198 pubblicazioni classificate nel seguente modo:

Contribution of the Paraoxonase-2 Enzyme to Cancer Cell Metabolism and Phenotypes
BIOMOLECULES
Autore/i: Campagna, R; Serritelli, En; Salvolini, E; Schiavoni, V; Cecati, M; Sartini, D; Pozzi, V; Emanuelli, M
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/327434

Knockdown of nicotinamide N-methyltransferase suppresses proliferation, migration and chemoresistance of Merkel cell carcinoma cells in vitro
HUMAN CELL
Autore/i: Pozzi, V; Molinelli, E; Campagna, R; Serritelli, En; Cecati, M; De Simoni, E; Sartini, D; Goteri, G; Martin, Ni; van Haren, Mj; Salvolini, E; Simonetti, O; Offidani, A; Emanuelli, M
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/327436

Targeting nicotinamide N-methyltransferase decreased aggressiveness of osteosarcoma cells
EUROPEAN JOURNAL OF CLINICAL INVESTIGATION
Autore/i: Serritelli, En; Sartini, D; Campagna, R; Pozzi, V; Martin, Ni; van Haren, Mj; Salvolini, E; Cecati, M; Rubini, C; Emanuelli, M
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/327435

Involvment of the enzyme nicotinamide N-methyltransferase in proliferation, invasive capacity and chemosensitivity of human osteosarcoma.
34a Riunione Nazionale “A. Castellani” dei Dottorandi di Ricerca in Discipline Biochimiche, 5-9 Giugno 2023, Brallo di Pregola (PV).
Autore/i: Serritelli, En; Pozzi, V; Campagna, R; Sartini, D; Salvolini, E; Rubini, C; Emanuelli, M
Classificazione: 4 Contributo in Atti di Convegno (Proceeding)
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/318951

Exploring the role of paraoxonase-2 in human clear cell renal cell carcinoma: in vitro effect of shRNA-mediated gene silencing on cell proliferation and chemosensitivity
62° Congresso della Società Italiana di Biochimica e Biologia Molecolare (SIB), Firenze, 7-9 Settembre, 2023
Autore/i: Campagna, R; Schiavoni, V; Pozzi, V; Sartini, D; Milanese, G; Galosi, Ab; Emanuelli, M; Salvolini, E
Classificazione: 4 Contributo in Atti di Convegno (Proceeding)
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/324074

Nicotinamide N-methyltransferase: a key enzyme of pyridine metabolism and a target for human osteosarcoma treatment
“Deciphering and Reshaping Nucleic Acids” 1st Workshop of the SIB group “Nucleic Acids”, October 5th and 6th, 2023, Aula Circolare, University of Calabria, Rende (CS).
Autore/i: Campagna, R; Serritelli, E. N.; Pozzi, V; Sartini, D; Salvolini, E; Rubini, C; Goteri, G; Emanuelli, M
Classificazione: 4 Contributo in Atti di Convegno (Proceeding)
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/324072

Enhanced expression of nicotinamide N-methyltransferase in human osteosarcoma: potential role in tumor growth and chemoresistance
62° Congresso della Società Italiana di Biochimica e Biologia Molecolare (SIB), Firenze, 7-9 Settembre, 2023.
Autore/i: Serritelli, En; Pozzi, V; Campagna, R; Sartini, D; Salvolini, E; Rubini, C; Goteri, G; Emanuelli, M
Classificazione: 4 Contributo in Atti di Convegno (Proceeding)
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/324073

Nicotinamide N-methyltransferase, a promising marker for human Merkel cell carcinoma
Understanding cancer metabolism: exploring tumor heterogeneity to advance cancer therapy. 3rd BDT Workshop, Sezione Biochimica dei Tumori, Società Italina di Biochimica e Biologia Molecolare (SIB), Catanzaro, 29-30 Giugno, 2023.
Autore/i: Pozzi, V; Molinelli, E; Campagna, R; Serritelli, En; Cecati, M; Sartini, D; Goteri, G; Salvolini, E; Simonetti, O; Offidani, A; Emanuelli, M
Classificazione: 4 Contributo in Atti di Convegno (Proceeding)
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/318212

Paraoxonase-2 expression in oral squamous cell carcinoma
HUMAN CELL
Autore/i: Campagna, R; Pozzi, V; Salvucci, A; Togni, L; Mascitti, M; Sartini, D; Salvolini, E; Santarelli, A; Lo Muzio, L; Emanuelli, M
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/313648

Recent Advances in the Management of Clear Cell Renal Cell Carcinoma: Novel Biomarkers and Targeted Therapies
CANCERS
Autore/i: Schiavoni, V; Campagna, R; Pozzi, V; Cecati, M; Milanese, G; Sartini, D; Salvolini, E; Galosi, Ab; Emanuelli, M
Classificazione: 1 Contributo su Rivista
Abstract: Renal cell carcinoma (RCC) belongs to a heterogenous cancer group arising from renal tubular epithelial cells. Among RCC subtypes, clear cell renal cell carcinoma (ccRCC) is the most common variant, characterized by high aggressiveness, invasiveness and metastatic potential, features that lead to poor prognosis and high mortality rate. In addition, diagnosis of kidney cancer is incidental in the majority of cases, and this results in a late diagnosis, when the stage of the disease is advanced and the tumor has already metastasized. Furthermore, ccRCC treatment is complicated by its strong resistance to chemo- and radiotherapy. Therefore, there is active ongoing research focused on identifying novel biomarkers which could be useful for assessing a better prognosis, as well as new molecules which could be used for targeted therapy. In this light, several novel targeted therapies have been shown to be effective in prolonging the overall survival of ccRCC patients. Thus, the aim of this review is to analyze the actual state-of-the-art on ccRCC diagnosis, prognosis and therapeutic options, while also reporting the recent advances in novel biomarker discoveries, which could be exploited for a better prognosis or for targeted therapy.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/318211

Enzymes Dysregulation in Cancer: From Diagnosis to Therapeutical Approaches
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Autore/i: Pozzi, V; Campagna, R; Sartini, D; Emanuelli, M
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/324071

The Effect and the Potential Use of Magnetic–Dam Barrier in Guided Bone Regeneration: A Laboratory Study
APPLIED SCIENCES
Autore/i: Memè, L; Bambini, F; Gallusi, G; Sartini, D; Pozzi, V; Emanuelli, M; Strappa, Em; Mummolo, S
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/313649

Role Played by Paraoxonase-2 Enzyme in Cell Viability, Proliferation and Sensitivity to Chemotherapy of Oral Squamous Cell Carcinoma Cell Lines
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Autore/i: Campagna, R; Belloni, A; Pozzi, V; Salvucci, A; Notarstefano, V; Togni, L; Mascitti, M; Sartini, D; Giorgini, E; Salvolini, E; Santarelli, A; Lo Muzio, L; Emanuelli, M
Classificazione: 1 Contributo su Rivista
Abstract: Oral squamous cell carcinoma represents the most aggressive and frequent form of head and neck cancer. Due to drug resistance, the 5-year survival rate of patients with advanced disease is less than 50%. In order to identify molecular targets for effective oral cancer treatment, we focused on paraoxonase-2 enzyme. Indeed, based on data previously obtained from preliminary immunohistochemistry and Western blot analyses performed on tissue specimens, the enzyme was found to be upregulated in tumor compared with normal oral mucosa. Therefore, paraoxonase-2 gene silencing was achieved in HSC-3 and HOC621 oral cancer cell lines, and the effect on cell proliferation, viability, apoptosis induction and sensitivity to cisplatin and 5-fluorouracil treatment was evaluated. Fourier Transform InfraRed Microspectroscopy analyzed alterations of cellular macromolecules upon treatment. Enzyme level and cell proliferation were also determined in cisplatin-resistant clones obtained from HOC621 cell line, as well as in parental cells. Reported data showed that paraoxonase-2 knockdown led to a reduction of cell proliferation and viability, as well as to an enhancement of sensitivity to cisplatin, together with the activation of apoptosis pathway. Spectroscopical data demonstrated that, under treatment with cisplatin, oxidative damage exerted on lipids and proteins was markedly more evident in cells down-regulating paraoxonase-2 compared to controls. Interestingly, enzyme expression, as well as cell proliferation were significantly higher in cisplatin-resistant compared with control HOC621 cells. Taken together these results seem to candidate the enzyme as a promising target for molecular treatment of this neoplasm.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/310087

