were evaluated. Cancers were classified as (a) immune-inflamed when TILs were found next to tumor cell nests; (b) immune-excluded when TILs were found in the stroma, outside the tumor; and (c) immune-desert for tumors lacking lymphocyte infiltrate. The prognostic significance of these immune phenotypes classes was investigated. Data were further validated on an independent cohort from The Cancer Genome Atlas database. Results: Immune-desert phenotype was the least represented group of OTSCCs in our cohort (11.8%) and served as an independent prognostic factor. Patients with immune-desert tumors exhibited worse disease-specific survival (HR = 2.673; [CI: 95% 1.497-4.773]; P =.001), overall survival (HR = 2.591; [CI: 95% 1.468-4.572]; P =.001), and disease-free survival (HR = 2.313; [CI: 95% 1.118-4.786]; P =.024) at multivariate analysis. Conclusions: We identified a specific subgroup of OTSCCs with poor prognosis. Tumor-infiltrating lymphocytes density and localization could serve as an integrative parameter to the current staging system and inform the selection of most appropriate treatments. In particular, the tumor immune phenotype could improve the stratification of patients with more aggressive disease.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/287287

Addition of the tumour–stroma ratio to the 8th edition American Joint Committee on Cancer staging system improves survival prediction for patients with oral tongue squamous cell carcinoma
HISTOPATHOLOGY
Autore/i: Mascitti, M.; Zhurakivska, K.; Togni, L.; Caponio, V. C. A.; Almangush, A.; Balercia, P.; Balercia, A.; Rubini, C.; Lo Muzio, L.; Santarelli, A.; Troiano, G.
Classificazione: 1 Contributo su Rivista
Abstract: Aims: One of the objectives of current research is to customise the treatment of cancer patients. The achievement of this objective requires stratification of patients based on the most significant prognostic factors. The aims of this study were to evaluate the prognostic value of the tumour–stroma ratio (TSR), defined as the proportion of tumour cells relative to surrounding stroma, in patients with oral tongue squamous cell carcinoma (OTSCC), and to develop a prognostic nomogram based on the most significant clinicopathological features. Methods and Results: Clinicopathological data of 211 patients treated at ‘Ospedali Riuniti’ General Hospital (Ancona, Italy) for OTSCC were collected. One hundred and thirty-nine patients were restaged according to the 8th edition American Joint Committee on Cancer (AJCC) staging system. Evaluation of the TSR was performed on haematoxylin and eosin-stained slides, and correlation with survival outcomes was evaluated. In addition, with the aim of integrating the independent value of the TSR with the 8th edition AJCC staging system, a prognostic nomogram for OTSCC has been developed. OTSCC with a low TSR (i.e. a high proportion of stroma and a low proportion of tumour cells) was shown to have negative prognostic value in terms of disease-specific survival, with a hazard ratio (HR) of 1.883 and a 95% confidence interval (CI) of 1.033-3.432 (P = 0.039), and overall survival (HR = 1.747, 95% CI 0.967-3.154; P = 0.044), independently of other histological and clinical parameters. For the cohort of 139 patients restaged according to the 8th edition AJCC staging system, variables correlating with a poor prognosis were: the TSR, perineural invasion, and sex. The nomogram built on these parameters showed good predictive capacity, outperforming the 8th edition AJCC staging system in stratifying disease-specific survival in OTSCC patients. Conclusions: Including the TSR in the predictive model could improve risk stratification of OTSCC patients and aid in making treatment decisions.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/287285

Histological features and survival in young patients with HPV-negative oral squamous cell carcinoma
ORAL DISEASES
Autore/i: Mascitti, M.; Tempesta, A.; Togni, L.; Capodiferro, S.; Troiano, G.; Rubini, C.; Maiorano, E.; Santarelli, A.; Favia, G.; Limongelli, L.
Classificazione: 1 Contributo su Rivista
Abstract: Objectives: The frequency of oral squamous cell carcinoma in young adults has increased in the last decades, and there are conflicting results in literature about its prognosis in young subjects. The aim of this study was to analyse the clinical and pathological features of oral squamous cell carcinoma in a cohort of young adults in order to investigate the presence of new independent prognostic markers. Materials and Methods: Only HPV-negative young patients (under 40-year-old) affected by oral squamous cell carcinoma were considered in this study. Clinical and pathological data were collected. Patients were re-staged according to the 8th edition of AJCC. Results: Overall, 66 patients were considered in this study. Perineural invasion significant correlated with both 7th and 8th edition of AJCC, and lymphovascular invasion (p-value <.05). The multivariate survival analysis showed that patients with perineural invasion had a significant worse prognosis (HR = 6.384 95% C.I. 1.304–31.252; p-value =.022). Conclusions: Perineural invasion emerged as an independent prognostic factor for disease-specific survival in young patients with oral squamous cell carcinoma. Furthermore, the evaluation of this parameter is simple, inexpensive and can be used to augment the risk stratification of oral cancer based on the 8th edition of AJCC.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/287286