Paraoxonase-2 is upregulated in triple negative breast cancer and contributes to tumor progression and chemoresistance
HUMAN CELL
Autore/i: Campagna, R; Pozzi, V; Giorgini, S; Morichetti, D; Goteri, G; Sartini, D; Serritelli, En; Emanuelli, M
Classificazione: 1 Contributo su Rivista
Abstract: Triple negative breast cancer (TNBC) displays a high aggressive behavior, tendency to relapse and early metastasize, leading to poor prognosis. The lack of estrogen receptors, and human epidermal growth factor receptor 2, prevents the use of endocrine or molecular targeted therapy, being therapeutical options for TNBC managements mostly limited to surgery, radiotherapy and mainly chemotherapy. While an important number of TNBCs initially responds to chemotherapy, they are prone to develop chemoresistance over the time. Thus, there is an urgent need to identify novel molecular targets to improve the outcome of chemotherapy in TNBC. In this work we focused on the enzyme paraoxonase-2 (PON2) which has been reported to be overexpressed in several tumors contributing to cancer aggressiveness and chemoresistance. Through a case– control study, we analyzed PON2 immunohistochemical expression in breast cancer molecular subtypes Luminal A, Luminal B, Luminal B HER2+, HER2 + and TNBC. Subsequently, we evaluated the in vitro effect of PON2 downregulation on cell proliferation and response to chemotherapeutics. Our results showed that the PON2 expression levels were significantly upregulated in the infiltrating tumors related to the subtypes Luminal A, HER2+ and TNBC compared to the healthy tissue. Furthermore, PON2 downregulation led to a decrease in cell proliferation of breast cancer cells, and significantly enhanced the cytotoxicity of chemotherapeutics on the TNBC cells. Although further analyses are necessary to deeply understand the mechanisms by which the enzyme could participate to breast cancer tumorigenesis, our results seem to demonstrate that PON2 could represent a promising molecular target for TNBC treatment.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/313647

Immunohistochemical expression of nicotinamide N-methyltransferase in lymph node metastases from cutaneous malignant melanoma
HUMAN CELL
Autore/i: Sartini, Davide; Molinelli, Elisa; Pozzi, Valentina; Campagna, Roberto; Salvolini, Eleonora; Rubini, Corrado; Goteri, Gaia; Simonetti, Oriana; Campanati, Anna; Offidani, Annamaria; Emanuelli, Monica
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/306463

Nicotinamide N-methyltransferase, a promising marker for human osteosarcoma
BIOCHIMICA CLINICA
Autore/i: Pozzi, V; Serritelli, En; Campagna, R; Sartini, D; Salvolini, E; Rubini, C; Emanuelli, M
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/307321

Paraoxonase-2: a novel molecular target to enhance chemosensitivity in melanoma and triple negative breast cancer cells
2nd DiSVA-MaSBiC Annual Symposium, 13-14 Ottobre2022, Ancona
Autore/i: Campagna, R; Bacchetti, T; Salvolini, E; Pozzi, V; Serritelli, E; Giorgini, S; Molinelli, E; Simonetti, O; Sartini, D; Campanati, A; Ferretti, G; Goteri, G; Offidani, A; Emanuelli, M
Classificazione: 4 Contributo in Atti di Convegno (Proceeding)
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/307322

FTIRM: an analytical approach to test the effectiveness of cisplatin treatment on PON2 silenced otscc cells
XLVIII Congresso Nazionale di Chimica Inorganica, Pisa, 6-9 Settembre 2022
Autore/i: Belloni, A; Notarstefano, V; Campagna, R; Pozzi, V; Sartini, D; Giorgini, E; Emanuelli, M
Classificazione: 4 Contributo in Atti di Convegno (Proceeding)
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/307241

Role of paraoxonase-2 in triple-negative breast cancer: effect of shRNA-mediated gene silencing on cell proliferation and chemosensitivity
16° Congresso Nazionale della Federazione Italiana Scienze della Vita (FISV), Reggia di Portici (Napoli), 14-16 Settembre 2022
Autore/i: Campagna, R; Giorgini, S; Morichetti, D; Sartini, D; Pozzi, V; Goteri, G; Emanuelli, M
Classificazione: 4 Contributo in Atti di Convegno (Proceeding)
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/307224

Paraoxonase-2 (PON2) is upregulated in triple negative breast cancer and contributes to the tumor progression and chemoresistance
Congresso della Sezione Tosco-Umbro-Marchigiana (TUM) sella Società Italiana di Biochimica e Biologia Molecolare (SIB), 1 Dicembre 2022, Perugia
Autore/i: Campagna, R; Giorgini, S; Morichetti, D; Sartini, D; Pozzi, V; Goteri, G; Emanuelli, M.
Classificazione: 4 Contributo in Atti di Convegno (Proceeding)
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/310089

Role of nicotinamide N-methyltransferase in human osteosarcoma: in vitro effect of shRNA-mediated gene silencing on tumorigenicity
Congresso della Sezione Tosco-Umbro-Marchigiana (TUM) sella Società Italiana di Biochimica e Biologia Molecolare (SIB), 1 Dicembre 2022, Perugia
Autore/i: Serritelli, En; Pozzi, V; Campagna, R; Sartini, D; Salvolini, E; Rubini, C; Emanuelli, M.
Classificazione: 4 Contributo in Atti di Convegno (Proceeding)
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/310088

Epithelial Biological Response to Machined Titanium vs. PVD Zirconium-Coated Titanium: An In Vitro Study
MATERIALS
Autore/i: Meme', Lucia; Sartini, Davide; Pozzi, Valentina; Emanuelli, Monica; Strappa, Enrico M; Bittarello, Paolo; Bambini, Fabrizio; Gallusi, Gianni
Classificazione: 1 Contributo su Rivista
Abstract: The aim of this study was to compare the epithelial biological response to machined titanium Ti-6Al-4V grade 5 and titanium Ti-6Al-4V grade 5 coated with zirconia (ZrN) by physical vapor deposition (PVD). Human keratinocytes were cultured in six-well plates. Machined titanium TiAl4V4 grade 5 (T1) and ZrN-coated titanium TiAl4V4 grade 5 (T2) discs were placed in two different wells. The remaining two wells served as control (C). Scanning electron microscopy (SEM) and energy-dispersive spectroscopy (EDS) were performed to compare the T1 and T2 surfaces. Subsequent analyses were performed to explore the effect of T1 and T2 contact with human keratinocyte HUKE cell lines. Cell viability was evaluated using a trypan blue exclusion test and MTT assay. Cell lysates from C, T1, and T2 were Western blotted to evaluate E-cadherin and Integrin-alpha 6 beta 4 expression. SEM revealed that T2 was smoother and more homogeneous than T1. EDS showed homogeneous and uniform distribution of ZrN coating on T2. Cell viability analyses did not show significant differences between T1 and T2. Furthermore, E-cadherin and Integrin-alpha 6 beta 4 expressions of the epithelial cells cultured in T1 and T2 were similar. Therefore, titanium Ti-6Al-4V grade 5 surfaces coated with ZrN by PVD seem to be similar substrates to the uncoated surfaces for keratinocyte adhesion and proliferation.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/308643

The Double-Edged Sword of Oxidative Stress in Skin Damage and Melanoma: From Physiopathology to Therapeutical Approaches
ANTIOXIDANTS
Autore/i: Emanuelli, M; Sartini, D; Molinelli, E; Campagna, R; Pozzi, V; Salvolini, E; Simonetti, O; Campanati, A; Offidani, A
Classificazione: 1 Contributo su Rivista
Abstract: The skin is constantly exposed to exogenous and endogenous sources of reactive oxygen species (ROS). An adequate balance between ROS levels and antioxidant defenses is necessary for the optimal cell and tissue functions, especially for the skin, since it must face additional ROS sources that do not affect other tissues, including UV radiation. Melanocytes are more exposed to oxidative stress than other cells, also due to the melanin production process, which itself contributes to generating ROS. There is an increasing amount of evidence that oxidative stress may play a role in many skin diseases, including melanoma, being the primary cause or being a cofactor that aggravates the primary condition. Indeed, oxidative stress is emerging as another major force involved in all the phases of melanoma development, not only in the arising of the malignancy but also in the progression toward the metastatic phenotype. Furthermore, oxidative stress seems to play a role also in chemoresistance and thus has become a target for therapy. In this review, we discuss the existing knowledge on oxidative stress in the skin, examining sources and defenses, giving particular consideration to melanocytes. Therefore, we focus on the significance of oxidative stress in melanoma, thus analyzing the possibility to exploit the induction of oxidative stress as a therapeutic strategy to improve the effectiveness of therapeutic management of melanoma.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/298762

Effects of Fermented Wheat Germ Extract on Oral Cancer Cells: An In Vitro Study
NUTRITION AND CANCER
Autore/i: Zhurakivska, K; Risteli, M; Salo, T; Sartini, D; Salvucci, A; Troiano, G; Lo Muzio, L; Emanuelli, M.
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/292072

Nicotinamide N-Methyltransferase as Promising Tool for Management of Gastrointestinal Neoplasms
BIOMOLECULES
Autore/i: Pozzi, V; Campagna, R; Sartini, D; Emanuelli, M
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/307221

C. spinosa L. subsp. rupestris Phytochemical Profile and Effect on Oxidative Stress in Normal and Cancer Cells
MOLECULES
Autore/i: Bacchetti, T; Campagna, R; Sartini, D; Cecati, M; Morresi, C; Bellachioma, L; Martinelli, E; Rocchetti, G; Lucini, L; Ferretti, G; Emanuelli, M
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/307222

PON2 silencing to evaluate the effectiveness of Cisplatin treatment in OSCC cells by FTIRM analyses
Merck Young Symposium 2021, Rimini (IT), 22-24 November, 2021.
Autore/i: Belloni, A; Notarstefano, A; Salvucci, A; Campagna, R; Spinelli, G; Sartini, D; Giorgini, E; Emanuelli, M
Classificazione: 4 Contributo in Atti di Convegno (Proceeding)
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/301279

Immunohistochemical analysis of paraoxonase-2 in actinic keratosis and squamous cell carcinoma
61° Congresso Nazionale della Società Italiana di Biochimica e Biologia Molecolare (SIB), Virtual Edition, 23-24 Settembre 2021
Autore/i: Campagna, R; Sartini, D; Lucarini, G; Salvolini, E; Molinelli, E; Brisigotti, V; Campanati, A; Bacchetti, T; Ferretti, G; Offidani, A; Emanuelli, M
Classificazione: 4 Contributo in Atti di Convegno (Proceeding)
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/291905

Potent Inhibition of Nicotinamide N-Methyltransferase by Alkene-Linked Bisubstrate Mimics Bearing Electron Deficient Aromatics
JOURNAL OF MEDICINAL CHEMISTRY
Autore/i: Gao, Y; van Haren, Mj; Buijs, N; Innocenti, P; Zhang, Y; Sartini, D; Campagna, R; Emanuelli, M; Parsons, Rb; Jespers, W; Gutiérrez-de-Terán, H; van Westen, Gjp; Martin, Ni
Classificazione: 1 Contributo su Rivista
Abstract: Nicotinamide N-methyltransferase (NNMT) methylates nicotinamide (vitamin B3) to generate 1-methylnicotinamide (MNA). NNMT overexpression has been linked to a variety of diseases, most prominently human cancers, indicating its potential as a therapeutic target. The development of small-molecule NNMT inhibitors has gained interest in recent years, with the most potent inhibitors sharing structural features based on elements of the nicotinamide substrate and the S-adenosyl-L-methionine (SAM) cofactor. We here report the development of new bisubstrate inhibitors that include electron-deficient aromatic groups to mimic the nicotinamide moiety. In addition, a trans-alkene linker was found to be optimal for connecting the substrate and cofactor mimics in these inhibitors. The most potent NNMT inhibitor identified exhibits an IC50 value of 3.7 nM, placing it among the most active NNMT inhibitors reported to date. Complementary analytical techniques, modeling studies, and cell-based assays provide insights into the binding mode, affinity, and selectivity of these inhibitors.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/291901

Expression of Paraoxonase-2 in Different Stages of Cutaneous T Cell Lymphoma: Is It a Potential Biomarker for Aggressiveness?
BLOOD
Autore/i: Morsia, E; Rupoli, S; Molinelli, E; Sartini, D; Offidani, A; Emanuelli, M; Salvolini, E; Olivieri, A; Goteri, G
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/295149

Esterase-Sensitive Prodrugs of a Potent Bisubstrate Inhibitor of Nicotinamide N-Methyltransferase (NNMT) Display Cellular Activity
BIOMOLECULES
Autore/i: Van Haren, Mj; Gao, Y; Buijs, N; Campagna, R; Sartini, D; Emanuelli, M; Mateuszuk, L; Kij, A; Chlopicki, S; Escudé Martinez de Castilla, P; Schiffelers, R; Martin, Ni
Classificazione: 1 Contributo su Rivista
Abstract: A recently discovered bisubstrate inhibitor of Nicotinamide N-methyltransferase (NNMT) was found to be highly potent in biochemical assays with a single digit nanomolar IC50 value but lacking in cellular activity. We, here, report a prodrug strategy designed to translate the observed potent biochemical inhibitory activity of this inhibitor into strong cellular activity. This prodrug strategy relies on the temporary protection of the amine and carboxylic acid moieties of the highly polar amino acid side chain present in the bisubstrate inhibitor. The modification of the carboxylic acid into a range of esters in the absence or presence of a trimethyl-lock (TML) amine protecting group yielded a range of candidate prodrugs. Based on the stability in an aqueous buffer, and the confirmed esterase-dependent conversion to the parent compound, the isopropyl ester was selected as the preferred acid prodrug. The isopropyl ester and isopropyl ester-TML prodrugs exhibit improved cell permeability, which also translates to significantly enhanced cellular activity as established using assays designed to measure the enzymatic activity of NNMT in live cells.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/292073