Odontogenic tumours: a 25-year epidemiological study in the Marche region of Italy
EUROPEAN ARCHIVES OF OTO-RHINO-LARYNGOLOGY
Autore/i: Mascitti, M.; Togni, L.; Troiano, G.; Caponio, V. C. A.; Sabatucci, A.; Balercia, A.; Rubini, C.; Lo Muzio, L.; Santarelli, A.
Classificazione: 1 Contributo su Rivista
Abstract: Purpose: Epidemiological data of odontogenic tumours (OT) are conflicting, with significant differences among the countries. This study aims to evaluate incidence and prevalence of OTs in the Marche population in a period of 25 years, according to 4th Edition of WHO Classification. Methods: In this study, only patients of Marche region treated for OTs were considered. Data were retrieved from Institute of Pathology, Marche Polytechnic University, Italy. Because this is the only tertiary referral centre for Head and Neck pathology within Marche region, the patient sample could be considered well representative of this area. From each case, age, sex, site, diagnosis and relapses were recorded. Results: Overall, 100 patients were treated for OTs from 1994 to 2018 in Marche region. The annual incidence rate ranged from 0.13 to 0.39 per 100,000, while life prevalence was 6.50 per 100,000. Mean age of onset for primary OTs was 49.7 ± 20.1 years. Twenty-seven patients developed recurrences, showing a mean age of 54 ± 19.7 years and a mean recurrence time of 51.2 ± 34 months. Conclusion: This is the first epidemiological study on OTs in Italian population according to 4th Edition of WHO Classification. Although limited in their retrospective nature, these findings could accurately estimate epidemiology of OTs in Italy.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/288135

Computational analysis of TP53 mutational landscape unveils key prognostic signatures and distinct pathobiological pathways in head and neck squamous cell cancer
BRITISH JOURNAL OF CANCER
Autore/i: Caponio, V. C. A.; Troiano, G.; Adipietro, I.; Zhurakivska, K.; Arena, C.; Mangieri, D.; Mascitti, M.; Cirillo, N.; Lo Muzio, L.
Classificazione: 1 Contributo su Rivista
Abstract: Background: Mutations of the tumour-suppressor gene TP53 are the most frequent somatic genomic alterations in head and neck squamous cell carcinoma (HNSCC). However, it is not yet clear whether specific TP53 mutations bear distinct clinical and pathophysiological significance in different HNSCC subgroups. Methods: A systematic bioinformatics appraisal of TP53 mutations was performed on 415 HNSCC cases available on The Cancer Genome Atlas (TCGA). The following features were analysed and correlated with known clinicopathological variables: mutational profile of TP53, location (within secondary structure and predicted domains of p53 protein) and well-known hotspot mutations. Interactome–genome–transcriptome network analysis highlighted different gene networks. An algorithm was generated to develop a new prognostic classification system based on patients’ overall survival. Results: TP53 mutations in HNSCCs exhibited distinct differences in different anatomical sites. The mutational profile of TP53 was an independent prognostic factor in HNSCC. High risk of death mutations, identified by our novel classification algorithm, was an independent prognostic factor in TCGA HNSCC database. Finally, network analysis suggested that distinct p53 molecular pathways exist in a site- and mutation-specific manner. Conclusions: The mutational profile of TP53 may serve as an independent prognostic factor in HNSCC patients, and is associated with distinctive site-specific biological networks.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/296730