Paraoxonase-2: A potential biomarker for skin cancer aggressiveness
EUROPEAN JOURNAL OF CLINICAL INVESTIGATION
Autore/i: Bacchetti, T; Salvolini, E; Pompei, V; Campagna, R; Molinelli, E; Brisigotti, V; Togni, L; Lucarini, G; Sartini, D; Campanati, A; Mattioli-Belmonte, M; Rubini, C; Ferretti, G; Offidani, A; Emanuelli, M
Classificazione: 1 Contributo su Rivista
Abstract: Background: Cutaneous neoplasms include melanoma and non-melanoma skin cancers (NMSCs). Among NMSCs, basal cell carcinoma (BCC) represents the most common lesion. On the contrary, although accounting for less than 5% of all skin cancers, melanoma is responsible for most of cutaneous malignancy-related deaths. Paraoxonase-2 (PON2) is an intracellular enzyme exerting a protective role against production of reactive oxygen species within mitochondrial respiratory chain. Recently, a growing attention has been focused on exploring the role of PON2 in cancer. The aim of this study was to investigate the diagnostic and prognostic role of PON2 in skin neoplasms. Materials and methods: 36 cases of BCC, distinguished between nodular and infiltrative lesions, as well as 29 melanoma samples were analysed by immunohistochemistry to evaluate PON2 protein expression. Subsequent statistical analyses were carried out to explore the existence of correlations between intratumour enzyme levels and clinicopathological features. Results: Results obtained showed PON2 overexpression in BCCs compared with controls. In particular, distinguishing between less and more aggressive tumour forms, we found no significant differences in enzyme levels between nodular BCCs and controls. Conversely, PON2 expression was significantly higher in infiltrative BCCs compared with controls. Moreover, the enzyme was strongly upregulated in melanoma samples with respect to controls. Interestingly, PON2 levels were positively correlated with Breslow thickness, Clark level, regression, mitoses, lymph node metastases, primary tumour (pT) parameter and pathological stage. Conclusions: Reported findings seem to suggest that PON2 expression levels could be positively related with tumour aggressiveness of both BCC and melanoma.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/285972

Saliva proteomics as fluid signature of inflammatory and immune mediated skin diseases
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Autore/i: Campanati, A; Martina, E; Diotallevi, F; Radi, G; Marani, A; Sartini, D; Emanuelli, M; Kontochristopoulos, G; Rigopoulos, D; Gregoriou, S; Offidani, A
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/291522

Treatment‐related dysgeusia in oral and oropharyngeal cancer: A comprehensive review
NUTRIENTS
Autore/i: Togni, L.; Mascitti, M.; Vignigni, A.; Alia, S.; Sartini, D.; Barlattani, A.; Emanuelli, M.; Santarelli, A.
Classificazione: 1 Contributo su Rivista
Abstract: Oral cancer is the most common tumor of the head and neck region. Its management is based on surgical and systemic therapies. Taste disorders represent the most common side effect of these treatments; indeed, dysgeusia is noted by 70% of oral cancer patients. Despite survival remaining the primary endpoint of cancer patients, taste impairments can cause psychological distress. This comprehensive review describes the last decade’s knowledge from the literature regarding taste alterations in patients with oral and oropharyngeal squamous cell carcinoma. A total of 26 articles in English, including prospective, cross‐sectional, and case–control studies, and clinical trials were evaluated. Literature analysis shows that anti‐cancer treatments can destroy taste cells, decrease and alter their receptors, and interrupt nerve transmission. Furthermore, the tumour itself can destroy the oral mucosal lining, which encloses the taste buds. Dysgeusia typically occurs in 3– 4 weeks of treatments, and usually taste sensation is recovered within 3–12 months. However, some patients exhibit incomplete or no recovery, even several years later. Thus, dysgeusia can become a chronic issue and negatively influence patients’ quality of life, worsening their dysphagia and their nutritional status. Physicians should be focused on preventing oncological treatment‐related symptoms, offering the most suitable personalized support during therapy.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/293485

Differential immunohistochemical expression of paraoxonase-2 in actinic keratosis and squamous cell carcinoma
HUMAN CELL
Autore/i: Sartini, D; Campagna, R; Lucarini, G; Pompei, V; Salvolini, E; Mattioli-Belmonte, M; Molinelli, E; Brisigotti, V; Campanati, A; Bacchetti, T; Ferretti, G; Offidani, A; Emanuelli, M
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/291523

Nicotinamide N-Methyltransferase in Head and Neck Tumors: A Comprehensive Review
BIOMOLECULES
Autore/i: Togni, L; Mascitti, M; Sartini, D; Campagna, R; Pozzi, V; Salvolini, E; Offidani, A; Santarelli, A; Emanuelli, M
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/295330

The Utility of Nicotinamide N-Methyltransferase as a Potential Biomarker to Predict the Oncological Outcomes for Urological Cancers: An Update
BIOMOLECULES
Autore/i: Campagna, R; Pozzi, V; Spinelli, G; Sartini, D; Milanese, G; Galosi, Ab; Emanuelli, M
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/291902

Beyond Nicotinamide Metabolism: Potential Role of Nicotinamide N‐Methyltransferase as a Biomarker in Skin Cancers
CANCERS
Autore/i: Campagna, R; Pozzi, V; Sartini, D; Salvolini, E; Brisigotti, V; Molinelli, E; Campanati, A; Offidani, A; Emanuelli, M
Classificazione: 1 Contributo su Rivista
Abstract: kin cancers (SC) collectively represent the most common type of malignancy in white populations. SC includes two main forms: malignant melanoma and non-melanoma skin cancer (NMSC). NMSC includes different subtypes, namely, basal cell carcinoma (BCC), squamous cell carcinoma (SCC), Merkel cell carcinoma (MCC), and keratoacanthoma (KA), together with the two pre-neoplastic conditions Bowen disease (BD) and actinic keratosis (AK). Both malignant melanoma and NMSC are showing an increasing incidence rate worldwide, thus representing an important challenge for health care systems, also because, with some exceptions, SC are generally characterized by an aggressive behavior and are often diagnosed late. Thus, identifying new biomarkers suitable for diagnosis, as well as for prognosis and targeted therapy is mandatory. Nicotinamide N-methyltransferase (NNMT) is an enzyme that is emerging as a crucial player in the progression of several malignancies, while its substrate, nicotinamide, is known to exert chemopreventive effects. Since there is increasing evidence regarding the involvement of this enzyme in the malignant behavior of SC, the current review aims to summarize the state of the art as concerns NNMT role in SC and to support future studies focused on exploring the diagnostic and prognostic potential of NNMT in skin malignancies and its suitability for targeted therapy.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/295329

Nicotinamide N-methyltransferase in endothelium protects against oxidant stress-induced endothelial injury
BIOCHIMICA ET BIOPHYSICA ACTA. MOLECULAR CELL RESEARCH
Autore/i: Campagna, R; Mateuszuk, Ł; Wojnar-Lason, K; Kaczara, P; Tworzydło, A; Kij, A; Bujok, R; Mlynarski, J; Wang, Y; Sartini, D; Emanuelli, M; Chlopicki, S
Classificazione: 1 Contributo su Rivista
Abstract: Nicotinamide N-methyltransferase (NNMT, EC 2.1.1.1.) plays an important role in the growth of many different tumours and is also involved in various non-neoplastic disorders. However, the presence and role of NNMT in the endothelium has yet to be specifically explored. Here, we characterized the functional activity of NNMT in the endothelium and tested whether NNMT regulates endothelial cell viability. NNMT in endothelial cells (HAEC, HMEC-1 and EA.hy926) was inhibited using two approaches: pharmacological inhibition of the enzyme by NNMT inhibitors (5-amino-1-methylquinoline – 5MQ and 6-methoxynicotinamide – JBSF-88) or by shRNAmediated silencing. Functional inhibition of NNMT was confirmed by LC/MS/MS-based analysis of impaired MNA production. The effects of NNMT inhibition on cellular viability were analyzed in both the absence and presence of menadione. Our results revealed that all studied endothelial lines express relatively high levels of functionally active NNMT compared with cancer cells (MDA-MB-231). Although the aldehyde oxidase 1 enzyme was also expressed in the endothelium, the further metabolites of N1-methylnicotinamide (N1-methyl-2-pyridone-5-carboxamide and N1-methyl-4-pyridone-3-carboxamide) generated by this enzyme were not detected, suggesting that endothelial NNMT-derived MNA was not subsequently metabolized in the endothelium by aldehyde oxidase 1. Menadione induced a concentration-dependent decrease in endothelial viability as evidenced by a decrease in cell number that was associated with the upregulation of NNMT and SIRT1 expression in the nucleus in viable cells. The suppression of the NNMT activity either by NNMT inhibitors or shRNA-based silencing significantly decreased the endothelial cell viability in response to menadione. Furthermore, NNMT inhibition resulted in nuclear SIRT1 expression downregulation and upregulation of the phosphorylated form of SIRT1 on Ser47. In conclusion, our results suggest that the endothelial nuclear NNMT/SIRT1 pathway exerts a cytoprotective role that safeguards endothelial cell viability under oxidant stress insult.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/290823

Nicotinamide N-methyltransferase gene silencing enhances chemosensitivity of melanoma cell lines
PIGMENT CELL & MELANOMA RESEARCH
Autore/i: Campagna, R; Salvolini, E; Pompei, V; Pozzi, V; Salvucci, A; Molinelli, E; Brisigotti, V; Sartini, D; Campanati, A; Offidani, A; Emanuelli, M
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/290822

Macrocyclic peptides as allosteric inhibitors of nicotinamide N-methyltransferase (NNMT)
RSC CHEMICAL BIOLOGY
Autore/i: Van Haren, Mj; Zhang, Y; Thijssen, V; Buijs, N; Gao, Y; Mateuszuk, L; Fedak, Fa; Kij, A; Campagna, R; Sartini, D; Emanuelli, M; Chlopicki, S; Jongkees, Sak; Martin, Ni
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/291903

Effects of fermented wheat germ extract on the oral squamous cell carcinoma. An in vitro study
VIII International Symposium Advances in oral Cancer – Online, 24-25 Settembre 2020
Autore/i: Zhurakivska, K; Troiano, G; Risteli, M; Salo, T; Sartini, D; Salvucci, A; Lo Muzio, L; Emanuelli, M
Classificazione: 4 Contributo in Atti di Convegno (Proceeding)
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/284957

Bladder Cancer Chemosensitivity is Affected by Paraoxonase-2 Expression
ANTIOXIDANTS
Autore/i: Fumarola, S; Cecati, M; Sartini, D; Ferretti, G; Milanese, G; Galosi, Ab; Pozzi, V; Campagna, R; Morresi, C; Emanuelli, M; Bacchetti, T.
Classificazione: 1 Contributo su Rivista
Abstract: The goal of the current study was to identify potential roles of paraoxonase-2 in bladder carcinogenesis. T24 bladder cancer cells were transfected with plasmids inducing paraoxonase-2 silencing or overexpression. Upon the selection of clones stably down-or upregulating paraoxonase-2, cell proliferation, migration, and the production of reactive oxygen species were evaluated, before and after treatment with cisplatin and gemcitabine, used alone or in combination. The activity levels of both caspase-3 and caspase-8 were also analyzed. shRNA-mediated gene silencing and the overexpression of paraoxonase-2 revealed that the enzyme was able to promote both the proliferation and migration of T24 cells. Moreover, the knockdown of paraoxonase-2 was significantly associated with a reduced cell viability of T24 cells treated with chemotherapeutic drugs and led to both an increase of reactive oxygen species production and caspase-3 and caspase-8 activation. Conversely, under treatment with anti-neoplastic compounds, a higher proliferative capacity was found in T24 cells overexpressing paraoxonase-2 compared with controls. In addition, upon enzyme upregulation, both the production of reactive oxygen species and activation of caspase-3 and caspase-8 were reduced. Although further analyses will be required to fully understand the involvement of paraoxonase-2 in bladder tumorigenesis and in mechanisms leading to the development of chemoresistance, the data reported in this study seem to demonstrate that the enzyme could exert a great impact on tumor progression and susceptibility to chemotherapy, thus suggesting paraoxonase-2 as a novel and interesting molecular target for effective bladder cancer treatment.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/278198

Paraoxonase-2 Silencing Enhances Sensitivity of A375 Melanoma Cells to Treatment with Cisplatin
ANTIOXIDANTS
Autore/i: Campagna, R; Bacchetti, T; Salvolini, E; Pozzi, V; Molinelli, E; Brisigotti, V; Sartini, D; Campanati, A; Ferretti, G; Offidani, A; Emanuelli, M.
Classificazione: 1 Contributo su Rivista
Abstract: Melanoma represents the most aggressive skin cancer, being responsible for the majority of deaths related with these neoplasms. Despite chemotherapy represents a frontline approach for management of the advanced stages of the disease, it displayed poor response rates and short-term efficacy due to melanoma cell resistance. Therefore, the discovery of molecules that can be used for effective targeted therapy of melanoma is crucial. In this study, we evaluated the impact of paraoxonase-2 (PON2) silencing on proliferation, viability, and resistance to treatment of the A375 melanoma cell line with chemotherapeutic drugs dacarbazine (DTIC) and cisplatin (CDDP). Due to the enzymes ability to counteract oxidative stress, we also evaluated the effect of enzyme knockdown on reactive oxygen species (ROS) production in cells treated with CDDP. The data reported clearly demonstrated that PON2 knockdown led to a significant reduction of cell proliferation and viability, as well as to an enhancement of A375 sensitivity to CDDP treatment. Moreover, enzyme downregulation was associated with an increase of ROS production in CDDP-treated cells. Although further analyses will be necessary to understand how PON2 could influence melanoma cell metabolism and phenotype, our results seem to suggest that the enzyme may serve as an interesting molecular target for effective melanoma treatment.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/285973

Differential expression of nicotinamide N-methyltransferase in primary and recurrent ameloblastomas and odontogenic keratocysts
EUROPEAN JOURNAL OF CLINICAL INVESTIGATION
Autore/i: Mascitti, M; Sartini, D; Togni, L; Pozzi, V; Rubini, C; Santarelli, A; Emanuelli, M
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/278206

Cancer stem cell enrichment is associated with enhancement of nicotinamide N-methyltransferase expression
IUBMB LIFE
Autore/i: Pozzi, V; Salvolini, E; Lucarini, G; Salvucci, A; Campagna, R; Rubini, C; Sartini, D; Emanuelli, M
Classificazione: 1 Contributo su Rivista
Abstract: The cancer stem cell theory states that a subset of tumor cells, termed cancer stem cells (CSCs), has the ability to self-renew and differentiate within the tumors. According to this theory, CSCs would be mainly responsible for tumor initiation, progression, resistance to therapy, recurrence, and metastasis. In this study, a culture system was setup to enrich CSCs from bladder cancer (T24), lung cancer (A549), colorectal cancer (CaCo-2), and osteosarcoma (MG63) cell lines, through sphere formation. Magnetic-activated cell sorting was also used to further increase CSC enrichment. Subsequently, molecular characterization of CSC-enriched cell populations and parental cells was carried out, by exploring the expression levels of stem markers and the enzyme nicotinamide N-methyltransferase (NNMT). Results obtained showed a significant upregulation of stem cell markers in CSC-enriched populations, obtained upon sphere formation, compared with parental counterparts. Moreover, NNMT expression levels were markedly increased in samples enriched with CSCs with respect to control cells. Considering the fundamental role played by CSCs in carcinogenesis, reported data strengthen the hypothesis that sustains a pivotal role of NNMT in cancer growth and metastasis. In addition, these findings could represent an important achievement for the development of new and effective anticancer therapies, based on CSC-associated targets.
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/278211