Medication-related osteonecrosis of jaws (MRONJ) prevention and diagnosis: Italian consensus update 2020
INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH
Autore/i: Campisi, G.; Mauceri, R.; Bertoldo, F.; Bettini, G.; Biasotto, M.; Colella, G.; Consolo, U.; Di Fede, O.; Favia, G.; Fusco, V.; Gabriele, M.; Lo Casto, A.; Lo Muzio, L.; Marciano, A.; Mascitti, M.; Meleti, M.; Mignogna, M. D.; Oteri, G.; Panzarella, V.; Romeo, U.; Santarelli, A.; Vescovi, P.; Marchetti, C.; Bedogni, A.
Classificazione: 1 Contributo su Rivista
Abstract: The Medication-Related Osteonecrosis of Jaws (MRONJ) diagnosis process and its prevention play a role of great and rising importance, not only on the Quality of Life (QoL) of patients, but also on the decision-making process by the majority of dentists and oral surgeons involved in MRONJ prevention (primary and secondary). The present paper reports the update of the conclusions from the Consensus Conference—held at the Symposium of the Italian Society of Oral Pathology and Medicine (SIPMO) (20 October 2018, Ancona, Italy)—after the newest recommendations (2020) on MRONJ were published by two scientific societies (Italian Societies of Maxillofacial Surgery and Oral Pathology and Medicine, SICMF and SIPMO), written on the inputs of the experts of the Italian Allied Committee on ONJ (IAC-ONJ). The conference focused on the topic of MRONJ, and in particular on the common practices at risk of inappropriateness in MRONJ diagnosis and therapy, as well as on MRONJ prevention and the dental management of patients at risk of MRONJ. It is a matter of cancer and osteometabolic patients that are at risk since being exposed to several drugs with antiresorptive (i.e., bisphosphonates and denosumab) or, more recently, antiangiogenic activities. At the same time, the Conference traced for dentists and oral surgeons some easy applicable indications and procedures to reduce MRONJ onset risk and to diagnose it early. Continuous updating on these issues, so important for the patient community, is recommended.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/289890

Analysis of M1 and M2 tumor associated macrophages in tongue squamous cell carcinomas.
Front. Physiol. Conference Abstract: 5th National and 1st International Symposium of Italian Society of Oral Pathology and Medicine
Autore/i: Agarbati1, Silvia; Mascitti, Marco; Campisi, Giuseppina; LO MUZIO, Lorenzo; Rubini, Corrado; Fazioli, Francesca
Classificazione: 4 Contributo in Atti di Convegno (Proceeding)
Abstract: Tumor associated macrophages (TAMs) are the dominant leukocyte population found in the tumor microenvironment and their mobilization into tumor tissues is a critical event in tumor initiation, growth, and development. TAMs are a heterogeneous population of innate myeloid cells and take on various phenotypes depending on the context of the molecular stimuli from the microenvironment. Basically, there are two main recognized categories: classically-activated pro-inflammatory M1 macrophages (expressing the CD11c antigen) and alternatively-activated anti-inflammatory M2 macrophages (expressing the CD163 antigen). Although TAMs have been detected in head and neck cancers, little is known about their phenotype in the context of the tongue squamous cell carcinoma (TSCC), which have the highest incidence in maxillofacial malignant tumors. The aim of the present retrospective study was to characterize the macrophage polarization in low grade (G1) and high grade (G3) TSCC, and to examine the importance of their relative localization (tumor stroma, inflammation area or tumor nest) on tumor-promoting capabilities and tumor-prognostic relevance.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/279456

Evaluation of Echinophora Tenuifolia L. Extracts on HSC-2 Cell Line
PROCEEDINGS
Autore/i: Arena, Claudia; Vairano, Marco; Mascitti, Marco; Santarelli, Andrea; Dioguardi, Mario; Zhurakivska, Khrystyna
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/272712

Expression of p53-family members in odontogenic cysts
FRONTIERS IN PHYSIOLOGY
Autore/i: Mascitti, Marco; Rubini, Corrado; Procaccini, Maurizio; Lo Muzio, Lorenzo; Santarelli, Andrea
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/272726

Ameloblastic Fibrosarcoma: Report of a New Case
PROCEEDINGS
Autore/i: Tesei, A; Mascitti, M; Mastrangelo, F; Panzarella, V; Nori, A; Santarelli, A
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/272710

TIMELESS in Head and Neck Squamous Cell Carcinoma: A Systematic Review
PROCEEDINGS
Autore/i: Saracino, P; Arena, C; Mascitti, M; Santarelli, A; Panzarella, V; Lo Russo, L
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/272713