Differential expression of nicotinamide n-methyltransferase in cutaneous keratoacanthoma and squamous cell carcinoma: an immunohistochemical study
JOURNAL OF THE EUROPEAN ACADEMY OF DERMATOLOGY AND VENEREOLOGY
Autore/i: Sartini, D; Pompei, V; Lucarini, G; Rubini, C; Molinelli, E; Brisigotti, V; Salvolini, E; Campanati, A; Offidani, A; Emanuelli, M
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/272909

Immunohistochemical expression of nicotinamide N-methyltransferase in cutaneous basal and squamous cell carcinomas
60° Congresso Nazionale della Società Italiana di Biochimica e Biologia Molecolare (SIB), Lecce, 18-20 Settembre 2019
Autore/i: Pompei, V; Salvolini, E; Rubini, C; Lucarini, G; Molinelli, E; Brisigotti, V; Sartini, D; Campanati, A; Offidani, A; Emanuelli, M
Classificazione: 4 Contributo in Atti di Convegno (Proceeding)
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/270404

New markers for odontogenic lesions: role of the Nicotinamide N-methyltransferase
FRONTIERS IN PHYSIOLOGY
Autore/i: Luconi, E; Mascitti, M; Giannatempo, G; Lo Muzio, L; Sartini, D; Emanuelli, M
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/272912

Nicotinamide N-methyltransferase inhibition in endothelium: effect on the cellular phenotype, viability and bio-energetics
44th FEBS Congress, Krakow, 6-11 Luglio 2019
Autore/i: Campagna, R; Mateuszuk, L; Kus, K; Sartini, D; Fumarola, S; Emanuelli, M; Chlopicki, S
Classificazione: 4 Contributo in Atti di Convegno (Proceeding)
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/268869

Nicotinamide N-methyltransferase inhibition in endothelial cell lines: effect on the cellular phenotype, cell viability and bioenergetic balance
60° Congresso Nazionale della Società Italiana di Biochimica e Biologia Molecolare (SIB), Lecce, 18-20 Settembre 2019
Autore/i: Campagna, R; Mateuszuk, L; Kus, K; Sartini, D; Fumarola, S; Chlopicki, S; Emanuelli, M
Classificazione: 4 Contributo in Atti di Convegno (Proceeding)
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/270403

Nicotinamide N-methyltransferase in nonmelanoma skin cancers
EUROPEAN JOURNAL OF CLINICAL INVESTIGATION
Autore/i: Pompei, V; Salvolini, E; Rubini, C; Lucarini, G; Molinelli, E; Brisigotti, V; Pozzi, V; Sartini, D; Campanati, A; Offidani, A; Emanuelli, M
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/270386

Effect of High Glucose-Induced Oxidative Stress on Paraoxonase 2 Expression and Activity in Caco-2 Cells
CELLS
Autore/i: Morresi, C; Cianfruglia, L; Sartini, D; Cecati, M; Fumarola, S; Emanuelli, M; Armeni, T; Ferretti, G; Bacchetti, T
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/272911

Analysis of nicotinamide N-methyltransferase in oral malignant melanoma and potential prognostic significance
MELANOMA RESEARCH
Autore/i: Mascitti, M; Santarelli, A; Sartini, D; Rubini, C; Colella, G; Salvolini, E; Ganzetti, G; Offidani, A; Emanuelli, M
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/263183

Expression of nicotinamide N-methyltransferase in non-melanoma skin cancers
FEBS OPENBIO
Autore/i: Pompei, V; Sartini, D; Brisigotti, V; Rubini, C; Offidani, A; Emanuelli, M; Salvolini, E
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/268871

Nitric oxide synthase and VEGF expression in full-term placentas of obese women
HISTOCHEMISTRY AND CELL BIOLOGY
Autore/i: Salvolini, E; Vignini, A; Sabbatinelli, J; Lucarini, G; Pompei, V; Sartini, D; Cester, Am; Ciavattini, A; Mazzanti, L; Emanuelli, Monica
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/270384

Overexpression of nicotinamide N-methyltransferase in HSC-2 OSCC cell line: effect on apoptosis and cell proliferation
CLINICAL ORAL INVESTIGATIONS
Autore/i: Seta, R; Mascitti, M; Campagna, R; Sartini, D; Fumarola, S; Santarelli, A; Giuliani, M; Cecati, M; Muzio, Ll; Emanuelli, M
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/259054

Role of paraoxonase-2 in bladder cancer: effect of shRNA-mediated gene silencing on cell proliferation and tumourigenicity
FEBS OPENBIO
Autore/i: Fumarola, S; Cecati, M; Morresi, C; Sartini, D; Ferretti, G; Bacchetti, T; Emanuelli, M
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/268870

Bisubstrate Inhibitors of Nicotinamide N-Methyltransferase (NNMT) with Enhanced Activity
JOURNAL OF MEDICINAL CHEMISTRY
Autore/i: Gao, Y; van Haren, Mj; Moret, Ee; Rood, Jjm; Sartini, D; Salvucci, Alessia; Emanuelli, M; Craveur, P; Babault, N; Jin, J; Martin, Ni
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/268868

Glycoxidative Stress and Paraoxonase-2 in Intestinal Cells: Effect of Apple Polyphenols - 3rd European Summer School on Nutrigenomics
LIFESTYLE GENOMICS
Autore/i: Bacchetti, Tiziana; Morresi, Camilla; Sartini, Davide; Fumarola, Stefania; Emanuelli, Monica; Ferretti, Gianna
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/260472

NNMT: potential involvement in oral malignant melanoma
MINERVA STOMATOLOGICA
Autore/i: Togni, Lucrezia; Sartini, D; Troiano, G; Lo Muzio, L; Procaccini, M; Emanuelli, M; Santarelli, A
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/259267

Role of paraoxonase-2 in chemoresistance in T24 human bladder cancer cells
Atti del 15° Congresso Nazionale della Federazione Italiana Scienze della Vita (FISV), Roma, 18-21 Settembre 2018
Autore/i: Bacchetti, T; Morresi, C; Cecati, M; Fumarola, S; Sartini, D; Ferretti, G; Emanuelli, M
Classificazione: 4 Contributo in Atti di Convegno (Proceeding)
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/259340

Expression of nicotinamide N-methyltransferase in oral malignant melanoma
Atti del 15° Congresso Nazionale della Federazione Italiana Scienze della Vita (FISV), Roma, 18-21 Settembre 2018
Autore/i: Mascitti, M; Santarelli, A; Rubini, C; Salvolini, E; Pompei, Veronica; Sartini, D; Emanuelli, M
Classificazione: 4 Contributo in Atti di Convegno (Proceeding)
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/259339

Involvement of transforming growth factor beta 1 in the transcriptional regulation of nicotinamide N-methyltransferase in clear cell renal cell carcinoma
CELLULAR AND MOLECULAR BIOLOGY
Autore/i: Campagna, R; Cecati, M; Pozzi, V; Fumarola, S; Pompei, V; Milanese, G; Galosi, Ab; Sartini, D; Emanuelli, M
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/259055

Clinical performance and utility of a NNMT-based urine test for bladder cancer
THE INTERNATIONAL JOURNAL OF BIOLOGICAL MARKERS
Autore/i: Pozzi, Valentina; DI RUSCIO, Giulia; Sartini, Davide; Campagna, Roberto; Seta, Riccardo; Fulvi, Paola; Vici, A; Milanese, G; Brandoni, G; Galosi, Andrea Benedetto; Montironi, Rodolfo; Cecati, Monia; Emanuelli, Monica
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/251978

Nicotinamide N-methyltransferase: potential involvement in cutaneous malignant melanoma
MELANOMA RESEARCH
Autore/i: Ganzetti, G; Sartini, D; Campanati, A; Rubini, C; Molinelli, E; Brisigotti, V; Cecati, M; Pozzi, V; Campagna, R; Offidani, A; Emanuelli, M.
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/255050

The kinetic analysis of the N-methylation of 4-phenylpyridine by nicotinamide N-methyltransferase: Evidence for a novel mechanism of substrate inhibition
THE INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY
Autore/i: van Haren, Mj; Thomas, Mg; Sartini, D; Barlow, Dj; Ramsden, Db; Emanuelli, M; Klamt, F; Martin, Ni; Parsons, Rb
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/259048

Expression of extracellular matrix and adhesion proteins in pelvic organ prolapse
CELLULAR AND MOLECULAR BIOLOGY
Autore/i: Cecati, Monia; Corradetti, Alessandra; Sartini, Davide; Pozzi, Valentina; Giannubilo, Stefano R; Saccucci, Franca; Ciavattini, Andrea; Emanuelli, Monica
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/258552

Survivin-Based Treatment Strategies for Squamous Cell Carcinoma
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Autore/i: Santarelli, A; Mascitti, M; Lo Russo, L; Sartini, D; Troiano, G; Emanuelli, M; Lo Muzio, L
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/259050

Effect of nicotinamide N-methyltransferase gene silencing on endothelial bioenergetics.
17th Symposium on Purine and Pyrimidine Metabolism in Man, Gdansk, Poland, 20-24 September 2017
Autore/i: Campagna, R; Mateuszuk, L; Kaczara, P; Sartini, D; Emanuelli, M; Chlopicki, S
Classificazione: 4 Contributo in Atti di Convegno (Proceeding)
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/301252

Diagnostic and prognostic role of nicotinamide N-methyltransferase in cutaneous malignant melanoma
BIOCHIMICA CLINICA
Autore/i: Ganzetti, G; Sartini, D; Campanati, A; Rubini, C; Molinelli, Elisa; Brisigotti, Valerio; Fumarola, Stefania; Cecati, M; Pozzi, V; Campagna, Roberto; Offidani, A; Emanuelli, M.
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/251984

Glycoxidative Stress and Paraoxonase-2 in Intestinal Cells: Effect of Apple Polyphenols
Atti del 59° Congresso Nazionale della Società Italiana di Biochimica e Biologia Molecolare (SIB), Caserta, 20-22 Settembre 2017
Autore/i: Bacchetti, T; Morresi, Camilla; Turco, I; Ferretti, G; Sartini, D; Fumarola, S; Emanuelli, M.
Classificazione: 4 Contributo in Atti di Convegno (Proceeding)
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/251983

Nicotinamide N-methyltransferase: a new potential biomarker for cutaneous malignant melanoma
Atti del 59° Congresso Nazionale della Società Italiana di Biochimica e Biologia Molecolare (SIB), Caserta, 20-22 Settembre 2017
Autore/i: Ganzetti, G; Sartini, D; Campanati, A; Molinelli, E; Brisigotti, V; Cecati, M; Pozzi, V; Campagna, R; Fumarola, S; Rubini, C; Offidani, A; Emanuelli, M.
Classificazione: 4 Contributo in Atti di Convegno (Proceeding)
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/251981

Nicotinamide N-methyltransferase gene silencing in endothelial cell lines: molecular and phenotypic characterization
Atti del 25th Krakow Conference on Endothelium, 20th-21st October 2017, Krakow
Autore/i: Campagna, R; Mateuszuk, L; Kaczara, P; Sartini, D; Cecati, M; Fumarola, S; Emanuelli, M; Chlopicki, STEFAN KAROL
Classificazione: 4 Contributo in Atti di Convegno (Proceeding)
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/251985

The TDH-GCN5L1-Fbxo15-KBP axis limits mitochondrial biogenesis in mouse embryonic stem cells
NATURE CELL BIOLOGY
Autore/i: Donato, V; Bonora, M; Simoneschi, D; Sartini, Davide; Kudo, Y; Saraf, A; Florens, L; Washburn, Mp; Stadtfeld, M; Pinton, P; Pagano, M.
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/251128

Exploring the role of paraoxonase-2 in bladder cancer: analyses performed on tissue samples, urines and cell cultures
ONCOTARGET
Autore/i: Bacchetti, Tiziana; Sartini, Davide; Pozzi, Valentina; Cacciamani, Tiziana; Ferretti, Gianna; Emanuelli, Monica
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/246589

Nuclear Survivin as a Prognostic Factor in Squamous-Cell Carcinoma of the Oral Cavity
APPLIED IMMUNOHISTOCHEMISTRY AND MOLECULAR MORPHOLOGY
Autore/i: Santarelli, Andrea; Mascitti, M; Rubini, Corrado; Bambini, Fabrizio; Giannatempo, G; Lo Russo, L; Sartini, Davide; Emanuelli, Monica; Procaccini, Maurizio; Lo Muzio, L.
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/236729

Identification of paraoxonase-2 as potential biomarker for bladder cancer
ANTICANCER RESEARCH
Autore/i: Marsili, Chiara; Ferretti, Gianna; Sartini, Davide; Milanese, Giulio; Galosi, Andrea Benedetto; Emanuelli, Monica; Bacchetti, Tiziana
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/246590

Molecular analysis of endometrial inflammation in preterm birth
CELLULAR AND MOLECULAR BIOLOGY
Autore/i: Cecati, Monia; Sartini, Davide; Campagna, Roberto; Biagini, Alessandra; Ciavattini, Andrea; Emanuelli, Monica; Giannubilo, Stefano Raffaele
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/246588

Use of supercharged cover screw as static magnetic field generator for bone healing, 2nd part: in vivo enhancement of bone regeneration in rabbits
JOURNAL OF BIOLOGICAL REGULATORS & HOMEOSTATIC AGENTS
Autore/i: Bambini, F; Santarelli, A; Putignano, A; Procaccini, M; Orsini, G; Di Iorio, D; Memè, L; Sartini, D; Emanuelli, M; Lo Muzio, L.
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/251976

Inhibitors of nicotinamide N-methyltransferase designed to mimic the methylation reaction transition state
ORGANIC & BIOMOLECULAR CHEMISTRY
Autore/i: van Haren, Mj; Taig, R; Kuppens, J; Sastre Toraño, J; Moret, Ee; Parsons, Rb; Sartini, D; Emanuelli, M; Martin, Ni
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/251977

Use of supercharged cover screw as static magnetic field generator for bone healing, 1st part: in vitro enhancement of osteoblast-like cell differentiation
JOURNAL OF BIOLOGICAL REGULATORS & HOMEOSTATIC AGENTS
Autore/i: Bambini, Fabrizio; Santarelli, Andrea; Putignano, Angelo; Procaccini, Maurizio; Orsini, Giovanna; Meme', Lucia; Sartini, Davide; Emanuelli, Monica; Lo Muzio, L.
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/246090

Overexpression of Nicotinamide N-Methyltransferase in HSC-2 OSCC cell line: effect on apoptosis and cell proliferation
MINERVA STOMATOLOGICA
Autore/i: Giannatempo, G; Mascitti, M; Di Fede, O; Mauceri, R; Santarelli, Andrea; Seta, Riccardo; Sartini, Davide; Campagna, Roberto; Emanuelli, Monica; Lo Muzio, L.
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/237199