Salivary molecular diagnostics in ectodermal dysplasia
FRONTIERS IN PHYSIOLOGY
Autore/i: Torelli, Francesco; Mascitti, Marco; Panzarella, Vera; Lo Muzio, Lorenzo; Santarelli, Andrea; Armeni, Tatiana
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/272733

Recurrent Oral Ulcers: current and future therapeutic approaches
FRONTIERS IN PHYSIOLOGY
Autore/i: Mascitti, Marco; Santarelli, Andrea
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/272722

Odontogenic Cysts: A 30-Year Retrospective Clinicopathological Study
PROCEEDINGS
Autore/i: Mascitti, Marco; Togni, Lucrezia; Lo Muzio, Lorenzo; Campisi, Giuseppina; Mazzoni, Federico; Santarelli, Andrea
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/272714

p63 Expression in Solitary and Syndromic Odontogenic Keratocysts: An Immunohistochemical Study
PROCEEDINGS
Autore/i: Luconi, E; Togni, L; Giannatempo, G; Caponio, V; Mascitti, M; Santarelli, A
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/272717

Noma: a possible reappraisal in western countries
FRONTIERS IN PHYSIOLOGY
Autore/i: Pirani, Filippo; Mascitti, Marco; Ciavarella, Domenico; Caponio, Vito Carlo Alberto; Procaccini, Maurizio; Santarelli, Andrea
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/272728

New markers for odontogenic lesions: role of the Nicotinamide N-methyltransferase
FRONTIERS IN PHYSIOLOGY
Autore/i: Luconi, E; Mascitti, M; Giannatempo, G; Lo Muzio, L; Sartini, D; Emanuelli, M
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/272912

Analysis of immune-phenotype in squamous cells carcinoma of the tongue
FRONTIERS IN PHYSIOLOGY
Autore/i: Mascitti, Marco; Troiano, Giuseppe; Mauceri, Rodolfo; Lo Muzio, Lorenzo; Rubini, Corrado; Santarelli, Andrea
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/272723

Predicting Death in Patients with Squamous Cell Carcinoma of the Tongue
PROCEEDINGS
Autore/i: Caponio, V; Troiano, G; Mascitti, M; Santarelli, A; Mauceri, R; Lo Muzio, L
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/272718

Adenoid Cystic Carcinoma of Salivary Gland: An Immunohistochemical Study
PROCEEDINGS
Autore/i: Togni, Lucrezia; Mascitti, Marco; Rubini, Corrado; Mauceri, Rodolfo; Lo Muzio, Lorenzo; Santarelli, Andrea
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/272715

Spiderimplant: an innovative implantological approach to the treatment of atrophic maxilla
FRONTIERS IN PHYSIOLOGY
Autore/i: Coccia, Erminia; Zanna, Massimo; Mascitti, Marco; Santarelli, Andrea; Lo Muzio, Lorenzo; Procaccini, Maurizio
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/272724

Development of a Prognostic Model for Tongue Squamous Cell Carcinoma
PROCEEDINGS
Autore/i: Troiano, G; Zhurakivska, K; Mascitti, M; Santarelli, A; Campisi, G; Lo Muzio, L
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/272711

Survivin and oral squamous cell carcinoma: a review
FRONTIERS IN PHYSIOLOGY
Autore/i: Pirani, Filippo; Mascitti, Marco; Mastrangelo, Filiberto; Adipietro, Iolanda; Procaccini, Maurizio; Santarelli, Andrea
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/272730

Chondrosarcoma of the maxilla: a case report and literature review
FRONTIERS IN PHYSIOLOGY
Autore/i: Palini, Simone; Acquilanti, Luca; Togni, Lucrezia; Montanari, Luca; Mascitti, Marco; Piemontese, Matteo
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/272729

Anorexia, Oral Health and Antioxidant Salivary System: A Clinical Study on Adult Female Subjects.
DENTISTRY JOURNAL
Autore/i: Mascitti, M; Coccia, E; Vignini, A; Aquilanti, Luca; Santarelli, A; Salvolini, E; Sabbatinelli, J; Mazzanti, L; Procaccini, M; Rappelli, G
Classificazione: 1 Contributo su Rivista
Abstract: The aim of this study was to compare the oral health status and salivary antioxidant system between patients diagnosed with anorexia nervosa (AN) and healthy controls. A total of 25 female AN patients and 25 matched healthy controls were enrolled. Clinical parameters and saliva samples were collected for each patient. Two questionnaires to investigate oral health and hygiene were administered. Superoxide Dismutase (SOD) activity and High Reactive Oxygen Species (hROS) were evaluated. Salivary concentration of SOD was significantly higher in subjects with AN compared with control group (1.010 ± 0.462 vs. 0.579 ± 0.296 U/mL; p = 0.0003). No significant differences between groups were identified for hROS (233.72 ± 88.27 vs. 199.49 ± 74.72; p = 0.15). Data from questionnaires indicated that, although most of the patients recognized the oral hygiene importance in maintaining a good oral health, more than half of them had poor oral hygiene. Altered biochemical composition of saliva in patients with AN could be interpreted as an effective defence mechanism against oxidative stress. Moreover, despite the discrepancy between clinical findings and perception of the oral health in AN population arose, the quality of life of these patients appears not to be significantly affected by their dental condition.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/267618

Analysis of oral mucosa erosive-ulcerative lesions by reflectance confocal microscopy
JOURNAL OF BIOLOGICAL REGULATORS & HOMEOSTATIC AGENTS
Autore/i: Romano, A.; Santarelli, A.; Lajolo, C.; Della Vella, F.; Mascitti, M.; Serpico, R.; Contaldo, M.
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/270933

Noma: a reappraisal in Western countries - are HIV-negative immunocompetent adult patients safe?
JOURNAL OF BIOLOGICAL REGULATORS & HOMEOSTATIC AGENTS
Autore/i: Mascitti, M; Barlattani, A; Togni, L; Sampalmieri, F; Favia, G; Lo Muzio, L; Santarelli, A.
Classificazione: 1 Contributo su Rivista
Abstract: We present 3 cases of Noma occurred in HIV-negative immunocompetent Caucasian women in different clinical conditions, affected by some degree of malnutrition and poor oral health status. Each of these patients presented with an oral ulcer, characterized by non-specific histology and microbiological culture. Diagnosis of Noma was based on clinical findings and a proper therapy was undertaken. Antibiotic and antifungal therapy was combined with the use of nasogastric nutrition, oral hygiene measures and wound healing treatment. The lesions healed, and clinical conditions improved in all the patients. No recurrence was observed during follow-up period.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/267884

Association between denture stomatitis, candida species and diabetic status
JOURNAL OF BIOLOGICAL REGULATORS & HOMEOSTATIC AGENTS
Autore/i: Contaldo, M.; Romano, A.; Mascitti, M.; Fiori, F.; Della Vella, F.; Serpico, R.; Santarelli, A.
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/270934

Large cell neuroendocrine carcinoma of the submandibular gland: a case report and literature review
PATHOLOGICA
Autore/i: Mascitti, Marco; Luconi, Elisa; Togni, Lucrezia; Rubini, Corrado
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/269424

Unusual Conditions Impairing Saliva Secretion: Developmental Anomalies of Salivary Glands.
FRONTIERS IN PHYSIOLOGY
Autore/i: Togni, Lucrezia; Mascitti, Marco; Santarelli, Andrea; Maria, Contaldo; Romano, Antonio; Rosario, Serpico; Rubini, Corrado
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/267886

Analysis of nicotinamide N-methyltransferase in oral malignant melanoma and potential prognostic significance
MELANOMA RESEARCH
Autore/i: Mascitti, M; Santarelli, A; Sartini, D; Rubini, C; Colella, G; Salvolini, E; Ganzetti, G; Offidani, A; Emanuelli, M
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/263183

Beyond Head and Neck Cancer: The Relationship Between Oral Microbiota and Tumour Development in Distant Organs.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
Autore/i: Mascitti, M; Togni, L; Troiano, G; Caponio, Vca; Gissi, Db; Montebugnoli, L; Procaccini, M; Lo Muzio, L; Santarelli, A
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/267885

Overexpression of nicotinamide N-methyltransferase in HSC-2 OSCC cell line: effect on apoptosis and cell proliferation
CLINICAL ORAL INVESTIGATIONS
Autore/i: Seta, R; Mascitti, M; Campagna, R; Sartini, D; Fumarola, S; Santarelli, A; Giuliani, M; Cecati, M; Muzio, Ll; Emanuelli, M
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/259054

High PD-L1 expression in the tumour cells did not correlate with poor prognosis of patients suffering for oral squamous cells carcinoma: A meta-analysis of the literature.
CELL PROLIFERATION
Autore/i: Troiano, G; Caponio, Vca; Zhurakivska, K; Arena, C; Pannone, G; Mascitti, M; Santarelli, A; Lo Muzio, L
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/263181