Molecular and phenotypic characterization of cancer stem cells from human tumor cell lines
14° Congresso Nazionale della Federazione Italiana Scienze della Vita (FISV), Roma, 20-23 Settembre 2016
Autore/i: Sartini, Davide; Pozzi, Valentina; Seta, Riccardo; Campagna, Roberto; Fumarola, S; Pompei, V; Cecati, Monia; Santarelli, Andrea; Lo Muzio, L; Emanuelli, Monica
Classificazione: 4 Contributo in Atti di Convegno (Proceeding)
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/240432

Cancer stem cells from human tumor cell lines: molecular and phenotypic features
BIOCHIMICA CLINICA
Autore/i: Campagna, Roberto; Sartini, Davide; Pozzi, Valentina; Seta, Riccardo; Fumarola, S; Pompei, V; Cecati, Monia; Santarelli, Andrea; Lo Muzio, L; Emanuelli, Monica
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/240420

Characterization of cancer stem cells from larynx and bladder carcinoma cell lines
1st Young Scientist Workshop on “Stem Cell Niche: from Basic Science to Clinical Application”, 8-10 May 2016, Pavia
Autore/i: Sartini, Davide; Pozzi, Valentina; Seta, Riccardo; DI RUSCIO, Giulia; Campagna, Roberto; Emanuelli, Monica
Classificazione: 4 Contributo in Atti di Convegno (Proceeding)
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/237202

Salivary interleukin-1β: Oral inflammatory biomarker in patients with psoriasis.
JOURNAL OF INTERNATIONAL MEDICAL RESEARCH
Autore/i: Ganzetti, Giulia; Campanati, Anna; Santarelli, Andrea; Sartini, Davide; Molinelli, Elisa; Brisigotti, Valerio; DI RUSCIO, Giulia; Bobyr, Ivan; Emanuelli, Monica; Offidani, Annamaria
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/239517

Nicotinamide N-methyltransferase catalyses the N-methylation of the endogenous β-carboline norharman: evidence for a novel detoxification pathway
BIOCHEMICAL JOURNAL
Autore/i: Thomas, Mg; Sartini, Davide; Emanuelli, Monica; van Haren, Mj; Martin, Ni; Mountford, Dm; Barlow, Dj; Klamt, F; Ramsden, Db; Reza, M; Parsons, Rb
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/236731

Betacyanins enhance vitexin-2-O-xyloside mediated inhibition of proliferation of T24 bladder cancer cells
FOOD & FUNCTION
Autore/i: Scarpa, Es; Emanuelli, Monica; Frati, A; Pozzi, Valentina; Antonini, E; Diamantini, G; DI RUSCIO, Giulia; Sartini, Davide; Armeni, Tatiana; Palma, F; Ninfali, P.
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/240407

Potential Role of Placental Klotho in the Pathogenesis of Preeclampsia.
CELL BIOCHEMISTRY AND BIOPHYSICS
Autore/i: Cecati, Monia; Giannubilo, Stefano Raffaele; Saccucci, Franca; Sartini, Davide; Ciavattini, Andrea; Emanuelli, Monica; Tranquilli, Andrea Luigi
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/234649

A rapid and efficient assay for the characterization of substrates and inhibitors of nicotinamide N-methyltransferase
BIOCHEMISTRY
Autore/i: van Haren, Mj; Sastre Torano, J; Sartini, Davide; Emanuelli, Monica; Parsons, Rb; Martin, Ni
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/236721

Cancer stem cells from head and neck squamous cell carcinoma
ANNALI DI STOMATOLOGIA
Autore/i: Dioguardi, M; Sartini, Davide; Santarelli, Andrea; Lo Muzio, L; Emanuelli, Monica
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/237197

Paraoxonase 2 (PON2) expression in bladder cancer
12th International Meeting on Cholinesterases and 6th International Conference on Paraoxonases, Alicante, Spagna, 27 Settembre - 2 Ottobre 2015
Autore/i: Bacchetti, Tiziana; Ferretti, Gianna; Giuliante, Rachela; Sartini, Davide; Pozzi, Valentina; Emanuelli, Monica
Classificazione: 4 Contributo in Atti di Convegno (Proceeding)
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/237193

Natural extracts from the genus Beta and their cytotoxic activity on T24 human bladder cancer cells
58° Congresso Nazionale della Società Italiana di Biochimica e Biologia Molecolare (SIB), Urbino, 14-16 Settembre 2015
Autore/i: Emanuelli, Monica; Frati, A; DI RUSCIO, Giulia; Scarpa, Es; Pozzi, Valentina; Giuliante, Rachela; Sartini, Davide; Ninfali, P.
Classificazione: 4 Contributo in Atti di Convegno (Proceeding)
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/237185

Upregulation of placental endothelial Nitric Oxide Synthase (eNOS) in obese pregnant women: a case-control study
58° Congresso Nazionale della Società Italiana di Biochimica e Biologia Molecolare (SIB), Urbino, 14-16 Settembre 2015
Autore/i: Giulietti, Alessia; Sabbatinelli, Jacopo; Vignini, Arianna; DI RUSCIO, Giulia; Sartini, Davide; Mazzanti, Laura; Emanuelli, Monica
Classificazione: 4 Contributo in Atti di Convegno (Proceeding)
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/237189

Enhanced expression of paraoxonase 2 in human bladder cancer
58° Congresso Nazionale della Società Italiana di Biochimica e Biologia Molecolare (SIB), Urbino, 14-16 Settembre 2015
Autore/i: Bacchetti, Tiziana; Ferretti, Gianna; Giuliante, Rachela; Sartini, Davide; Seta, Riccardo; Pozzi, Valentina; Emanuelli, Monica
Classificazione: 4 Contributo in Atti di Convegno (Proceeding)
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/237181

Pokemon proto-oncogene in oral cancer: potential role in the early phase of tumorigenesis.
ORAL DISEASES
Autore/i: Sartini, Davide; Lo Muzio, L; Morganti, Stefano; Pozzi, Valentina; DI RUSCIO, Giulia; Rocchetti, Romina; Rubini, Corrado; Santarelli, Andrea; Emanuelli, Monica
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/229536

Identification and Characterization of Cancer Stem Cells from Head and Neck Squamous Cell Carcinoma Cell Lines
CELLULAR PHYSIOLOGY AND BIOCHEMISTRY
Autore/i: Pozzi, Valentina; Sartini, Davide; Rocchetti, Romina; Santarelli, Andrea; Rubini, Corrado; Morganti, Stefano; Giuliante, Rachela; Calabrese, S; DI RUSCIO, Giulia; Orlando, Fiorenza; Provinciali, M; Saccucci, Franca; Lo Muzio, L; Emanuelli, Monica
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/229030

Role of nicotinamide N-methyltransferase in non-small cell lung cancer: in vitro effect of shRNA-mediated gene silencing on tumorigenicity
Biological chemistry
Autore/i: Sartini, D; Seta, R; Pozzi, V; Morganti, S; Rubini, C; Zizzi, A; Tomasetti, M; Santarelli, L; Emanuelli, M.
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/201706

Potential involvement of nicotinamide N-methyltransferase in the pathogenesis of metabolic syndrome
METABOLIC SYNDROME AND RELATED DISORDERS
Autore/i: Giuliante, R; Sartini, D; Bacchetti, T; Rocchetti, R; Klöting, I; Polidori, C; Ferretti, G; Emanuelli, M.
Classificazione: 1 Contributo su Rivista
Scheda della pubblicazione: https://iris.univpm.it/handle/11566/233406