Imatinib-related hyperpigmentation of oral mucosa: Case report and literature review
JOURNAL OF DENTAL SCIENCES
Autore/i: Mascitti, M.; Luconi, E.; Togni, L.; Barlattani, A.; Santarelli, A.
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/270931

Expression of nicotinamide N-methyltransferase in oral malignant melanoma
Atti del 15° Congresso Nazionale della Federazione Italiana Scienze della Vita (FISV), Roma, 18-21 Settembre 2018
Autore/i: Mascitti, M; Santarelli, A; Rubini, C; Salvolini, E; Pompei, Veronica; Sartini, D; Emanuelli, M
Classificazione: 4 Contributo in Atti di Convegno (Proceeding)
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/259339

Spider Zygoma: a new implant rehabilitation technique for atrophic maxilla.
JOURNAL OF BIOLOGICAL REGULATORS & HOMEOSTATIC AGENTS
Autore/i: Zanna, M; Mascitti, M; Coccia, E; Lo Muzio, L; Santarelli, A.
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/263178

Peripheral odontogenic myxoma: Report of two new cases with a critical review of the literature.
THE OPEN DENTISTRY JOURNAL
Autore/i: Mascitti, M; Togni, L; Pirani, F; Rubini, C; Santarelli, A
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/267619

Tongue “heart” ulcer from bisphosphonate
JOURNAL OF DENTAL SCIENCES
Autore/i: Mascitti, M; Santarelli, A
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/263180

American Joint Committee on Cancer staging system 7th edition versus 8th edition: any improvement for patients with squamous cell carcinoma of the tongue?
ORAL SURGERY, ORAL MEDICINE, ORAL PATHOLOGY AND ORAL RADIOLOGY
Autore/i: Mascitti, M; Rubini, C; De Michele, Francesco; Balercia, P; Girotto, R; Troiano, G; Lo Muzio, L; Santarelli, A
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/263179

Survivin-Based Treatment Strategies for Squamous Cell Carcinoma
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Autore/i: Santarelli, A; Mascitti, M; Lo Russo, L; Sartini, D; Troiano, G; Emanuelli, M; Lo Muzio, L
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/259050

An overview on current non-invasive diagnostic devices in oral oncology
FRONTIERS IN PHYSIOLOGY
Autore/i: Mascitti, Marco; Orsini, Giovanna; Tosco, Vincenzo; Monterubbianesi, Riccardo; Balercia, Andrea; Putignano, Angelo; Procaccini, Maurizio; Santarelli, Andrea
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/262141

Cystic lesions of the jaws: a retrospective clinicopathologic study of 2030 cases.
ORAL SURGERY, ORAL MEDICINE, ORAL PATHOLOGY AND ORAL RADIOLOGY
Autore/i: Lo Muzio, L; Mascitti, M; Santarelli, Andrea; Rubini, Corrado; Bambini, Fabrizio; Procaccini, Maurizio; Bertossi, D; Albanese, M; Bondì, V; Nocini, Pf
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/250009

Nuclear Survivin as a Prognostic Factor in Squamous-Cell Carcinoma of the Oral Cavity
APPLIED IMMUNOHISTOCHEMISTRY AND MOLECULAR MORPHOLOGY
Autore/i: Santarelli, Andrea; Mascitti, M; Rubini, Corrado; Bambini, Fabrizio; Giannatempo, G; Lo Russo, L; Sartini, Davide; Emanuelli, Monica; Procaccini, Maurizio; Lo Muzio, L.
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/236729

Odontogenic tumors: a retrospective clinicopathological study from two Italian centers.
PATHOLOGICA
Autore/i: Rubini, C; Mascitti, M; Santarelli, A; Tempesta, Antonio; Limongelli, Luca; Favia, G; Maiorano, E.
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/255193

Overexpression of Nicotinamide N-Methyltransferase in HSC-2 OSCC cell line: effect on apoptosis and cell proliferation
MINERVA STOMATOLOGICA
Autore/i: Giannatempo, G; Mascitti, M; Di Fede, O; Mauceri, R; Santarelli, Andrea; Seta, Riccardo; Sartini, Davide; Campagna, Roberto; Emanuelli, Monica; Lo Muzio, L.
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/237199

Validation of "Liverpool sicca index" on a sample of helderly patients.
MINERVA STOMATOLOGICA
Autore/i: Mascitti, M; Addesa, A; Bambini, F; Rappelli, G; Sampalmieri, F; Santarelli, A; Procaccini, M.
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/262513

Expression of p73 and TRAIL in odontogenic cysts and tumors.
JOURNAL OF ORAL SCIENCE
Autore/i: Mascitti, M; Santarelli, Andrea; Zizzi, A; Procaccini, Maurizio; Lo Muzio, L; Rubini, Corrado
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/247281

Oral ulcer by Sphingomonas paucimobilis: first report.
INTERNATIONAL JOURNAL OF ORAL AND MAXILLOFACIAL SURGERY
Autore/i: Santarelli, Andrea; Mascitti, M; Galeazzi, R; Marziali, A; Busco, F; Procaccini, Maurizio
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/237887

Oral health related quality of life (OHRQoL) in helderly population.
MINERVA STOMATOLOGICA
Autore/i: Santarelli, A; Mosca, M; Mascitti, M; Giuliani, M; di fede, O; Sampalmieri, F; Sabatucci, A; Procaccini, M.
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/262512

Active inflammatory biomarkers in oral lichen planus
INTERNATIONAL JOURNAL OF IMMUNOPATHOLOGY AND PHARMACOLOGY
Autore/i: Santarelli, Andrea; Mascitti, Marco; Rubini, Corrado; Bambini, Fabrizio; Zizzi, Antonio; Offidani, Annamaria; Ganzetti, Giulia; Laino, Luigi; Cicciù, Marco; Lo Muzio, Lorenzo
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/234527

Glandular odontogenic cyst: review of literature and report of a new case with cytokeratin-19 expression.
THE OPEN DENTISTRY JOURNAL
Autore/i: Mascitti, M.; Santarelli, Andrea; Sabatucci, Antonio; Procaccini, Maurizio; Lo Muzio, L.; Zizzi, Antonio; Rubini, Corrado
Classificazione: 1 Contributo su Rivista
Abstract: The glandular odontogenic cyst (GOC) was a rare jawbone cyst described in 1988 as a distinct entity. This lesion can involve either jaw, and the anterior region of the mandible was the most commonly affected area. Clinical and radiographic findings were not specific, and the diagnosis of GOC can be extremely difficult due to the rarity of this lesion. The cyst presented a wall constituted by fibrous connective tissue and was lined by a non-keratinized stratified squamous epithelium of variable thickness. Large areas of the lining epithelium presented cylinder cells, sometimes ciliated. A variable amount of mucina was occasionally noted. Due to the strong similarities, this cyst can be easily misdiag-nosed as a central mucoepidermoid carcinoma (CMEC). Immunohistochemistry may be an aid in diagnosis; in fact has been demonstrated that there were differences in the expression of cytokeratins (CK) in GOC and CMEC. In this study, we reported a new case of GOC in a 38 year female patient. In addition, we carried out a review of 110 previous cases reported in literature.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/154750

Osteopontin, osteocalcin and OB-cadherin expression in Synthetic nanohydroxyapatite vs bovine hydroxyapatite cultured Osteoblastic-like cells.
JOURNAL OF BIOLOGICAL REGULATORS & HOMEOSTATIC AGENTS
Autore/i: Santarelli, Andrea; Mascitti, M; Orsini, Giovanna; Memè, L; Rocchetti, R; Tiriduzzi, P; Sampalmieri, Francesco; Putignano, Angelo; Procaccini, Maurizio; Lo Muzio, L; Bambini, Fabrizio
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/197705

Glandular odontogenic cyst: report of a new case with cytokeratin 19 expression
ANNALI DI STOMATOLOGIA
Autore/i: Mascitti, M.; Santarelli, Andrea; Di Fede, O.; Rubini, Corrado; Lo Muzio, L.
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/122664

Survivin detection in saliva samples from patients with oral squamous cell carcinoma
Atti del 20° Congresso Nazionale del Collegio dei Docenti di Odontoiatria
Autore/i: Mascitti, M.; Santarelli, Andrea; Emanuelli, Monica; Bambini, Fabrizio; Rappelli, Giorgio Giulio Lorenzo; Quaranta, Alessandro; Lo Muzio, L.; Procaccini, Maurizio
Classificazione: 4 Contributo in Atti di Convegno (Proceeding)
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/126273

Detection Level of Salivary Survivin in Patients with OSCC
JOURNAL OF CARCINOGENESIS & MUTAGENESIS
Autore/i: Santarelli, Andrea; Mascitti, M.; Lo Russo, L.; Colella, G.; Giannatempo, G.; Bambini, Fabrizio; Emanuelli, Monica; Procaccini, Maurizio; Lo Muzio, L.
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/128472

A case of recurrent aphthous stomatitis that had lead to coeliac disease diagnosis
Atti del XX Congresso Nazionale del Collegio dei Docenti di Odontoiatria
Autore/i: Sampalmieri, Francesco; Agostini, R.; Mascitti, M.; Orsini, Giovanna; Santarelli, Andrea; Bambini, Fabrizio; Giannatempo, G.; Lo Muzio, L.; Procaccini, Maurizio
Classificazione: 4 Contributo in Atti di Convegno (Proceeding)
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/126275

RNA-mediated gene silencing NNMT decreases tumorigenity in OSCC cells.
Atti del 46th Meeting of the Continental European Division of the International Association for Dental Research (CED-IADR).
Autore/i: Mascitti, M.; Santarelli, Andrea; Pozzi, Valentina; Sartini, Davide; Morganti, Stefano; Giuliante, Rachela; Rocchetti, Romina; Rubini, Corrado; Lo Muzio, L.; Emanuelli, Monica
Classificazione: 4 Contributo in Atti di Convegno (Proceeding)
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/208917

Trail over expression inversely correlates with histological differentiation in intestinal-type sinonasal adenocarcinoma.
INTERNATIONAL JOURNAL OF SURGICAL ONCOLOGY
Autore/i: Re, Massimo; Santarelli, Andrea; Mascitti, M.; Bambini, Fabrizio; Lo Muzio, L.; Zizzi, Antonio; Rubini, Corrado
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/123063

Is expression of p120ctn in oral squamous cell carcinomas a prognostic factor?
ORAL SURGERY, ORAL MEDICINE, ORAL PATHOLOGY AND ORAL RADIOLOGY
Autore/i: Lo Muzio, L.; Pannone, G.; Santarelli, Andrea; Bambini, Fabrizio; Mascitti, M.; Rubini, Corrado; Testa, N. F.; Dioguardi, M.; Leuci, S.; Bascones, A.; Reynolds, A. B.; Mariggiò, M. A.
Classificazione: 1 Contributo su Rivista
Abstract: OBJECTIVES: p120ctn is a component of the catenin family. To date, there have only been two studies examining expression levels of p120ctn in oral squamous cell carcinoma (OSCC). MATERIALS AND METHODS: Paraffined specimens of 113 OSCCs and 12 of normal mucosa were examined by immunohistochemistry. Frozen samples of 20 OSCCs and 5 of normal mucosa were examined by Western blot (WB). Results were correlated with clinicopathological parameters. Five cell lines were examined by immunofluorescence, immunocytochemistry, and WB to show immunoreactivity and cellular localization of p120ctn. RESULTS: Altered p120ctn expression was observed in 109/113 cases of OSCC. Heterogenous cytoplasmic/nuclear expression was associated with loss of membranous distribution (88/113 cases). Complete loss of expression was noted in 21/113 cases. Increased cytoplasmic expression was evident in all positive cases, without significant correlation among p120ctn staining/pattern and grading/stage. Reduction/absence of p120ctn expression was related to poor prognosis (P < .05). CONCLUSION: p120ctn delocalization/loss of expression could be an independent prognostic marker in OSCC.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/107864

Validation of diagnostic panel for OSCC
Atti del 19° Congresso Nazionale dei Docenti di Discipline Odontostomatologiche
Autore/i: Mascitti, M.; Santarelli, Andrea; Quaranta, Alessandro; Mastrovincenzo, M.; Rubini, Corrado; Lo Muzio, L.; Procaccini, Maurizio
Classificazione: 4 Contributo in Atti di Convegno (Proceeding)
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/82461

CSCs selection, characterization and tumorigenic potential in OSCC.
ORAL DISEASES
Autore/i: Mascitti, M.; Santarelli, Andrea; Sartini, Davide; Rocchetti, R.; Pozzi, Valentina; Morganti, Stefano; Rubini, Corrado; Zizzi, A.; Emanuelli, Monica; Lo Muzio, L.
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/80721